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The map label for this gene is mutY [H]

Identifier: 37520435

GI number: 37520435

Start: 912700

End: 913827

Strand: Reverse

Name: mutY [H]

Synonym: gll0866

Alternate gene names: 37520435

Gene position: 913827-912700 (Counterclockwise)

Preceding gene: 37520442

Following gene: 37520434

Centisome position: 19.61

GC content: 61.52

Gene sequence:

>1128_bases
GTGGGATACAGCAGTGCAAAACCATCGCTCAACAACTGCTCCGAGCCCGAACAGGTCGTCCGGCTGCGGGCCCAACTGTT
GGAATGGTACGGCCGCATGGGTCGAGATTTGCCCTGGCGCCGTACCCGTGATCCTTATGCCATCTGGATTTCGGAGATCA
TGCTTCAGCAGACCCAGGTGAAAACGGTCCTGCCCTACTACCAAAGGTGGCTTGCGGCTCTGCCGACCGTCGCGGCTCTT
GCCGCCGCCGAGCTGGAGGCGGTGCTTAAGCTCTGGGAGGGCCTGGGCTACTACACCCGGGCGCGCAACCTCCATAAAGC
CGCGCAGGTGATTGTCAAAGAGCACGGCGGAGTTTTTCCCGAGACCGCCCAGCAGTTGCAACAAGCGCTGCCGGGTATCG
GCCGCTCGACGGCAGGTGCAATCGCAAGCAGTGCCTTCGGCCGGTGTGAAGCAATCCTCGATGCCAACGCCCGCAGGGTA
CTGGGGCGCCTGTTCGCGGTGGGCGATCCTCCTGCGCGGGCAGAAGCGAAGCTTTGGGAGATCTCACAGCGGCTGGTAGA
CCCGCAAGCTCCCCACAACTTCAATCAGGCGTTGATGGATCTAGGAGCGACAGTTTGTACAGCAAGATCTCCTCTGTGTC
TGCTCTGTCCGTGGCAAGTCGATTGTCTGGGGCGCCGCTCGGGAGATCCCACGCACTTTCCGGTTCGCCCCGCCCGCGCG
GTGCGCTCCGAGATCGCAGGGGTCAGCGTCGCGATCGAGTGCCAGGGAAAATTCTTACTTGTCCGCCGACCGGAGCGGGG
ATTGCTCGCTGGGCTCTGGGAATTTCCGTTTGTCGAATCAGTTGGCGGCGGCGAGCCGGAAGAAACCGTCCGAGTGGCCT
TTGGCAATCGCCTGGAATCGCTTGAGCGGCTGGGGCAGGTGGAGCACGAATTTACCCACCGCCATCTGACAGCCCAAGTA
CTGCGGGCACAGTGGATTGCCGCTCCGGCTGCGCTGCCGAAGGTTTTTGACTGCCGTGAACACACCTGGCAGCCCCCCGA
GTGCTGGCTTAAATTCCCGATGCCGGGTTATGTTCACAAGATCTGCAAGCTTTTGAAAGAGGCACTCCCGAAAGTCTCCG
TCGACTGA

Upstream 100 bases:

>100_bases
AGTGCGGAAGTTTCGCCTCTGTTGTGCTGCAGCGTTTGTTTTATTGGCCGCGGGGGGACGATGCTAGCCTGTCAAAGGTC
TTTGAAGGGACGGGCAAGTT

Downstream 100 bases:

>100_bases
GTATGCCAGCAGACGCACCCCACCACAGGAGGGGGAGTATCCTGAAACGGGCAGCGGCAAACTGTGCTTTTTGAAACGTT
TGAGCGAAGCATACTGAACG

Product: A/G-specific adenine glycosylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MGYSSAKPSLNNCSEPEQVVRLRAQLLEWYGRMGRDLPWRRTRDPYAIWISEIMLQQTQVKTVLPYYQRWLAALPTVAAL
AAAELEAVLKLWEGLGYYTRARNLHKAAQVIVKEHGGVFPETAQQLQQALPGIGRSTAGAIASSAFGRCEAILDANARRV
LGRLFAVGDPPARAEAKLWEISQRLVDPQAPHNFNQALMDLGATVCTARSPLCLLCPWQVDCLGRRSGDPTHFPVRPARA
VRSEIAGVSVAIECQGKFLLVRRPERGLLAGLWEFPFVESVGGGEPEETVRVAFGNRLESLERLGQVEHEFTHRHLTAQV
LRAQWIAAPAALPKVFDCREHTWQPPECWLKFPMPGYVHKICKLLKEALPKVSVD

Sequences:

>Translated_375_residues
MGYSSAKPSLNNCSEPEQVVRLRAQLLEWYGRMGRDLPWRRTRDPYAIWISEIMLQQTQVKTVLPYYQRWLAALPTVAAL
AAAELEAVLKLWEGLGYYTRARNLHKAAQVIVKEHGGVFPETAQQLQQALPGIGRSTAGAIASSAFGRCEAILDANARRV
LGRLFAVGDPPARAEAKLWEISQRLVDPQAPHNFNQALMDLGATVCTARSPLCLLCPWQVDCLGRRSGDPTHFPVRPARA
VRSEIAGVSVAIECQGKFLLVRRPERGLLAGLWEFPFVESVGGGEPEETVRVAFGNRLESLERLGQVEHEFTHRHLTAQV
LRAQWIAAPAALPKVFDCREHTWQPPECWLKFPMPGYVHKICKLLKEALPKVSVD
>Mature_374_residues
GYSSAKPSLNNCSEPEQVVRLRAQLLEWYGRMGRDLPWRRTRDPYAIWISEIMLQQTQVKTVLPYYQRWLAALPTVAALA
AAELEAVLKLWEGLGYYTRARNLHKAAQVIVKEHGGVFPETAQQLQQALPGIGRSTAGAIASSAFGRCEAILDANARRVL
GRLFAVGDPPARAEAKLWEISQRLVDPQAPHNFNQALMDLGATVCTARSPLCLLCPWQVDCLGRRSGDPTHFPVRPARAV
RSEIAGVSVAIECQGKFLLVRRPERGLLAGLWEFPFVESVGGGEPEETVRVAFGNRLESLERLGQVEHEFTHRHLTAQVL
RAQWIAAPAALPKVFDCREHTWQPPECWLKFPMPGYVHKICKLLKEALPKVSVD

Specific function: Adenine glycosylase active on G-A mispairs. MutY also corrects error-prone DNA synthesis past GO lesions which are due to the oxidatively damaged form of guanine:7,8-dihydro-8- oxoguanine (8-oxo-dGTP) [H]

COG id: COG1194

COG function: function code L; A/G-specific DNA glycosylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI6912520, Length=368, Percent_Identity=41.5760869565217, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI115298648, Length=368, Percent_Identity=41.5760869565217, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI115298654, Length=368, Percent_Identity=41.5760869565217, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI115298652, Length=368, Percent_Identity=41.5760869565217, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI115298650, Length=368, Percent_Identity=41.5760869565217, Blast_Score=246, Evalue=2e-65,
Organism=Homo sapiens, GI190358497, Length=368, Percent_Identity=41.5760869565217, Blast_Score=246, Evalue=2e-65,
Organism=Escherichia coli, GI1789331, Length=306, Percent_Identity=44.1176470588235, Blast_Score=249, Evalue=2e-67,
Organism=Escherichia coli, GI1787920, Length=213, Percent_Identity=26.7605633802817, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170
- InterPro:   IPR005760
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF10576 EndIII_4Fe-2S; PF00633 HHH; PF00730 HhH-GPD [H]

EC number: 3.2.2.-

Molecular weight: Translated: 41863; Mature: 41732

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGYSSAKPSLNNCSEPEQVVRLRAQLLEWYGRMGRDLPWRRTRDPYAIWISEIMLQQTQV
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
KTVLPYYQRWLAALPTVAALAAAELEAVLKLWEGLGYYTRARNLHKAAQVIVKEHGGVFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCH
ETAQQLQQALPGIGRSTAGAIASSAFGRCEAILDANARRVLGRLFAVGDPPARAEAKLWE
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHH
ISQRLVDPQAPHNFNQALMDLGATVCTARSPLCLLCPWQVDCLGRRSGDPTHFPVRPARA
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCCCCCCCCCCHHHH
VRSEIAGVSVAIECQGKFLLVRRPERGLLAGLWEFPFVESVGGGEPEETVRVAFGNRLES
HHHHHCCCEEEEEECCCEEEEECCCCCHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHH
LERLGQVEHEFTHRHLTAQVLRAQWIAAPAALPKVFDCREHTWQPPECWLKFPMPGYVHK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCHHHHH
ICKLLKEALPKVSVD
HHHHHHHHCCCCCCC
>Mature Secondary Structure 
GYSSAKPSLNNCSEPEQVVRLRAQLLEWYGRMGRDLPWRRTRDPYAIWISEIMLQQTQV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHH
KTVLPYYQRWLAALPTVAALAAAELEAVLKLWEGLGYYTRARNLHKAAQVIVKEHGGVFP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCH
ETAQQLQQALPGIGRSTAGAIASSAFGRCEAILDANARRVLGRLFAVGDPPARAEAKLWE
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHH
ISQRLVDPQAPHNFNQALMDLGATVCTARSPLCLLCPWQVDCLGRRSGDPTHFPVRPARA
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCCCHHHHCCCCCCCCCCCCCHHHH
VRSEIAGVSVAIECQGKFLLVRRPERGLLAGLWEFPFVESVGGGEPEETVRVAFGNRLES
HHHHHCCCEEEEEECCCEEEEECCCCCHHHHHHHCCCHHHCCCCCHHHHHHHHHHHHHHH
LERLGQVEHEFTHRHLTAQVLRAQWIAAPAALPKVFDCREHTWQPPECWLKFPMPGYVHK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHEEECCCCHHHHH
ICKLLKEALPKVSVD
HHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: 4Fe-4S Cluster [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Glycosylases; Hydrolysing N-glycosyl compounds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2197596; 2001994; 9278503; 9846876 [H]