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The map label for this gene is dnaK [H]

Identifier: 37521245

GI number: 37521245

Start: 1789709

End: 1791286

Strand: Reverse

Name: dnaK [H]

Synonym: gvip233

Alternate gene names: 37521245

Gene position: 1791286-1789709 (Counterclockwise)

Preceding gene: 37521248

Following gene: 37521243

Centisome position: 38.45

GC content: 65.15

Gene sequence:

>1578_bases
GTGTACAGTTTCGACTTTGGGACCAGCAACACCGTCGTGGCCCGCTGGAATCGCGCCCTCGCCCAGCCGGAGACCCTTGC
CGTCAGGGGCCTGAGCCAGGTGGAATCACCGCCGCTCGTGCCAAGCGTGCTCTATGTGCAGGATGCGGGAGCGGACCGGG
TGCTGGCGGGCCAGGAGGTGCTCGCCCGCGGCCTGGATAACAGCGGCGATCCGCGTTTTTTCAGCAACTTCAAACGGGGG
ATCGGCTCGGCGGTGCAAGGATTCGTCCCCGCCCTCGACGGTGTCGAAGTCGGTTTTGAACGGGTGGGGCTGTGGTTCAT
CCGGCGATTGATCGCCGCGCTGAGAGATCAAGGCGAATCACCCGACGACGTCGTGTTCACCGTCCCGGTCAACAGCTTCG
AGTTGTACCGCCAGTGGCTATTGGCCGGATGCAGCGAACTAGAGGCGACGCGCATCCAGTTGCTCGACGAATCGACCGCC
GCCGCCCTGGGCTATGGGCTCGAAAATGGGCAGACGGTGCTGGTCATCGACTTTGGCGGCGGTACGCTGGATCTGTCGCT
GGTGCAACCGGCGGTACCCGAGCAGAGTCCGGACGGCTTCTTGCTCAAGTGGGGCCGCAAGCTCTTTGCCGGCAAAGACA
GCGCGCCGCGCACCCCCACCGCCCGGGTACTCGCCAAGGTCGGGCGCAACCTGGGAGGCATGGACATCGACACCTGGCTT
GCCGACAGCCTCGCCCGCCAACAGAAACTTCCCAAAGGGGCGCTGTTGCAGCGGGTCGCCGAGCGGCTCAAGATCCGGCT
TTCGAGCGAAACGGCGGCCACGGAGGTCTATTTTGACGAAGATTCGCTGCGCACCTGCAAGCTGACACTGGAGCGCGAAC
AGTTCGAAGCGCTCCTGGCCGCGCGCGGCTTTATCGCCCAACTCGACGAGAGCCTGGGCAAGCTGTTGCAGCAGGCCCGC
CAGCGCAACCTCGATCCGGAGGCGATCGACGCGGTGATTCTGGTGGGAGGTACCTGTCAGATCCCGGCGGTGCAAAACTG
GGTGGTCGATCGCTTCGGGGCCGCCAAAGTGCGCAAGGACAAAGTGTTCGAAGCGGTGGCCCATGGGGCGCTGCGCCTGG
GCCAGGGGTTGCAGGTCGAAGATTTTCTCTACCACGCCTACGGAGTGCGCTACTGGGATCACCGTAAATCTCGCCACGGC
TGGCACGCGCTGTTTAAGGCCGGACAGGCCTACCCCACCGCCAACCCCGTCGAACTGGTGCTGGGGGCCTCGGTCTCCAA
CCAGCCGGTCATCGAACTGGTGGTGGGCGAAATGGGCGACGGAGAAGACACCGAGGTCTTCTTCGAGGACGGTCGGCTTA
CCATCCGCACGAGCGCGTCCGAGCAGACCGTCCGGTCCCTCAACGACACGGACGAAGGCCGGGTGGTGGCCCGGCTCGAA
CCGCCCGGCTTTCCGGGGCGCGACCGGGTGAAGGTGCAACTGCGAGTCGACGCCCGGCGCCAGCTGCGCATCACGGTGGA
GGACTTGCAGACCCAGCAGACACTCCTGCAAGATCAGCCGGTCGTCGAGTTGCGCTAG

Upstream 100 bases:

>100_bases
CCTCCAGGTACGACCGAGGATACGCACGAAGGCACACGATGCCTCCGCACAAAAGCGGCGGTAAGATTCGATTCAGTGGG
TATCTATTGAACAACGCGTA

Downstream 100 bases:

>100_bases
GAGCCGCCGGAGTCTCGCTTTGGCGCTATCGTGCCAATTTCTATGAGCACGATTCTGAAAAAAGATCTCCAAAGACTCAA
ACACAGGTCGGAAGCCTGGT

Product: DnaK-type molecular chaperone

Products: NA

Alternate protein names: HSP70; Heat shock 70 kDa protein; Heat shock protein 70 [H]

Number of amino acids: Translated: 525; Mature: 525

Protein sequence:

>525_residues
MYSFDFGTSNTVVARWNRALAQPETLAVRGLSQVESPPLVPSVLYVQDAGADRVLAGQEVLARGLDNSGDPRFFSNFKRG
IGSAVQGFVPALDGVEVGFERVGLWFIRRLIAALRDQGESPDDVVFTVPVNSFELYRQWLLAGCSELEATRIQLLDESTA
AALGYGLENGQTVLVIDFGGGTLDLSLVQPAVPEQSPDGFLLKWGRKLFAGKDSAPRTPTARVLAKVGRNLGGMDIDTWL
ADSLARQQKLPKGALLQRVAERLKIRLSSETAATEVYFDEDSLRTCKLTLEREQFEALLAARGFIAQLDESLGKLLQQAR
QRNLDPEAIDAVILVGGTCQIPAVQNWVVDRFGAAKVRKDKVFEAVAHGALRLGQGLQVEDFLYHAYGVRYWDHRKSRHG
WHALFKAGQAYPTANPVELVLGASVSNQPVIELVVGEMGDGEDTEVFFEDGRLTIRTSASEQTVRSLNDTDEGRVVARLE
PPGFPGRDRVKVQLRVDARRQLRITVEDLQTQQTLLQDQPVVELR

Sequences:

>Translated_525_residues
MYSFDFGTSNTVVARWNRALAQPETLAVRGLSQVESPPLVPSVLYVQDAGADRVLAGQEVLARGLDNSGDPRFFSNFKRG
IGSAVQGFVPALDGVEVGFERVGLWFIRRLIAALRDQGESPDDVVFTVPVNSFELYRQWLLAGCSELEATRIQLLDESTA
AALGYGLENGQTVLVIDFGGGTLDLSLVQPAVPEQSPDGFLLKWGRKLFAGKDSAPRTPTARVLAKVGRNLGGMDIDTWL
ADSLARQQKLPKGALLQRVAERLKIRLSSETAATEVYFDEDSLRTCKLTLEREQFEALLAARGFIAQLDESLGKLLQQAR
QRNLDPEAIDAVILVGGTCQIPAVQNWVVDRFGAAKVRKDKVFEAVAHGALRLGQGLQVEDFLYHAYGVRYWDHRKSRHG
WHALFKAGQAYPTANPVELVLGASVSNQPVIELVVGEMGDGEDTEVFFEDGRLTIRTSASEQTVRSLNDTDEGRVVARLE
PPGFPGRDRVKVQLRVDARRQLRITVEDLQTQQTLLQDQPVVELR
>Mature_525_residues
MYSFDFGTSNTVVARWNRALAQPETLAVRGLSQVESPPLVPSVLYVQDAGADRVLAGQEVLARGLDNSGDPRFFSNFKRG
IGSAVQGFVPALDGVEVGFERVGLWFIRRLIAALRDQGESPDDVVFTVPVNSFELYRQWLLAGCSELEATRIQLLDESTA
AALGYGLENGQTVLVIDFGGGTLDLSLVQPAVPEQSPDGFLLKWGRKLFAGKDSAPRTPTARVLAKVGRNLGGMDIDTWL
ADSLARQQKLPKGALLQRVAERLKIRLSSETAATEVYFDEDSLRTCKLTLEREQFEALLAARGFIAQLDESLGKLLQQAR
QRNLDPEAIDAVILVGGTCQIPAVQNWVVDRFGAAKVRKDKVFEAVAHGALRLGQGLQVEDFLYHAYGVRYWDHRKSRHG
WHALFKAGQAYPTANPVELVLGASVSNQPVIELVVGEMGDGEDTEVFFEDGRLTIRTSASEQTVRSLNDTDEGRVVARLE
PPGFPGRDRVKVQLRVDARRQLRITVEDLQTQQTLLQDQPVVELR

Specific function: Acts as a chaperone [H]

COG id: COG0443

COG function: function code O; Molecular chaperone

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the heat shock protein 70 family [H]

Homologues:

Organism=Homo sapiens, GI24234688, Length=269, Percent_Identity=30.1115241635688, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786870, Length=388, Percent_Identity=26.2886597938144, Blast_Score=70, Evalue=4e-13,
Organism=Escherichia coli, GI1788875, Length=388, Percent_Identity=28.0927835051546, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17534015, Length=269, Percent_Identity=29.7397769516729, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17534013, Length=269, Percent_Identity=29.7397769516729, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507981, Length=274, Percent_Identity=30.2919708029197, Blast_Score=72, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324120, Length=274, Percent_Identity=32.1167883211679, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319972, Length=274, Percent_Identity=32.1167883211679, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24646137, Length=271, Percent_Identity=31.3653136531365, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646135, Length=271, Percent_Identity=31.3653136531365, Blast_Score=74, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646324, Length=271, Percent_Identity=30.6273062730627, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24646322, Length=271, Percent_Identity=30.6273062730627, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI28571678, Length=271, Percent_Identity=30.6273062730627, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24646318, Length=271, Percent_Identity=30.6273062730627, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: 1300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 320 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 500 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stati

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012725
- InterPro:   IPR018181
- InterPro:   IPR001023
- InterPro:   IPR013126 [H]

Pfam domain/function: PF00012 HSP70 [H]

EC number: NA

Molecular weight: Translated: 57874; Mature: 57874

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00329 HSP70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYSFDFGTSNTVVARWNRALAQPETLAVRGLSQVESPPLVPSVLYVQDAGADRVLAGQEV
CCEECCCCCCCEEEEHHHHHCCCHHHHHHCHHHCCCCCCCCEEEEEECCCCCCCHHHHHH
LARGLDNSGDPRFFSNFKRGIGSAVQGFVPALDGVEVGFERVGLWFIRRLIAALRDQGES
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
PDDVVFTVPVNSFELYRQWLLAGCSELEATRIQLLDESTAAALGYGLENGQTVLVIDFGG
CCCEEEEEECCHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCC
GTLDLSLVQPAVPEQSPDGFLLKWGRKLFAGKDSAPRTPTARVLAKVGRNLGGMDIDTWL
CEEEEEEECCCCCCCCCCCHHHHHCHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHH
ADSLARQQKLPKGALLQRVAERLKIRLSSETAATEVYFDEDSLRTCKLTLEREQFEALLA
HHHHHHHHCCCHHHHHHHHHHHHEEEECCCCCEEEEEECCCCCEEEEEEECHHHHHHHHH
ARGFIAQLDESLGKLLQQARQRNLDPEAIDAVILVGGTCQIPAVQNWVVDRFGAAKVRKD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHCEEEEECCCCCCCCHHHHHHHHCCCHHHHHH
KVFEAVAHGALRLGQGLQVEDFLYHAYGVRYWDHRKSRHGWHALFKAGQAYPTANPVELV
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHCCCCCCHHHHHHHCCCCCCCCCCEEEE
LGASVSNQPVIELVVGEMGDGEDTEVFFEDGRLTIRTSASEQTVRSLNDTDEGRVVARLE
EECCCCCCCEEEEEECCCCCCCCCEEEEECCEEEEEECCHHHHHHHCCCCCCCEEEEEEC
PPGFPGRDRVKVQLRVDARRQLRITVEDLQTQQTLLQDQPVVELR
CCCCCCCCEEEEEEEECCCCEEEEEHHHHHHHHHHHCCCCCEECC
>Mature Secondary Structure
MYSFDFGTSNTVVARWNRALAQPETLAVRGLSQVESPPLVPSVLYVQDAGADRVLAGQEV
CCEECCCCCCCEEEEHHHHHCCCHHHHHHCHHHCCCCCCCCEEEEEECCCCCCCHHHHHH
LARGLDNSGDPRFFSNFKRGIGSAVQGFVPALDGVEVGFERVGLWFIRRLIAALRDQGES
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
PDDVVFTVPVNSFELYRQWLLAGCSELEATRIQLLDESTAAALGYGLENGQTVLVIDFGG
CCCEEEEEECCHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHHHCCCCCCCEEEEEECCC
GTLDLSLVQPAVPEQSPDGFLLKWGRKLFAGKDSAPRTPTARVLAKVGRNLGGMDIDTWL
CEEEEEEECCCCCCCCCCCHHHHHCHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHH
ADSLARQQKLPKGALLQRVAERLKIRLSSETAATEVYFDEDSLRTCKLTLEREQFEALLA
HHHHHHHHCCCHHHHHHHHHHHHEEEECCCCCEEEEEECCCCCEEEEEEECHHHHHHHHH
ARGFIAQLDESLGKLLQQARQRNLDPEAIDAVILVGGTCQIPAVQNWVVDRFGAAKVRKD
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHCEEEEECCCCCCCCHHHHHHHHCCCHHHHHH
KVFEAVAHGALRLGQGLQVEDFLYHAYGVRYWDHRKSRHGWHALFKAGQAYPTANPVELV
HHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHCCCCCCHHHHHHHCCCCCCCCCCEEEE
LGASVSNQPVIELVVGEMGDGEDTEVFFEDGRLTIRTSASEQTVRSLNDTDEGRVVARLE
EECCCCCCCEEEEEECCCCCCCCCEEEEECCEEEEEECCHHHHHHHCCCCCCCEEEEEEC
PPGFPGRDRVKVQLRVDARRQLRITVEDLQTQQTLLQDQPVVELR
CCCCCCCCEEEEEEEECCCCEEEEEHHHHHHHHHHHCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA