Click here to switch to the map view.

The map label for this gene is pyrF

Identifier: 37521227

GI number: 37521227

Start: 1772385

End: 1773098

Strand: Reverse

Name: pyrF

Synonym: gvip232

Alternate gene names: 37521227

Gene position: 1773098-1772385 (Counterclockwise)

Preceding gene: 37521228

Following gene: 37521226

Centisome position: 38.06

GC content: 70.03

Gene sequence:

>714_bases
ATGGGTGGTGCCGAACGGGTGATCGTGGCCCTCGATCTCGCGGACGCCCAGGCGGCCCGCGCCCTGGTGGAGCGCGCCCC
GGCCGTGCGCTTCTGGAAAGTCGGACTGGAACTATTCACCGCCGCCGGTCCGGGCCTGATCGAATGGCTCAAAGCGCGCG
GGTGCCGGGTGTTTCTCGATCTCAAATTTCACGATATTCCCAACACGGTGGCCGGGGCCTGCCGGGCGGCCACCCGCCTG
GGGGTGGACCTGCTCACCGTGCATGCGACGGGGGGACAACCGATGCTGATGGCGGCGGTCTCCGCCTGCACAGGCGAAGC
GGCCCGCCTCGGGAGCCCCGCCCCGGCGGTGCTGGCGGTGACCCTGCTCACCAGCCTGGACGCGCAGCTGCTCGAACAGC
AGCTCTTGGTCAATGTCGGGGTAAGCGGCTATGTGGAGCACCTCGCGGTCCTGGCCGTGTCAAGCGGTGTGCAGGGGGTG
GTCTGCTCGCCTCTGGAGACGGCCCTGCTGCGCGAGCGCTGCGGAAACGATTTTCTGATCGTCACCCCGGGCATTCGCCC
GGGTGGGAGCGGCGAAGGCGACCAGCGCCGCACCCTAACCCCCGGCGAAGCGTTTGCCCGGGGCGCCGACTACCTGGTGG
TGGGCCGCCCGGTGACGACCGCCCCCGATCCCCAGGTCGCATTCAAGGCCATCGTCGCGGAGGTCGGCGGATGA

Upstream 100 bases:

>100_bases
CGCGTCAGCAACTGGATGTAGCGGAGTTGCAGACTTTCTTGCGGCATCTGAAGGCCCAACCCAACCACCTCGCCCGCCGC
AGCACCCAGGCGCCGTTTTG

Downstream 100 bases:

>100_bases
TGCGTACTTTGTTGCCGGCCCTTGCTCTGGCTACCTGGCTGGCCCTCGCACCTGTGCCGACTCCCGCCGCTCCGGCCATC
GAAGCCGAGCGGCTCGCAGA

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 237; Mature: 236

Protein sequence:

>237_residues
MGGAERVIVALDLADAQAARALVERAPAVRFWKVGLELFTAAGPGLIEWLKARGCRVFLDLKFHDIPNTVAGACRAATRL
GVDLLTVHATGGQPMLMAAVSACTGEAARLGSPAPAVLAVTLLTSLDAQLLEQQLLVNVGVSGYVEHLAVLAVSSGVQGV
VCSPLETALLRERCGNDFLIVTPGIRPGGSGEGDQRRTLTPGEAFARGADYLVVGRPVTTAPDPQVAFKAIVAEVGG

Sequences:

>Translated_237_residues
MGGAERVIVALDLADAQAARALVERAPAVRFWKVGLELFTAAGPGLIEWLKARGCRVFLDLKFHDIPNTVAGACRAATRL
GVDLLTVHATGGQPMLMAAVSACTGEAARLGSPAPAVLAVTLLTSLDAQLLEQQLLVNVGVSGYVEHLAVLAVSSGVQGV
VCSPLETALLRERCGNDFLIVTPGIRPGGSGEGDQRRTLTPGEAFARGADYLVVGRPVTTAPDPQVAFKAIVAEVGG
>Mature_236_residues
GGAERVIVALDLADAQAARALVERAPAVRFWKVGLELFTAAGPGLIEWLKARGCRVFLDLKFHDIPNTVAGACRAATRLG
VDLLTVHATGGQPMLMAAVSACTGEAARLGSPAPAVLAVTLLTSLDAQLLEQQLLVNVGVSGYVEHLAVLAVSSGVQGVV
CSPLETALLRERCGNDFLIVTPGIRPGGSGEGDQRRTLTPGEAFARGADYLVVGRPVTTAPDPQVAFKAIVAEVGG

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=234, Percent_Identity=45.7264957264957, Blast_Score=175, Evalue=3e-45,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_GLOVI (Q7NK22)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_924604.1
- ProteinModelPortal:   Q7NK22
- SMR:   Q7NK22
- GeneID:   2598438
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.1658
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- BioCyc:   GVIO251221:GLL1658-MONOMER
- BRENDA:   4.1.1.23
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 24471; Mature: 24339

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 62-62 BINDING 12-12 BINDING 33-33 BINDING 124-124 BINDING 186-186 BINDING 195-195 BINDING 215-215 BINDING 216-216

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGAERVIVALDLADAQAARALVERAPAVRFWKVGLELFTAAGPGLIEWLKARGCRVFLD
CCCCCEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEE
LKFHDIPNTVAGACRAATRLGVDLLTVHATGGQPMLMAAVSACTGEAARLGSPAPAVLAV
EEECCCCHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHCCCHHHCCCCCHHHHHH
TLLTSLDAQLLEQQLLVNVGVSGYVEHLAVLAVSSGVQGVVCSPLETALLRERCGNDFLI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHCCCCEEE
VTPGIRPGGSGEGDQRRTLTPGEAFARGADYLVVGRPVTTAPDPQVAFKAIVAEVGG
EECCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
GGAERVIVALDLADAQAARALVERAPAVRFWKVGLELFTAAGPGLIEWLKARGCRVFLD
CCCCEEEEEEECCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCCEEEEE
LKFHDIPNTVAGACRAATRLGVDLLTVHATGGQPMLMAAVSACTGEAARLGSPAPAVLAV
EEECCCCHHHHHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHCCCHHHCCCCCHHHHHH
TLLTSLDAQLLEQQLLVNVGVSGYVEHLAVLAVSSGVQGVVCSPLETALLRERCGNDFLI
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHCCCCEEE
VTPGIRPGGSGEGDQRRTLTPGEAFARGADYLVVGRPVTTAPDPQVAFKAIVAEVGG
EECCCCCCCCCCCCCCCCCCCHHHHHCCCCEEEECCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292