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The map label for this gene is dhaAF [H]

Identifier: 37520850

GI number: 37520850

Start: 1362337

End: 1363242

Strand: Reverse

Name: dhaAF [H]

Synonym: gll1281

Alternate gene names: 37520850

Gene position: 1363242-1362337 (Counterclockwise)

Preceding gene: 37520856

Following gene: 37520849

Centisome position: 29.26

GC content: 62.91

Gene sequence:

>906_bases
GTGCGCCCTCGGTGTCCAAACCATCCCCACAACCGGAGAGACAAGATGACGATGTACCGTTACGCCACCGTCGATGGTCT
GCGCGTGTTTTACCGCGAGGCCGGTCCCAAGGACGCTCCCGCCGTGCTGCTGCTGCACGGTTTTCCGACCTCCTCGCACA
TGTACCGCGATTTGATCCCGCTGCTTGCCGACCGCTACCGGGTGGTGGCGCCGGATCTGCCGGGTTTCGGCTTTACCGAC
GCGCCCGAACGCGGACAGTTCACCTACTCGTTCGACCGTCTGGCCCATGTGATCGACCGGTTTAGCGAGGCTGTGGACCT
GGGGCGTTACGCGCTCTATATCTTCGATTACGGTGCGCCGGTCGGTCTGCGCCTTGCCAGCGCTCATCCCGAGCGGATCA
CAGCCCTTATCACCCAAAACGGCAACGCCTACGAAGAAGGCCTTAGCGCAGGCTGGAATCCAATCCAGTGCTACTGGCAG
GAACCGACGCCCGAGAACCGCTCGGCGCTGCGCGATTTTCTCACACCCGAAGCGACCCAATCGCAGTACGCCCACGGCGT
GGCGGACCTTTCGCTGATCGCCCCTGAGGCTTACACCCTCGATTCGGCTTTGTTGAGTCGGCCGGGCAACGAGGAGATCC
AACTCGATTTGTTTTTGGATTACCGCAGCAACGTCGCGCTCTATCCCGCCTTCCAGGCTTACTTTCGCGCCCACCGGCCG
CCCACCCTCGCCGTCTGGGGCAACAACGACCCGTTCTTCTTGCCGGCCGGCGCCGAGGCGTTCAAGCGCGACAATTCCGA
AGCTGAGGTACACCTTTTCGACACCGGCCACTTCGCCCTGGAGACCCACGCCCGGGTGATCGCCGAGCGGATCCGCGATT
TTTTGGACCGCCAACTGCGCGGCTGA

Upstream 100 bases:

>100_bases
TGCCCATTCTTGCATAACCATCAAAAAATAACTTGGCCGGTTATCAATCTCATGCTAACCTTCAAAATAGGGCAAAGAGG
GTTAGCGCAGCATCTGGGGT

Downstream 100 bases:

>100_bases
ACCCGTACCGTCGGCGACGGTGTTGCATAATGTTGCGCGCCGCCGGGGCTGGACGTATCGTGGCAGCAGCGTTCCGTCAG
CCGATGTTTAGCGCCGAACA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MRPRCPNHPHNRRDKMTMYRYATVDGLRVFYREAGPKDAPAVLLLHGFPTSSHMYRDLIPLLADRYRVVAPDLPGFGFTD
APERGQFTYSFDRLAHVIDRFSEAVDLGRYALYIFDYGAPVGLRLASAHPERITALITQNGNAYEEGLSAGWNPIQCYWQ
EPTPENRSALRDFLTPEATQSQYAHGVADLSLIAPEAYTLDSALLSRPGNEEIQLDLFLDYRSNVALYPAFQAYFRAHRP
PTLAVWGNNDPFFLPAGAEAFKRDNSEAEVHLFDTGHFALETHARVIAERIRDFLDRQLRG

Sequences:

>Translated_301_residues
MRPRCPNHPHNRRDKMTMYRYATVDGLRVFYREAGPKDAPAVLLLHGFPTSSHMYRDLIPLLADRYRVVAPDLPGFGFTD
APERGQFTYSFDRLAHVIDRFSEAVDLGRYALYIFDYGAPVGLRLASAHPERITALITQNGNAYEEGLSAGWNPIQCYWQ
EPTPENRSALRDFLTPEATQSQYAHGVADLSLIAPEAYTLDSALLSRPGNEEIQLDLFLDYRSNVALYPAFQAYFRAHRP
PTLAVWGNNDPFFLPAGAEAFKRDNSEAEVHLFDTGHFALETHARVIAERIRDFLDRQLRG
>Mature_301_residues
MRPRCPNHPHNRRDKMTMYRYATVDGLRVFYREAGPKDAPAVLLLHGFPTSSHMYRDLIPLLADRYRVVAPDLPGFGFTD
APERGQFTYSFDRLAHVIDRFSEAVDLGRYALYIFDYGAPVGLRLASAHPERITALITQNGNAYEEGLSAGWNPIQCYWQ
EPTPENRSALRDFLTPEATQSQYAHGVADLSLIAPEAYTLDSALLSRPGNEEIQLDLFLDYRSNVALYPAFQAYFRAHRP
PTLAVWGNNDPFFLPAGAEAFKRDNSEAEVHLFDTGHFALETHARVIAERIRDFLDRQLRG

Specific function: Catalyzes hydrolytic cleavage of carbon-halogen bonds in halogenated aliphatic compounds, leading to the formation of the corresponding primary alcohols, halide ions and protons. Has a broad substrate specificity, which includes mono- and di- chlorinated

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the haloalkane dehalogenase family. Type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI218777837, Length=295, Percent_Identity=27.7966101694915, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI218777840, Length=288, Percent_Identity=25, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI13376164, Length=288, Percent_Identity=25, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI87081721, Length=280, Percent_Identity=23.5714285714286, Blast_Score=69, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17562298, Length=289, Percent_Identity=23.1833910034602, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17554012, Length=295, Percent_Identity=21.6949152542373, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324392, Length=271, Percent_Identity=50.5535055350554, Blast_Score=276, Evalue=4e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR000639 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =3.8.1.5 [H]

Molecular weight: Translated: 34192; Mature: 34192

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRCPNHPHNRRDKMTMYRYATVDGLRVFYREAGPKDAPAVLLLHGFPTSSHMYRDLIP
CCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHH
LLADRYRVVAPDLPGFGFTDAPERGQFTYSFDRLAHVIDRFSEAVDLGRYALYIFDYGAP
HHHCCCEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
VGLRLASAHPERITALITQNGNAYEEGLSAGWNPIQCYWQEPTPENRSALRDFLTPEATQ
CCEEEECCCCCCEEEEEECCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCHH
SQYAHGVADLSLIAPEAYTLDSALLSRPGNEEIQLDLFLDYRSNVALYPAFQAYFRAHRP
HHHHHCHHHHEEECCCHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEHHHHHHHHHCCC
PTLAVWGNNDPFFLPAGAEAFKRDNSEAEVHLFDTGHFALETHARVIAERIRDFLDRQLR
CEEEEECCCCCEEEECCHHHHHCCCCCCEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHC
G
C
>Mature Secondary Structure
MRPRCPNHPHNRRDKMTMYRYATVDGLRVFYREAGPKDAPAVLLLHGFPTSSHMYRDLIP
CCCCCCCCCCCCCCCEEEEEEEHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHHHHHH
LLADRYRVVAPDLPGFGFTDAPERGQFTYSFDRLAHVIDRFSEAVDLGRYALYIFDYGAP
HHHCCCEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCEEEEEEECCCC
VGLRLASAHPERITALITQNGNAYEEGLSAGWNPIQCYWQEPTPENRSALRDFLTPEATQ
CCEEEECCCCCCEEEEEECCCCHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHCCCCCHH
SQYAHGVADLSLIAPEAYTLDSALLSRPGNEEIQLDLFLDYRSNVALYPAFQAYFRAHRP
HHHHHCHHHHEEECCCHHHHHHHHHCCCCCCCEEEEEEEECCCCEEEEHHHHHHHHHCCC
PTLAVWGNNDPFFLPAGAEAFKRDNSEAEVHLFDTGHFALETHARVIAERIRDFLDRQLR
CEEEEECCCCCEEEECCHHHHHCCCCCCEEEEEECCCEEHHHHHHHHHHHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10094681; 10735862 [H]