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The map label for this gene is panB [H]

Identifier: 37520331

GI number: 37520331

Start: 815384

End: 816172

Strand: Reverse

Name: panB [H]

Synonym: gvip087

Alternate gene names: 37520331

Gene position: 816172-815384 (Counterclockwise)

Preceding gene: 37520332

Following gene: 37520329

Centisome position: 17.52

GC content: 63.88

Gene sequence:

>789_bases
GTGGGCATGGGGTTATCCATCCACAGTCTCAGAAAGCTCAAAGAGCGCGCCCAACCGATTGTCGCCCTTACCGCCACCGA
GTACGCGATGGCCCGCATTCTCGATCAAGCGGGGGTCGATCTGCTGCTGGTGGGCGACTCGCTTTCGATGGTCGCCCTGG
GCCATACGAGCACCCTGCCGGTGACAGTGGACGAGTTGTTGCACCACTGCCGGGCAGTGCGCCGGGGGGTCGAGCGCGCT
TTGCTGGTAGCCGATTTGCCCTTCGGTTCCTACGAGCAGAGCCCCCAGCAAGCTTTTTCCACCGCGACCCGATTTCTCAA
AGAAGCGGGAGTGCAGGCGGTCAAGCTCGAAGGCGGTTACCCGCGCATGACCGAGACGGTCGCTTTTTTGACCGAAAGCG
GCATCCCGGTGCTCGGGCATCTCGGCCTCACCCCCCAGGCGGTTCACCAGTTGGGGGGCTACCGCGTCCAGGGCAAGACC
GCCCAGGCGGCCGAACGGCTCATCGATCAGGCTCTCGCCCTGGAGGGGGCGGGGGCGTTCGCGCTCGTGCTGGAGCACAT
TCCCTACGATCTGGCGGGGGAAATTAGCGGCAAACTGGTCATCCCGACTGTCGGCATCGGCGCCGGTCCGCACTGCAACG
GCCAGATCCTCGTCACCCACGATCTATTGGGCCTTACTGAGAATCCGCCGCCTTTTGTAAATCCTTACGCCGACTTGCGC
GCGCTTATCAGCAAAGCGGTCGGCCGCTACAGTGCGGATGTGCGCGCCCATGGCTCCCGTGAGGAGTGA

Upstream 100 bases:

>100_bases
TTCGACTGGCCCGAAGTCGAAGATATCGGTCTGTACGCCGCGCCGGGTTTCAAGCTGCTGCGCCCGGCCCGATAATCGCG
CCACCCGGCTGTGGCACACT

Downstream 100 bases:

>100_bases
TGCTCACTTCAAGCGTCAGTCGGAGCTACATGTAAATTTTGTCAAATGTCTGGGATCAAGAAAATATAGCTGTATTGCGC
AGCAATGTCCCAAACCGCGG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT [H]

Number of amino acids: Translated: 262; Mature: 261

Protein sequence:

>262_residues
MGMGLSIHSLRKLKERAQPIVALTATEYAMARILDQAGVDLLLVGDSLSMVALGHTSTLPVTVDELLHHCRAVRRGVERA
LLVADLPFGSYEQSPQQAFSTATRFLKEAGVQAVKLEGGYPRMTETVAFLTESGIPVLGHLGLTPQAVHQLGGYRVQGKT
AQAAERLIDQALALEGAGAFALVLEHIPYDLAGEISGKLVIPTVGIGAGPHCNGQILVTHDLLGLTENPPPFVNPYADLR
ALISKAVGRYSADVRAHGSREE

Sequences:

>Translated_262_residues
MGMGLSIHSLRKLKERAQPIVALTATEYAMARILDQAGVDLLLVGDSLSMVALGHTSTLPVTVDELLHHCRAVRRGVERA
LLVADLPFGSYEQSPQQAFSTATRFLKEAGVQAVKLEGGYPRMTETVAFLTESGIPVLGHLGLTPQAVHQLGGYRVQGKT
AQAAERLIDQALALEGAGAFALVLEHIPYDLAGEISGKLVIPTVGIGAGPHCNGQILVTHDLLGLTENPPPFVNPYADLR
ALISKAVGRYSADVRAHGSREE
>Mature_261_residues
GMGLSIHSLRKLKERAQPIVALTATEYAMARILDQAGVDLLLVGDSLSMVALGHTSTLPVTVDELLHHCRAVRRGVERAL
LVADLPFGSYEQSPQQAFSTATRFLKEAGVQAVKLEGGYPRMTETVAFLTESGIPVLGHLGLTPQAVHQLGGYRVQGKTA
QAAERLIDQALALEGAGAFALVLEHIPYDLAGEISGKLVIPTVGIGAGPHCNGQILVTHDLLGLTENPPPFVNPYADLRA
LISKAVGRYSADVRAHGSREE

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate [H]

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family [H]

Homologues:

Organism=Escherichia coli, GI1786326, Length=252, Percent_Identity=46.4285714285714, Blast_Score=210, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6319653, Length=257, Percent_Identity=35.7976653696498, Blast_Score=175, Evalue=5e-45,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003700
- InterPro:   IPR015813 [H]

Pfam domain/function: PF02548 Pantoate_transf [H]

EC number: =2.1.2.11 [H]

Molecular weight: Translated: 27866; Mature: 27734

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGMGLSIHSLRKLKERAQPIVALTATEYAMARILDQAGVDLLLVGDSLSMVALGHTSTLP
CCCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCC
VTVDELLHHCRAVRRGVERALLVADLPFGSYEQSPQQAFSTATRFLKEAGVQAVKLEGGY
EEHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
PRMTETVAFLTESGIPVLGHLGLTPQAVHQLGGYRVQGKTAQAAERLIDQALALEGAGAF
CHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCHH
ALVLEHIPYDLAGEISGKLVIPTVGIGAGPHCNGQILVTHDLLGLTENPPPFVNPYADLR
HHHHHHCCCCHHHCCCCEEEEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHH
ALISKAVGRYSADVRAHGSREE
HHHHHHHHHHCCCHHCCCCCCC
>Mature Secondary Structure 
GMGLSIHSLRKLKERAQPIVALTATEYAMARILDQAGVDLLLVGDSLSMVALGHTSTLP
CCCCCHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHCCCEEEEECCCCEEEEECCCCCCC
VTVDELLHHCRAVRRGVERALLVADLPFGSYEQSPQQAFSTATRFLKEAGVQAVKLEGGY
EEHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCC
PRMTETVAFLTESGIPVLGHLGLTPQAVHQLGGYRVQGKTAQAAERLIDQALALEGAGAF
CHHHHHHHHHHCCCCCEEEECCCCHHHHHHHCCEEEECCHHHHHHHHHHHHHHHCCCCHH
ALVLEHIPYDLAGEISGKLVIPTVGIGAGPHCNGQILVTHDLLGLTENPPPFVNPYADLR
HHHHHHCCCCHHHCCCCEEEEEEECCCCCCCCCCEEEEEEEEECCCCCCCCCCCCHHHHH
ALISKAVGRYSADVRAHGSREE
HHHHHHHHHHCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]