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The map label for this gene is glnA [H]

Identifier: 37520621

GI number: 37520621

Start: 1125236

End: 1126654

Strand: Direct

Name: glnA [H]

Synonym: gvip146

Alternate gene names: 37520621

Gene position: 1125236-1126654 (Clockwise)

Preceding gene: 37520619

Following gene: 37520623

Centisome position: 24.15

GC content: 59.62

Gene sequence:

>1419_bases
ATGGCGAAGACTCCCGATGACGTTCTCAAGATGATCCAGGATGAGGACGTCAAAATAGTTGACTTAAAATTCATCGATTT
GCCGGGCACCTGGCAGCATTGTTCGTTTGCCACCAACCAGATCGATGCGGATGCTTTTGCCGAGGGCATCCCCTTTGACG
GTTCGAGCATCCGTGGCTGGAAGGCCATCAACGAGTCGGACATGTTGATGGTGCCGGATCCGAGCACGGCGTTCATCGAT
CCGTTCTTCAAAGAGACGACTTTGAGCCTTACCTGCTCGATCCGCGAGCCGCGCACGGGCGAACCCTACGGCCGCGATCC
GCGCACGATTGCCGCGAAGGCACTTGAATATCTCAAAGGCACCGGCATCGCCGACACTGCCTACTTCGGTCCCGAGGCGG
AATTTTTCATCTTCGACGATGTGCGGTTCGATCAGACCCAGAATGCGGGCTACTACTATCTCGACTCGGTCGAAGGCAAC
TGGAACACCGGCCGCAACGAAGGTCCCAATCTAGGCTACAAGCCCCGCAACAAAGAAGGGTACTTCCCGGTTGCCCCCAC
CGATTCGATGCAGGATATCCGCACGGAGATGCTGCTCACCATGGGCAGTCTGGGGGTGCCCATCGAGAAGCACCACCACG
AAGTGGCCTCCGGCGGTCAGGCCGAACTTGGGGTGCGCTTTTTGTCGATGGTGGAGGCGGCGGACGCGCTGCAGATCTAC
AAGTACGTGATCAAAAACGTCGCCCGCAAGCACGGCAAGGTAGCGACCTTCATGCCCAAGCCAATCTTTAACGACAACGG
CACCGGCATGCACACCCACCAGTCGCTCTGGAAGGACGGCCAGCCGCTGTTCGCAGGCGACAAGTACGCCGACTTGAGCG
AAATGGCGCTCTACTACATCGGCGGCCTGCTCAAGCACGCCCCGGCGATTCTGGCCTTTTCCAACCCGACCACCAACTCC
TACAAGCGCCTGGTGCCCGGTTTCGAGGCGCCGGTGAACTTGGCCTACTCCCAGGGCAACCGCTCCGCTTCGATCCGCAT
CCCGCTGAGCGGCAAGAGCCCCAAGGCCAAGCGTCTGGAATTCCGCTGCCCCGACGCCAGCTCCAACGCCTACATCGCCT
TTGCGGCGATGCTGATGGCGGGTCTGGACGGCATCAAGAACAAGATCGACCCGGGCGAGCCTCTCGATGTCGATATTTAC
GAGCTGTCCGCCGAGGAGTTGGCCAAGGTGCCCTCCACCCCCGGTTCGCTCTCCGAAGCCCTCAAGGCGCTCGAAGAGGA
CCACGCTTTTCTGACCGAGGGCGGCGTCTTCAGCGAGGAGCTGATCAACGTCTGGATCGAGTACAAGCTCGAAAAAGAAG
TGATCCCGATGTCGCTCAGGCCCCACCCCTACGAGTTCTCGCTCTACTTCGACGTCTAA

Upstream 100 bases:

>100_bases
AAACACCTCCCCGACCCTCCTTAGTATGGGTTGGCCCTGCCCTGGCCGGCTTGGCTCCACTCTTTCACTTGGCCCACTAC
AGCTCTAGGAGAGCCTCGAC

Downstream 100 bases:

>100_bases
GCGACGCCTGAGCGGTAACTTTCGCCCCGGCGGCTACGGCTTGGCCGGGGTTTTGATTTTTCGGTTACCTTTGCTCCTCA
AACCACCTGCAAAGCAAATC

Product: glutamate--ammonia ligase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAKTPDDVLKMIQDEDVKIVDLKFIDLPGTWQHCSFATNQIDADAFAEGIPFDGSSIRGWKAINESDMLMVPDPSTAFID
PFFKETTLSLTCSIREPRTGEPYGRDPRTIAAKALEYLKGTGIADTAYFGPEAEFFIFDDVRFDQTQNAGYYYLDSVEGN
WNTGRNEGPNLGYKPRNKEGYFPVAPTDSMQDIRTEMLLTMGSLGVPIEKHHHEVASGGQAELGVRFLSMVEAADALQIY
KYVIKNVARKHGKVATFMPKPIFNDNGTGMHTHQSLWKDGQPLFAGDKYADLSEMALYYIGGLLKHAPAILAFSNPTTNS
YKRLVPGFEAPVNLAYSQGNRSASIRIPLSGKSPKAKRLEFRCPDASSNAYIAFAAMLMAGLDGIKNKIDPGEPLDVDIY
ELSAEELAKVPSTPGSLSEALKALEEDHAFLTEGGVFSEELINVWIEYKLEKEVIPMSLRPHPYEFSLYFDV

Sequences:

>Translated_472_residues
MAKTPDDVLKMIQDEDVKIVDLKFIDLPGTWQHCSFATNQIDADAFAEGIPFDGSSIRGWKAINESDMLMVPDPSTAFID
PFFKETTLSLTCSIREPRTGEPYGRDPRTIAAKALEYLKGTGIADTAYFGPEAEFFIFDDVRFDQTQNAGYYYLDSVEGN
WNTGRNEGPNLGYKPRNKEGYFPVAPTDSMQDIRTEMLLTMGSLGVPIEKHHHEVASGGQAELGVRFLSMVEAADALQIY
KYVIKNVARKHGKVATFMPKPIFNDNGTGMHTHQSLWKDGQPLFAGDKYADLSEMALYYIGGLLKHAPAILAFSNPTTNS
YKRLVPGFEAPVNLAYSQGNRSASIRIPLSGKSPKAKRLEFRCPDASSNAYIAFAAMLMAGLDGIKNKIDPGEPLDVDIY
ELSAEELAKVPSTPGSLSEALKALEEDHAFLTEGGVFSEELINVWIEYKLEKEVIPMSLRPHPYEFSLYFDV
>Mature_471_residues
AKTPDDVLKMIQDEDVKIVDLKFIDLPGTWQHCSFATNQIDADAFAEGIPFDGSSIRGWKAINESDMLMVPDPSTAFIDP
FFKETTLSLTCSIREPRTGEPYGRDPRTIAAKALEYLKGTGIADTAYFGPEAEFFIFDDVRFDQTQNAGYYYLDSVEGNW
NTGRNEGPNLGYKPRNKEGYFPVAPTDSMQDIRTEMLLTMGSLGVPIEKHHHEVASGGQAELGVRFLSMVEAADALQIYK
YVIKNVARKHGKVATFMPKPIFNDNGTGMHTHQSLWKDGQPLFAGDKYADLSEMALYYIGGLLKHAPAILAFSNPTTNSY
KRLVPGFEAPVNLAYSQGNRSASIRIPLSGKSPKAKRLEFRCPDASSNAYIAFAAMLMAGLDGIKNKIDPGEPLDVDIYE
LSAEELAKVPSTPGSLSEALKALEEDHAFLTEGGVFSEELINVWIEYKLEKEVIPMSLRPHPYEFSLYFDV

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=460, Percent_Identity=23.4782608695652, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1790301, Length=470, Percent_Identity=56.5957446808511, Blast_Score=548, Evalue=1e-157,
Organism=Escherichia coli, GI87081870, Length=291, Percent_Identity=26.4604810996564, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52350; Mature: 52219

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKTPDDVLKMIQDEDVKIVDLKFIDLPGTWQHCSFATNQIDADAFAEGIPFDGSSIRGW
CCCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCE
KAINESDMLMVPDPSTAFIDPFFKETTLSLTCSIREPRTGEPYGRDPRTIAAKALEYLKG
EECCCCCEEEECCCCCHHHCCHHCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
TGIADTAYFGPEAEFFIFDDVRFDQTQNAGYYYLDSVEGNWNTGRNEGPNLGYKPRNKEG
CCCCCCCCCCCCCCEEEEECEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
YFPVAPTDSMQDIRTEMLLTMGSLGVPIEKHHHEVASGGQAELGVRFLSMVEAADALQIY
EECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
KYVIKNVARKHGKVATFMPKPIFNDNGTGMHTHQSLWKDGQPLFAGDKYADLSEMALYYI
HHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
GGLLKHAPAILAFSNPTTNSYKRLVPGFEAPVNLAYSQGNRSASIRIPLSGKSPKAKRLE
HHHHHHCCEEEEECCCCCCHHHHHCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCEEE
FRCPDASSNAYIAFAAMLMAGLDGIKNKIDPGEPLDVDIYELSAEELAKVPSTPGSLSEA
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHCCCCCHHHHHH
LKALEEDHAFLTEGGVFSEELINVWIEYKLEKEVIPMSLRPHPYEFSLYFDV
HHHHHHCCCEEECCCCHHHHHHHHHHEEEECCCEECCCCCCCCEEEEEEEEC
>Mature Secondary Structure 
AKTPDDVLKMIQDEDVKIVDLKFIDLPGTWQHCSFATNQIDADAFAEGIPFDGSSIRGW
CCCHHHHHHHHCCCCEEEEEEEEECCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCE
KAINESDMLMVPDPSTAFIDPFFKETTLSLTCSIREPRTGEPYGRDPRTIAAKALEYLKG
EECCCCCEEEECCCCCHHHCCHHCCEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
TGIADTAYFGPEAEFFIFDDVRFDQTQNAGYYYLDSVEGNWNTGRNEGPNLGYKPRNKEG
CCCCCCCCCCCCCCEEEEECEEECCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCC
YFPVAPTDSMQDIRTEMLLTMGSLGVPIEKHHHEVASGGQAELGVRFLSMVEAADALQIY
EECCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
KYVIKNVARKHGKVATFMPKPIFNDNGTGMHTHQSLWKDGQPLFAGDKYADLSEMALYYI
HHHHHHHHHHCCCEEEECCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHH
GGLLKHAPAILAFSNPTTNSYKRLVPGFEAPVNLAYSQGNRSASIRIPLSGKSPKAKRLE
HHHHHHCCEEEEECCCCCCHHHHHCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCEEE
FRCPDASSNAYIAFAAMLMAGLDGIKNKIDPGEPLDVDIYELSAEELAKVPSTPGSLSEA
EECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHCCCCCHHHHHH
LKALEEDHAFLTEGGVFSEELINVWIEYKLEKEVIPMSLRPHPYEFSLYFDV
HHHHHHCCCEEECCCCHHHHHHHHHHEEEECCCEECCCCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7727755; 8905231 [H]