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The map label for this gene is hdhA [C]

Identifier: 37520992

GI number: 37520992

Start: 1528136

End: 1529101

Strand: Direct

Name: hdhA [C]

Synonym: glr1423

Alternate gene names: 37520992

Gene position: 1528136-1529101 (Clockwise)

Preceding gene: 37520991

Following gene: 37520993

Centisome position: 32.8

GC content: 56.83

Gene sequence:

>966_bases
ATGACCCAAAAATATGGACCCAAGACAACAACCGACGAAGTGCTTTCCGGCATTGAACTCAAGGGAAAGCGATTTCTGAT
TACGGGTGCATCGTCGGGCATTGGGCTTGAAACCGCCCGCTCACTGGTCTCTCACGGCGCTAGCGTCGTCGGCGCGGTCA
GAAACCTCGCTAAAGCTGAGCCAGCCACTGCATCGGTTCGTGATGCCGCGTCGCAGGGCGGCAGCTTGGAGTTGATCAAT
CTCGATCTGGCATCCTTGCAAAGCGTTCGCGCCTGTGCGGATAAACTGTTGGCTGACGGTCAACCGTTCGATGCCATCAT
CGCTAACGCTGGTGTTATGGCAACTCCGTTCGGTCGGACGATCGACGGCTTTGAAGTCCAGTTCGGGACGAACCATCTTG
GCCATTTTGCCCTGATCAATCAGATCGAGCCGCTGCTCGCCAATGATGGACGGCTGGTGGTCCTATCATCGCTCGCGCAT
CGCGGTGCCGATATCGACTTGGACGATCCGAATTTCGAGCAGCAGGCATACGATCCATGGGTCGCTTATAGCCGATCGAA
AACCGCCAATTCACTATTCGCTGTGGAGTTTGACAGGCGGTATCGCGATCGCGGCATTCGGGCTGCTTCGGTGATGCCCG
GAAACAGTCTGACGGATCTACCCCGCCATCTCTCGCCAGAGGACTTGCAGGGACTTTTGCAGACTGTTGACGCAGCGCGC
ACGGAAGCGGGTCTGCCGCCGAAAGAGTTGAAAGAAATTTCGCAGGCAGCCGCGACATCGGTCTGGGCAGCAGTCGTGGC
GAATAAAGACGAGATCGGTGGACATTATCTTGAGGATTGTGCGGTCGTGCCGATCGATGATACGCCCAACCCGTTTGCCG
ACGGCGTCAGGTCGTATGCGCTCGACGCTAACAGGGCTAAGCAACTGTGGGCGAAAAGCGAGGAATTGATCAGCGCTGCG
TCTTGA

Upstream 100 bases:

>100_bases
GAGACACATGCGGAGGAGGTGGCAATTTTGATCCGCACGTTCCTCGATAGCGCGATCGGTTCCACCACAGTGAAAATCTA
ACTCAGTAAAGGATGAAGTC

Downstream 100 bases:

>100_bases
AGGTTATCGCGCAAGAATCTGAAAGAAACCGAGCATTAGAATTCATTACAATTCGGTTAAATCAGACAAGAATCCAATGC
GGTATTCATAGGAGATTGCA

Product: oxidoreductase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: NA

Number of amino acids: Translated: 321; Mature: 320

Protein sequence:

>321_residues
MTQKYGPKTTTDEVLSGIELKGKRFLITGASSGIGLETARSLVSHGASVVGAVRNLAKAEPATASVRDAASQGGSLELIN
LDLASLQSVRACADKLLADGQPFDAIIANAGVMATPFGRTIDGFEVQFGTNHLGHFALINQIEPLLANDGRLVVLSSLAH
RGADIDLDDPNFEQQAYDPWVAYSRSKTANSLFAVEFDRRYRDRGIRAASVMPGNSLTDLPRHLSPEDLQGLLQTVDAAR
TEAGLPPKELKEISQAAATSVWAAVVANKDEIGGHYLEDCAVVPIDDTPNPFADGVRSYALDANRAKQLWAKSEELISAA
S

Sequences:

>Translated_321_residues
MTQKYGPKTTTDEVLSGIELKGKRFLITGASSGIGLETARSLVSHGASVVGAVRNLAKAEPATASVRDAASQGGSLELIN
LDLASLQSVRACADKLLADGQPFDAIIANAGVMATPFGRTIDGFEVQFGTNHLGHFALINQIEPLLANDGRLVVLSSLAH
RGADIDLDDPNFEQQAYDPWVAYSRSKTANSLFAVEFDRRYRDRGIRAASVMPGNSLTDLPRHLSPEDLQGLLQTVDAAR
TEAGLPPKELKEISQAAATSVWAAVVANKDEIGGHYLEDCAVVPIDDTPNPFADGVRSYALDANRAKQLWAKSEELISAA
S
>Mature_320_residues
TQKYGPKTTTDEVLSGIELKGKRFLITGASSGIGLETARSLVSHGASVVGAVRNLAKAEPATASVRDAASQGGSLELINL
DLASLQSVRACADKLLADGQPFDAIIANAGVMATPFGRTIDGFEVQFGTNHLGHFALINQIEPLLANDGRLVVLSSLAHR
GADIDLDDPNFEQQAYDPWVAYSRSKTANSLFAVEFDRRYRDRGIRAASVMPGNSLTDLPRHLSPEDLQGLLQTVDAART
EAGLPPKELKEISQAAATSVWAAVVANKDEIGGHYLEDCAVVPIDDTPNPFADGVRSYALDANRAKQLWAKSEELISAAS

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI7706523, Length=325, Percent_Identity=35.3846153846154, Blast_Score=173, Evalue=1e-43,
Organism=Homo sapiens, GI10190746, Length=313, Percent_Identity=35.4632587859425, Blast_Score=154, Evalue=1e-37,
Organism=Homo sapiens, GI225579078, Length=300, Percent_Identity=34.3333333333333, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI186928839, Length=304, Percent_Identity=33.8815789473684, Blast_Score=141, Evalue=6e-34,
Organism=Homo sapiens, GI166795268, Length=305, Percent_Identity=33.4426229508197, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI19923931, Length=240, Percent_Identity=35.4166666666667, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI193804850, Length=307, Percent_Identity=31.2703583061889, Blast_Score=118, Evalue=6e-27,
Organism=Homo sapiens, GI7705421, Length=246, Percent_Identity=31.3008130081301, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI146231950, Length=306, Percent_Identity=32.3529411764706, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI193207620, Length=327, Percent_Identity=33.3333333333333, Blast_Score=149, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17532805, Length=326, Percent_Identity=33.7423312883436, Blast_Score=146, Evalue=1e-35,
Organism=Caenorhabditis elegans, GI17532791, Length=328, Percent_Identity=31.0975609756098, Blast_Score=134, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17535059, Length=323, Percent_Identity=31.2693498452012, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI71992402, Length=323, Percent_Identity=30.3405572755418, Blast_Score=117, Evalue=8e-27,
Organism=Caenorhabditis elegans, GI32565532, Length=227, Percent_Identity=31.2775330396476, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17505416, Length=210, Percent_Identity=30.4761904761905, Blast_Score=100, Evalue=9e-22,
Organism=Caenorhabditis elegans, GI17558006, Length=300, Percent_Identity=27.6666666666667, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI72001364, Length=268, Percent_Identity=30.2238805970149, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI115533172, Length=194, Percent_Identity=29.3814432989691, Blast_Score=65, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI6324820, Length=214, Percent_Identity=32.2429906542056, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI20129347, Length=323, Percent_Identity=34.3653250773994, Blast_Score=165, Evalue=4e-41,
Organism=Drosophila melanogaster, GI22024069, Length=298, Percent_Identity=36.241610738255, Blast_Score=147, Evalue=7e-36,
Organism=Drosophila melanogaster, GI24586328, Length=296, Percent_Identity=33.445945945946, Blast_Score=135, Evalue=4e-32,
Organism=Drosophila melanogaster, GI28573296, Length=296, Percent_Identity=33.1081081081081, Blast_Score=127, Evalue=9e-30,
Organism=Drosophila melanogaster, GI45554248, Length=219, Percent_Identity=39.2694063926941, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24640117, Length=219, Percent_Identity=39.2694063926941, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI19921754, Length=303, Percent_Identity=33.003300330033, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI161076371, Length=298, Percent_Identity=32.8859060402685, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24647946, Length=331, Percent_Identity=27.190332326284, Blast_Score=96, Evalue=3e-20,
Organism=Drosophila melanogaster, GI45553409, Length=312, Percent_Identity=28.2051282051282, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24647948, Length=312, Percent_Identity=28.2051282051282, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24656154, Length=263, Percent_Identity=31.5589353612167, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19922628, Length=263, Percent_Identity=31.5589353612167, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24647931, Length=289, Percent_Identity=29.4117647058824, Blast_Score=85, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 34167; Mature: 34035

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQKYGPKTTTDEVLSGIELKGKRFLITGASSGIGLETARSLVSHGASVVGAVRNLAKAE
CCCCCCCCCCHHHHHCCCEECCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCC
PATASVRDAASQGGSLELINLDLASLQSVRACADKLLADGQPFDAIIANAGVMATPFGRT
CCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEECCCCCC
IDGFEVQFGTNHLGHFALINQIEPLLANDGRLVVLSSLAHRGADIDLDDPNFEQQAYDPW
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCCCCCCCCHHHHCCCH
VAYSRSKTANSLFAVEFDRRYRDRGIRAASVMPGNSLTDLPRHLSPEDLQGLLQTVDAAR
HHHCCCCCCCCEEEEEHHHHHHHCCCEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHH
TEAGLPPKELKEISQAAATSVWAAVVANKDEIGGHYLEDCAVVPIDDTPNPFADGVRSYA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEHHCCEEEECCCCCCHHHHHHHHHH
LDANRAKQLWAKSEELISAAS
CCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TQKYGPKTTTDEVLSGIELKGKRFLITGASSGIGLETARSLVSHGASVVGAVRNLAKAE
CCCCCCCCCHHHHHCCCEECCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHCC
PATASVRDAASQGGSLELINLDLASLQSVRACADKLLADGQPFDAIIANAGVMATPFGRT
CCHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEECCCCCC
IDGFEVQFGTNHLGHFALINQIEPLLANDGRLVVLSSLAHRGADIDLDDPNFEQQAYDPW
CCCEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEEEHHHHCCCCCCCCCCCCHHHHCCCH
VAYSRSKTANSLFAVEFDRRYRDRGIRAASVMPGNSLTDLPRHLSPEDLQGLLQTVDAAR
HHHCCCCCCCCEEEEEHHHHHHHCCCEEEEECCCCCHHHHHCCCCHHHHHHHHHHHHHHH
TEAGLPPKELKEISQAAATSVWAAVVANKDEIGGHYLEDCAVVPIDDTPNPFADGVRSYA
HHCCCCHHHHHHHHHHHHHHHHHHHHCCCHHCCCEEHHCCEEEECCCCCCHHHHHHHHHH
LDANRAKQLWAKSEELISAAS
CCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8383668 [H]