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The map label for this gene is pdhC [H]

Identifier: 37520661

GI number: 37520661

Start: 1169697

End: 1170851

Strand: Reverse

Name: pdhC [H]

Synonym: gll1092

Alternate gene names: 37520661

Gene position: 1170851-1169697 (Counterclockwise)

Preceding gene: 37520662

Following gene: 37520660

Centisome position: 25.13

GC content: 49.0

Gene sequence:

>1155_bases
ATGGTCATCACAGAAATCAAGATTCCCCAGTTGGGAGAGGGTTTGCAGGAAGTTTTGATTGACAGACTACTCAAGCGCTC
CGGTGAGCACATCAAGCGGGATGAGGCAATCTACGTCATAGAGACGGACAAAGCTCTGATGGATGTGGAGTCACCTTATG
AAGGAGTGATTCAAGAATGGCTGGTGGAAGAAAATGATGTTGTCCTCGTTGGAAGTCCAGTTGCACGGATCCAAACCATT
ATCGAGCATTCGGCCGCCCCGCACTTGGAGCCACGAAAAGCGGAGTTTCCTGAAACGAAGCCAAAATCCCAGATTGCCCC
TTCCTCTTCGGCCGTAATTCCGCCACGAACCCGCGCCTACTGTAAGCAGTTGGGGATCCCTGAAGATGAGATGCTCCAGA
TCTCAGCTCTAACGGAAAAGCTGATGCCACGGGATGTAGACAAATACGTTGCTGCGAAGTCAAACCCAGAAATTTTACCG
AGTCTGGAGCAAGTTCGTTGCGATTACCTGGAGCGCTCTATATCCGGCAAACAGAAAACACTGCTCTATCGACTCAAGCG
CAGCACTGAATTGGTAGTGCCGGGGACCATTTCACGCCCAGTGGCTTGGTTTCGCCTTCAAGAAGGGATTCAGATTCTCA
CGAACAGAGGAGATTCTCCTTTGCCTTCTGAATTTCAGATGTTCGCCTATTGTGTAGCACAAGCAAGTAAGAAACACCCC
AAATTTCGTTCTGTTTTGACAGGTGAAAATTCGGTTCGAGAGTACCGTCTTCTAAACCTGGGGATTGCGATTGCCAGACC
CAATGACGAACTAATTACGGCAGTGATCCCTGAGGCAGACTGTTTGAGCCCTGCAGCATTCGCAAATACAGCACGGCGAC
AGATGAGAAAAGCAATCAAAGAAGGAGATCAAGCTTCTGAGTCAACCCAAATCACCTTGTCATACCTTGGTGAATATGGA
ATTGTTGACGCAACACCTGCCCTGGTAGCCCCTGCAGTCGCTGTCATGTTCATCGGCTCAACCTATGGGCAGGCGGGTAA
ACAGGTTGCCAACATAGCATTGACTTTCGATCATCGCTTGATCAACGGAGCTGGGGCCGCACTCTTTTTGAATGATATCG
CTCAGCAAGTCGAATCTTTTTCAAGTGAATTATGA

Upstream 100 bases:

>100_bases
GGATGTGCCTATTCCTTACAATCCAGTCTTGGAGTATGCCGTGCTGCCCGACTTGACAAGGGTGTTAGAAGCAATTTATA
CAACTCTAGAGTAACGGCAG

Downstream 100 bases:

>100_bases
ATAAAACGGCTAAGTTGTCTGACAGCCCAGACGGCCCAGGTTTGCTGCAAGCCTAACCGAAGGTTGGTGTAGCAATAACC
AGCTTAAGAAACTTTTCACT

Product: branched-chain alpha-keto acid dehydrogenase subunit E2

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 384; Mature: 384

Protein sequence:

>384_residues
MVITEIKIPQLGEGLQEVLIDRLLKRSGEHIKRDEAIYVIETDKALMDVESPYEGVIQEWLVEENDVVLVGSPVARIQTI
IEHSAAPHLEPRKAEFPETKPKSQIAPSSSAVIPPRTRAYCKQLGIPEDEMLQISALTEKLMPRDVDKYVAAKSNPEILP
SLEQVRCDYLERSISGKQKTLLYRLKRSTELVVPGTISRPVAWFRLQEGIQILTNRGDSPLPSEFQMFAYCVAQASKKHP
KFRSVLTGENSVREYRLLNLGIAIARPNDELITAVIPEADCLSPAAFANTARRQMRKAIKEGDQASESTQITLSYLGEYG
IVDATPALVAPAVAVMFIGSTYGQAGKQVANIALTFDHRLINGAGAALFLNDIAQQVESFSSEL

Sequences:

>Translated_384_residues
MVITEIKIPQLGEGLQEVLIDRLLKRSGEHIKRDEAIYVIETDKALMDVESPYEGVIQEWLVEENDVVLVGSPVARIQTI
IEHSAAPHLEPRKAEFPETKPKSQIAPSSSAVIPPRTRAYCKQLGIPEDEMLQISALTEKLMPRDVDKYVAAKSNPEILP
SLEQVRCDYLERSISGKQKTLLYRLKRSTELVVPGTISRPVAWFRLQEGIQILTNRGDSPLPSEFQMFAYCVAQASKKHP
KFRSVLTGENSVREYRLLNLGIAIARPNDELITAVIPEADCLSPAAFANTARRQMRKAIKEGDQASESTQITLSYLGEYG
IVDATPALVAPAVAVMFIGSTYGQAGKQVANIALTFDHRLINGAGAALFLNDIAQQVESFSSEL
>Mature_384_residues
MVITEIKIPQLGEGLQEVLIDRLLKRSGEHIKRDEAIYVIETDKALMDVESPYEGVIQEWLVEENDVVLVGSPVARIQTI
IEHSAAPHLEPRKAEFPETKPKSQIAPSSSAVIPPRTRAYCKQLGIPEDEMLQISALTEKLMPRDVDKYVAAKSNPEILP
SLEQVRCDYLERSISGKQKTLLYRLKRSTELVVPGTISRPVAWFRLQEGIQILTNRGDSPLPSEFQMFAYCVAQASKKHP
KFRSVLTGENSVREYRLLNLGIAIARPNDELITAVIPEADCLSPAAFANTARRQMRKAIKEGDQASESTQITLSYLGEYG
IVDATPALVAPAVAVMFIGSTYGQAGKQVANIALTFDHRLINGAGAALFLNDIAQQVESFSSEL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6320352, Length=403, Percent_Identity=26.0545905707196, Blast_Score=79, Evalue=2e-15,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 42438; Mature: 42438

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS50968 BIOTINYL_LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVITEIKIPQLGEGLQEVLIDRLLKRSGEHIKRDEAIYVIETDKALMDVESPYEGVIQEW
CEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHCCCCCHHHHHHHH
LVEENDVVLVGSPVARIQTIIEHSAAPHLEPRKAEFPETKPKSQIAPSSSAVIPPRTRAY
HCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
CKQLGIPEDEMLQISALTEKLMPRDVDKYVAAKSNPEILPSLEQVRCDYLERSISGKQKT
HHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHH
LLYRLKRSTELVVPGTISRPVAWFRLQEGIQILTNRGDSPLPSEFQMFAYCVAQASKKHP
HHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
KFRSVLTGENSVREYRLLNLGIAIARPNDELITAVIPEADCLSPAAFANTARRQMRKAIK
HHHHHHCCCCHHHHEEEEEEEEEEECCCCCEEEEECCCCHHCCHHHHHHHHHHHHHHHHH
EGDQASESTQITLSYLGEYGIVDATPALVAPAVAVMFIGSTYGQAGKQVANIALTFDHRL
CCCCCCCCCEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHH
INGAGAALFLNDIAQQVESFSSEL
HCCCCHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MVITEIKIPQLGEGLQEVLIDRLLKRSGEHIKRDEAIYVIETDKALMDVESPYEGVIQEW
CEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCHHHHCCCCCHHHHHHHH
LVEENDVVLVGSPVARIQTIIEHSAAPHLEPRKAEFPETKPKSQIAPSSSAVIPPRTRAY
HCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHH
CKQLGIPEDEMLQISALTEKLMPRDVDKYVAAKSNPEILPSLEQVRCDYLERSISGKQKT
HHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHH
LLYRLKRSTELVVPGTISRPVAWFRLQEGIQILTNRGDSPLPSEFQMFAYCVAQASKKHP
HHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCC
KFRSVLTGENSVREYRLLNLGIAIARPNDELITAVIPEADCLSPAAFANTARRQMRKAIK
HHHHHHCCCCHHHHEEEEEEEEEEECCCCCEEEEECCCCHHCCHHHHHHHHHHHHHHHHH
EGDQASESTQITLSYLGEYGIVDATPALVAPAVAVMFIGSTYGQAGKQVANIALTFDHRL
CCCCCCCCCEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECHHH
INGAGAALFLNDIAQQVESFSSEL
HCCCCHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7569993 [H]