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The map label for this gene is hisH

Identifier: 37519976

GI number: 37519976

Start: 427910

End: 428590

Strand: Direct

Name: hisH

Synonym: gvip039

Alternate gene names: 37519976

Gene position: 427910-428590 (Clockwise)

Preceding gene: 37519975

Following gene: 37519980

Centisome position: 9.18

GC content: 66.23

Gene sequence:

>681_bases
ATGGCGCGCACCCCAAACATTGCCCTGGTGGATTACGGCGTCGGCAACCTGCACTCGGCCCGCAAGGGCCTGGAGGCGAT
GGGAGCGCGGGTGACACTCAGCGGCCAGCCCCTGACGCTGAGCGCCGCCGACGGCGTCGTGCTGCCGGGGGTGGGCTCCT
TCGACACGGCGATTACCCGGCTCAACGACCGGGGGCTGGGAGATGCGATTATCCAACTGGTGCGCGCAGGACAGCCGATG
CTGGGCATCTGTCTGGGGCTGCAGGTGCTCTTTGACTCCTCTGAAGAAGGACGACTGCCGGGCCTGGGGCTGCTGCCCGG
TCGGGTGCGCCGCTTCCGGAGCGAACCCGGCCTCACCATTCCCCATGTGGGCTGGAACCAACTGCACTTTGACAATGTCG
ATTGCCCGCTGTGGCGGGATCTGGCGGCGGGCGGTTGGGTTTACTTTGTGCATTCTTATTACGTCGATCCTGCCCGCGCC
GAGGACCGCGCCGCTTCCGCCGTGCATGGCAGCCAGCACTTCACCGCCGCCGTGTGCCGGGACAACCTGATGGCGGTGCA
ATTTCACCCGGAAAAGTCAGCGGACACGGGACTGCGCATCCTCAAAAACTTTGTTGAGCGGGCCGCTTCGCGCAGCGCCG
CCGAAGTAGCGGCCCGCTGCGCTACTCGCCCTCCAGCTTGA

Upstream 100 bases:

>100_bases
TGGCAGGCGGTGGAGCAACGGCTCGGCGAGTGCCGCAAGACCCGCCGCGAGGTCTGCGAGGGCGGGATTTAAGATGGGAA
CAATTCTGGAGGACCCGGCG

Downstream 100 bases:

>100_bases
GGGTGGGCTCCGCCACCGTCCCGCCGATGTTTTTCAATTCGACAACGACGGCATCGGTGCTCTTCCAGCCCGCGGGCAGG
ACCACCTCGGCGCTCAGGGG

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MARTPNIALVDYGVGNLHSARKGLEAMGARVTLSGQPLTLSAADGVVLPGVGSFDTAITRLNDRGLGDAIIQLVRAGQPM
LGICLGLQVLFDSSEEGRLPGLGLLPGRVRRFRSEPGLTIPHVGWNQLHFDNVDCPLWRDLAAGGWVYFVHSYYVDPARA
EDRAASAVHGSQHFTAAVCRDNLMAVQFHPEKSADTGLRILKNFVERAASRSAAEVAARCATRPPA

Sequences:

>Translated_226_residues
MARTPNIALVDYGVGNLHSARKGLEAMGARVTLSGQPLTLSAADGVVLPGVGSFDTAITRLNDRGLGDAIIQLVRAGQPM
LGICLGLQVLFDSSEEGRLPGLGLLPGRVRRFRSEPGLTIPHVGWNQLHFDNVDCPLWRDLAAGGWVYFVHSYYVDPARA
EDRAASAVHGSQHFTAAVCRDNLMAVQFHPEKSADTGLRILKNFVERAASRSAAEVAARCATRPPA
>Mature_225_residues
ARTPNIALVDYGVGNLHSARKGLEAMGARVTLSGQPLTLSAADGVVLPGVGSFDTAITRLNDRGLGDAIIQLVRAGQPML
GICLGLQVLFDSSEEGRLPGLGLLPGRVRRFRSEPGLTIPHVGWNQLHFDNVDCPLWRDLAAGGWVYFVHSYYVDPARAE
DRAASAVHGSQHFTAAVCRDNLMAVQFHPEKSADTGLRILKNFVERAASRSAAEVAARCATRPPA

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=205, Percent_Identity=36.5853658536585, Blast_Score=118, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6319725, Length=217, Percent_Identity=32.258064516129, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_GLOVI (Q7NNK4)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923353.1
- ProteinModelPortal:   Q7NNK4
- SMR:   Q7NNK4
- GeneID:   2599711
- GenomeReviews:   BA000045_GR
- NMPDR:   fig|251221.1.peg.407
- HOGENOM:   HBG292341
- OMA:   SVRFAFE
- ProtClustDB:   PRK13141
- BioCyc:   GVIO251221:GLR0407-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 24220; Mature: 24089

Theoretical pI: Translated: 8.35; Mature: 8.35

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 84-84 ACT_SITE 189-189 ACT_SITE 191-191

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTPNIALVDYGVGNLHSARKGLEAMGARVTLSGQPLTLSAADGVVLPGVGSFDTAITR
CCCCCCEEEEECCCCCHHHHHHHHHHHCCEEEECCCCEEEECCCCEEECCCCCHHHHHHH
LNDRGLGDAIIQLVRAGQPMLGICLGLQVLFDSSEEGRLPGLGLLPGRVRRFRSEPGLTI
HCCCCHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHCCCCCCC
PHVGWNQLHFDNVDCPLWRDLAAGGWVYFVHSYYVDPARAEDRAASAVHGSQHFTAAVCR
CCCCCCCEEECCCCCCHHHHHHCCCEEEEEEHHHCCCCCHHHHHHHHHCCCCHHHHHHHC
DNLMAVQFHPEKSADTGLRILKNFVERAASRSAAEVAARCATRPPA
CCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCC
>Mature Secondary Structure 
ARTPNIALVDYGVGNLHSARKGLEAMGARVTLSGQPLTLSAADGVVLPGVGSFDTAITR
CCCCCEEEEECCCCCHHHHHHHHHHHCCEEEECCCCEEEECCCCEEECCCCCHHHHHHH
LNDRGLGDAIIQLVRAGQPMLGICLGLQVLFDSSEEGRLPGLGLLPGRVRRFRSEPGLTI
HCCCCHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHCCCCCCC
PHVGWNQLHFDNVDCPLWRDLAAGGWVYFVHSYYVDPARAEDRAASAVHGSQHFTAAVCR
CCCCCCCEEECCCCCCHHHHHHCCCEEEEEEHHHCCCCCHHHHHHHHHCCCCHHHHHHHC
DNLMAVQFHPEKSADTGLRILKNFVERAASRSAAEVAARCATRPPA
CCEEEEEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292