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The map label for this gene is cobQ

Identifier: 37521341

GI number: 37521341

Start: 1881850

End: 1883322

Strand: Direct

Name: cobQ

Synonym: gvip245

Alternate gene names: 37521341

Gene position: 1881850-1883322 (Clockwise)

Preceding gene: 37521340

Following gene: 37521342

Centisome position: 40.39

GC content: 62.86

Gene sequence:

>1473_bases
GTGGCTGCTTTGATGGTCGTCGGCACTTCCTCTCACGCGGGCAAGTCACTTCTGGTGACGGCGCTGGGTCGCCTGTTTCA
CCGGCGCGGGGTAAAAGTCGCTCCCTTCAAAGGCCAGAATATGGCCCTCAACGCCTATGTCACCGCCGAAGGCCACGAAA
TCGGCCACGCCCAGGCGGTGCAGGCCTGGGCCTGCGGACTGGAACCCAGCGTGGCGATGAACCCGATTTTGCTCAAGCCC
CAGGGCAATATGACCAGCCAGGTGATCTTGAAGGGCAAGCCCGCCGGGGTGTGCGGGGCCGTCGATTACTACCGCGACTA
CTTTGAACCCGGCTGGCAGGCGGTGGTGGAGGCACTCGCTGAGTTGCAGTCGCAATACGAACTGGTGATCTGCGAAGGAG
CCGGCAGTCCGGCGGAGGTTAACCTGCGCCACCGGGATCTGACCAACATGCGCGTCGCTTTGCACCTGGGGGCGCCGACA
CTGCTGGTGACCGACATCGACCGGGGAGGCGCCCTCGCTCACGTCGTAGGTACTTTGCAGGTGCTGCCCCCCGAGGAGCG
CGCCCTCATCAAAGGCATCGTGATCAACAAGTTTCGCGGTTCGCTTGCACTGTTGCAGCCGGGCCTCGACTGGCTTGCCC
AGTACACCGGCGTGCCGGTGGTGGGTGTGCTGCCCTGGCTGGAGATGGCCCTACCCGAGGAAGATTCGATGGGTTTATTC
GATCGGCGCGGAGCGCGCAAACAGGCGCAATTGGAGATCGTCGTGATCCGACTGCCGCGCATCGCCAATTTCACGGATTT
CGACGCCCTGGAAGCAGAACCCAGCGTCCGGGTGCGCTACGTGAGCCCCGACGGATACCTTGGTCATCCAGATGCAATTA
TCCTGCCAGGATCAAAAGCCACCATCCCCGACCTGCTTGCCCTCGAAGCGAGCGGCATGGCTGCACAAATCCGGGCTTAC
GGGGGGGTGATCCTAGGAATCTGTGGTGGTCTGCAGATACTGGGAAGCACCATCGACGATCCTGAAGGATTTGAGGGGCA
CCCCGGCCGCCATCCCGGTCTTGGGCTCATCGAGGCTACAACCGTTTTCGAGCCGCTCAAGATTACCCGTCAGGTGCAGG
TCGAATCGCGCCTGCCGGCTGGGGAGCCTGTCGTCGGTTACGAAATTCATCAGGGGCAGACGACTTTTGCTCCCACCCTC
GAAGCGCTGTTTGCCGAGCCCCATCTCGGCGTGGTCAGCAGTAATCGCCGCGTCTGGGGTACCTATTTGCACGGCCTGTT
GGACAACCACGCCTGGCGTCGCCACTGGCTCAACGCGCTTCGGGAGCGCCGGGGCCTGCCACAGCTCCCCGTGCAAAGCG
GCCATTACCGTGAACAGCGCGAGGAGATGTTCGAGCGTCTCGCCGACGCCTGGGAGCCCCATCTGCCGATCGATGGGTTT
GCCCAGTTGGCCGGGTTTGCCTCCCAGGTGTAA

Upstream 100 bases:

>100_bases
TTTGGTAATTTATCAATGGCTCACTGTCAGACTGCTTGAACGTTCCATGCAGCCATTAATATCTTGACCCACAACACCTA
CCTCGCATAGGACAAACACA

Downstream 100 bases:

>100_bases
CAAGCGATTCATGTTCCCATCTATTCTGTTTGCATATTGTTGTTGTTGTGGGCAGCGAGAAAAGAGAAAAACTTCTCAGG
AATATTTTGACAAAAATATA

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 490; Mature: 489

Protein sequence:

>490_residues
MAALMVVGTSSHAGKSLLVTALGRLFHRRGVKVAPFKGQNMALNAYVTAEGHEIGHAQAVQAWACGLEPSVAMNPILLKP
QGNMTSQVILKGKPAGVCGAVDYYRDYFEPGWQAVVEALAELQSQYELVICEGAGSPAEVNLRHRDLTNMRVALHLGAPT
LLVTDIDRGGALAHVVGTLQVLPPEERALIKGIVINKFRGSLALLQPGLDWLAQYTGVPVVGVLPWLEMALPEEDSMGLF
DRRGARKQAQLEIVVIRLPRIANFTDFDALEAEPSVRVRYVSPDGYLGHPDAIILPGSKATIPDLLALEASGMAAQIRAY
GGVILGICGGLQILGSTIDDPEGFEGHPGRHPGLGLIEATTVFEPLKITRQVQVESRLPAGEPVVGYEIHQGQTTFAPTL
EALFAEPHLGVVSSNRRVWGTYLHGLLDNHAWRRHWLNALRERRGLPQLPVQSGHYREQREEMFERLADAWEPHLPIDGF
AQLAGFASQV

Sequences:

>Translated_490_residues
MAALMVVGTSSHAGKSLLVTALGRLFHRRGVKVAPFKGQNMALNAYVTAEGHEIGHAQAVQAWACGLEPSVAMNPILLKP
QGNMTSQVILKGKPAGVCGAVDYYRDYFEPGWQAVVEALAELQSQYELVICEGAGSPAEVNLRHRDLTNMRVALHLGAPT
LLVTDIDRGGALAHVVGTLQVLPPEERALIKGIVINKFRGSLALLQPGLDWLAQYTGVPVVGVLPWLEMALPEEDSMGLF
DRRGARKQAQLEIVVIRLPRIANFTDFDALEAEPSVRVRYVSPDGYLGHPDAIILPGSKATIPDLLALEASGMAAQIRAY
GGVILGICGGLQILGSTIDDPEGFEGHPGRHPGLGLIEATTVFEPLKITRQVQVESRLPAGEPVVGYEIHQGQTTFAPTL
EALFAEPHLGVVSSNRRVWGTYLHGLLDNHAWRRHWLNALRERRGLPQLPVQSGHYREQREEMFERLADAWEPHLPIDGF
AQLAGFASQV
>Mature_489_residues
AALMVVGTSSHAGKSLLVTALGRLFHRRGVKVAPFKGQNMALNAYVTAEGHEIGHAQAVQAWACGLEPSVAMNPILLKPQ
GNMTSQVILKGKPAGVCGAVDYYRDYFEPGWQAVVEALAELQSQYELVICEGAGSPAEVNLRHRDLTNMRVALHLGAPTL
LVTDIDRGGALAHVVGTLQVLPPEERALIKGIVINKFRGSLALLQPGLDWLAQYTGVPVVGVLPWLEMALPEEDSMGLFD
RRGARKQAQLEIVVIRLPRIANFTDFDALEAEPSVRVRYVSPDGYLGHPDAIILPGSKATIPDLLALEASGMAAQIRAYG
GVILGICGGLQILGSTIDDPEGFEGHPGRHPGLGLIEATTVFEPLKITRQVQVESRLPAGEPVVGYEIHQGQTTFAPTLE
ALFAEPHLGVVSSNRRVWGTYLHGLLDNHAWRRHWLNALRERRGLPQLPVQSGHYREQREEMFERLADAWEPHLPIDGFA
QLAGFASQV

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_GLOVI (Q7NJR0)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_924718.1
- ProteinModelPortal:   Q7NJR0
- SMR:   Q7NJR0
- GeneID:   2600174
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.1772
- HOGENOM:   HBG302271
- OMA:   RGDIANM
- ProtClustDB:   PRK00784
- BioCyc:   GVIO251221:GLR1772-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 53243; Mature: 53112

Theoretical pI: Translated: 6.46; Mature: 6.46

Prosite motif: PS51274 GATASE_COBBQ; PS00687 ALDEHYDE_DEHYDR_GLU ; PS00142 ZINC_PROTEASE

Important sites: ACT_SITE 328-328 ACT_SITE 424-424

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAALMVVGTSSHAGKSLLVTALGRLFHRRGVKVAPFKGQNMALNAYVTAEGHEIGHAQAV
CEEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEEECCCCCCCHHHH
QAWACGLEPSVAMNPILLKPQGNMTSQVILKGKPAGVCGAVDYYRDYFEPGWQAVVEALA
HHHHCCCCCCCCCCCEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHH
ELQSQYELVICEGAGSPAEVNLRHRDLTNMRVALHLGAPTLLVTDIDRGGALAHVVGTLQ
HHHCCEEEEEECCCCCCCEEEEEECCCCCEEEEEEECCCEEEEEECCCCCCHHEEHHHHE
VLPPEERALIKGIVINKFRGSLALLQPGLDWLAQYTGVPVVGVLPWLEMALPEEDSMGLF
ECCCHHHHHHHHHHHECCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCH
DRRGARKQAQLEIVVIRLPRIANFTDFDALEAEPSVRVRYVSPDGYLGHPDAIILPGSKA
HHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCC
TIPDLLALEASGMAAQIRAYGGVILGICGGLQILGSTIDDPEGFEGHPGRHPGLGLIEAT
CCCHHHEECCCCCCEEHHHHCCEEEEHHCCHHHHCCCCCCCCCCCCCCCCCCCCCEEEHH
TVFEPLKITRQVQVESRLPAGEPVVGYEIHQGQTTFAPTLEALFAEPHLGVVSSNRRVWG
HHHHHHHHHHEEEHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEECCCEEHH
TYLHGLLDNHAWRRHWLNALRERRGLPQLPVQSGHYREQREEMFERLADAWEPHLPIDGF
HHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
AQLAGFASQV
HHHHHHHHCC
>Mature Secondary Structure 
AALMVVGTSSHAGKSLLVTALGRLFHRRGVKVAPFKGQNMALNAYVTAEGHEIGHAQAV
EEEEEEECCCCCCHHHHHHHHHHHHHHCCCEEECCCCCCEEEEEEEEECCCCCCCHHHH
QAWACGLEPSVAMNPILLKPQGNMTSQVILKGKPAGVCGAVDYYRDYFEPGWQAVVEALA
HHHHCCCCCCCCCCCEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCHHHHHHHHH
ELQSQYELVICEGAGSPAEVNLRHRDLTNMRVALHLGAPTLLVTDIDRGGALAHVVGTLQ
HHHCCEEEEEECCCCCCCEEEEEECCCCCEEEEEEECCCEEEEEECCCCCCHHEEHHHHE
VLPPEERALIKGIVINKFRGSLALLQPGLDWLAQYTGVPVVGVLPWLEMALPEEDSMGLF
ECCCHHHHHHHHHHHECCCCCEEEECCCHHHHHHHCCCCHHHHHHHHHHHCCCCCCCCCH
DRRGARKQAQLEIVVIRLPRIANFTDFDALEAEPSVRVRYVSPDGYLGHPDAIILPGSKA
HHCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEECCCCC
TIPDLLALEASGMAAQIRAYGGVILGICGGLQILGSTIDDPEGFEGHPGRHPGLGLIEAT
CCCHHHEECCCCCCEEHHHHCCEEEEHHCCHHHHCCCCCCCCCCCCCCCCCCCCCEEEHH
TVFEPLKITRQVQVESRLPAGEPVVGYEIHQGQTTFAPTLEALFAEPHLGVVSSNRRVWG
HHHHHHHHHHEEEHHHCCCCCCCEEEEEEECCCCCHHHHHHHHHCCCCCCEEECCCEEHH
TYLHGLLDNHAWRRHWLNALRERRGLPQLPVQSGHYREQREEMFERLADAWEPHLPIDGF
HHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHH
AQLAGFASQV
HHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292