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The map label for this gene is yafV [C]

Identifier: 37521251

GI number: 37521251

Start: 1793944

End: 1794798

Strand: Direct

Name: yafV [C]

Synonym: glr1682

Alternate gene names: 37521251

Gene position: 1793944-1794798 (Clockwise)

Preceding gene: 37521250

Following gene: 37521253

Centisome position: 38.5

GC content: 65.5

Gene sequence:

>855_bases
ATGAGTGCTCTCACCGTCGCCGCTCTGCAAACAGCCTTTGTCGAAGATGTGGCGACCAACGTCGAGCGCGTCAGCGCCCT
GGTGCGCGAGGCCGCCGAGCGGGGAGCGCAGATTATTTTGCCCTCGGAGCTGTTCGAGAGCCACTATTTTTGCCGGGTGG
AACGCGACCAGTTTTTTGATCTGGCCCGACCGGCCGAGGGGCACCCGACTATCGCCCACTTCCAGGCCATCGCCGCCGAA
CTGGGGGTGGTGATCCCCGTCTCGTTTTTCGAGCGCGCCGGCCAGAGCCACTACAACAGCATCGCCATCGTCGATGCCGA
CGGCCGCTGCCTGGGCACCTACCGCAAAAGCCACATCCCCGACGGCCCCGGCTACGAAGAAAAATTCTACTTCCGCCCCG
GCAACACCGGCTTTCGGGTCTGGCGCACCCGCTACGCCACCATCGGCGTCGGCATCTGCTGGGATCAGTGGTTTCCGGAG
GCGGCCCGGGCGATGGTGCTGATGGGGGCGGAGGTGCTCTTCTATCCGACCGCCATCGGCAGCGAACCCGAGAGCCCGGA
TCTCGACACCAAAGACCCCTGGCAGCGGGCGATGGTAGGCCACGCCGTCTCCAACGTCATCCCGGTGGTGGCGGCCAACC
GCACCGGCACCGAGGGCAATCAGACTTTCTACGGCCACTCGTTTATCTGCAACCACCGCGGCGATACAGTGGCCGAGTTG
GGCCGCAAAGAAGAAGGGGTGATCACCGCCAGCTTCGACCTGGCGGAGGTGCGCCGCCACCGCGCTTCGTTCGGGTTTTT
CCGCGACCGGCGTCCGGAGCTGTACGGCCTGCTCACCACCGCCGACGGCCAGTAG

Upstream 100 bases:

>100_bases
TCTTCCCGGGCCGCCGGACGGTCGGTCTGCCGGCGCGCACCATCTTGCTGGGGGGCGGCGCGTTCCACTGCATCACCCAA
CAACAACCGGAGGCCGGATC

Downstream 100 bases:

>100_bases
GCGCTGCAGGAGAGAGCCCGCCGCTCCCTGCCCTGGGATGCGTCCGCAAAGAAACGCTTCTTCCTACCGGTTGAGTGTTA
CTTTCTATCTCATGACTGAG

Product: hydratase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSALTVAALQTAFVEDVATNVERVSALVREAAERGAQIILPSELFESHYFCRVERDQFFDLARPAEGHPTIAHFQAIAAE
LGVVIPVSFFERAGQSHYNSIAIVDADGRCLGTYRKSHIPDGPGYEEKFYFRPGNTGFRVWRTRYATIGVGICWDQWFPE
AARAMVLMGAEVLFYPTAIGSEPESPDLDTKDPWQRAMVGHAVSNVIPVVAANRTGTEGNQTFYGHSFICNHRGDTVAEL
GRKEEGVITASFDLAEVRRHRASFGFFRDRRPELYGLLTTADGQ

Sequences:

>Translated_284_residues
MSALTVAALQTAFVEDVATNVERVSALVREAAERGAQIILPSELFESHYFCRVERDQFFDLARPAEGHPTIAHFQAIAAE
LGVVIPVSFFERAGQSHYNSIAIVDADGRCLGTYRKSHIPDGPGYEEKFYFRPGNTGFRVWRTRYATIGVGICWDQWFPE
AARAMVLMGAEVLFYPTAIGSEPESPDLDTKDPWQRAMVGHAVSNVIPVVAANRTGTEGNQTFYGHSFICNHRGDTVAEL
GRKEEGVITASFDLAEVRRHRASFGFFRDRRPELYGLLTTADGQ
>Mature_283_residues
SALTVAALQTAFVEDVATNVERVSALVREAAERGAQIILPSELFESHYFCRVERDQFFDLARPAEGHPTIAHFQAIAAEL
GVVIPVSFFERAGQSHYNSIAIVDADGRCLGTYRKSHIPDGPGYEEKFYFRPGNTGFRVWRTRYATIGVGICWDQWFPEA
ARAMVLMGAEVLFYPTAIGSEPESPDLDTKDPWQRAMVGHAVSNVIPVVAANRTGTEGNQTFYGHSFICNHRGDTVAELG
RKEEGVITASFDLAEVRRHRASFGFFRDRRPELYGLLTTADGQ

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI297632348, Length=286, Percent_Identity=29.7202797202797, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI9910460, Length=286, Percent_Identity=29.020979020979, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI297632350, Length=286, Percent_Identity=29.7202797202797, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI5031947, Length=286, Percent_Identity=29.7202797202797, Blast_Score=105, Evalue=5e-23,
Organism=Homo sapiens, GI7706509, Length=283, Percent_Identity=28.2685512367491, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI297632346, Length=188, Percent_Identity=30.3191489361702, Blast_Score=75, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17533173, Length=279, Percent_Identity=28.673835125448, Blast_Score=109, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17556280, Length=283, Percent_Identity=24.0282685512367, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6323383, Length=272, Percent_Identity=24.6323529411765, Blast_Score=87, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21358471, Length=302, Percent_Identity=29.1390728476821, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17933642, Length=279, Percent_Identity=26.5232974910394, Blast_Score=80, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 31552; Mature: 31421

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSALTVAALQTAFVEDVATNVERVSALVREAAERGAQIILPSELFESHYFCRVERDQFFD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCCCCEEEECHHHHHH
LARPAEGHPTIAHFQAIAAELGVVIPVSFFERAGQSHYNSIAIVDADGRCLGTYRKSHIP
CCCCCCCCCHHHHHHHHHHHCCEEEEHHHHHHCCCCCCCEEEEECCCCCEEECHHHCCCC
DGPGYEEKFYFRPGNTGFRVWRTRYATIGVGICWDQWFPEAARAMVLMGAEVLFYPTAIG
CCCCCCCEEEECCCCCCEEEEEEEEEEEEEEEHHHHHCHHHHHHHHHHCCEEEEEECCCC
SEPESPDLDTKDPWQRAMVGHAVSNVIPVVAANRTGTEGNQTFYGHSFICNHRGDTVAEL
CCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEECCEEEECCCCCHHHHH
GRKEEGVITASFDLAEVRRHRASFGFFRDRRPELYGLLTTADGQ
CCCCCCEEEEECCHHHHHHHHHHHCHHHCCCCCEEEEEEECCCC
>Mature Secondary Structure 
SALTVAALQTAFVEDVATNVERVSALVREAAERGAQIILPSELFESHYFCRVERDQFFD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHCCCCEEEECHHHHHH
LARPAEGHPTIAHFQAIAAELGVVIPVSFFERAGQSHYNSIAIVDADGRCLGTYRKSHIP
CCCCCCCCCHHHHHHHHHHHCCEEEEHHHHHHCCCCCCCEEEEECCCCCEEECHHHCCCC
DGPGYEEKFYFRPGNTGFRVWRTRYATIGVGICWDQWFPEAARAMVLMGAEVLFYPTAIG
CCCCCCCEEEECCCCCCEEEEEEEEEEEEEEEHHHHHCHHHHHHHHHHCCEEEEEECCCC
SEPESPDLDTKDPWQRAMVGHAVSNVIPVVAANRTGTEGNQTFYGHSFICNHRGDTVAEL
CCCCCCCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEECCEEEECCCCCHHHHH
GRKEEGVITASFDLAEVRRHRASFGFFRDRRPELYGLLTTADGQ
CCCCCCEEEEECCHHHHHHHHHHHCHHHCCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]