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The map label for this gene is tgt

Identifier: 37520371

GI number: 37520371

Start: 853944

End: 855080

Strand: Direct

Name: tgt

Synonym: glr0802

Alternate gene names: 37520371

Gene position: 853944-855080 (Clockwise)

Preceding gene: 37520370

Following gene: 37520372

Centisome position: 18.33

GC content: 65.96

Gene sequence:

>1137_bases
TTGACGGCTAGTTTTGCCTTCACAATCGAGCACCGCGACGGCGAAGCCCGCGCCGGTACCTTCGCCACCCCCCATGGACC
GGTGTACACGCCCTGCTTCATGCCGGTGGGCACCCAGGCGACCGTCAAAACCCTGACACCCGCCCAACTCGCCGAGACTG
GCGCCCAGATGATTTTGGCCAACACCTATCACCTGAGTCTGCAGCCGGGCGCGGATATCGTCGCGGGAGCCGGTGGACTG
CACGGGTTTATGCAGTGGCCGGGGCCGATTCTCACCGACTCCGGGGGCTTCCAGGTCTTTAGCTTGAGCAGCCTGCGCAC
GATCGACGACGACGGGGTGACTTTTCGCGAACCGAAAAGCGGCGCCCTCGTGCGCTTTACACCCGAGCACGCCGTCGCTG
TGCAAAACGCGCTCGGGGCGGATGTAATCATGGCCTTCGACGAGTGCCCACCTTACCCGGCCGACCGCGAGCAGGTCGAA
GGGGCGGTGGAGCGCACTTTGCGCTGGTTCGAGCGCTGTGTGGAGGCCCACCGCCGCAGCGATCAGGCGTTGTTCGGCAT
CGTGCAGGGCGGCGTCTGGCCGGATCTGCGCCGCCGCTGCGCCGAGGGGCTCGTCGCGGCGGATCTGCCCGGCTACGCCA
TCGGCGGGGTGAGCGTCGGTGAGCCGCAAACGCTCATCGAGCGGGTGGTGCGCGTCACGGCCCCTCTGTTGCCGGAGCAC
AAGCCGCGCTACTTGATGGGAGTAGGCACCTTCCGCGAAATGGCCCAGGCGGTGGCCGTGGGGGTGGATCTGTTCGACTG
CGTGATGCCCACCCGCGTCGCCCGCCACGGCAGTGCCCTATTGCTCGGCACCGGGGGCGACCGTCGCATCAACCTCAAAA
ACGCCCAATTTCGCCGCGACTACGAACCGCTCGACTGCGTGTGCCCTTGCTATACCTGCCGTCACTTCAGCCGCGCCTAC
CTTGCCCACCTGGTGCGCAGCGAAGAGATCCTGGCGATGACGCTGCTGTCGATTCACAACGTCGCCACACTCACCCGCTT
CGCCGCCTTGCTGCGCTGCGCAATCGCTACCGGGAGCTTCGCGCAGGAGTTTGCGCACTATTTGCAAAGCGGGCCTGAGC
CCGTCCTCAGCAATTAA

Upstream 100 bases:

>100_bases
CAGCAGCGGCTCAACACGGGTCTGCGCTCCGTGGTCGAAGAGGTGCTCATGGAGTCTTCGTGCCTTTGCGAGGATTGGGA
CGAGGACGACGACGACGACC

Downstream 100 bases:

>100_bases
ATCAAACTCATAATGTCCATAAGCAACGATTATCTAAAAAAGAAAGATCTTCAGAGCTTAAAAACTCAGTAAAATAGAGC
GGGATAAGTGCAACCCTGCC

Product: transfer RNA-guanine transglycosylase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MTASFAFTIEHRDGEARAGTFATPHGPVYTPCFMPVGTQATVKTLTPAQLAETGAQMILANTYHLSLQPGADIVAGAGGL
HGFMQWPGPILTDSGGFQVFSLSSLRTIDDDGVTFREPKSGALVRFTPEHAVAVQNALGADVIMAFDECPPYPADREQVE
GAVERTLRWFERCVEAHRRSDQALFGIVQGGVWPDLRRRCAEGLVAADLPGYAIGGVSVGEPQTLIERVVRVTAPLLPEH
KPRYLMGVGTFREMAQAVAVGVDLFDCVMPTRVARHGSALLLGTGGDRRINLKNAQFRRDYEPLDCVCPCYTCRHFSRAY
LAHLVRSEEILAMTLLSIHNVATLTRFAALLRCAIATGSFAQEFAHYLQSGPEPVLSN

Sequences:

>Translated_378_residues
MTASFAFTIEHRDGEARAGTFATPHGPVYTPCFMPVGTQATVKTLTPAQLAETGAQMILANTYHLSLQPGADIVAGAGGL
HGFMQWPGPILTDSGGFQVFSLSSLRTIDDDGVTFREPKSGALVRFTPEHAVAVQNALGADVIMAFDECPPYPADREQVE
GAVERTLRWFERCVEAHRRSDQALFGIVQGGVWPDLRRRCAEGLVAADLPGYAIGGVSVGEPQTLIERVVRVTAPLLPEH
KPRYLMGVGTFREMAQAVAVGVDLFDCVMPTRVARHGSALLLGTGGDRRINLKNAQFRRDYEPLDCVCPCYTCRHFSRAY
LAHLVRSEEILAMTLLSIHNVATLTRFAALLRCAIATGSFAQEFAHYLQSGPEPVLSN
>Mature_377_residues
TASFAFTIEHRDGEARAGTFATPHGPVYTPCFMPVGTQATVKTLTPAQLAETGAQMILANTYHLSLQPGADIVAGAGGLH
GFMQWPGPILTDSGGFQVFSLSSLRTIDDDGVTFREPKSGALVRFTPEHAVAVQNALGADVIMAFDECPPYPADREQVEG
AVERTLRWFERCVEAHRRSDQALFGIVQGGVWPDLRRRCAEGLVAADLPGYAIGGVSVGEPQTLIERVVRVTAPLLPEHK
PRYLMGVGTFREMAQAVAVGVDLFDCVMPTRVARHGSALLLGTGGDRRINLKNAQFRRDYEPLDCVCPCYTCRHFSRAYL
AHLVRSEEILAMTLLSIHNVATLTRFAALLRCAIATGSFAQEFAHYLQSGPEPVLSN

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family

Homologues:

Organism=Homo sapiens, GI13654276, Length=346, Percent_Identity=46.5317919075145, Blast_Score=318, Evalue=4e-87,
Organism=Escherichia coli, GI1786607, Length=374, Percent_Identity=47.5935828877005, Blast_Score=349, Evalue=2e-97,
Organism=Caenorhabditis elegans, GI17544680, Length=371, Percent_Identity=40.9703504043127, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI25146502, Length=260, Percent_Identity=24.6153846153846, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI19920510, Length=356, Percent_Identity=46.9101123595506, Blast_Score=329, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TGT_GLOVI (Q7NMG4)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923748.1
- ProteinModelPortal:   Q7NMG4
- SMR:   Q7NMG4
- GeneID:   2599322
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:glr0802
- NMPDR:   fig|251221.1.peg.802
- HOGENOM:   HBG629929
- OMA:   RCIAAHK
- BioCyc:   GVIO251221:GLR0802-MONOMER
- BRENDA:   2.4.2.29
- HAMAP:   MF_00168
- InterPro:   IPR004803
- InterPro:   IPR002616
- Gene3D:   G3DSA:3.20.20.105
- PANTHER:   PTHR11962
- TIGRFAMs:   TIGR00430
- TIGRFAMs:   TIGR00449

Pfam domain/function: PF01702 TGT; SSF51713 tRNA_ribo_trans

EC number: =2.4.2.29

Molecular weight: Translated: 41054; Mature: 40923

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: ACT_SITE 93-93 BINDING 94-94

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASFAFTIEHRDGEARAGTFATPHGPVYTPCFMPVGTQATVKTLTPAQLAETGAQMILA
CCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHCCCCEEEE
NTYHLSLQPGADIVAGAGGLHGFMQWPGPILTDSGGFQVFSLSSLRTIDDDGVTFREPKS
EEEEEEECCCCCEEECCCCCCHHHHCCCCEEECCCCEEEEEECCCCEECCCCCEEECCCC
GALVRFTPEHAVAVQNALGADVIMAFDECPPYPADREQVEGAVERTLRWFERCVEAHRRS
CCEEEECCHHHHHHHHHCCHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DQALFGIVQGGVWPDLRRRCAEGLVAADLPGYAIGGVSVGEPQTLIERVVRVTAPLLPEH
CHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCC
KPRYLMGVGTFREMAQAVAVGVDLFDCVMPTRVARHGSALLLGTGGDRRINLKNAQFRRD
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCHHHCC
YEPLDCVCPCYTCRHFSRAYLAHLVRSEEILAMTLLSIHNVATLTRFAALLRCAIATGSF
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AQEFAHYLQSGPEPVLSN
HHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TASFAFTIEHRDGEARAGTFATPHGPVYTPCFMPVGTQATVKTLTPAQLAETGAQMILA
CCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCHHHHHHCCCCEEEE
NTYHLSLQPGADIVAGAGGLHGFMQWPGPILTDSGGFQVFSLSSLRTIDDDGVTFREPKS
EEEEEEECCCCCEEECCCCCCHHHHCCCCEEECCCCEEEEEECCCCEECCCCCEEECCCC
GALVRFTPEHAVAVQNALGADVIMAFDECPPYPADREQVEGAVERTLRWFERCVEAHRRS
CCEEEECCHHHHHHHHHCCHHHEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
DQALFGIVQGGVWPDLRRRCAEGLVAADLPGYAIGGVSVGEPQTLIERVVRVTAPLLPEH
CHHHHHHHCCCCCHHHHHHHHCCCEEECCCCCEECCCCCCCHHHHHHHHHHHHHCCCCCC
KPRYLMGVGTFREMAQAVAVGVDLFDCVMPTRVARHGSALLLGTGGDRRINLKNAQFRRD
CCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEEECCCHHHCC
YEPLDCVCPCYTCRHFSRAYLAHLVRSEEILAMTLLSIHNVATLTRFAALLRCAIATGSF
CCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
AQEFAHYLQSGPEPVLSN
HHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14621292