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The map label for this gene is purH [H]

Identifier: 37520128

GI number: 37520128

Start: 594478

End: 595977

Strand: Direct

Name: purH [H]

Synonym: gvip060

Alternate gene names: 37520128

Gene position: 594478-595977 (Clockwise)

Preceding gene: 37520124

Following gene: 37520131

Centisome position: 12.76

GC content: 65.13

Gene sequence:

>1500_bases
ATGCCCATCGCCCTGTTGAGCGTTTCCGACAAGACCGGTCTGGTCGACTTTGCCCGTGCCCTGGTGACGGAATTTGAGTA
TCAGCTCATCAGCTCCGGCGGCACCGCCCGGGCGCTCGCCGAGGCGGGTGTTCCGGTGCAGGAAGTGGGCGATTTTACCG
GTGCCGCCGAGATGCTGGGCGGGCGGGTCAAGACACTGCATCCCAAGATCCACGGCGGCATCCTGGCGCGCCGCGACCAG
GAATCGGACCGGCGCGACCTGGAGACCCACAGCATCGCACCCATCGATCTGGTGGTGGTCAATCTCTACCCGTTCGAGCA
AGATCCGCGCATCGAGCAAATCGACGTGGGCGGCCCGACGATGGTGCGTGCCGCCGCCAAAAACCACGCCTTTGTGACGG
TGCTGGTCTCACCGGGTCAGTACGAGGGGTGCCTGGCCGAGTTGCGCCGCAACCGCGGTGTGACGACGCTGCCCTTTCGC
GCCGCTTGCGCAGCGGCGGCTTTTGCGCGCACCGCCGCCTACGACCGGGCGATCGCCGCGTGGTTTGCCGGTTCTGAACC
GGTCCTGGGCGAGCGGCTAGTCCTGGAACTGGAGCGGGTGCAGCCCTTGCGCTATGGCGAGAATCCTCACCAGGCCGCCG
CCTGGTACCGCCACGGCCCGGCGGCGGGTTGGAGTACGGGTCGCATCCTCCAGGGCAAGGCCCTCAGTTTCAACAACCTC
ATCGACCTGGAGGCAAGTCGCCGCCTGGTGGCCGAATTCGACGACACCCCGGCGGCAGTGATCATCAAGCACACCAATCC
CTGCGGGGTGGCTGCAGACGCCACGATTGCCGCAGCCTACCGCCGTGCCTTCGATGCCGACAGCGCCTCCGCCTTTGGTG
GAATCGTTGCTCTCAACCGGCCCTGCGACGAACCGACAGCCGCCCTGCTGGGCCAGACTTTTCTAGAGTGCGTCGTCGCC
CCCGCCTTCACCCTGGGGGCGTTGGCTTTGCTTGAACGCAAGAAAAACCTGCGCCTGCTGGAACTGGCAGATCTGACCAC
CCCGCCGGTGTTTGACGTCAAGCCCATAAGCGGTGGCTTTTTGATCCAGCAGGTGGACGCGCCGGTGCGCGCTGAGGATT
GGAAAGTGGTCACCGAGGCGGTGCCCGATGAAGACCAAAGAGCGGAGTTACTGTTCGCCTGGAAAGTCTGCCAGCACGTC
AAATCGAACGCTATCGTCGTGAGCCATCTTCGCCAGACGCTGGGGGTCGGCGCCGGTCAGATGAACCGGGTAGGTTCAGC
CCGACTGGCTCTCGCCCAAGCAGGCGAGAAAGCCCGGGGGGCAGTGCTTGCAAGCGACGGCTTTTTTCCGTTCGACGACA
CCGTGCGCGCCGCCGCCGAGCGCGGTATCCGGGCTATCGTTCAGCCCGGGGGCAGCCTGCGCGACGAAGATTCGATCCGC
GCCTGCAACGAACTTGGGGTGGCGATGGTTTTTACCGGTGTGCGCCACTTCTTGCACTGA

Upstream 100 bases:

>100_bases
ACATAGACGCTCCCGGTCACTGGTGTTGCCATTATCGGCGAGGCGATCGGCCCCGGCAACGGCTGTGGCAGAATTGCGGG
AGTCTTTAGATCCATCCGCG

Downstream 100 bases:

>100_bases
GCGCTACGGTTTGCAGGGATAGCGCATCAAAACGGGCTTCACTTCGCTGGTGGTGGAAAAATGGCCCTCGTTGCCGTCGA
TGCCGATCGCCTCGCCCTGG

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MPIALLSVSDKTGLVDFARALVTEFEYQLISSGGTARALAEAGVPVQEVGDFTGAAEMLGGRVKTLHPKIHGGILARRDQ
ESDRRDLETHSIAPIDLVVVNLYPFEQDPRIEQIDVGGPTMVRAAAKNHAFVTVLVSPGQYEGCLAELRRNRGVTTLPFR
AACAAAAFARTAAYDRAIAAWFAGSEPVLGERLVLELERVQPLRYGENPHQAAAWYRHGPAAGWSTGRILQGKALSFNNL
IDLEASRRLVAEFDDTPAAVIIKHTNPCGVAADATIAAAYRRAFDADSASAFGGIVALNRPCDEPTAALLGQTFLECVVA
PAFTLGALALLERKKNLRLLELADLTTPPVFDVKPISGGFLIQQVDAPVRAEDWKVVTEAVPDEDQRAELLFAWKVCQHV
KSNAIVVSHLRQTLGVGAGQMNRVGSARLALAQAGEKARGAVLASDGFFPFDDTVRAAAERGIRAIVQPGGSLRDEDSIR
ACNELGVAMVFTGVRHFLH

Sequences:

>Translated_499_residues
MPIALLSVSDKTGLVDFARALVTEFEYQLISSGGTARALAEAGVPVQEVGDFTGAAEMLGGRVKTLHPKIHGGILARRDQ
ESDRRDLETHSIAPIDLVVVNLYPFEQDPRIEQIDVGGPTMVRAAAKNHAFVTVLVSPGQYEGCLAELRRNRGVTTLPFR
AACAAAAFARTAAYDRAIAAWFAGSEPVLGERLVLELERVQPLRYGENPHQAAAWYRHGPAAGWSTGRILQGKALSFNNL
IDLEASRRLVAEFDDTPAAVIIKHTNPCGVAADATIAAAYRRAFDADSASAFGGIVALNRPCDEPTAALLGQTFLECVVA
PAFTLGALALLERKKNLRLLELADLTTPPVFDVKPISGGFLIQQVDAPVRAEDWKVVTEAVPDEDQRAELLFAWKVCQHV
KSNAIVVSHLRQTLGVGAGQMNRVGSARLALAQAGEKARGAVLASDGFFPFDDTVRAAAERGIRAIVQPGGSLRDEDSIR
ACNELGVAMVFTGVRHFLH
>Mature_498_residues
PIALLSVSDKTGLVDFARALVTEFEYQLISSGGTARALAEAGVPVQEVGDFTGAAEMLGGRVKTLHPKIHGGILARRDQE
SDRRDLETHSIAPIDLVVVNLYPFEQDPRIEQIDVGGPTMVRAAAKNHAFVTVLVSPGQYEGCLAELRRNRGVTTLPFRA
ACAAAAFARTAAYDRAIAAWFAGSEPVLGERLVLELERVQPLRYGENPHQAAAWYRHGPAAGWSTGRILQGKALSFNNLI
DLEASRRLVAEFDDTPAAVIIKHTNPCGVAADATIAAAYRRAFDADSASAFGGIVALNRPCDEPTAALLGQTFLECVVAP
AFTLGALALLERKKNLRLLELADLTTPPVFDVKPISGGFLIQQVDAPVRAEDWKVVTEAVPDEDQRAELLFAWKVCQHVK
SNAIVVSHLRQTLGVGAGQMNRVGSARLALAQAGEKARGAVLASDGFFPFDDTVRAAAERGIRAIVQPGGSLRDEDSIRA
CNELGVAMVFTGVRHFLH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=477, Percent_Identity=37.3165618448637, Blast_Score=237, Evalue=2e-62,
Organism=Escherichia coli, GI1790439, Length=524, Percent_Identity=47.1374045801527, Blast_Score=431, Evalue=1e-122,
Organism=Caenorhabditis elegans, GI71985564, Length=477, Percent_Identity=36.2683438155136, Blast_Score=242, Evalue=3e-64,
Organism=Caenorhabditis elegans, GI71985574, Length=307, Percent_Identity=30.9446254071661, Blast_Score=92, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323768, Length=473, Percent_Identity=36.7864693446089, Blast_Score=238, Evalue=2e-63,
Organism=Saccharomyces cerevisiae, GI6323056, Length=469, Percent_Identity=35.6076759061834, Blast_Score=229, Evalue=8e-61,
Organism=Drosophila melanogaster, GI24649832, Length=478, Percent_Identity=37.0292887029289, Blast_Score=227, Evalue=1e-59,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 53550; Mature: 53418

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPIALLSVSDKTGLVDFARALVTEFEYQLISSGGTARALAEAGVPVQEVGDFTGAAEMLG
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHCCCCHHHHHC
GRVKTLHPKIHGGILARRDQESDRRDLETHSIAPIDLVVVNLYPFEQDPRIEQIDVGGPT
CCEEEECCHHHCCEEEECCCCHHHHHCHHCCCCCEEEEEEEEECCCCCCCCEEEECCCCH
MVRAAAKNHAFVTVLVSPGQYEGCLAELRRNRGVTTLPFRAACAAAAFARTAAYDRAIAA
HHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHH
WFAGSEPVLGERLVLELERVQPLRYGENPHQAAAWYRHGPAAGWSTGRILQGKALSFNNL
HHCCCCCCCCHHHHHHHHHCCCHHCCCCHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCE
IDLEASRRLVAEFDDTPAAVIIKHTNPCGVAADATIAAAYRRAFDADSASAFGGIVALNR
EECHHHCHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHCCCEEEECC
PCDEPTAALLGQTFLECVVAPAFTLGALALLERKKNLRLLELADLTTPPVFDVKPISGGF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCE
LIQQVDAPVRAEDWKVVTEAVPDEDQRAELLFAWKVCQHVKSNAIVVSHLRQTLGVGAGQ
EEEECCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
MNRVGSARLALAQAGEKARGAVLASDGFFPFDDTVRAAAERGIRAIVQPGGSLRDEDSIR
HHHCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCHHHH
ACNELGVAMVFTGVRHFLH
HHHHCCHHHHHHHHHHHCC
>Mature Secondary Structure 
PIALLSVSDKTGLVDFARALVTEFEYQLISSGGTARALAEAGVPVQEVGDFTGAAEMLG
CEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHCCCCHHHHHC
GRVKTLHPKIHGGILARRDQESDRRDLETHSIAPIDLVVVNLYPFEQDPRIEQIDVGGPT
CCEEEECCHHHCCEEEECCCCHHHHHCHHCCCCCEEEEEEEEECCCCCCCCEEEECCCCH
MVRAAAKNHAFVTVLVSPGQYEGCLAELRRNRGVTTLPFRAACAAAAFARTAAYDRAIAA
HHHHHCCCCEEEEEEECCCCCHHHHHHHHHCCCCEECCHHHHHHHHHHHHHHHHHHHHHH
WFAGSEPVLGERLVLELERVQPLRYGENPHQAAAWYRHGPAAGWSTGRILQGKALSFNNL
HHCCCCCCCCHHHHHHHHHCCCHHCCCCHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCE
IDLEASRRLVAEFDDTPAAVIIKHTNPCGVAADATIAAAYRRAFDADSASAFGGIVALNR
EECHHHCHHHHHCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCHHCCCEEEECC
PCDEPTAALLGQTFLECVVAPAFTLGALALLERKKNLRLLELADLTTPPVFDVKPISGGF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEECCCCE
LIQQVDAPVRAEDWKVVTEAVPDEDQRAELLFAWKVCQHVKSNAIVVSHLRQTLGVGAGQ
EEEECCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCC
MNRVGSARLALAQAGEKARGAVLASDGFFPFDDTVRAAAERGIRAIVQPGGSLRDEDSIR
HHHCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHCCCEEEECCCCCCCCCHHHH
ACNELGVAMVFTGVRHFLH
HHHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA