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The map label for this gene is gutB [H]

Identifier: 37520509

GI number: 37520509

Start: 996963

End: 998045

Strand: Direct

Name: gutB [H]

Synonym: glr0940

Alternate gene names: 37520509

Gene position: 996963-998045 (Clockwise)

Preceding gene: 37520508

Following gene: 37520511

Centisome position: 21.4

GC content: 64.64

Gene sequence:

>1083_bases
GTGGATGGCAACAAGCCCATGGACGTTCCCCAGCTGATGCACGCGGCGGTTTTCTACGGCGTAGGCGATCTGCGCTACGA
ACAGGTGCCGGTTCCCGAAGTCGCGGCGGACGAGGTGCTGGTGCAGGTGCGCGCCTGCGGTCTGTGCCAGTCGGACATCA
AAAAGCTGCGCTACCCGCTACTCGACCCGCCGCGCATCTTCGGCCACGAGACGAGCGGCACGATCGTGCGCGCCGGGGAA
GCGGTGGAGCACTGGCGGGTAGGCGAGCGGGTGGTGGTGCTGCACCATATTCCTTGTTTTCACTGCCCCTACTGCCTTAA
CGAGAACTACTCGATGTGCCGGGTCTACAAAGAAGTGACCACTACCGCCGGCTTTGCACCCGCCGGCGGCGGCTTCGCCG
AGTACGTCAAGGTGCCCGGCCACATCGTGCGCTCGGGAGGTCTGATGGCGATTCCCGACGGGGTGAGCTTCGAAGAAGCG
AGCTTCGTCGAACCCACCAACTGCTGCTTGAAGGCCGTGAAAAAAGCCCAGGTCCAGCCCGGCCAGTGGGTGCTGATCAC
CGGCGCCGGTCCCATCGGGCTGATGTTCGCCATGCTCTGCCGCCAGTTCGGAGCGAAACCGATCGTCACCGACTTGCTCG
CCTCGCGCGTCGAGCGCGCCCGCGCGCTCGGGGCCGAGGCTGCCCTCGATGCGCGCTCCGCCGATCTGACCGCCCGGATC
CACGCCCTCACCGGCGGCCTCGGAGTGGATGTCGCCTTGCTCGCGGTGCCCAGCGTGCAGGCCTTTTCCCAGGCCCTCGA
TGCGACGCGCAAGGGCGGCAAGATTCTGTTTTTTGCCGAATTTGCCGAATCGGTGCAAATCCCGCTCGATCCGAACGTGC
TCTACCGGCGCGAAATCGACTTGATGGGCTCCTACAGCTCCTCGTTCAAAGTCCAGCACCTGGCAGCCGATCTTGTCTTC
GGGCGGCGCATCGACGTGCAGGCGCTCATCTCGGACTTTTATCCGCTCGCTGAACTGGCGGCGGCGGTGGAGCAGGCCGT
GCATCCAACCCCCGAGACCTGCAAAATTCTTATCCGGCCTTGA

Upstream 100 bases:

>100_bases
CCGATTAGGGCAATGGTCGCGAAATCGCTGAGTAACCTATCTGTAGTATGCTCCTGCGCCTTGCCCGCAGGGCAAAAGCG
GCCAAAAATGGTGCTGGCGA

Downstream 100 bases:

>100_bases
CCTGCCGTCCCCCCGGCAGGAGCCAGGCGATGCCGCCCAATAGAGCGGCGACGGTGTAGACGGCGAACCACAGCGCTCCA
AAACCAAGGGCCTGCTCCGC

Product: sorbitol dehydrogenase

Products: NA

Alternate protein names: Glucitol dehydrogenase; L-iditol 2-dehydrogenase [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MDGNKPMDVPQLMHAAVFYGVGDLRYEQVPVPEVAADEVLVQVRACGLCQSDIKKLRYPLLDPPRIFGHETSGTIVRAGE
AVEHWRVGERVVVLHHIPCFHCPYCLNENYSMCRVYKEVTTTAGFAPAGGGFAEYVKVPGHIVRSGGLMAIPDGVSFEEA
SFVEPTNCCLKAVKKAQVQPGQWVLITGAGPIGLMFAMLCRQFGAKPIVTDLLASRVERARALGAEAALDARSADLTARI
HALTGGLGVDVALLAVPSVQAFSQALDATRKGGKILFFAEFAESVQIPLDPNVLYRREIDLMGSYSSSFKVQHLAADLVF
GRRIDVQALISDFYPLAELAAAVEQAVHPTPETCKILIRP

Sequences:

>Translated_360_residues
MDGNKPMDVPQLMHAAVFYGVGDLRYEQVPVPEVAADEVLVQVRACGLCQSDIKKLRYPLLDPPRIFGHETSGTIVRAGE
AVEHWRVGERVVVLHHIPCFHCPYCLNENYSMCRVYKEVTTTAGFAPAGGGFAEYVKVPGHIVRSGGLMAIPDGVSFEEA
SFVEPTNCCLKAVKKAQVQPGQWVLITGAGPIGLMFAMLCRQFGAKPIVTDLLASRVERARALGAEAALDARSADLTARI
HALTGGLGVDVALLAVPSVQAFSQALDATRKGGKILFFAEFAESVQIPLDPNVLYRREIDLMGSYSSSFKVQHLAADLVF
GRRIDVQALISDFYPLAELAAAVEQAVHPTPETCKILIRP
>Mature_360_residues
MDGNKPMDVPQLMHAAVFYGVGDLRYEQVPVPEVAADEVLVQVRACGLCQSDIKKLRYPLLDPPRIFGHETSGTIVRAGE
AVEHWRVGERVVVLHHIPCFHCPYCLNENYSMCRVYKEVTTTAGFAPAGGGFAEYVKVPGHIVRSGGLMAIPDGVSFEEA
SFVEPTNCCLKAVKKAQVQPGQWVLITGAGPIGLMFAMLCRQFGAKPIVTDLLASRVERARALGAEAALDARSADLTARI
HALTGGLGVDVALLAVPSVQAFSQALDATRKGGKILFFAEFAESVQIPLDPNVLYRREIDLMGSYSSSFKVQHLAADLVF
GRRIDVQALISDFYPLAELAAAVEQAVHPTPETCKILIRP

Specific function: Reduces glucitol to fructose [H]

COG id: COG1063

COG function: function code ER; Threonine dehydrogenase and related Zn-dependent dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI156627571, Length=342, Percent_Identity=28.0701754385965, Blast_Score=139, Evalue=3e-33,
Organism=Homo sapiens, GI156523966, Length=357, Percent_Identity=25.7703081232493, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI4501939, Length=357, Percent_Identity=25.7703081232493, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI71565154, Length=283, Percent_Identity=27.5618374558304, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI262073058, Length=342, Percent_Identity=21.9298245614035, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI71743840, Length=342, Percent_Identity=21.9298245614035, Blast_Score=74, Evalue=3e-13,
Organism=Escherichia coli, GI1788073, Length=344, Percent_Identity=28.1976744186047, Blast_Score=139, Evalue=4e-34,
Organism=Escherichia coli, GI226510992, Length=339, Percent_Identity=28.3185840707965, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1788075, Length=340, Percent_Identity=26.7647058823529, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1790045, Length=255, Percent_Identity=30.9803921568627, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI87082125, Length=315, Percent_Identity=29.2063492063492, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788407, Length=347, Percent_Identity=26.8011527377522, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1787863, Length=214, Percent_Identity=34.5794392523364, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081918, Length=271, Percent_Identity=29.520295202952, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1786825, Length=312, Percent_Identity=30.4487179487179, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790718, Length=311, Percent_Identity=24.7588424437299, Blast_Score=88, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17562876, Length=341, Percent_Identity=30.2052785923754, Blast_Score=164, Evalue=9e-41,
Organism=Caenorhabditis elegans, GI17562878, Length=341, Percent_Identity=29.6187683284457, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17562584, Length=339, Percent_Identity=28.9085545722714, Blast_Score=94, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI71988145, Length=342, Percent_Identity=26.6081871345029, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17562582, Length=313, Percent_Identity=28.4345047923323, Blast_Score=87, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6319955, Length=357, Percent_Identity=27.4509803921569, Blast_Score=135, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6322619, Length=357, Percent_Identity=27.4509803921569, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6323099, Length=349, Percent_Identity=26.0744985673352, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319258, Length=241, Percent_Identity=32.7800829875519, Blast_Score=102, Evalue=9e-23,
Organism=Saccharomyces cerevisiae, GI6319621, Length=249, Percent_Identity=33.3333333333333, Blast_Score=92, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6324486, Length=251, Percent_Identity=27.8884462151394, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6319257, Length=240, Percent_Identity=28.75, Blast_Score=90, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323961, Length=275, Percent_Identity=27.6363636363636, Blast_Score=87, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6323729, Length=247, Percent_Identity=25.5060728744939, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6319520, Length=265, Percent_Identity=24.5283018867925, Blast_Score=81, Evalue=2e-16,
Organism=Drosophila melanogaster, GI17737897, Length=354, Percent_Identity=32.4858757062147, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI17137530, Length=354, Percent_Identity=31.638418079096, Blast_Score=149, Evalue=2e-36,
Organism=Drosophila melanogaster, GI17737895, Length=280, Percent_Identity=25.3571428571429, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR002328
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: =1.1.1.14 [H]

Molecular weight: Translated: 38999; Mature: 38999

Theoretical pI: Translated: 6.66; Mature: 6.66

Prosite motif: PS00059 ADH_ZINC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDGNKPMDVPQLMHAAVFYGVGDLRYEQVPVPEVAADEVLVQVRACGLCQSDIKKLRYPL
CCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCC
LDPPRIFGHETSGTIVRAGEAVEHWRVGERVVVLHHIPCFHCPYCLNENYSMCRVYKEVT
CCCCHHCCCCCCCCEEECCCHHHHHHCCCEEEEEECCCHHCCCHHHCCCHHHHHHHHHHH
TTAGFAPAGGGFAEYVKVPGHIVRSGGLMAIPDGVSFEEASFVEPTNCCLKAVKKAQVQP
HHCCCCCCCCCHHHHHHCCCCEEECCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
GQWVLITGAGPIGLMFAMLCRQFGAKPIVTDLLASRVERARALGAEAALDARSADLTARI
CCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHEEE
HALTGGLGVDVALLAVPSVQAFSQALDATRKGGKILFFAEFAESVQIPLDPNVLYRREID
EHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEECCCCCCEEEEEEHH
LMGSYSSSFKVQHLAADLVFGRRIDVQALISDFYPLAELAAAVEQAVHPTPETCKILIRP
HHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEECC
>Mature Secondary Structure
MDGNKPMDVPQLMHAAVFYGVGDLRYEQVPVPEVAADEVLVQVRACGLCQSDIKKLRYPL
CCCCCCCCHHHHHHHHHHHCCCCCEECCCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCC
LDPPRIFGHETSGTIVRAGEAVEHWRVGERVVVLHHIPCFHCPYCLNENYSMCRVYKEVT
CCCCHHCCCCCCCCEEECCCHHHHHHCCCEEEEEECCCHHCCCHHHCCCHHHHHHHHHHH
TTAGFAPAGGGFAEYVKVPGHIVRSGGLMAIPDGVSFEEASFVEPTNCCLKAVKKAQVQP
HHCCCCCCCCCHHHHHHCCCCEEECCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
GQWVLITGAGPIGLMFAMLCRQFGAKPIVTDLLASRVERARALGAEAALDARSADLTARI
CCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHCCCCCCHHEEE
HALTGGLGVDVALLAVPSVQAFSQALDATRKGGKILFFAEFAESVQIPLDPNVLYRREID
EHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEECCCCCCEEEEEEHH
LMGSYSSSFKVQHLAADLVFGRRIDVQALISDFYPLAELAAAVEQAVHPTPETCKILIRP
HHCCCCCCCCHHHHHHHHHHCCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1460002; 9384377; 8195086 [H]