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The map label for this gene is czcB [H]

Identifier: 37521599

GI number: 37521599

Start: 2178892

End: 2180352

Strand: Direct

Name: czcB [H]

Synonym: glr2030

Alternate gene names: 37521599

Gene position: 2178892-2180352 (Clockwise)

Preceding gene: 37521598

Following gene: 37521600

Centisome position: 46.77

GC content: 68.72

Gene sequence:

>1461_bases
ATGTCCAAGGCCATGTTCGGTATACGAATCGTCGGGTCGGCCGCTCTGCTAGCCGGAGTGCTTTATGCCACCCCGGTCCT
GGCCCATGGCAACCACGGCGATAAGGAATTTGCCGGAAACAAGCCAGCGGCGGTTGCAGGCGAGCAGGTGAAGGTAGCCC
CCGACGTGCAAAAGGCGCTCGGGCTTGCCGTCACGGCTGTGGGCGAGCAGGTGTTGCGCGCAGGTTTCGAGGCGAACGGC
CGCATCGAGGCGATTCCTGGCCGCAGTGCCGAGATCCTGGCTCCCGTGGCCGGTCGAGTCTTGCGGCTGGTCGCCTCGCG
CGGGCAGGCGGTGCGCCGGGGTGAAACCTTACTGGTGCTCGACAGCTCCGAGATTCGCGCCCTCGCTGTGCAGAGCGAGC
AGGCAAAAGCCGCCGCCCGCGCCGAGTCGAAGCAGGCCGAGGCCCGGCTGCGGCTGGCGAGGAGCACCTTCGAGCGCGAA
AAGGAACTGGTGGATCTGAAGATTTCGGCGCGCAAAGATTTTCAGGTGGCCCAATCCGAGTACGAGCAGGCGGCAGCCGA
TCTCGAAGCAGTCAGAGCGCGTCTGAGGCTGAGCGGGGCGCAACTGGACGCCCGCCTCGCCCAACTCGGACAGCGCGGGG
TGCAGGCGGCCCGCGACGGCCGCGTCACCGTCGCTTCTGCGGTGAGCGGCTTCGTGGCGCAGCAGAGCGTCAGTGCCGGG
GAGGCGGTTGAAGCGGGGACGCCCCTGTACCGGGTGGTGGACACCGCCGGTGGAGTCTGGGCGACGGCGCAGATCTACGA
ACAAGACCTGGCGCAGGTGCGGGTGGGCCGGCCGGTGGAAGTGGTGACGGCCGCCTACCCGGGCCAGGTCTTCAAAGGCC
GTGTGGACAGTGTCGATCCTGCCGTCGATCCCGAGACGCGCACGCTTGCGGTGCGGGCGGTGCTCGCCAACCCCGGCGGC
CGTCTCAAGCCCGAGATGTTCGCCACCCTGCGCGTGACGACCAGCGACCGCCCGGCTGCTGTATCGGTCATTCCCCGCTC
CGCCGTGGTCGAGGCGGATGGGGCGCGGATCGTCTATGTCCAGAACGGTCCCGACGTCTTTGAGCCGGTGGAGGTGGATC
TGGGTCGGCTAAGCGGCGAACTGATTGAAGTCAAAAGCGGTGTCTATCCGGGCGACCTGGTCGTGAGCCGCCGCGCTTTT
CAGCTACGTGCCCAGGCGCTCAAGGGCGGCATCCCGGCTGAAGACGGCGCCTCCGGCGAGATCGAATCGGAAAAGCCAAA
GCCCGAGAGTCCGTCACCGGGCGGTACGCCCTGGTGGGCGCTCACCTTTGGGGCTATCGGGTTGGCCGGTGTCGCTTTTT
GGGCCGGGGCGCGCTTTGCGGCGGGGCGCGTCGCCGGTGTCGGCCTGGGCGCCGGGGAGGGGGCTCCGCCGCATCGCTCC
GCCGCCCGTCCGCGCCGCTAG

Upstream 100 bases:

>100_bases
GACCCGCAACCGCTACTACCAGTCGATCCTCGAATACCAGACGGCCCTGGCCGATCTGGAAAAGGCGATCGGTGTTCCCC
TCACCACCTGACGAGGCACC

Downstream 100 bases:

>100_bases
GGGGCTCCCAGAATGCTCAACCAACTGATTTTCTGGTCGATCGTCCAGCGCTGGCTGGTGGTGCTCGCCTCGGTGCTGCT
GCTGGTGGTGGGTACCGCCG

Product: nickel-cobalt resistance protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 486; Mature: 485

Protein sequence:

>486_residues
MSKAMFGIRIVGSAALLAGVLYATPVLAHGNHGDKEFAGNKPAAVAGEQVKVAPDVQKALGLAVTAVGEQVLRAGFEANG
RIEAIPGRSAEILAPVAGRVLRLVASRGQAVRRGETLLVLDSSEIRALAVQSEQAKAAARAESKQAEARLRLARSTFERE
KELVDLKISARKDFQVAQSEYEQAAADLEAVRARLRLSGAQLDARLAQLGQRGVQAARDGRVTVASAVSGFVAQQSVSAG
EAVEAGTPLYRVVDTAGGVWATAQIYEQDLAQVRVGRPVEVVTAAYPGQVFKGRVDSVDPAVDPETRTLAVRAVLANPGG
RLKPEMFATLRVTTSDRPAAVSVIPRSAVVEADGARIVYVQNGPDVFEPVEVDLGRLSGELIEVKSGVYPGDLVVSRRAF
QLRAQALKGGIPAEDGASGEIESEKPKPESPSPGGTPWWALTFGAIGLAGVAFWAGARFAAGRVAGVGLGAGEGAPPHRS
AARPRR

Sequences:

>Translated_486_residues
MSKAMFGIRIVGSAALLAGVLYATPVLAHGNHGDKEFAGNKPAAVAGEQVKVAPDVQKALGLAVTAVGEQVLRAGFEANG
RIEAIPGRSAEILAPVAGRVLRLVASRGQAVRRGETLLVLDSSEIRALAVQSEQAKAAARAESKQAEARLRLARSTFERE
KELVDLKISARKDFQVAQSEYEQAAADLEAVRARLRLSGAQLDARLAQLGQRGVQAARDGRVTVASAVSGFVAQQSVSAG
EAVEAGTPLYRVVDTAGGVWATAQIYEQDLAQVRVGRPVEVVTAAYPGQVFKGRVDSVDPAVDPETRTLAVRAVLANPGG
RLKPEMFATLRVTTSDRPAAVSVIPRSAVVEADGARIVYVQNGPDVFEPVEVDLGRLSGELIEVKSGVYPGDLVVSRRAF
QLRAQALKGGIPAEDGASGEIESEKPKPESPSPGGTPWWALTFGAIGLAGVAFWAGARFAAGRVAGVGLGAGEGAPPHRS
AARPRR
>Mature_485_residues
SKAMFGIRIVGSAALLAGVLYATPVLAHGNHGDKEFAGNKPAAVAGEQVKVAPDVQKALGLAVTAVGEQVLRAGFEANGR
IEAIPGRSAEILAPVAGRVLRLVASRGQAVRRGETLLVLDSSEIRALAVQSEQAKAAARAESKQAEARLRLARSTFEREK
ELVDLKISARKDFQVAQSEYEQAAADLEAVRARLRLSGAQLDARLAQLGQRGVQAARDGRVTVASAVSGFVAQQSVSAGE
AVEAGTPLYRVVDTAGGVWATAQIYEQDLAQVRVGRPVEVVTAAYPGQVFKGRVDSVDPAVDPETRTLAVRAVLANPGGR
LKPEMFATLRVTTSDRPAAVSVIPRSAVVEADGARIVYVQNGPDVFEPVEVDLGRLSGELIEVKSGVYPGDLVVSRRAFQ
LRAQALKGGIPAEDGASGEIESEKPKPESPSPGGTPWWALTFGAIGLAGVAFWAGARFAAGRVAGVGLGAGEGAPPHRSA
ARPRR

Specific function: CzcA and CzcB together would act in zinc efflux nearly as effectively as the complete czc efflux system (CzcABC). The CzcB protein is thought to funnel zinc cations to the CzcA transport protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI87081796, Length=331, Percent_Identity=25.0755287009063, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789929, Length=325, Percent_Identity=25.2307692307692, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1789041, Length=240, Percent_Identity=25.8333333333333, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005695
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 50750; Mature: 50619

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKAMFGIRIVGSAALLAGVLYATPVLAHGNHGDKEFAGNKPAAVAGEQVKVAPDVQKAL
CCCCEEEEEEHHHHHHHHHHHHHHHHEECCCCCCHHHCCCCCCEECCCCEEECCCHHHHH
GLAVTAVGEQVLRAGFEANGRIEAIPGRSAEILAPVAGRVLRLVASRGQAVRRGETLLVL
HHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCEEEEE
DSSEIRALAVQSEQAKAAARAESKQAEARLRLARSTFEREKELVDLKISARKDFQVAQSE
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHH
YEQAAADLEAVRARLRLSGAQLDARLAQLGQRGVQAARDGRVTVASAVSGFVAQQSVSAG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCC
EAVEAGTPLYRVVDTAGGVWATAQIYEQDLAQVRVGRPVEVVTAAYPGQVFKGRVDSVDP
CHHHCCCCCEEEHHCCCCCEEHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCCCCCCCC
AVDPETRTLAVRAVLANPGGRLKPEMFATLRVTTSDRPAAVSVIPRSAVVEADGARIVYV
CCCCCCCEEEEEEEEECCCCCCCCCEEEEEEEECCCCCCEEEEECCCEEEECCCCEEEEE
QNGPDVFEPVEVDLGRLSGELIEVKSGVYPGDLVVSRRAFQLRAQALKGGIPAEDGASGE
CCCCCCCCCHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
IESEKPKPESPSPGGTPWWALTFGAIGLAGVAFWAGARFAAGRVAGVGLGAGEGAPPHRS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCCCCCC
AARPRR
CCCCCC
>Mature Secondary Structure 
SKAMFGIRIVGSAALLAGVLYATPVLAHGNHGDKEFAGNKPAAVAGEQVKVAPDVQKAL
CCCEEEEEEHHHHHHHHHHHHHHHHEECCCCCCHHHCCCCCCEECCCCEEECCCHHHHH
GLAVTAVGEQVLRAGFEANGRIEAIPGRSAEILAPVAGRVLRLVASRGQAVRRGETLLVL
HHHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCEEEEE
DSSEIRALAVQSEQAKAAARAESKQAEARLRLARSTFEREKELVDLKISARKDFQVAQSE
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCHHHHHHH
YEQAAADLEAVRARLRLSGAQLDARLAQLGQRGVQAARDGRVTVASAVSGFVAQQSVSAG
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHCCCCC
EAVEAGTPLYRVVDTAGGVWATAQIYEQDLAQVRVGRPVEVVTAAYPGQVFKGRVDSVDP
CHHHCCCCCEEEHHCCCCCEEHHHHHHHHHHHHHCCCCEEEEEECCCCHHHCCCCCCCCC
AVDPETRTLAVRAVLANPGGRLKPEMFATLRVTTSDRPAAVSVIPRSAVVEADGARIVYV
CCCCCCCEEEEEEEEECCCCCCCCCEEEEEEEECCCCCCEEEEECCCEEEECCCCEEEEE
QNGPDVFEPVEVDLGRLSGELIEVKSGVYPGDLVVSRRAFQLRAQALKGGIPAEDGASGE
CCCCCCCCCHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
IESEKPKPESPSPGGTPWWALTFGAIGLAGVAFWAGARFAAGRVAGVGLGAGEGAPPHRS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCHHHCCCEEEEECCCCCCCCCCCC
AARPRR
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8829543 [H]