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The map label for this gene is sphS [H]

Identifier: 37519987

GI number: 37519987

Start: 439564

End: 440721

Strand: Direct

Name: sphS [H]

Synonym: glr0418

Alternate gene names: 37519987

Gene position: 439564-440721 (Clockwise)

Preceding gene: 37519982

Following gene: 37519988

Centisome position: 9.43

GC content: 67.18

Gene sequence:

>1158_bases
GTGTCCCAAGGGGAGCGTTGCCACAATAGGGGCGGAAGGCACGCGAGGGGAGAAGGCGCCGTGTGGCCCTGGCTGGCTGT
GGGTATTGCCTCGGGAGCACTGCTGGGCGCTCTGGGCACCTGGCTCGCTCTGCGCGGGTCGATCGCCATCTATCGCGCTT
TGCTGGAAGCCGCCCCCGTCGCCTACGTTCTGCTCGATAGCCAAAACCGGCTCATCGCCGCCAGCTCCCTGGCCGGTCGC
TACCTGGAATTGCCGGCCATCGGTTTACCCGTGGTCGAACTGGACGCCACCTTGATGGACCTGGTGCTGCAGGCGCGCCG
GGGCATGACGGTACAGCAGCGCAACGTCGGCCTCAAGACCGTCCGCGGCGACTGGATGGTGGGTGCGCTGGCGGTACCGC
TGCCGGGTCTGCGGGTGGGACTCTACCTTGACGATCAAAGTGCGGTGGCCCAACTCCTTCAGCAGCGCGACCGCTGGGTG
GGAGACGCCGCCCACGAACTGAAGACCCCGCTCACCTCCATCCGGCTGGTGGCCGAGATGGTCCTGCCGCGGGTGGGGCC
GGATCAGCAGCGCTGGCTCGAGCGGCTACTGGGCGAGGTGCAGCGCCTCAATCTGTTGGTGCAGGATCTGCTCGAATTTA
GCCGCTGGCAGGCGGGCCGCACCCAGTTGAATTTCAAAGAAGGGGTGGATCTGGTGCAACTTGTCGAGCAGACCTGGGCC
ACCCTGGAGCCGCTCGCCGAGCGCCGCCAGGTGCGCATAGAGATCGACAGCCCCGCGAGCGTGAACGTCTGTGCCGATGA
AGAGCGCCTCTACCGGGCCTTGCTCAACCTGCTCGACAACGCCGTGCGCTACAGCCCTAAGGGCGAAGCGTTCTGGGTGC
GCATGCGCTCACAGCCCGATTGGGTAACCATCGAGATTATCGACGGCGGCCCCGGTTTTCCGCGTCAGGATCTCGATCGG
GTCTTCGAGCGCTTTTACCGCGCCGATCCGGCCCGCGCCCGCCAGACCGGCGGTACCGGCCTGGGGCTCGCCATCGTCCG
TCAGATTGTCGAAGCGCACCAGGGCATAGTCAAAGCCGACAACCACCCCGAGACCGGCGGCGCCTGGCTGAGCGTGCGGC
TGCCGGTGCGGCCGGTGCCCACCGCCACCGTCCGCTGA

Upstream 100 bases:

>100_bases
GCGGCACCAACAACTGGCCGATCGCTTCTTTTAAGTTCAGTTGTGCGGCGCCCGGTAAATTGGCCATTCCGACCTGCGAC
CCATCGACGGCCCATCGATT

Downstream 100 bases:

>100_bases
AACTATCTGCCTTGGGGCGTAGACGATGTTCGACTGATTTGAAGAGCGGCCGGGCGGGGCGGCCAACCTAAAGTGAAGCG
GAAGCCATATCTACTTGAAA

Product: two-component sensor histidine kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MSQGERCHNRGGRHARGEGAVWPWLAVGIASGALLGALGTWLALRGSIAIYRALLEAAPVAYVLLDSQNRLIAASSLAGR
YLELPAIGLPVVELDATLMDLVLQARRGMTVQQRNVGLKTVRGDWMVGALAVPLPGLRVGLYLDDQSAVAQLLQQRDRWV
GDAAHELKTPLTSIRLVAEMVLPRVGPDQQRWLERLLGEVQRLNLLVQDLLEFSRWQAGRTQLNFKEGVDLVQLVEQTWA
TLEPLAERRQVRIEIDSPASVNVCADEERLYRALLNLLDNAVRYSPKGEAFWVRMRSQPDWVTIEIIDGGPGFPRQDLDR
VFERFYRADPARARQTGGTGLGLAIVRQIVEAHQGIVKADNHPETGGAWLSVRLPVRPVPTATVR

Sequences:

>Translated_385_residues
MSQGERCHNRGGRHARGEGAVWPWLAVGIASGALLGALGTWLALRGSIAIYRALLEAAPVAYVLLDSQNRLIAASSLAGR
YLELPAIGLPVVELDATLMDLVLQARRGMTVQQRNVGLKTVRGDWMVGALAVPLPGLRVGLYLDDQSAVAQLLQQRDRWV
GDAAHELKTPLTSIRLVAEMVLPRVGPDQQRWLERLLGEVQRLNLLVQDLLEFSRWQAGRTQLNFKEGVDLVQLVEQTWA
TLEPLAERRQVRIEIDSPASVNVCADEERLYRALLNLLDNAVRYSPKGEAFWVRMRSQPDWVTIEIIDGGPGFPRQDLDR
VFERFYRADPARARQTGGTGLGLAIVRQIVEAHQGIVKADNHPETGGAWLSVRLPVRPVPTATVR
>Mature_384_residues
SQGERCHNRGGRHARGEGAVWPWLAVGIASGALLGALGTWLALRGSIAIYRALLEAAPVAYVLLDSQNRLIAASSLAGRY
LELPAIGLPVVELDATLMDLVLQARRGMTVQQRNVGLKTVRGDWMVGALAVPLPGLRVGLYLDDQSAVAQLLQQRDRWVG
DAAHELKTPLTSIRLVAEMVLPRVGPDQQRWLERLLGEVQRLNLLVQDLLEFSRWQAGRTQLNFKEGVDLVQLVEQTWAT
LEPLAERRQVRIEIDSPASVNVCADEERLYRALLNLLDNAVRYSPKGEAFWVRMRSQPDWVTIEIIDGGPGFPRQDLDRV
FERFYRADPARARQTGGTGLGLAIVRQIVEAHQGIVKADNHPETGGAWLSVRLPVRPVPTATVR

Specific function: Member of the two-component regulatory system sphR/sphS. Sensory kinase. Is involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to phos

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1786600, Length=214, Percent_Identity=35.0467289719626, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1788393, Length=230, Percent_Identity=33.4782608695652, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1790346, Length=231, Percent_Identity=34.1991341991342, Blast_Score=113, Evalue=2e-26,
Organism=Escherichia coli, GI1786783, Length=232, Percent_Identity=33.6206896551724, Blast_Score=112, Evalue=5e-26,
Organism=Escherichia coli, GI1790861, Length=272, Percent_Identity=30.8823529411765, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI145693157, Length=237, Percent_Identity=28.2700421940928, Blast_Score=95, Evalue=8e-21,
Organism=Escherichia coli, GI1786912, Length=230, Percent_Identity=31.304347826087, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787894, Length=237, Percent_Identity=31.2236286919831, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789149, Length=233, Percent_Identity=26.6094420600858, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1788549, Length=263, Percent_Identity=30.4182509505703, Blast_Score=89, Evalue=6e-19,
Organism=Escherichia coli, GI87082128, Length=210, Percent_Identity=30.4761904761905, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1790551, Length=218, Percent_Identity=28.4403669724771, Blast_Score=85, Evalue=9e-18,
Organism=Escherichia coli, GI1790436, Length=217, Percent_Identity=29.0322580645161, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789808, Length=242, Percent_Identity=30.9917355371901, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI48994928, Length=240, Percent_Identity=25.4166666666667, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788279, Length=235, Percent_Identity=25.531914893617, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI87081816, Length=240, Percent_Identity=28.3333333333333, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI1788713, Length=230, Percent_Identity=24.3478260869565, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1789403, Length=221, Percent_Identity=31.2217194570136, Blast_Score=72, Evalue=7e-14,
Organism=Escherichia coli, GI1790300, Length=247, Percent_Identity=27.9352226720648, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 42671; Mature: 42540

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS50109 HIS_KIN ; PS00226 IF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQGERCHNRGGRHARGEGAVWPWLAVGIASGALLGALGTWLALRGSIAIYRALLEAAPV
CCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
AYVLLDSQNRLIAASSLAGRYLELPAIGLPVVELDATLMDLVLQARRGMTVQQRNVGLKT
EEEEECCCCCEEEEHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHCCCCEE
VRGDWMVGALAVPLPGLRVGLYLDDQSAVAQLLQQRDRWVGDAAHELKTPLTSIRLVAEM
ECCCCEEEHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
VLPRVGPDQQRWLERLLGEVQRLNLLVQDLLEFSRWQAGRTQLNFKEGVDLVQLVEQTWA
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHH
TLEPLAERRQVRIEIDSPASVNVCADEERLYRALLNLLDNAVRYSPKGEAFWVRMRSQPD
HHHHHHCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
WVTIEIIDGGPGFPRQDLDRVFERFYRADPARARQTGGTGLGLAIVRQIVEAHQGIVKAD
EEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEECC
NHPETGGAWLSVRLPVRPVPTATVR
CCCCCCCEEEEEEECCCCCCCCCCC
>Mature Secondary Structure 
SQGERCHNRGGRHARGEGAVWPWLAVGIASGALLGALGTWLALRGSIAIYRALLEAAPV
CCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCE
AYVLLDSQNRLIAASSLAGRYLELPAIGLPVVELDATLMDLVLQARRGMTVQQRNVGLKT
EEEEECCCCCEEEEHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHCCCEEEHHCCCCEE
VRGDWMVGALAVPLPGLRVGLYLDDQSAVAQLLQQRDRWVGDAAHELKTPLTSIRLVAEM
ECCCCEEEHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
VLPRVGPDQQRWLERLLGEVQRLNLLVQDLLEFSRWQAGRTQLNFKEGVDLVQLVEQTWA
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCHHHHHHHHHHH
TLEPLAERRQVRIEIDSPASVNVCADEERLYRALLNLLDNAVRYSPKGEAFWVRMRSQPD
HHHHHHCCCEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
WVTIEIIDGGPGFPRQDLDRVFERFYRADPARARQTGGTGLGLAIVRQIVEAHQGIVKAD
EEEEEEEECCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCHHHHHHHHHHHHHCCCEECC
NHPETGGAWLSVRLPVRPVPTATVR
CCCCCCCEEEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8497200 [H]