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The map label for this gene is nirA [H]

Identifier: 37521126

GI number: 37521126

Start: 1677158

End: 1678714

Strand: Reverse

Name: nirA [H]

Synonym: gvip212

Alternate gene names: 37521126

Gene position: 1678714-1677158 (Counterclockwise)

Preceding gene: 37521130

Following gene: 37521125

Centisome position: 36.03

GC content: 63.07

Gene sequence:

>1557_bases
GTGGAAAGGAGCTGCCGTTATTGTCTGGAAGGTGCTGTGGCAAACCCGATCGAAAAAATCAAGGATGAAAAACCCGGTCT
TGCCGTCAAGGCCGAGCTGATCGAATTTGCCCGTCTCGGGTGGGAGGCGATTTCCAAGGGCGATCTGGAGCGGCTGAAGT
GGATCGGCTGGTTCTTCCGCAAGAAGACGCCGGGCCGCTGGATGGTGCGTCTGCGCGTACCGGGGGGGATCTTGAGCAGC
GAGCAGTTGCGGGTAGTTGGGGGGATTCTTGCGGAATTCTCGGACGTCAAGCAACTGGATATCACCACCCGCCAGAATCT
CGAGATGCGTGGATTGCGCATCGAGGACAGCGTATCTATCCAGGAGCGCCTTGAGGCGGTGGGGTTGACCACGGTGCAAT
CGGGCATGGACAACGTGCGCAACATCGTGGGCTCCCCGGTGGCGGGCATCGATCCTGACGAACTCATCGATGTGCGCCCG
TTGGTGCAGCAAATGCAAGATCTGATCACCGAGGGCGGCCGGGGCAGTTTCGCCTTCAGCAACCTGCCGCGCAAGTTCAA
TATTTCGATCACCGGCGACCGCGAGAACTCGGCCCACGCCGAAATCAACGACATCGGTTTTATTCCGGCGCGCCGGGAGG
AACGCATCGGCTTCAACGTGCTGGTGGGCGGGGTGCTCAATTCCCAGCGCGCCACCCCGGCGCTCCCGCTCGATGCGTTT
GTGCTTCCTGAGCAGGTGGCGCCGCTGTGCGCGGCCGTCTTGCGCATCTACAGCGACCATGGCCCGCGCGAGGCGCGCAC
CCGCGCCCGGCTTATCTACTTGCTCGAAGACTGGGGGATCTCGAAGTTTCGGGCCGCCGTCGAAGCCGACCTGGGCTTTG
CGCTCCCGCCTGCCGCCCACGAAGAATTTATCGATTACGACAAGCGCGACCACGTCGGTGTCTGGCCCCAGAAACAGGCG
GGCTACTGCTTTGTCGGCCTGCATGTGCCCATCGGCCGGCTGTGGGCGCACCAGGCGCTCGAAGTGGCCGACCTCGCCGA
GGTTTACGGTCAGGGCGAAGTGCGCCTTACCGTCGAGCAGAGCTTTTTGATCCCCCACGTCGCCGAGGAGAAGCTGGAGG
CCCTGCTGGTCGAGCCCCTGCTGGCAGTGTTTTCCCCCGAACCGAATCCGCTGCTGCGCGGTCTGGTCTCCTGCACGGGG
GCGCAGTTTTGCAACCTGGCCATCATCGAGACTAAGGAGCGCGCCCTGAAGCTCGCCCATGTCCTTGCAAGCGAACTGGA
TCTGCCCCAGCCTGTGCGTATCCACTGGTCGGGTTGTCCCAACTCCTGCGGCCAGCCGCAAATCGGTGATATCGGCCTGA
TCGGCACCCGCACCAAAGTGGCCGGACAGAGCGTCGATGCAGTGGACATTCTGATGGGCGGTCAGGTGGGACGGGAGGCC
CGTTTCGGCGAAGTGGTGCGCGAAGGTGTGCCTTGCGACGAATTGGCCGCCGTGCTCAAGGGGCTGCTTATCGAACACTT
CGGTGCCGAGGAGCGGCCGGTGGCCCTGGCCAGTTGA

Upstream 100 bases:

>100_bases
GACGCTGGCTCTCGAAAGGTTATTGACTCAATAACCGTTGGTTTTCGGTTGGCAATTTTTGTAGAGACGGGCACATTTTG
TTGCGCCCGGGACCGCTTCT

Downstream 100 bases:

>100_bases
GAGCTGGGGGTTTCTGCCAAAGGATCGCAAAATGCAATTCTCCAGATAGAGGCAGAACAACCGGCAAATGGCCACGATAG
GGTAGTTCCCCTGGAGCTGA

Product: ferredoxin-nitrite reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 518; Mature: 518

Protein sequence:

>518_residues
MERSCRYCLEGAVANPIEKIKDEKPGLAVKAELIEFARLGWEAISKGDLERLKWIGWFFRKKTPGRWMVRLRVPGGILSS
EQLRVVGGILAEFSDVKQLDITTRQNLEMRGLRIEDSVSIQERLEAVGLTTVQSGMDNVRNIVGSPVAGIDPDELIDVRP
LVQQMQDLITEGGRGSFAFSNLPRKFNISITGDRENSAHAEINDIGFIPARREERIGFNVLVGGVLNSQRATPALPLDAF
VLPEQVAPLCAAVLRIYSDHGPREARTRARLIYLLEDWGISKFRAAVEADLGFALPPAAHEEFIDYDKRDHVGVWPQKQA
GYCFVGLHVPIGRLWAHQALEVADLAEVYGQGEVRLTVEQSFLIPHVAEEKLEALLVEPLLAVFSPEPNPLLRGLVSCTG
AQFCNLAIIETKERALKLAHVLASELDLPQPVRIHWSGCPNSCGQPQIGDIGLIGTRTKVAGQSVDAVDILMGGQVGREA
RFGEVVREGVPCDELAAVLKGLLIEHFGAEERPVALAS

Sequences:

>Translated_518_residues
MERSCRYCLEGAVANPIEKIKDEKPGLAVKAELIEFARLGWEAISKGDLERLKWIGWFFRKKTPGRWMVRLRVPGGILSS
EQLRVVGGILAEFSDVKQLDITTRQNLEMRGLRIEDSVSIQERLEAVGLTTVQSGMDNVRNIVGSPVAGIDPDELIDVRP
LVQQMQDLITEGGRGSFAFSNLPRKFNISITGDRENSAHAEINDIGFIPARREERIGFNVLVGGVLNSQRATPALPLDAF
VLPEQVAPLCAAVLRIYSDHGPREARTRARLIYLLEDWGISKFRAAVEADLGFALPPAAHEEFIDYDKRDHVGVWPQKQA
GYCFVGLHVPIGRLWAHQALEVADLAEVYGQGEVRLTVEQSFLIPHVAEEKLEALLVEPLLAVFSPEPNPLLRGLVSCTG
AQFCNLAIIETKERALKLAHVLASELDLPQPVRIHWSGCPNSCGQPQIGDIGLIGTRTKVAGQSVDAVDILMGGQVGREA
RFGEVVREGVPCDELAAVLKGLLIEHFGAEERPVALAS
>Mature_518_residues
MERSCRYCLEGAVANPIEKIKDEKPGLAVKAELIEFARLGWEAISKGDLERLKWIGWFFRKKTPGRWMVRLRVPGGILSS
EQLRVVGGILAEFSDVKQLDITTRQNLEMRGLRIEDSVSIQERLEAVGLTTVQSGMDNVRNIVGSPVAGIDPDELIDVRP
LVQQMQDLITEGGRGSFAFSNLPRKFNISITGDRENSAHAEINDIGFIPARREERIGFNVLVGGVLNSQRATPALPLDAF
VLPEQVAPLCAAVLRIYSDHGPREARTRARLIYLLEDWGISKFRAAVEADLGFALPPAAHEEFIDYDKRDHVGVWPQKQA
GYCFVGLHVPIGRLWAHQALEVADLAEVYGQGEVRLTVEQSFLIPHVAEEKLEALLVEPLLAVFSPEPNPLLRGLVSCTG
AQFCNLAIIETKERALKLAHVLASELDLPQPVRIHWSGCPNSCGQPQIGDIGLIGTRTKVAGQSVDAVDILMGGQVGREA
RFGEVVREGVPCDELAAVLKGLLIEHFGAEERPVALAS

Specific function: This Enzyme Catalyzes The 6-Electron Reduction Of Sulfite To Sulfide. This Is One Of Several Activities Required For The Biosynthesis Of L-Cysteine From Sulfate. [C]

COG id: COG0155

COG function: function code P; Sulfite reductase, beta subunit (hemoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Escherichia coli, GI1789122, Length=476, Percent_Identity=24.1596638655462, Blast_Score=109, Evalue=5e-25,
Organism=Saccharomyces cerevisiae, GI6322597, Length=360, Percent_Identity=26.6666666666667, Blast_Score=93, Evalue=9e-20,

Paralogues:

None

Copy number: 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005117
- InterPro:   IPR006067
- InterPro:   IPR006066 [H]

Pfam domain/function: PF01077 NIR_SIR; PF03460 NIR_SIR_ferr [H]

EC number: =1.7.7.1 [H]

Molecular weight: Translated: 56917; Mature: 56917

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERSCRYCLEGAVANPIEKIKDEKPGLAVKAELIEFARLGWEAISKGDLERLKWIGWFFR
CCCCHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHH
KKTPGRWMVRLRVPGGILSSEQLRVVGGILAEFSDVKQLDITTRQNLEMRGLRIEDSVSI
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHEECCCCCCCEECCEEECCCCCH
QERLEAVGLTTVQSGMDNVRNIVGSPVAGIDPDELIDVRPLVQQMQDLITEGGRGSFAFS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHC
NLPRKFNISITGDRENSAHAEINDIGFIPARREERIGFNVLVGGVLNSQRATPALPLDAF
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHCCCCCCCCCCCCHH
VLPEQVAPLCAAVLRIYSDHGPREARTRARLIYLLEDWGISKFRAAVEADLGFALPPAAH
HCHHHHHHHHHHHHHHHHCCCCHHHHHHEEEEEEEECCCHHHHHHHHHHCCCCCCCCCHH
EEFIDYDKRDHVGVWPQKQAGYCFVGLHVPIGRLWAHQALEVADLAEVYGQGEVRLTVEQ
HHHHCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
SFLIPHVAEEKLEALLVEPLLAVFSPEPNPLLRGLVSCTGAQFCNLAIIETKERALKLAH
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHCEEEEECHHHHHHHHH
VLASELDLPQPVRIHWSGCPNSCGQPQIGDIGLIGTRTKVAGQSVDAVDILMGGQVGREA
HHHHHCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCHHHCCCCCCHHHEEECCCCCCCH
RFGEVVREGVPCDELAAVLKGLLIEHFGAEERPVALAS
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MERSCRYCLEGAVANPIEKIKDEKPGLAVKAELIEFARLGWEAISKGDLERLKWIGWFFR
CCCCHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHCHHHHCCCHHHHHHHHHHHHH
KKTPGRWMVRLRVPGGILSSEQLRVVGGILAEFSDVKQLDITTRQNLEMRGLRIEDSVSI
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCHHEECCCCCCCEECCEEECCCCCH
QERLEAVGLTTVQSGMDNVRNIVGSPVAGIDPDELIDVRPLVQQMQDLITEGGRGSFAFS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHC
NLPRKFNISITGDRENSAHAEINDIGFIPARREERIGFNVLVGGVLNSQRATPALPLDAF
CCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECHHCCCCCCCCCCCCHH
VLPEQVAPLCAAVLRIYSDHGPREARTRARLIYLLEDWGISKFRAAVEADLGFALPPAAH
HCHHHHHHHHHHHHHHHHCCCCHHHHHHEEEEEEEECCCHHHHHHHHHHCCCCCCCCCHH
EEFIDYDKRDHVGVWPQKQAGYCFVGLHVPIGRLWAHQALEVADLAEVYGQGEVRLTVEQ
HHHHCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEC
SFLIPHVAEEKLEALLVEPLLAVFSPEPNPLLRGLVSCTGAQFCNLAIIETKERALKLAH
CCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHCEEEEECHHHHHHHHH
VLASELDLPQPVRIHWSGCPNSCGQPQIGDIGLIGTRTKVAGQSVDAVDILMGGQVGREA
HHHHHCCCCCCEEEEECCCCCCCCCCCCCCEEEECCCHHHCCCCCCHHHEEECCCCCCCH
RFGEVVREGVPCDELAAVLKGLLIEHFGAEERPVALAS
HHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8490140 [H]