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The map label for this gene is ddl

Identifier: 37519866

GI number: 37519866

Start: 305135

End: 306043

Strand: Reverse

Name: ddl

Synonym: gll0297

Alternate gene names: 37519866

Gene position: 306043-305135 (Counterclockwise)

Preceding gene: 37519867

Following gene: 37519863

Centisome position: 6.57

GC content: 64.58

Gene sequence:

>909_bases
ATGCGCATCACGGTACTGTCGGGAAGCGATTCGCCCGAGCGGGAGGTGTCGCTGGTGAGCGGCCGGGCCGTGCAAGCGGC
TCTCGAAACGTTGGGCCATGAAGTGACCATGGTCGATCCCGGCCCGGATCTGCCGGTGCGCCTATTGGAAAACCCGCCCG
ATTTTGTCTGGATTGCGCTGCACGGCGACAAAGGCGAAAACGGCAAATTGCAGGGGTTGCTCGACTGGCTGGGCTTGCCC
TACAACGGTTCGGGGGTAACGGCGAGTGCCATTGCCATGGACAAAGTCGTCTCCAAGCGGTTGTTCGTCGCCGAGGGCAT
CCCCACCCCCGCCTACCGGGTGGCCGAAGCGGTGCCGCAGGTGAGCGAGTGTCAGGCGTGGTTGGCGGCTTTGGGATCGC
CGGTGGTGGTCAAACCCGCGGACGGCGGTTCGACGGTTGGGGTGACGATCGCCCGTGAGGCCGGGCACCTCCCTGAGGCG
GTGCGTCTGGCCCTGCAGTATTCGCCCCAGGTGCTCATCGAGCAGTATATTCCAGGGCAGGAAATCACCGTGGCACTACT
TGACGGGTTGGTGCTGCCTGCGATCGAAATCGTTCCGCAGGGGCGCGATTTTTATGACTACGAGGCGAAGTACGCCCCGG
GCGGCTCGCGCCATCTGATTCCCCCCAATCTCGCAGCCGATGTGCTCAAGGCGAGCGTCGATGCCGCCTACCGCGCCTGC
CGGGCGGTGGGCAGCACAGGCCTGGTGCGCGCCGATGTGCGCGTCGATCCTGAAGGGCGGCCGTGGGTGCTCGAAGTCAA
TACCCTGCCGGGCATGACCGCCACCTCTTTGGCGCCTGAGGCAGCCCAGGCGGCGGGCATCGATTTTGAGCAGTTGATTC
AGCGGATTATCGACCGTTCGCTGGATTGA

Upstream 100 bases:

>100_bases
CCGCCGCCGGCCGTTGGTCTAGTTCGCCCCTTCCAGTGCGACCCGACCGGCCCCGGTGCCGGAGAATTGTCATGTGCGAG
TGATGGGAGGAAGGGTGTAG

Downstream 100 bases:

>100_bases
GCGGATGGGACCTTGGCTTTCCATCACTTTGCTGCGTCCGGAAGCTTACGCCTGGGGTCTCTGTAGGCGCAACCGCAAGA
ACATCGTTCCCGCAACGGGG

Product: D-alanine--D-alanine ligase

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MRITVLSGSDSPEREVSLVSGRAVQAALETLGHEVTMVDPGPDLPVRLLENPPDFVWIALHGDKGENGKLQGLLDWLGLP
YNGSGVTASAIAMDKVVSKRLFVAEGIPTPAYRVAEAVPQVSECQAWLAALGSPVVVKPADGGSTVGVTIAREAGHLPEA
VRLALQYSPQVLIEQYIPGQEITVALLDGLVLPAIEIVPQGRDFYDYEAKYAPGGSRHLIPPNLAADVLKASVDAAYRAC
RAVGSTGLVRADVRVDPEGRPWVLEVNTLPGMTATSLAPEAAQAAGIDFEQLIQRIIDRSLD

Sequences:

>Translated_302_residues
MRITVLSGSDSPEREVSLVSGRAVQAALETLGHEVTMVDPGPDLPVRLLENPPDFVWIALHGDKGENGKLQGLLDWLGLP
YNGSGVTASAIAMDKVVSKRLFVAEGIPTPAYRVAEAVPQVSECQAWLAALGSPVVVKPADGGSTVGVTIAREAGHLPEA
VRLALQYSPQVLIEQYIPGQEITVALLDGLVLPAIEIVPQGRDFYDYEAKYAPGGSRHLIPPNLAADVLKASVDAAYRAC
RAVGSTGLVRADVRVDPEGRPWVLEVNTLPGMTATSLAPEAAQAAGIDFEQLIQRIIDRSLD
>Mature_302_residues
MRITVLSGSDSPEREVSLVSGRAVQAALETLGHEVTMVDPGPDLPVRLLENPPDFVWIALHGDKGENGKLQGLLDWLGLP
YNGSGVTASAIAMDKVVSKRLFVAEGIPTPAYRVAEAVPQVSECQAWLAALGSPVVVKPADGGSTVGVTIAREAGHLPEA
VRLALQYSPQVLIEQYIPGQEITVALLDGLVLPAIEIVPQGRDFYDYEAKYAPGGSRHLIPPNLAADVLKASVDAAYRAC
RAVGSTGLVRADVRVDPEGRPWVLEVNTLPGMTATSLAPEAAQAAGIDFEQLIQRIIDRSLD

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786280, Length=302, Percent_Identity=44.7019867549669, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1786579, Length=274, Percent_Identity=33.9416058394161, Blast_Score=138, Evalue=5e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_GLOVI (Q7NNW1)

Other databases:

- EMBL:   BA000045
- RefSeq:   NP_923243.1
- ProteinModelPortal:   Q7NNW1
- SMR:   Q7NNW1
- GeneID:   2599893
- GenomeReviews:   BA000045_GR
- KEGG:   gvi:gll0297
- NMPDR:   fig|251221.1.peg.297
- HOGENOM:   HBG427092
- OMA:   TDGWYDY
- ProtClustDB:   CLSK494701
- BioCyc:   GVIO251221:GLL0297-MONOMER
- BRENDA:   6.3.2.4
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 32020; Mature: 32020

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITVLSGSDSPEREVSLVSGRAVQAALETLGHEVTMVDPGPDLPVRLLENPPDFVWIAL
CEEEEECCCCCCCHHEEECCCHHHHHHHHHCCCEEEEECCCCCCCHHEECCCCCEEEEEE
HGDKGENGKLQGLLDWLGLPYNGSGVTASAIAMDKVVSKRLFVAEGIPTPAYRVAEAVPQ
ECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCC
VSECQAWLAALGSPVVVKPADGGSTVGVTIAREAGHLPEAVRLALQYSPQVLIEQYIPGQ
HHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHCCCCC
EITVALLDGLVLPAIEIVPQGRDFYDYEAKYAPGGSRHLIPPNLAADVLKASVDAAYRAC
HHHHHHHHHHHHHHHHEECCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RAVGSTGLVRADVRVDPEGRPWVLEVNTLPGMTATSLAPEAAQAAGIDFEQLIQRIIDRS
HHHCCCCEEEEEEEECCCCCCEEEEEECCCCCCHHHCCCHHHHHCCCCHHHHHHHHHHHC
LD
CC
>Mature Secondary Structure
MRITVLSGSDSPEREVSLVSGRAVQAALETLGHEVTMVDPGPDLPVRLLENPPDFVWIAL
CEEEEECCCCCCCHHEEECCCHHHHHHHHHCCCEEEEECCCCCCCHHEECCCCCEEEEEE
HGDKGENGKLQGLLDWLGLPYNGSGVTASAIAMDKVVSKRLFVAEGIPTPAYRVAEAVPQ
ECCCCCCCCHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCHHHHHHHCCC
VSECQAWLAALGSPVVVKPADGGSTVGVTIAREAGHLPEAVRLALQYSPQVLIEQYIPGQ
HHHHHHHHHHCCCCEEEEECCCCCEEEEEEECCCCCCHHHHHHHHHCCCHHHHHHCCCCC
EITVALLDGLVLPAIEIVPQGRDFYDYEAKYAPGGSRHLIPPNLAADVLKASVDAAYRAC
HHHHHHHHHHHHHHHHEECCCCCCCCCCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHH
RAVGSTGLVRADVRVDPEGRPWVLEVNTLPGMTATSLAPEAAQAAGIDFEQLIQRIIDRS
HHHCCCCEEEEEEEECCCCCCEEEEEECCCCCCHHHCCCHHHHHCCCCHHHHHHHHHHHC
LD
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292