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The map label for this gene is kefA [H]

Identifier: 37521184

GI number: 37521184

Start: 1730442

End: 1731341

Strand: Reverse

Name: kefA [H]

Synonym: gll1615

Alternate gene names: 37521184

Gene position: 1731341-1730442 (Counterclockwise)

Preceding gene: 37521185

Following gene: 37521182

Centisome position: 37.16

GC content: 62.89

Gene sequence:

>900_bases
ATGGAGCAGGTACTGCAATATCTTGGCCGGTTTTTTACCCAGCCGCTGTTTACGATCAGCGGCACGGCCGTCTCGGCCAC
CTCGGTGCTGGTCTTCGCGGTGCTGACCGTGATGTCCATCTGGCTCGGGCGCCAGGGGCGGCGGCTGGTGCGGCAGCTGG
TGGCGGGCCGATTGGAAGCCACCCTCGCCAACGCCCTCGATCAGATCGTCAACGTGCTGGTCATCGTCGCCGGACTTTAC
ATCGCCCTGCGCTTCGTAGGAATCGACGTGAGCGGTCTGGTGGTGCTGGCAAGCGCCCTGGGCGTGGGCATCGGCTTTGG
CTTACAAAATATCGCCTCCAACTTGATTTCAGGAGTGATCATTCTGCTGGAGCGGCCGATTTCGGTGGGCGACCGGGTGA
CCGTCGGAGACACGATCGGCGATGTCAGCCACATCGGCCTGCGCTCGACGGAGATCATCACCCCCGACAACATCATGATC
ATCGTCCCCAACACCGAATTTGTGAGCAACCGTGTCATCAACTGGACGCGCGGCAAGCCCCAGACGCGGGTACGTTTGCC
CATCGGCGTCGCCTACGGCAGCGATCTCGACCGAACCCGGCAGGTATTGCTGGGTGTGGCAGCCCGACAAACAGGAGTAC
TGGCCGAGCCGGCGCCGGAGGTGTGGCTGGCGGGCTTCGGCGATTCGTCGCTCAATCTCGAACTTCTCGTCTGGGTAAGT
GCGCCCAATTTGATCCCAAGACTCAAATCCCAGCTCAACTTCGCCGTCGAGGCGGCCCTGCGCACCCACCGGATTGCGAT
TCCCTTTCCCCAACGCGAGGTGCGCGTCGTCATCGACGGCACCACCAACGGCAGCATCCCCCCGACAGGCAGCCGCAGCC
CGGAGGAGACTTCCCGCTAA

Upstream 100 bases:

>100_bases
GATCGTGCAGGTGGACCCGCGCCAGGATCTGCTGGTACTCAAGGAAGTGCGCCAGGCCCACTGACGGTTACCCTGGGAAA
GATAGCGACGCCTGGAGCCC

Downstream 100 bases:

>100_bases
ACCTCCGGGTTATCGCCCTTGTTGGCCATCTCCTCGGTGATCCAATCCGGCAGTTCGTTCGTTTGGCTGGGCCAGGTGCG
CGCCTGGGTCTGCGGCGGTT

Product: mechanosensitive channel protein

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 299; Mature: 299

Protein sequence:

>299_residues
MEQVLQYLGRFFTQPLFTISGTAVSATSVLVFAVLTVMSIWLGRQGRRLVRQLVAGRLEATLANALDQIVNVLVIVAGLY
IALRFVGIDVSGLVVLASALGVGIGFGLQNIASNLISGVIILLERPISVGDRVTVGDTIGDVSHIGLRSTEIITPDNIMI
IVPNTEFVSNRVINWTRGKPQTRVRLPIGVAYGSDLDRTRQVLLGVAARQTGVLAEPAPEVWLAGFGDSSLNLELLVWVS
APNLIPRLKSQLNFAVEAALRTHRIAIPFPQREVRVVIDGTTNGSIPPTGSRSPEETSR

Sequences:

>Translated_299_residues
MEQVLQYLGRFFTQPLFTISGTAVSATSVLVFAVLTVMSIWLGRQGRRLVRQLVAGRLEATLANALDQIVNVLVIVAGLY
IALRFVGIDVSGLVVLASALGVGIGFGLQNIASNLISGVIILLERPISVGDRVTVGDTIGDVSHIGLRSTEIITPDNIMI
IVPNTEFVSNRVINWTRGKPQTRVRLPIGVAYGSDLDRTRQVLLGVAARQTGVLAEPAPEVWLAGFGDSSLNLELLVWVS
APNLIPRLKSQLNFAVEAALRTHRIAIPFPQREVRVVIDGTTNGSIPPTGSRSPEETSR
>Mature_299_residues
MEQVLQYLGRFFTQPLFTISGTAVSATSVLVFAVLTVMSIWLGRQGRRLVRQLVAGRLEATLANALDQIVNVLVIVAGLY
IALRFVGIDVSGLVVLASALGVGIGFGLQNIASNLISGVIILLERPISVGDRVTVGDTIGDVSHIGLRSTEIITPDNIMI
IVPNTEFVSNRVINWTRGKPQTRVRLPIGVAYGSDLDRTRQVLLGVAARQTGVLAEPAPEVWLAGFGDSSLNLELLVWVS
APNLIPRLKSQLNFAVEAALRTHRIAIPFPQREVRVVIDGTTNGSIPPTGSRSPEETSR

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=271, Percent_Identity=38.7453874538745, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI2367355, Length=221, Percent_Identity=35.7466063348416, Blast_Score=132, Evalue=2e-32,
Organism=Escherichia coli, GI1789291, Length=231, Percent_Identity=29.8701298701299, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1787591, Length=273, Percent_Identity=27.1062271062271, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 32171; Mature: 32171

Theoretical pI: Translated: 10.49; Mature: 10.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQVLQYLGRFFTQPLFTISGTAVSATSVLVFAVLTVMSIWLGRQGRRLVRQLVAGRLEA
CHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
TLANALDQIVNVLVIVAGLYIALRFVGIDVSGLVVLASALGVGIGFGLQNIASNLISGVI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ILLERPISVGDRVTVGDTIGDVSHIGLRSTEIITPDNIMIIVPNTEFVSNRVINWTRGKP
HHHCCCCCCCCEEEECCCCCCHHHCCCCCCEEECCCCEEEEECCCHHHHCCCEEECCCCC
QTRVRLPIGVAYGSDLDRTRQVLLGVAARQTGVLAEPAPEVWLAGFGDSSLNLELLVWVS
CCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEEEEEEE
APNLIPRLKSQLNFAVEAALRTHRIAIPFPQREVRVVIDGTTNGSIPPTGSRSPEETSR
CCCHHHHHHHHHHHHHHHHHHHCEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MEQVLQYLGRFFTQPLFTISGTAVSATSVLVFAVLTVMSIWLGRQGRRLVRQLVAGRLEA
CHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
TLANALDQIVNVLVIVAGLYIALRFVGIDVSGLVVLASALGVGIGFGLQNIASNLISGVI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ILLERPISVGDRVTVGDTIGDVSHIGLRSTEIITPDNIMIIVPNTEFVSNRVINWTRGKP
HHHCCCCCCCCEEEECCCCCCHHHCCCCCCEEECCCCEEEEECCCHHHHCCCEEECCCCC
QTRVRLPIGVAYGSDLDRTRQVLLGVAARQTGVLAEPAPEVWLAGFGDSSLNLELLVWVS
CCEEEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCCCEEEEEEEE
APNLIPRLKSQLNFAVEAALRTHRIAIPFPQREVRVVIDGTTNGSIPPTGSRSPEETSR
CCCHHHHHHHHHHHHHHHHHHHCEEECCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]