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The map label for this gene is capI [H]

Identifier: 37520637

GI number: 37520637

Start: 1145461

End: 1146507

Strand: Direct

Name: capI [H]

Synonym: glr1068

Alternate gene names: 37520637

Gene position: 1145461-1146507 (Clockwise)

Preceding gene: 37520636

Following gene: 37520638

Centisome position: 24.59

GC content: 62.37

Gene sequence:

>1047_bases
ATGAAAGTCTTAATCACCGGGGTAGCCGGCTTCATCGGCTACCACCTGGCGGCGCGTCTGCTCCAGGAAGGCAGCAAAGT
CTACGGCATCGACAACCTCAACAGCTATTACGACGTGCGCCTCAAAGAAGCGCGGCTCGCGCGGCTGGTGCCGCACCCGC
AATTTACCTTCCGGCACCTCGATATCGCCCGCCGCCCGGCCATGTTCGAACTATTTGAAAGCGAATCGTTCGACTGTGTC
GTCCACCTGGCGGCCCAGGCAGGCGTGCGCTATTCGCTCAAAAATCCCTTCGCCTACGTCGACAGCAACCTGAGCGGCTT
CGTCAACCTGCTGGAGTGCTGCCGCACCAGCGGCATCGGGCATCTGGTCTACGCCTCCTCCAGTTCGGTCTACGGCGCCA
ACACCAAAGCACCCTTCTCGGTGAGCGACAACGTCGATCACCCGGTCTCGCTCTACGCCGCCACCAAAAAAGCGAACGAG
TTGATGGCCCACGCCTACAGCCACCTGTACGCCCTGCCGACCACGGGCCTGCGCTTTTTTACCGTCTACGGCCCCTGGGG
ACGGCCGGACATGGCCTATTTCAAGTTCGTCCAGGCCATCGAAGCAGGCAAGCCCATCGACGTCTACAACCACGGCCACA
TGCAGCGCGACTTCACCTATATCGACGACATCGTCGAGGGCATCGTCCGCCTCCTCCCCCGCGTTCCCACCCATGCAGGA
GCTGCCCCCTACCGCATCTACAACATCGGCAACCACCAGCCGGTGAGTCTGATTGAATTTATCGAAGTCATCGAGCAGGC
CCTGGGCAAGCGGGCCGTGAAAAACCTGCTGCCGATGCAACCTGGCGATGTGCCCGCCACCTGCGCCGATGTGGACGACC
TGATGCGCGAAGTCGGCTTCAAACCGAGCACCCCACTCACCGTCGGCATCGAGCGCTTCGTCTGCTGGTACCGCGATTAC
CTGAGTGCCGCTTCGCCCGTTGTGCGCACCACCACCACCGTCCGCCCGCGATGGCCCGCCGTAGCGCCCGTCGAAGTCGC
GGGTTAG

Upstream 100 bases:

>100_bases
CGCGGGCGGGCGCTACGCCGCCCTCTACCGGTGTCAGGTGCCGGCCGCCGCCTCGGGCAAGCCCTGACACCATCCACACC
TACATCCAGCGAGTCAAGCC

Downstream 100 bases:

>100_bases
CCGCCCTGCTCGCCTCGCTGTTGCTCGGGGCGGAGCCACTGACAGCTGAGCCGGACCCTGCCGTCTCGCAGCTGGCGGAT
GTGCGCTCAGGCGAGTGGGC

Product: nucleotide sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKVLITGVAGFIGYHLAARLLQEGSKVYGIDNLNSYYDVRLKEARLARLVPHPQFTFRHLDIARRPAMFELFESESFDCV
VHLAAQAGVRYSLKNPFAYVDSNLSGFVNLLECCRTSGIGHLVYASSSSVYGANTKAPFSVSDNVDHPVSLYAATKKANE
LMAHAYSHLYALPTTGLRFFTVYGPWGRPDMAYFKFVQAIEAGKPIDVYNHGHMQRDFTYIDDIVEGIVRLLPRVPTHAG
AAPYRIYNIGNHQPVSLIEFIEVIEQALGKRAVKNLLPMQPGDVPATCADVDDLMREVGFKPSTPLTVGIERFVCWYRDY
LSAASPVVRTTTTVRPRWPAVAPVEVAG

Sequences:

>Translated_348_residues
MKVLITGVAGFIGYHLAARLLQEGSKVYGIDNLNSYYDVRLKEARLARLVPHPQFTFRHLDIARRPAMFELFESESFDCV
VHLAAQAGVRYSLKNPFAYVDSNLSGFVNLLECCRTSGIGHLVYASSSSVYGANTKAPFSVSDNVDHPVSLYAATKKANE
LMAHAYSHLYALPTTGLRFFTVYGPWGRPDMAYFKFVQAIEAGKPIDVYNHGHMQRDFTYIDDIVEGIVRLLPRVPTHAG
AAPYRIYNIGNHQPVSLIEFIEVIEQALGKRAVKNLLPMQPGDVPATCADVDDLMREVGFKPSTPLTVGIERFVCWYRDY
LSAASPVVRTTTTVRPRWPAVAPVEVAG
>Mature_348_residues
MKVLITGVAGFIGYHLAARLLQEGSKVYGIDNLNSYYDVRLKEARLARLVPHPQFTFRHLDIARRPAMFELFESESFDCV
VHLAAQAGVRYSLKNPFAYVDSNLSGFVNLLECCRTSGIGHLVYASSSSVYGANTKAPFSVSDNVDHPVSLYAATKKANE
LMAHAYSHLYALPTTGLRFFTVYGPWGRPDMAYFKFVQAIEAGKPIDVYNHGHMQRDFTYIDDIVEGIVRLLPRVPTHAG
AAPYRIYNIGNHQPVSLIEFIEVIEQALGKRAVKNLLPMQPGDVPATCADVDDLMREVGFKPSTPLTVGIERFVCWYRDY
LSAASPVVRTTTTVRPRWPAVAPVEVAG

Specific function: Required for the biosynthesis of type 1 capsular polysaccharide [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=329, Percent_Identity=27.6595744680851, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI42516563, Length=331, Percent_Identity=27.4924471299094, Blast_Score=108, Evalue=6e-24,
Organism=Homo sapiens, GI56237023, Length=339, Percent_Identity=27.4336283185841, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI56118217, Length=339, Percent_Identity=27.4336283185841, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI189083684, Length=339, Percent_Identity=27.4336283185841, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1788353, Length=345, Percent_Identity=28.1159420289855, Blast_Score=115, Evalue=5e-27,
Organism=Escherichia coli, GI48994969, Length=339, Percent_Identity=27.7286135693215, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI1786974, Length=321, Percent_Identity=28.0373831775701, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1788589, Length=295, Percent_Identity=26.1016949152542, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI71982038, Length=331, Percent_Identity=25.6797583081571, Blast_Score=105, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71982035, Length=329, Percent_Identity=25.8358662613982, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17539532, Length=328, Percent_Identity=26.219512195122, Blast_Score=98, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17568069, Length=338, Percent_Identity=25.1479289940828, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI115532424, Length=328, Percent_Identity=23.780487804878, Blast_Score=81, Evalue=9e-16,
Organism=Saccharomyces cerevisiae, GI6319493, Length=339, Percent_Identity=25.3687315634218, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI21356223, Length=332, Percent_Identity=26.2048192771084, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI19923002, Length=336, Percent_Identity=27.3809523809524, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 38690; Mature: 38690

Theoretical pI: Translated: 8.20; Mature: 8.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLITGVAGFIGYHLAARLLQEGSKVYGIDNLNSYYDVRLKEARLARLVPHPQFTFRHL
CEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEHHHHHHHHCCCCCHHHHHH
DIARRPAMFELFESESFDCVVHLAAQAGVRYSLKNPFAYVDSNLSGFVNLLECCRTSGIG
HHHHCCHHHHHHHCCCCHHEEEEHHHCCCEEECCCCCEEECCCHHHHHHHHHHHHHCCCC
HLVYASSSSVYGANTKAPFSVSDNVDHPVSLYAATKKANELMAHAYSHLYALPTTGLRFF
EEEEECCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHEECCCCCEEEE
TVYGPWGRPDMAYFKFVQAIEAGKPIDVYNHGHMQRDFTYIDDIVEGIVRLLPRVPTHAG
EEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
AAPYRIYNIGNHQPVSLIEFIEVIEQALGKRAVKNLLPMQPGDVPATCADVDDLMREVGF
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
KPSTPLTVGIERFVCWYRDYLSAASPVVRTTTTVRPRWPAVAPVEVAG
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEECC
>Mature Secondary Structure
MKVLITGVAGFIGYHLAARLLQEGSKVYGIDNLNSYYDVRLKEARLARLVPHPQFTFRHL
CEEEEEHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEHHHHHHHHCCCCCHHHHHH
DIARRPAMFELFESESFDCVVHLAAQAGVRYSLKNPFAYVDSNLSGFVNLLECCRTSGIG
HHHHCCHHHHHHHCCCCHHEEEEHHHCCCEEECCCCCEEECCCHHHHHHHHHHHHHCCCC
HLVYASSSSVYGANTKAPFSVSDNVDHPVSLYAATKKANELMAHAYSHLYALPTTGLRFF
EEEEECCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHEECCCCCEEEE
TVYGPWGRPDMAYFKFVQAIEAGKPIDVYNHGHMQRDFTYIDDIVEGIVRLLPRVPTHAG
EEECCCCCCHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
AAPYRIYNIGNHQPVSLIEFIEVIEQALGKRAVKNLLPMQPGDVPATCADVDDLMREVGF
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCC
KPSTPLTVGIERFVCWYRDYLSAASPVVRTTTTVRPRWPAVAPVEVAG
CCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7961465 [H]