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The map label for this gene is lipA1

Identifier: 37520598

GI number: 37520598

Start: 1104981

End: 1105877

Strand: Reverse

Name: lipA1

Synonym: gll1029

Alternate gene names: 37520598

Gene position: 1105877-1104981 (Counterclockwise)

Preceding gene: 37520599

Following gene: 37520597

Centisome position: 23.74

GC content: 62.32

Gene sequence:

>897_bases
ATGGTCACCAAGCCGGACTGGCTCAGGGTCAAAGCGCCGCAGCGCGAGCGGGTGGGAGCAGTTAAAGACATCCTGCGCGA
CCTGGCGCTCAACACCGTCTGTGAGGAAGCTTCTTGCCCAAATATCGGCGAATGTTTCAAAGCCGGGACGGCAACGTTTC
TGATCATGGGACCGGCCTGCACGCGGGCTTGCCCTTACTGCGACATCGATTTTGAGAAATATCCCAAGGCCCTCGATCCG
ACCGAACCCGAGCGGCTCGCACAGGCCGTGCGGCGGATGGGCCTGCGCCATGTGGTGATTACCTCGGTCAACCGCGACGA
TCTGGCCGACGGCGGCGCTCTGCAGTTTGCCCGCTGCATCGAAGCGGTGCGCCGGGTCATGCCCCAAACGACCATCGAAG
TGTTGATCCCCGATTTTTGCGGCTCCGAAGCGGCCCTCGATATCGTGATTGCCGCCCATCCACAAGTAATCAATCACAAT
ACCGAGACCGTCCCTCGGCTCTACCGGCGGGTCAGACCCCAGGGCGACTACGGACGCACTTTGCAATTGCTCGAGCGCGT
TCGCGCCAAAGCATCCCACATCTACACCAAGTCGGGCCTGATGGCGGGCCTTGGCGAGAGCGAAGCGGAAGTGCTCGCGG
TCATGGCAGATCTGCGGGCCGTCTACTGCGACATCCTGACCATCGGCCAGTACCTGCAGCCCACCCCCAAACACCTCAAA
GTCGAAGCCTTTGTCGAACCGGCGCTTTTTGAGCGCTGGCGGCGGGCGGGCGAAGGGATGGGCTTCTTGCAGATGGTCTC
CTCGCCCCTGACGCGCAGTTCCTACCACGCCGAGCAGGTGCAGCGACTGATGCGCTCCCACCCCCGGACCCCCAAAAATC
AGCACTCGCCGGAGTAA

Upstream 100 bases:

>100_bases
ACAAATCAGGTCAGACTGAGCGGGCATACTTCCAGCCCCGACCCAATTGGGTAGCCGTTGGTCTGTGCGTTACACTCGCA
GATGTCGTCCGCGAATTTTT

Downstream 100 bases:

>100_bases
AGGGTTAGCACTCTCGGGTCTTCAGTGCTAAACTCTGTGCTTGGAAGGGAGTCGTTCCTTCCACAAAGACTTTAGTGAGC
TGTAAGAGAGGTTTGCATGG

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 1; Lipoate synthase 1; Lipoic acid synthase 1; Sulfur insertion protein lipA1

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MVTKPDWLRVKAPQRERVGAVKDILRDLALNTVCEEASCPNIGECFKAGTATFLIMGPACTRACPYCDIDFEKYPKALDP
TEPERLAQAVRRMGLRHVVITSVNRDDLADGGALQFARCIEAVRRVMPQTTIEVLIPDFCGSEAALDIVIAAHPQVINHN
TETVPRLYRRVRPQGDYGRTLQLLERVRAKASHIYTKSGLMAGLGESEAEVLAVMADLRAVYCDILTIGQYLQPTPKHLK
VEAFVEPALFERWRRAGEGMGFLQMVSSPLTRSSYHAEQVQRLMRSHPRTPKNQHSPE

Sequences:

>Translated_298_residues
MVTKPDWLRVKAPQRERVGAVKDILRDLALNTVCEEASCPNIGECFKAGTATFLIMGPACTRACPYCDIDFEKYPKALDP
TEPERLAQAVRRMGLRHVVITSVNRDDLADGGALQFARCIEAVRRVMPQTTIEVLIPDFCGSEAALDIVIAAHPQVINHN
TETVPRLYRRVRPQGDYGRTLQLLERVRAKASHIYTKSGLMAGLGESEAEVLAVMADLRAVYCDILTIGQYLQPTPKHLK
VEAFVEPALFERWRRAGEGMGFLQMVSSPLTRSSYHAEQVQRLMRSHPRTPKNQHSPE
>Mature_298_residues
MVTKPDWLRVKAPQRERVGAVKDILRDLALNTVCEEASCPNIGECFKAGTATFLIMGPACTRACPYCDIDFEKYPKALDP
TEPERLAQAVRRMGLRHVVITSVNRDDLADGGALQFARCIEAVRRVMPQTTIEVLIPDFCGSEAALDIVIAAHPQVINHN
TETVPRLYRRVRPQGDYGRTLQLLERVRAKASHIYTKSGLMAGLGESEAEVLAVMADLRAVYCDILTIGQYLQPTPKHLK
VEAFVEPALFERWRRAGEGMGFLQMVSSPLTRSSYHAEQVQRLMRSHPRTPKNQHSPE

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=279, Percent_Identity=41.5770609318996, Blast_Score=239, Evalue=2e-63,
Organism=Homo sapiens, GI37577164, Length=244, Percent_Identity=41.3934426229508, Blast_Score=205, Evalue=4e-53,
Organism=Escherichia coli, GI1786846, Length=280, Percent_Identity=50.7142857142857, Blast_Score=287, Evalue=5e-79,
Organism=Caenorhabditis elegans, GI32564533, Length=282, Percent_Identity=40.4255319148936, Blast_Score=217, Evalue=6e-57,
Organism=Saccharomyces cerevisiae, GI6324770, Length=279, Percent_Identity=41.2186379928315, Blast_Score=238, Evalue=6e-64,
Organism=Drosophila melanogaster, GI221513272, Length=279, Percent_Identity=40.8602150537634, Blast_Score=223, Evalue=8e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA1_GLOVI (Q7NLU2)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923975.1
- ProteinModelPortal:   Q7NLU2
- GeneID:   2599147
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll1029
- NMPDR:   fig|251221.1.peg.1029
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BioCyc:   GVIO251221:GLL1029-MONOMER
- BRENDA:   2.8.1.8
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 33363; Mature: 33363

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTKPDWLRVKAPQRERVGAVKDILRDLALNTVCEEASCPNIGECFKAGTATFLIMGPAC
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCHH
TRACPYCDIDFEKYPKALDPTEPERLAQAVRRMGLRHVVITSVNRDDLADGGALQFARCI
HCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCHHHCCCCCHHHHHHHH
EAVRRVMPQTTIEVLIPDFCGSEAALDIVIAAHPQVINHNTETVPRLYRRVRPQGDYGRT
HHHHHHCCHHHHHHHCCCCCCCCHHEEEEEECCCHHHCCCCHHHHHHHHHHCCCCCCHHH
LQLLERVRAKASHIYTKSGLMAGLGESEAEVLAVMADLRAVYCDILTIGQYLQPTPKHLK
HHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
VEAFVEPALFERWRRAGEGMGFLQMVSSPLTRSSYHAEQVQRLMRSHPRTPKNQHSPE
HHHHHCHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MVTKPDWLRVKAPQRERVGAVKDILRDLALNTVCEEASCPNIGECFKAGTATFLIMGPAC
CCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEEECCHH
TRACPYCDIDFEKYPKALDPTEPERLAQAVRRMGLRHVVITSVNRDDLADGGALQFARCI
HCCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCHHHCCCCCHHHHHHHH
EAVRRVMPQTTIEVLIPDFCGSEAALDIVIAAHPQVINHNTETVPRLYRRVRPQGDYGRT
HHHHHHCCHHHHHHHCCCCCCCCHHEEEEEECCCHHHCCCCHHHHHHHHHHCCCCCCHHH
LQLLERVRAKASHIYTKSGLMAGLGESEAEVLAVMADLRAVYCDILTIGQYLQPTPKHLK
HHHHHHHHHHHHHHHHHCCHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEE
VEAFVEPALFERWRRAGEGMGFLQMVSSPLTRSSYHAEQVQRLMRSHPRTPKNQHSPE
HHHHHCHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292