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The map label for this gene is phoA [H]

Identifier: 37520136

GI number: 37520136

Start: 602712

End: 605072

Strand: Reverse

Name: phoA [H]

Synonym: gll0567

Alternate gene names: 37520136

Gene position: 605072-602712 (Counterclockwise)

Preceding gene: 37520138

Following gene: 37520135

Centisome position: 12.99

GC content: 63.91

Gene sequence:

>2361_bases
ATGAAGCGTTTGAAATGGATACATGTCCTCGGTGCGCTGTCTGCGACAGTCGGTCTGGCATCTTCTTTGGGTGCCGGTCC
CGCCTGGGCCGGGGGCGACGACGACACCCAGCAGAGCAACGTGCCGCCGGTGCTGGGCAACCCGCCTTTTTCCGACAACC
CGACCAACGCCAGCCCAGGCGTGGGCCTGCGGATCCTGCCGCCCACCAATACAACGCTGATTGCCGATCAGCTGTTTGAC
TTGCGCGTCGAGACCCAGGTGCCGGGCACCAGCGGCCGGGCGCCCTTGCTCAAGAGCCTGACCGTCAACGGCACCGAGAT
TGCTCAGCCGTTCAACGACACGATCAAAAAGCAGGGTCTGGGCCTGGAGAGCGGTTCGCCGACGGTGCCGGGTCTTTACG
GCGCGTCTGCGCGCAATTTTGCTTTCCCGCAGGCCGGTCGCTACCGGGTGCGCGCCGTCGTGAGCGTGGACGGTGCCGAC
TACACCATCCAGAACACTTATACCGTCGCCCCCTTCGCCCTCAAGCAAAACATCAACCACGTCGTCTTCTTTTTGGGTGA
CGCGATGGGACTACCGATCCGCTCCGCCGCCCGGATTGCAGGCAAGGGCGTCTTCGAAGGCCGCGCCAAAGGACAGCTCA
ATATGGACACCATGGATACCTACGGCCTGGTGTACACGGCTTCCTTCGACAGCATCATCACCGACTCGGCGCCGGGCATG
GCCAGCTACATCACCGGCATGAAGCAGCCCAATAACGCCTTGAACGTTTCTGTGGACAACACCCCGGAAAATGCCCTGGA
CAATCCGCGCATCGAACCGCTGTGGGCGTACATGAAGCGTAAGTACGGCTGGGCGACCGGCGTGGTGAGCGACGCCTTTG
TCACCGACGCCACCCCGGCTTCTGAGGTGGCCCACTCCCGGGCGCGCTCGGCCCGTACCGCCATCGCCCAGCAGTTTATC
GACTACTACATCGACAACGGTCCTACCGGCACCGCTGCCCAACCGGTCACCGGCTACGCGTCCCTCAAGGCCCTCACCCA
GCCGCTCGATGTGATCATTGGCGGCGGAGCGCGCGACTGGCTGCCAGTCAACGATCCGACGCTGCTCAATTTCTATCAGG
CCAGTTCCGGCCAGGGCCGCCCCGACGGGATCAACCTCTTTACCGTTGCAGCCGGCCAGGGCTACTCAGTGGTCAAGGAC
AAGACCGAATTGGCAAGCGCCCCTAACAACAAACCAATCCTGGGCATCTTCGCAGGAGATTTCCGGCCGGGCAACGCTCT
GGGAGCCGACAACATCCCCGGTACCCTTGACCGGTTGGTCGCCCGCGGCCAGGCGACCATCGGCGGCAAGACCGCGAGCG
ACCCCGAACTGGGGCAGAGCGTGCCGCCGCCGGTGGGCACCGGTTGTGGCGGGACGGTCCAGGCGTGTTTTGCCAATGTT
CCTTCCAAGACCGAGATGGTGGCCAAGGCCATCGAGGTGCTCAACGCCAAAAACCCGAACGGCTGGATGCTGATGGTCGA
GCAGTCGCAGACCGACAAACTGGCGCACCCGCTGGAGTACGAGCGGGTGATCTACGAGGCGCTGGAGCTGGATAACGCCC
TGGGCTATGTGCTCAACGGCCAGGCCACGGACGGCAAAACCCTTGCCCTCATCACCGCTGACCATGCCCAGCCTGAAACG
ATCGTCGGCATCACCGTGCCGAGCGCCCTCGACAACCTGCCAGGCTACCTGGGCGACCCCTCGGGCACCGACCCGATCAC
CCCGGGCGGCTGCTTCACCAACTCGATCAGCTCCGACGGCACCAGCGGCTCGCTCTCGCTGACGATCGACGGGGCAGGGG
CCGAAACCAAACCCTGTGCGCTGCAGGATGCCATCGGCACCTTCAACGACGGCACCTTCCCAACTTACGTCGATGCCAAC
AAAGACGGCTTCCCGGACGATCCGGATCCGACGGTCAAACTGGTGCTCGACAACGGCGGCAAACCGACCTACAGCCAGGA
CTATCTGACCAACTTCATTCCGCTCAACCCGAGCGGAATTACCGCCGCGGTACCGAACCCGGCGCGCGACCCGAACGGCA
TCCTGCTGACGGGTAACATGTCCACTGCCGACGTTTCGCCCATCAGCAAAGACTCCGGCAACATCGGAGTCGCCCCGCAC
TCCGGCGAGGACGTGCCCCTGAGCGCCAGCGGTCCGAACGCCTTTTTGTTCGGCGGCACCTACGAGAACTCGGACGTGTT
CGTGCGTTTGGCCCGCGCCCTTTCTCTGGAAGGAGCTACGAGTCGTCAGGCGGCGCTGAAGCTGCCCGCCGGGTTCCCGA
GCGTCTCGGCGGAACAACTGTTCCGCGGCAAACAGGAGTAG

Upstream 100 bases:

>100_bases
CTGAAAGCGATTAGTATCTGTTAGTTTTTGCTCCAATCTTCGGAAGCAGCAATCTATTACTGTGCGCATCTGGCTTTGTT
TTTCTTAGGAGTTTTGCGCG

Downstream 100 bases:

>100_bases
AAGCGATGCCACGCCACTGGTTCGTCGTCCTCGGGTTGGGGCTTGCCCTGGCAGCCCCGGCCCAGGCGGAGAGCGTCGTC
TACGGCCGGGTATTCCAGAC

Product: hypothetical protein

Products: NA

Alternate protein names: High molecular weight phosphatase; H-AP [H]

Number of amino acids: Translated: 786; Mature: 786

Protein sequence:

>786_residues
MKRLKWIHVLGALSATVGLASSLGAGPAWAGGDDDTQQSNVPPVLGNPPFSDNPTNASPGVGLRILPPTNTTLIADQLFD
LRVETQVPGTSGRAPLLKSLTVNGTEIAQPFNDTIKKQGLGLESGSPTVPGLYGASARNFAFPQAGRYRVRAVVSVDGAD
YTIQNTYTVAPFALKQNINHVVFFLGDAMGLPIRSAARIAGKGVFEGRAKGQLNMDTMDTYGLVYTASFDSIITDSAPGM
ASYITGMKQPNNALNVSVDNTPENALDNPRIEPLWAYMKRKYGWATGVVSDAFVTDATPASEVAHSRARSARTAIAQQFI
DYYIDNGPTGTAAQPVTGYASLKALTQPLDVIIGGGARDWLPVNDPTLLNFYQASSGQGRPDGINLFTVAAGQGYSVVKD
KTELASAPNNKPILGIFAGDFRPGNALGADNIPGTLDRLVARGQATIGGKTASDPELGQSVPPPVGTGCGGTVQACFANV
PSKTEMVAKAIEVLNAKNPNGWMLMVEQSQTDKLAHPLEYERVIYEALELDNALGYVLNGQATDGKTLALITADHAQPET
IVGITVPSALDNLPGYLGDPSGTDPITPGGCFTNSISSDGTSGSLSLTIDGAGAETKPCALQDAIGTFNDGTFPTYVDAN
KDGFPDDPDPTVKLVLDNGGKPTYSQDYLTNFIPLNPSGITAAVPNPARDPNGILLTGNMSTADVSPISKDSGNIGVAPH
SGEDVPLSASGPNAFLFGGTYENSDVFVRLARALSLEGATSRQAALKLPAGFPSVSAEQLFRGKQE

Sequences:

>Translated_786_residues
MKRLKWIHVLGALSATVGLASSLGAGPAWAGGDDDTQQSNVPPVLGNPPFSDNPTNASPGVGLRILPPTNTTLIADQLFD
LRVETQVPGTSGRAPLLKSLTVNGTEIAQPFNDTIKKQGLGLESGSPTVPGLYGASARNFAFPQAGRYRVRAVVSVDGAD
YTIQNTYTVAPFALKQNINHVVFFLGDAMGLPIRSAARIAGKGVFEGRAKGQLNMDTMDTYGLVYTASFDSIITDSAPGM
ASYITGMKQPNNALNVSVDNTPENALDNPRIEPLWAYMKRKYGWATGVVSDAFVTDATPASEVAHSRARSARTAIAQQFI
DYYIDNGPTGTAAQPVTGYASLKALTQPLDVIIGGGARDWLPVNDPTLLNFYQASSGQGRPDGINLFTVAAGQGYSVVKD
KTELASAPNNKPILGIFAGDFRPGNALGADNIPGTLDRLVARGQATIGGKTASDPELGQSVPPPVGTGCGGTVQACFANV
PSKTEMVAKAIEVLNAKNPNGWMLMVEQSQTDKLAHPLEYERVIYEALELDNALGYVLNGQATDGKTLALITADHAQPET
IVGITVPSALDNLPGYLGDPSGTDPITPGGCFTNSISSDGTSGSLSLTIDGAGAETKPCALQDAIGTFNDGTFPTYVDAN
KDGFPDDPDPTVKLVLDNGGKPTYSQDYLTNFIPLNPSGITAAVPNPARDPNGILLTGNMSTADVSPISKDSGNIGVAPH
SGEDVPLSASGPNAFLFGGTYENSDVFVRLARALSLEGATSRQAALKLPAGFPSVSAEQLFRGKQE
>Mature_786_residues
MKRLKWIHVLGALSATVGLASSLGAGPAWAGGDDDTQQSNVPPVLGNPPFSDNPTNASPGVGLRILPPTNTTLIADQLFD
LRVETQVPGTSGRAPLLKSLTVNGTEIAQPFNDTIKKQGLGLESGSPTVPGLYGASARNFAFPQAGRYRVRAVVSVDGAD
YTIQNTYTVAPFALKQNINHVVFFLGDAMGLPIRSAARIAGKGVFEGRAKGQLNMDTMDTYGLVYTASFDSIITDSAPGM
ASYITGMKQPNNALNVSVDNTPENALDNPRIEPLWAYMKRKYGWATGVVSDAFVTDATPASEVAHSRARSARTAIAQQFI
DYYIDNGPTGTAAQPVTGYASLKALTQPLDVIIGGGARDWLPVNDPTLLNFYQASSGQGRPDGINLFTVAAGQGYSVVKD
KTELASAPNNKPILGIFAGDFRPGNALGADNIPGTLDRLVARGQATIGGKTASDPELGQSVPPPVGTGCGGTVQACFANV
PSKTEMVAKAIEVLNAKNPNGWMLMVEQSQTDKLAHPLEYERVIYEALELDNALGYVLNGQATDGKTLALITADHAQPET
IVGITVPSALDNLPGYLGDPSGTDPITPGGCFTNSISSDGTSGSLSLTIDGAGAETKPCALQDAIGTFNDGTFPTYVDAN
KDGFPDDPDPTVKLVLDNGGKPTYSQDYLTNFIPLNPSGITAAVPNPARDPNGILLTGNMSTADVSPISKDSGNIGVAPH
SGEDVPLSASGPNAFLFGGTYENSDVFVRLARALSLEGATSRQAALKLPAGFPSVSAEQLFRGKQE

Specific function: Has only a phosphomonoesterase activity [H]

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Secreted. Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI157266296, Length=393, Percent_Identity=27.2264631043257, Blast_Score=87, Evalue=7e-17,
Organism=Homo sapiens, GI157266292, Length=405, Percent_Identity=25.4320987654321, Blast_Score=84, Evalue=4e-16,
Organism=Escherichia coli, GI48994877, Length=396, Percent_Identity=28.7878787878788, Blast_Score=99, Evalue=9e-22,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 82017; Mature: 82017

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS00092 N6_MTASE ; PS00107 PROTEIN_KINASE_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLKWIHVLGALSATVGLASSLGAGPAWAGGDDDTQQSNVPPVLGNPPFSDNPTNASPG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VGLRILPPTNTTLIADQLFDLRVETQVPGTSGRAPLLKSLTVNGTEIAQPFNDTIKKQGL
CCEEEECCCCCEEEEEHHEEEEEEEECCCCCCCCCHHHEEECCCHHHHCCHHHHHHHCCC
GLESGSPTVPGLYGASARNFAFPQAGRYRVRAVVSVDGADYTIQNTYTVAPFALKQNINH
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEECEEEECCHHHHCCCCE
VVFFLGDAMGLPIRSAARIAGKGVFEGRAKGQLNMDTMDTYGLVYTASFDSIITDSAPGM
EEEEECCCCCCCHHHHHHHHCCCEECCCCCCEECCCCCCCCCEEEEECCCHHHCCCCCCH
ASYITGMKQPNNALNVSVDNTPENALDNPRIEPLWAYMKRKYGWATGVVSDAFVTDATPA
HHHHHCCCCCCCEEEEEECCCCCHHCCCCCCCHHHHHHHHHCCCHHCCCCCCEECCCCCH
SEVAHSRARSARTAIAQQFIDYYIDNGPTGTAAQPVTGYASLKALTQPLDVIIGGGARDW
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCCC
LPVNDPTLLNFYQASSGQGRPDGINLFTVAAGQGYSVVKDKTELASAPNNKPILGIFAGD
CCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCEECCHHHHHCCCCCCCEEEEEECC
FRPGNALGADNIPGTLDRLVARGQATIGGKTASDPELGQSVPPPVGTGCGGTVQACFANV
CCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
PSKTEMVAKAIEVLNAKNPNGWMLMVEQSQTDKLAHPLEYERVIYEALELDNALGYVLNG
CCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHCCCHHHHHHHHHHHHHCCCCCEEEEC
QATDGKTLALITADHAQPETIVGITVPSALDNLPGYLGDPSGTDPITPGGCFTNSISSDG
CCCCCCEEEEEEECCCCCCEEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TSGSLSLTIDGAGAETKPCALQDAIGTFNDGTFPTYVDANKDGFPDDPDPTVKLVLDNGG
CCCEEEEEEECCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCC
KPTYSQDYLTNFIPLNPSGITAAVPNPARDPNGILLTGNMSTADVSPISKDSGNIGVAPH
CCCCCHHHHHHEEECCCCCEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEECCC
SGEDVPLSASGPNAFLFGGTYENSDVFVRLARALSLEGATSRQAALKLPAGFPSVSAEQL
CCCCCEEECCCCCEEEECCEECCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHH
FRGKQE
HCCCCC
>Mature Secondary Structure
MKRLKWIHVLGALSATVGLASSLGAGPAWAGGDDDTQQSNVPPVLGNPPFSDNPTNASPG
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
VGLRILPPTNTTLIADQLFDLRVETQVPGTSGRAPLLKSLTVNGTEIAQPFNDTIKKQGL
CCEEEECCCCCEEEEEHHEEEEEEEECCCCCCCCCHHHEEECCCHHHHCCHHHHHHHCCC
GLESGSPTVPGLYGASARNFAFPQAGRYRVRAVVSVDGADYTIQNTYTVAPFALKQNINH
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEEECCCCEEEECEEEECCHHHHCCCCE
VVFFLGDAMGLPIRSAARIAGKGVFEGRAKGQLNMDTMDTYGLVYTASFDSIITDSAPGM
EEEEECCCCCCCHHHHHHHHCCCEECCCCCCEECCCCCCCCCEEEEECCCHHHCCCCCCH
ASYITGMKQPNNALNVSVDNTPENALDNPRIEPLWAYMKRKYGWATGVVSDAFVTDATPA
HHHHHCCCCCCCEEEEEECCCCCHHCCCCCCCHHHHHHHHHCCCHHCCCCCCEECCCCCH
SEVAHSRARSARTAIAQQFIDYYIDNGPTGTAAQPVTGYASLKALTQPLDVIIGGGARDW
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCEEEEECCCCCCC
LPVNDPTLLNFYQASSGQGRPDGINLFTVAAGQGYSVVKDKTELASAPNNKPILGIFAGD
CCCCCCCEEEEEECCCCCCCCCCEEEEEEECCCCCCEECCHHHHHCCCCCCCEEEEEECC
FRPGNALGADNIPGTLDRLVARGQATIGGKTASDPELGQSVPPPVGTGCGGTVQACFANV
CCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCC
PSKTEMVAKAIEVLNAKNPNGWMLMVEQSQTDKLAHPLEYERVIYEALELDNALGYVLNG
CCHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHCCCHHHHHHHHHHHHHCCCCCEEEEC
QATDGKTLALITADHAQPETIVGITVPSALDNLPGYLGDPSGTDPITPGGCFTNSISSDG
CCCCCCEEEEEEECCCCCCEEEEEECCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TSGSLSLTIDGAGAETKPCALQDAIGTFNDGTFPTYVDANKDGFPDDPDPTVKLVLDNGG
CCCEEEEEEECCCCCCCCCHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEEEEECCC
KPTYSQDYLTNFIPLNPSGITAAVPNPARDPNGILLTGNMSTADVSPISKDSGNIGVAPH
CCCCCHHHHHHEEECCCCCEEEECCCCCCCCCCEEEECCCCCCCCCCCCCCCCCEEECCC
SGEDVPLSASGPNAFLFGGTYENSDVFVRLARALSLEGATSRQAALKLPAGFPSVSAEQL
CCCCCEEECCCCCEEEECCEECCCHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCHHHH
FRGKQE
HCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043; 8454193 [H]