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The map label for this gene is hisA

Identifier: 37520275

GI number: 37520275

Start: 751221

End: 751988

Strand: Direct

Name: hisA

Synonym: gvip072

Alternate gene names: 37520275

Gene position: 751221-751988 (Clockwise)

Preceding gene: 37520273

Following gene: 37520276

Centisome position: 16.12

GC content: 68.23

Gene sequence:

>768_bases
ATGGATATTATTCCTGCGATTGACATACTCGACGGCCGCTGCGTGCGCCTCTACCAGGGCAACTACCAGCTTGCTGAGAC
CTACGGGGAGGACCCGGTGGCCGTTGCCTGCAACTGGGCGAAACTGGGGGCGCCGCGGCTGCACGTGGTCGACCTGGACG
GTGCGCGCCAGGGGATGCCCGTGCACCTCGACGCCCTCGAAGCGATCGTCACCCAGGTGCCCTGCCCGGTACAGTTCGGG
GGTGGCCTGCGCAGCATCGAGGCGGTCAGTGCCGTGCTCGATCGCGGTGTGGACCGGGTGATCCTGGGCACCGCCGCGGT
CGAAAATCCGGCGCTTATCCGCGAGTGCTGCGAGCGCTTCGGCGGCAGAATCGCCGTCGGCCTCGACGCGCGCGGCGGGC
AGGTGGCGGTGCGCGGCTGGCGGGAAACCTCGGAAGTGGAGGTGACCGAACTGGCCGGCGAGATGGAAAAGCTCGGCGTC
AGCGCCATCGTCTACACCGACATCCTCAAAGACGGTACCCTCACCGGTCCGAACCTGGTGGAGCTGCAGCGGCTCACCGA
CGCGGTCAAAGTGCCGATCATCGCCTCCGGCGGCGTGGGCACCCTGGCGGATGTGCTCTACCTGCTGGCGCTGGAGCCGC
GCGGATTGCAAGGAGTGATTATCGGCCGGGCCCTCTACACGGGCGATGTCGATCTGGCCGAGGCGCTCAGGGCGGCCGGG
CCGAGCCGCTGGCAGGATCTTCCCCCGGACGATGTGGCCTTCGCCTGA

Upstream 100 bases:

>100_bases
CCATAGAAAAAATCCAGCCCAGCCGGTTGCGCATCGCTCCCATTTAACATTAGTTTTCGGGGATTGCTGCGGGCCGGGGC
AACGGCCAATAATGTGAGCC

Downstream 100 bases:

>100_bases
TGGTGGGTGATACCCTAGAGCAGCTTTTGCCGCATTGGATGCTCTCTATGGTCCGGTTGCTCTTTTGTTCGATGCTTGCT
TTGTCGCTTGCGGGTCCTTT

Product: phosphorybosilformimino-5-amino-phosphorybosil- 4-imidazolecarboxamideisomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MDIIPAIDILDGRCVRLYQGNYQLAETYGEDPVAVACNWAKLGAPRLHVVDLDGARQGMPVHLDALEAIVTQVPCPVQFG
GGLRSIEAVSAVLDRGVDRVILGTAAVENPALIRECCERFGGRIAVGLDARGGQVAVRGWRETSEVEVTELAGEMEKLGV
SAIVYTDILKDGTLTGPNLVELQRLTDAVKVPIIASGGVGTLADVLYLLALEPRGLQGVIIGRALYTGDVDLAEALRAAG
PSRWQDLPPDDVAFA

Sequences:

>Translated_255_residues
MDIIPAIDILDGRCVRLYQGNYQLAETYGEDPVAVACNWAKLGAPRLHVVDLDGARQGMPVHLDALEAIVTQVPCPVQFG
GGLRSIEAVSAVLDRGVDRVILGTAAVENPALIRECCERFGGRIAVGLDARGGQVAVRGWRETSEVEVTELAGEMEKLGV
SAIVYTDILKDGTLTGPNLVELQRLTDAVKVPIIASGGVGTLADVLYLLALEPRGLQGVIIGRALYTGDVDLAEALRAAG
PSRWQDLPPDDVAFA
>Mature_255_residues
MDIIPAIDILDGRCVRLYQGNYQLAETYGEDPVAVACNWAKLGAPRLHVVDLDGARQGMPVHLDALEAIVTQVPCPVQFG
GGLRSIEAVSAVLDRGVDRVILGTAAVENPALIRECCERFGGRIAVGLDARGGQVAVRGWRETSEVEVTELAGEMEKLGV
SAIVYTDILKDGTLTGPNLVELQRLTDAVKVPIIASGGVGTLADVLYLLALEPRGLQGVIIGRALYTGDVDLAEALRAAG
PSRWQDLPPDDVAFA

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family

Homologues:

Organism=Escherichia coli, GI87082028, Length=251, Percent_Identity=38.6454183266932, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1788336, Length=243, Percent_Identity=27.1604938271605, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS4_GLOVI (Q7NMQ9)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923652.1
- ProteinModelPortal:   Q7NMQ9
- SMR:   Q7NMQ9
- GeneID:   2599425
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.706
- HOGENOM:   HBG541613
- OMA:   SIIYTDI
- BioCyc:   GVIO251221:GLR0706-MONOMER
- BRENDA:   5.3.1.16
- GO:   GO:0005737
- HAMAP:   MF_01014
- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00007

Pfam domain/function: PF00977 His_biosynth; SSF51366 RibP_bind_barrel

EC number: =5.3.1.16

Molecular weight: Translated: 27018; Mature: 27018

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: ACT_SITE 8-8 ACT_SITE 129-129

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIIPAIDILDGRCVRLYQGNYQLAETYGEDPVAVACNWAKLGAPRLHVVDLDGARQGMP
CCCCCCEECCCCCEEEEEECCEEEEHHCCCCCEEEEECHHHCCCCEEEEEECCCCCCCCC
VHLDALEAIVTQVPCPVQFGGGLRSIEAVSAVLDRGVDRVILGTAAVENPALIRECCERF
EEHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCEEEEEHHCCCCCHHHHHHHHHH
GGRIAVGLDARGGQVAVRGWRETSEVEVTELAGEMEKLGVSAIVYTDILKDGTLTGPNLV
CCEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHH
ELQRLTDAVKVPIIASGGVGTLADVLYLLALEPRGLQGVIIGRALYTGDVDLAEALRAAG
HHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHHHHHHCC
PSRWQDLPPDDVAFA
CCCCCCCCCCCCCCC
>Mature Secondary Structure
MDIIPAIDILDGRCVRLYQGNYQLAETYGEDPVAVACNWAKLGAPRLHVVDLDGARQGMP
CCCCCCEECCCCCEEEEEECCEEEEHHCCCCCEEEEECHHHCCCCEEEEEECCCCCCCCC
VHLDALEAIVTQVPCPVQFGGGLRSIEAVSAVLDRGVDRVILGTAAVENPALIRECCERF
EEHHHHHHHHHHCCCCEECCCCHHHHHHHHHHHHCCCCEEEEEHHCCCCCHHHHHHHHHH
GGRIAVGLDARGGQVAVRGWRETSEVEVTELAGEMEKLGVSAIVYTDILKDGTLTGPNLV
CCEEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHH
ELQRLTDAVKVPIIASGGVGTLADVLYLLALEPRGLQGVIIGRALYTGDVDLAEALRAAG
HHHHHHHHHCCCEEECCCCHHHHHHHHHHHCCCCCCCEEEEEEEEEECCCHHHHHHHHCC
PSRWQDLPPDDVAFA
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292