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The map label for this gene is phrA

Identifier: 37520404

GI number: 37520404

Start: 890421

End: 891923

Strand: Direct

Name: phrA

Synonym: gvip101

Alternate gene names: 37520404

Gene position: 890421-891923 (Clockwise)

Preceding gene: 37520403

Following gene: 37520405

Centisome position: 19.11

GC content: 60.68

Gene sequence:

>1503_bases
ATGTCTACCAAAACTGTCCTTGTCTGGTACCGCAACGACCTGCGCGTGCACGACCACGAGCCGCTCACCAGCGCGCTGCA
CAAAAATGCCAGGGTCGTGGCGCTGTATTGTTTCGATCCGCGGCAGTTCGGCAAAGCCCCCTTCGGTTTTGAGAAGACCG
GGCCTTTTCGCGCCCGGTTTTTGCTCGAAAGTGTCGCGGATCTGCGCCGTTCGCTGAGGCAACTGGGAAGCGACCTGCTG
GTGCGCCGGGGCCATCCCGAGGAGGTGATCCCAGCACTGGTAAGCGAGTTGGAGATTGCGGCCGTCCACTACCACGGCGA
GGTCACTTCTGAAGAACTCGTGGTCGAGCGGGATCTTCAGGCAGCGCTCGCGCCTTTGAACGTGCCGGTGCGTTCGTTCT
GGGGAACAACCCTGGTGCACCCGGATGACTTGCCCTTCGCTATCGAGGCGATCCCTGAACTATTTACGGATTTTCGCAAG
CAGGTCGAGCGCTCCGCCGCCATCAACCCGCCCCTGCCCGCCCCTGCGAAGCTGCCGCCCTTGCCTGCAGTCGATCCCGG
CGAGATCCCGCAACTGGCGGATTTGGGTCTTGAAAGCCCGGTCACAGACGAACGGGCTGTGCTGCAATTCAAAGGCGGCG
AGACATCGGGGCTCGCTCGCCTCGAAGAATACTTTTGGCAAAAAAGTTTGCTCAAGTCCTACAAACAGACCCGCAATGGC
ATGCTCGGGGCCGATTATTCTTCAAAGTTTTCAGCCTGGCTCGCCCTGGGGTGCCTCTCCGCCCGCTATATCCACGAGCA
GGTGCAGACCTACGAGACAAAGCGCATCAAGAACGATTCGACCTACTGGCTTATTTTCGAACTGCTTTGGCGCGATTACT
TTCGCTTTATCGCCGCCAAGCACGGGGATCGGTTGTTTTACACTGCGGGCCTTCGGGGACTGGACATTCCCTGGAAAGAG
GACTGGGAGCGCTTTGAACTGTGGCGGACGGGCCAAACCGGCTTTCCGCTGGTCGATGCCAACATGCGCGAACTGGCGGC
TACCGGCTTTATGTCCAACCGGGGCCGACAGAACGTCGCGAGCTTTTTGACCAAGAACCTGGGCATCCACTGGCACATGG
GAGCCGAGTGGTTCGAGTCGCGGCTGATCGATTACGACGTGGCGAGCAACTGGGGCAACTGGAACTACACCGCCGGTGTC
GGCAACGACGCGCGCGGCTTTCGCTTCTTCAACATCCTCAAGCAGGCCAGAGACTACGACCCCGACGGCGCCTACGTCAA
ACACTGGCTGCCGGAACTGGCCGGATTGCCCCCGGCCAGGGTGCACGAGCCCTGGAAATTGCTGCCCGTCGAGCAGAAGC
GCTTCGGGGTCAGACTGGGTGTCGACTACCCGCAACCGGTGGTCGATCTGTTCCAATCGGCCGCCGCCAACGAGGCGATT
TACAACGCTGCCTGGGAGCACCACCACAGAAAGCGCCGCGGACAACCGCGAAGCCGCACTTGA

Upstream 100 bases:

>100_bases
CCTGGCCCGGCAGGGTACAGCTGTCTCGACGGTGGTGCTGGTCGAGCGCTAGGGCGGCGGTTGTTGCGTATCATGGCAGG
CAGGGTATCGATGTGCACCG

Downstream 100 bases:

>100_bases
GTCGATATATAGGAGCGGTTAAAGTACGTGTAGTGTCAAAGCACCACCTGCGACTATGGACTGGCTGCAAGTTCCGCCTC
TGCTGGAAAAAGATCCCGAA

Product: cryptochrome

Products: 2 pyrimidine residues (in DNA) [C]

Alternate protein names: NA

Number of amino acids: Translated: 500; Mature: 499

Protein sequence:

>500_residues
MSTKTVLVWYRNDLRVHDHEPLTSALHKNARVVALYCFDPRQFGKAPFGFEKTGPFRARFLLESVADLRRSLRQLGSDLL
VRRGHPEEVIPALVSELEIAAVHYHGEVTSEELVVERDLQAALAPLNVPVRSFWGTTLVHPDDLPFAIEAIPELFTDFRK
QVERSAAINPPLPAPAKLPPLPAVDPGEIPQLADLGLESPVTDERAVLQFKGGETSGLARLEEYFWQKSLLKSYKQTRNG
MLGADYSSKFSAWLALGCLSARYIHEQVQTYETKRIKNDSTYWLIFELLWRDYFRFIAAKHGDRLFYTAGLRGLDIPWKE
DWERFELWRTGQTGFPLVDANMRELAATGFMSNRGRQNVASFLTKNLGIHWHMGAEWFESRLIDYDVASNWGNWNYTAGV
GNDARGFRFFNILKQARDYDPDGAYVKHWLPELAGLPPARVHEPWKLLPVEQKRFGVRLGVDYPQPVVDLFQSAAANEAI
YNAAWEHHHRKRRGQPRSRT

Sequences:

>Translated_500_residues
MSTKTVLVWYRNDLRVHDHEPLTSALHKNARVVALYCFDPRQFGKAPFGFEKTGPFRARFLLESVADLRRSLRQLGSDLL
VRRGHPEEVIPALVSELEIAAVHYHGEVTSEELVVERDLQAALAPLNVPVRSFWGTTLVHPDDLPFAIEAIPELFTDFRK
QVERSAAINPPLPAPAKLPPLPAVDPGEIPQLADLGLESPVTDERAVLQFKGGETSGLARLEEYFWQKSLLKSYKQTRNG
MLGADYSSKFSAWLALGCLSARYIHEQVQTYETKRIKNDSTYWLIFELLWRDYFRFIAAKHGDRLFYTAGLRGLDIPWKE
DWERFELWRTGQTGFPLVDANMRELAATGFMSNRGRQNVASFLTKNLGIHWHMGAEWFESRLIDYDVASNWGNWNYTAGV
GNDARGFRFFNILKQARDYDPDGAYVKHWLPELAGLPPARVHEPWKLLPVEQKRFGVRLGVDYPQPVVDLFQSAAANEAI
YNAAWEHHHRKRRGQPRSRT
>Mature_499_residues
STKTVLVWYRNDLRVHDHEPLTSALHKNARVVALYCFDPRQFGKAPFGFEKTGPFRARFLLESVADLRRSLRQLGSDLLV
RRGHPEEVIPALVSELEIAAVHYHGEVTSEELVVERDLQAALAPLNVPVRSFWGTTLVHPDDLPFAIEAIPELFTDFRKQ
VERSAAINPPLPAPAKLPPLPAVDPGEIPQLADLGLESPVTDERAVLQFKGGETSGLARLEEYFWQKSLLKSYKQTRNGM
LGADYSSKFSAWLALGCLSARYIHEQVQTYETKRIKNDSTYWLIFELLWRDYFRFIAAKHGDRLFYTAGLRGLDIPWKED
WERFELWRTGQTGFPLVDANMRELAATGFMSNRGRQNVASFLTKNLGIHWHMGAEWFESRLIDYDVASNWGNWNYTAGVG
NDARGFRFFNILKQARDYDPDGAYVKHWLPELAGLPPARVHEPWKLLPVEQKRFGVRLGVDYPQPVVDLFQSAAANEAIY
NAAWEHHHRKRRGQPRSRT

Specific function: May have a photoreceptor function. Binds DNA; probably functions as a transcriptional repressor

COG id: COG0415

COG function: function code L; Deoxyribodipyrimidine photolyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DNA photolyase domain

Homologues:

Organism=Homo sapiens, GI4758072, Length=503, Percent_Identity=29.2246520874751, Blast_Score=182, Evalue=6e-46,
Organism=Homo sapiens, GI188536100, Length=502, Percent_Identity=29.2828685258964, Blast_Score=182, Evalue=7e-46,
Organism=Homo sapiens, GI188536103, Length=453, Percent_Identity=29.5805739514349, Blast_Score=162, Evalue=9e-40,
Organism=Escherichia coli, GI1786926, Length=488, Percent_Identity=29.9180327868852, Blast_Score=196, Evalue=2e-51,
Organism=Saccharomyces cerevisiae, GI6324962, Length=527, Percent_Identity=26.7552182163188, Blast_Score=149, Evalue=9e-37,
Organism=Drosophila melanogaster, GI17137248, Length=508, Percent_Identity=27.3622047244095, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24585455, Length=508, Percent_Identity=27.3622047244095, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24648152, Length=517, Percent_Identity=27.4661508704062, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CRYD_GLOVI (Q7NMD1)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923781.1
- ProteinModelPortal:   Q7NMD1
- SMR:   Q7NMD1
- GeneID:   2599355
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.835
- HOGENOM:   HBG693486
- OMA:   GAEWFES
- ProtClustDB:   CLSK892974
- BioCyc:   GVIO251221:GLR0835-MONOMER
- GO:   GO:0006350
- InterPro:   IPR002081
- InterPro:   IPR014133
- InterPro:   IPR018394
- InterPro:   IPR006050
- InterPro:   IPR005101
- InterPro:   IPR014729
- Gene3D:   G3DSA:3.40.50.620
- PRINTS:   PR00147
- TIGRFAMs:   TIGR02765

Pfam domain/function: PF00875 DNA_photolyase; PF03441 FAD_binding_7; SSF52425 DNA_photolyase_N; SSF48173 Photolyase_FAD-bd/Cryptochr_C

EC number: 4.1.99.3 [C]

Molecular weight: Translated: 57264; Mature: 57132

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS00394 DNA_PHOTOLYASES_1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKTVLVWYRNDLRVHDHEPLTSALHKNARVVALYCFDPRQFGKAPFGFEKTGPFRARF
CCCCEEEEEECCCCEECCCCHHHHHHCCCCEEEEEEEECHHHHCCCCCCCCCCCCHHHHH
LLESVADLRRSLRQLGSDLLVRRGHPEEVIPALVSELEIAAVHYHGEVTSEELVVERDLQ
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHH
AALAPLNVPVRSFWGTTLVHPDDLPFAIEAIPELFTDFRKQVERSAAINPPLPAPAKLPP
HHHCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
LPAVDPGEIPQLADLGLESPVTDERAVLQFKGGETSGLARLEEYFWQKSLLKSYKQTRNG
CCCCCCCCCCHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
MLGADYSSKFSAWLALGCLSARYIHEQVQTYETKRIKNDSTYWLIFELLWRDYFRFIAAK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
HGDRLFYTAGLRGLDIPWKEDWERFELWRTGQTGFPLVDANMRELAATGFMSNRGRQNVA
CCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHCCCHHHHH
SFLTKNLGIHWHMGAEWFESRLIDYDVASNWGNWNYTAGVGNDARGFRFFNILKQARDYD
HHHHHHCCEEEECCHHHHHHHCCCCHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCC
PDGAYVKHWLPELAGLPPARVHEPWKLLPVEQKRFGVRLGVDYPQPVVDLFQSAAANEAI
CCCHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHCCEEECCCCCHHHHHHHHHHHHHHHH
YNAAWEHHHRKRRGQPRSRT
HHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
STKTVLVWYRNDLRVHDHEPLTSALHKNARVVALYCFDPRQFGKAPFGFEKTGPFRARF
CCCEEEEEECCCCEECCCCHHHHHHCCCCEEEEEEEECHHHHCCCCCCCCCCCCHHHHH
LLESVADLRRSLRQLGSDLLVRRGHPEEVIPALVSELEIAAVHYHGEVTSEELVVERDLQ
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHEEEEEECCCCCHHHHHHHHHHH
AALAPLNVPVRSFWGTTLVHPDDLPFAIEAIPELFTDFRKQVERSAAINPPLPAPAKLPP
HHHCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
LPAVDPGEIPQLADLGLESPVTDERAVLQFKGGETSGLARLEEYFWQKSLLKSYKQTRNG
CCCCCCCCCCHHHHCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
MLGADYSSKFSAWLALGCLSARYIHEQVQTYETKRIKNDSTYWLIFELLWRDYFRFIAAK
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
HGDRLFYTAGLRGLDIPWKEDWERFELWRTGQTGFPLVDANMRELAATGFMSNRGRQNVA
CCCEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHHHCCCHHHHH
SFLTKNLGIHWHMGAEWFESRLIDYDVASNWGNWNYTAGVGNDARGFRFFNILKQARDYD
HHHHHHCCEEEECCHHHHHHHCCCCHHHCCCCCCEEECCCCCCCCHHHHHHHHHHHCCCC
PDGAYVKHWLPELAGLPPARVHEPWKLLPVEQKRFGVRLGVDYPQPVVDLFQSAAANEAI
CCCHHHHHHHHHHHCCCCHHCCCCCCCCCCHHHHCCEEECCCCCHHHHHHHHHHHHHHHH
YNAAWEHHHRKRRGQPRSRT
HHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: ATP; FADH2; Flavin; pterin [C]

Metal ions: NA

Kcat value (1/min): 2.4 [C]

Specific activity: NA

Km value (mM): NA

Substrates: cyclobutadipyrimidine (in DNA) [C]

Specific reaction: cyclobutadipyrimidine (in DNA) = 2 pyrimidine residues (in DNA) [C]

General reaction: C-C-bond cleavage [C]

Inhibitor: yeast DNA [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292