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The map label for this gene is sir [H]

Identifier: 37521417

GI number: 37521417

Start: 1967874

End: 1969556

Strand: Direct

Name: sir [H]

Synonym: gvip254

Alternate gene names: 37521417

Gene position: 1967874-1969556 (Clockwise)

Preceding gene: 37521410

Following gene: 37521418

Centisome position: 42.24

GC content: 65.72

Gene sequence:

>1683_bases
ATGACCGATACGATGCGGCGCTCGAAAGTCGAGCAAATCAAAGAGAACAGCCACAATCTGCGCGGCCGGCTGCGCGAAGA
GTTGCAGCAGCCCGAGACGACGCACTTTGCCGAGGAGTCGGTGCAGCTGCTCAAATTCCACGGCATCTACCAGCAGGACG
ACCGCGACGAGCGCAAGGCGCGCAAGGACGCGGGCCTGGAGCCCGATTACGCGTTCATGGTACGCACCAAAAATCCGGGC
GGCTACGCCCCGGCAGCCTTTTACCTGGCGATGGACCGGTTGGCCGACGCCCTCGGCAGCGGCACCCTGCGGGTGACCAC
CCGCCAGGGCCTGCAGTTGCACGGCATCCGCAAACACAACCTGCGCGAGGCGATTGCCACGATTAGCACCCAACTCGGTT
CGACCCTGGGAGCCTGCGGCGACATCAACCGCAACGTCATGGCCCCCCCGGCGCCCTTCACCGCCCGCCCCTACGCCGTG
GCGCGCGAGGCGGCGGCGGCGATCGCCGATATGCTCACCCCGCACACGGGCGCCTACTTTGAGGTGTGGCAGGACGAAGC
GCTGGTCTATTCGGGAGAGCCCGAGGTCGAGCCGATTTACGGCGAGACTTACCTGCCGCGCAAGTTCAAAATTGCCGTCG
CCGTCGAAGGCGACAACTCCGTGGACATCTACACCCAGGATCTGGGGGTGATCCCGGTGTTCGAGGACGGCGAGCGCTTG
TTGGGCTACAACCTCACCGTCGGGGGCGGCCTCGGCATGACCCACGCTAAACCCGAGACCTTTCCGCGCCAGGCGGACCA
CCTGGGTTTTGTTTCCTCCGAGGACATGCTGGAGGCGGTCAAAGCGGTGGTGCTGGTGCAGCGCGACCACGGCGACCGCT
ACAACCGTCGCCACGCCCGGCTGAAATATCTCATCCACGACCGCGGCCTGGGCTGGTTCCGCGCCCAGGTGGAAACTTAC
CTGGGCAAACCCCTGCAACCGTGGCGGACCGTGGCGCCGTGGGTCTTTCACGATTATTTGGGCTGGCAGCCCCAGGGGGA
CGGCCGCTACTGCCTGGGGATCTGGATCGAGAATGGCCGCATCAAAGACGAGGGAGCCTTTCGCCTCAAATCGGCCCTGC
GCGAGATCGTCGAGCGCTTTGAGGTGGATTTGATCTTGACCCCGACCCAGAATGCGCTGCTGGTGGATGTCGCCCCGGAG
GTACGTGCACCCATCGACGCGATTTTGCAAAGCGCCGGGGTGCGGCCGGTTCAGGCCGTCTCCAACGCCGAGCGCTACGC
GATGGCCTGTCCCGCCTGGCCCACCTGCGGTCTGGCCCTCACCGAGTCGGAGCGCGCCCTGCCCGGGATCATCGCCGAGC
TCGAGGCGAAACTCACCGAGCTGGACCTCGCGCAGGAGCAGATTTCGATTCGCATGACCGGCTGCCCGAACGGTTGCGCC
CGCCCCTACATGGGCGAAATTGGTTTCGTGGGCAGCGCCCCCGGTGCCTATAACCTCTACCTGGGGGGCAACCTCGCCTC
GACGCGGCTCAACTGGGTCTTCCGCGAGCGGGTCAAGCGCGAGGATATCCTGGCGGTGCTCGGGCCGCTGTTCAGCTACT
TCAAGCGCGAGCGCGCCCCGGGGGAAAGCTTCGGCGATTTTTGCCTGCGCAAGGGCCGGGCGGACCTCGAAGCGCATCTC
TAG

Upstream 100 bases:

>100_bases
CCGCCCAAGGCTTATCCTGTTTTTGCATGGGGTGTGAGCAGCTTCACATGACAAACGCTCGAATAATTGCTAAATCTCGA
TAGAGATACCGGGCAAGGCG

Downstream 100 bases:

>100_bases
GTCGGCGCTTTGGCTTTGACGCCGCCCCTGGTGCTTGCTCTGTTTGCCCTCGGCTGCATTTTGCTGTGGCTGCCGGTGGC
GATCCCACAGCTGCTGCGCA

Product: sulfite reductase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 560; Mature: 559

Protein sequence:

>560_residues
MTDTMRRSKVEQIKENSHNLRGRLREELQQPETTHFAEESVQLLKFHGIYQQDDRDERKARKDAGLEPDYAFMVRTKNPG
GYAPAAFYLAMDRLADALGSGTLRVTTRQGLQLHGIRKHNLREAIATISTQLGSTLGACGDINRNVMAPPAPFTARPYAV
AREAAAAIADMLTPHTGAYFEVWQDEALVYSGEPEVEPIYGETYLPRKFKIAVAVEGDNSVDIYTQDLGVIPVFEDGERL
LGYNLTVGGGLGMTHAKPETFPRQADHLGFVSSEDMLEAVKAVVLVQRDHGDRYNRRHARLKYLIHDRGLGWFRAQVETY
LGKPLQPWRTVAPWVFHDYLGWQPQGDGRYCLGIWIENGRIKDEGAFRLKSALREIVERFEVDLILTPTQNALLVDVAPE
VRAPIDAILQSAGVRPVQAVSNAERYAMACPAWPTCGLALTESERALPGIIAELEAKLTELDLAQEQISIRMTGCPNGCA
RPYMGEIGFVGSAPGAYNLYLGGNLASTRLNWVFRERVKREDILAVLGPLFSYFKRERAPGESFGDFCLRKGRADLEAHL

Sequences:

>Translated_560_residues
MTDTMRRSKVEQIKENSHNLRGRLREELQQPETTHFAEESVQLLKFHGIYQQDDRDERKARKDAGLEPDYAFMVRTKNPG
GYAPAAFYLAMDRLADALGSGTLRVTTRQGLQLHGIRKHNLREAIATISTQLGSTLGACGDINRNVMAPPAPFTARPYAV
AREAAAAIADMLTPHTGAYFEVWQDEALVYSGEPEVEPIYGETYLPRKFKIAVAVEGDNSVDIYTQDLGVIPVFEDGERL
LGYNLTVGGGLGMTHAKPETFPRQADHLGFVSSEDMLEAVKAVVLVQRDHGDRYNRRHARLKYLIHDRGLGWFRAQVETY
LGKPLQPWRTVAPWVFHDYLGWQPQGDGRYCLGIWIENGRIKDEGAFRLKSALREIVERFEVDLILTPTQNALLVDVAPE
VRAPIDAILQSAGVRPVQAVSNAERYAMACPAWPTCGLALTESERALPGIIAELEAKLTELDLAQEQISIRMTGCPNGCA
RPYMGEIGFVGSAPGAYNLYLGGNLASTRLNWVFRERVKREDILAVLGPLFSYFKRERAPGESFGDFCLRKGRADLEAHL
>Mature_559_residues
TDTMRRSKVEQIKENSHNLRGRLREELQQPETTHFAEESVQLLKFHGIYQQDDRDERKARKDAGLEPDYAFMVRTKNPGG
YAPAAFYLAMDRLADALGSGTLRVTTRQGLQLHGIRKHNLREAIATISTQLGSTLGACGDINRNVMAPPAPFTARPYAVA
REAAAAIADMLTPHTGAYFEVWQDEALVYSGEPEVEPIYGETYLPRKFKIAVAVEGDNSVDIYTQDLGVIPVFEDGERLL
GYNLTVGGGLGMTHAKPETFPRQADHLGFVSSEDMLEAVKAVVLVQRDHGDRYNRRHARLKYLIHDRGLGWFRAQVETYL
GKPLQPWRTVAPWVFHDYLGWQPQGDGRYCLGIWIENGRIKDEGAFRLKSALREIVERFEVDLILTPTQNALLVDVAPEV
RAPIDAILQSAGVRPVQAVSNAERYAMACPAWPTCGLALTESERALPGIIAELEAKLTELDLAQEQISIRMTGCPNGCAR
PYMGEIGFVGSAPGAYNLYLGGNLASTRLNWVFRERVKREDILAVLGPLFSYFKRERAPGESFGDFCLRKGRADLEAHL

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=549, Percent_Identity=37.8870673952641, Blast_Score=384, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6322597, Length=576, Percent_Identity=40.7986111111111, Blast_Score=433, Evalue=1e-122,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR011787 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.8.7.1 [H]

Molecular weight: Translated: 62486; Mature: 62355

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDTMRRSKVEQIKENSHNLRGRLREELQQPETTHFAEESVQLLKFHGIYQQDDRDERKA
CCCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHH
RKDAGLEPDYAFMVRTKNPGGYAPAAFYLAMDRLADALGSGTLRVTTRQGLQLHGIRKHN
HHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCHHHH
LREAIATISTQLGSTLGACGDINRNVMAPPAPFTARPYAVAREAAAAIADMLTPHTGAYF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEE
EVWQDEALVYSGEPEVEPIYGETYLPRKFKIAVAVEGDNSVDIYTQDLGVIPVFEDGERL
EEECCCEEEECCCCCCCEECCCCCCCCEEEEEEEEECCCCEEEEEECCCEEEEECCCCEE
LGYNLTVGGGLGMTHAKPETFPRQADHLGFVSSEDMLEAVKAVVLVQRDHGDRYNRRHAR
EEEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHH
LKYLIHDRGLGWFRAQVETYLGKPLQPWRTVAPWVFHDYLGWQPQGDGRYCLGIWIENGR
EEEEEECCCCCHHHHHHHHHHCCCCCCHHHHCCHHHHHHCCCCCCCCCCEEEEEEEECCE
IKDEGAFRLKSALREIVERFEVDLILTPTQNALLVDVAPEVRAPIDAILQSAGVRPVQAV
ECCCHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCHHHCCHHHHHHHCCCCHHHHH
SNAERYAMACPAWPTCGLALTESERALPGIIAELEAKLTELDLAQEQISIRMTGCPNGCA
CCCHHHEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCC
RPYMGEIGFVGSAPGAYNLYLGGNLASTRLNWVFRERVKREDILAVLGPLFSYFKRERAP
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GESFGDFCLRKGRADLEAHL
CCCHHHHHHHCCCCCHHCCC
>Mature Secondary Structure 
TDTMRRSKVEQIKENSHNLRGRLREELQQPETTHFAEESVQLLKFHGIYQQDDRDERKA
CCHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHH
RKDAGLEPDYAFMVRTKNPGGYAPAAFYLAMDRLADALGSGTLRVTTRQGLQLHGIRKHN
HHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCEEECCHHHH
LREAIATISTQLGSTLGACGDINRNVMAPPAPFTARPYAVAREAAAAIADMLTPHTGAYF
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEE
EVWQDEALVYSGEPEVEPIYGETYLPRKFKIAVAVEGDNSVDIYTQDLGVIPVFEDGERL
EEECCCEEEECCCCCCCEECCCCCCCCEEEEEEEEECCCCEEEEEECCCEEEEECCCCEE
LGYNLTVGGGLGMTHAKPETFPRQADHLGFVSSEDMLEAVKAVVLVQRDHGDRYNRRHAR
EEEEEEECCCCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHEEEECCCCCCHHHHHHH
LKYLIHDRGLGWFRAQVETYLGKPLQPWRTVAPWVFHDYLGWQPQGDGRYCLGIWIENGR
EEEEEECCCCCHHHHHHHHHHCCCCCCHHHHCCHHHHHHCCCCCCCCCCEEEEEEEECCE
IKDEGAFRLKSALREIVERFEVDLILTPTQNALLVDVAPEVRAPIDAILQSAGVRPVQAV
ECCCHHHHHHHHHHHHHHHHCCCEEEECCCCEEEEEECCHHHCCHHHHHHHCCCCHHHHH
SNAERYAMACPAWPTCGLALTESERALPGIIAELEAKLTELDLAQEQISIRMTGCPNGCA
CCCHHHEEECCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHEEEEEEEECCCCCCC
RPYMGEIGFVGSAPGAYNLYLGGNLASTRLNWVFRERVKREDILAVLGPLFSYFKRERAP
CCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
GESFGDFCLRKGRADLEAHL
CCCHHHHHHHCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8347657 [H]