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The map label for this gene is potB [H]

Identifier: 37520972

GI number: 37520972

Start: 1503465

End: 1504319

Strand: Reverse

Name: potB [H]

Synonym: gll1403

Alternate gene names: 37520972

Gene position: 1504319-1503465 (Counterclockwise)

Preceding gene: 37520973

Following gene: 37520971

Centisome position: 32.29

GC content: 60.94

Gene sequence:

>855_bases
TTGAAAACCTCTGCCTTCTCCCCCCTGCGGGGCATTCTGCTCACCGCCCCAGCCGTCATGTATCTACTGGCTTTCCTCGC
GGCTCCCCTTGCCCTGGTGGCCGCTACGAGTGTGCTGAGCCGTAGCCCCTACGGCGAAGTGGTCTTCAAATTCACCGCCG
ACAACTACACCCGTCTGTTCGATCCTCTCTATCTCAACATCCTGCTCGATTCGCTGACGATTGCCGGGGCGACAACGGCC
ATCACGTTGCTCATCGGCTATCCGCTCGCTTGGTGGATGGCCCGCGTCCCGCAACGGGTGCGCTCGGTGGCGCTGTTTGC
CATTCTGGTACCGTTCTGGACAAATTTTTTGATCCGCATCTACGCCTGGATCTTAATTCTGCGTTCTGGGGGACTGCTTG
AAGGGCTGCTTGCGGGATTGGGGCTCTGGCGCGGTTCGATCGAAATTCTCTACACTCCCTGGGCGGTGCTCATCGGTATG
GTCTACGAGTTTTTGCCCTTCATGGTGCTGCCGCTCTACGCCAGCCTCGAAAAGATCGACGAAGCGCAGCTCGCCGCCGC
CGCCGATCTTGGCGCCAGACCGTTCCAGGTATTCTGGCGCGTCGTGCTGCCCCTGGGACTACCGGGCCTGGTGGCGGGCA
GCACCCTGGTTTTTGTCCCGGCGATGGGCATGTTCGCCGTGCCCGATTTGATGGGTGGGGCACGCACGTTGCTGGTGGGC
AATTTGATCCGCAACCAGTTTCTAGTCGCACGCGACTGGCCCTTTGGAGCGGCGGCGGCCATGGTGCTGGTGATCCTCAC
CCTCGGGCTGCTGGTCCTCTACACCCGCTTTGCCGAGAAGGAAACACTCGTATGA

Upstream 100 bases:

>100_bases
TGTTGCTGACCAATCGCAATGGCCGGGAAGTATTGGCGGAGGGAGCGGCGTGTGTTTTGAGCGCTGATTGTCATGACACG
ATCGTGCTTGCGGAGGGGAA

Downstream 100 bases:

>100_bases
GCGGCAGGCGGGCATTGTGGCTCAGGCTGTGGGCCGTGGCGGTCTTCGGTTATCTATACCTGCCCATCTTGGTGTTGGTG
GTATTTTCCTTCAACAGCCA

Product: ABC transporter permease protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKTSAFSPLRGILLTAPAVMYLLAFLAAPLALVAATSVLSRSPYGEVVFKFTADNYTRLFDPLYLNILLDSLTIAGATTA
ITLLIGYPLAWWMARVPQRVRSVALFAILVPFWTNFLIRIYAWILILRSGGLLEGLLAGLGLWRGSIEILYTPWAVLIGM
VYEFLPFMVLPLYASLEKIDEAQLAAAADLGARPFQVFWRVVLPLGLPGLVAGSTLVFVPAMGMFAVPDLMGGARTLLVG
NLIRNQFLVARDWPFGAAAAMVLVILTLGLLVLYTRFAEKETLV

Sequences:

>Translated_284_residues
MKTSAFSPLRGILLTAPAVMYLLAFLAAPLALVAATSVLSRSPYGEVVFKFTADNYTRLFDPLYLNILLDSLTIAGATTA
ITLLIGYPLAWWMARVPQRVRSVALFAILVPFWTNFLIRIYAWILILRSGGLLEGLLAGLGLWRGSIEILYTPWAVLIGM
VYEFLPFMVLPLYASLEKIDEAQLAAAADLGARPFQVFWRVVLPLGLPGLVAGSTLVFVPAMGMFAVPDLMGGARTLLVG
NLIRNQFLVARDWPFGAAAAMVLVILTLGLLVLYTRFAEKETLV
>Mature_284_residues
MKTSAFSPLRGILLTAPAVMYLLAFLAAPLALVAATSVLSRSPYGEVVFKFTADNYTRLFDPLYLNILLDSLTIAGATTA
ITLLIGYPLAWWMARVPQRVRSVALFAILVPFWTNFLIRIYAWILILRSGGLLEGLLAGLGLWRGSIEILYTPWAVLIGM
VYEFLPFMVLPLYASLEKIDEAQLAAAADLGARPFQVFWRVVLPLGLPGLVAGSTLVFVPAMGMFAVPDLMGGARTLLVG
NLIRNQFLVARDWPFGAAAAMVLVILTLGLLVLYTRFAEKETLV

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=256, Percent_Identity=50, Blast_Score=249, Evalue=1e-67,
Organism=Escherichia coli, GI1787080, Length=238, Percent_Identity=40.7563025210084, Blast_Score=195, Evalue=3e-51,
Organism=Escherichia coli, GI1787713, Length=247, Percent_Identity=33.6032388663968, Blast_Score=127, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31054; Mature: 31054

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSAFSPLRGILLTAPAVMYLLAFLAAPLALVAATSVLSRSPYGEVVFKFTADNYTRLF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHH
DPLYLNILLDSLTIAGATTAITLLIGYPLAWWMARVPQRVRSVALFAILVPFWTNFLIRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAWILILRSGGLLEGLLAGLGLWRGSIEILYTPWAVLIGMVYEFLPFMVLPLYASLEKID
HHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAQLAAAADLGARPFQVFWRVVLPLGLPGLVAGSTLVFVPAMGMFAVPDLMGGARTLLVG
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHH
NLIRNQFLVARDWPFGAAAAMVLVILTLGLLVLYTRFAEKETLV
HHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTSAFSPLRGILLTAPAVMYLLAFLAAPLALVAATSVLSRSPYGEVVFKFTADNYTRLF
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCHHHHH
DPLYLNILLDSLTIAGATTAITLLIGYPLAWWMARVPQRVRSVALFAILVPFWTNFLIRI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YAWILILRSGGLLEGLLAGLGLWRGSIEILYTPWAVLIGMVYEFLPFMVLPLYASLEKID
HHHHHHHHCCCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHH
EAQLAAAADLGARPFQVFWRVVLPLGLPGLVAGSTLVFVPAMGMFAVPDLMGGARTLLVG
HHHHHHHHHCCCCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHCCHHHHHHH
NLIRNQFLVARDWPFGAAAAMVLVILTLGLLVLYTRFAEKETLV
HHHHHCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]