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The map label for this gene is topA [H]

Identifier: 34499724

GI number: 34499724

Start: 4602812

End: 4605121

Strand: Direct

Name: topA [H]

Synonym: CV_4269

Alternate gene names: 34499724

Gene position: 4602812-4605121 (Clockwise)

Preceding gene: 34499723

Following gene: 34499726

Centisome position: 96.88

GC content: 64.37

Gene sequence:

>2310_bases
ATGCCCACCAGCCTCCTGATCGTCGAGTCGCCATCCAAGGCGAAGACGCTGAAAAAATACCTGGGCCCGGACTTCGAGGT
CCTGGCCTCCTACGGCCACGTGCGCGACCTGGTGCCGAAGAACGGCGCCGTCGATCCGGAAAAAGACTTCGCGATGAAGT
ACCAGCTGATCGCGCGCAACAGCAAGCACGTCGACGCCATCGTCAGCGCGGTGCGCGAGGCCGACCACGTTTACCTGGCG
ACTGACCCGGACCGCGAAGGCGAAGCCATTTCCTGGCACCTGGTGCAGATCCTCAACAGCAAGAAGCTGCTGAAGGACAA
GACCGCCCAGCGCGTGGTGTTCCACGAGATCACCAAGAACGCGGTGCTGGACGCGATCCAGAACCCGCGCGACATCGCGC
AGGACCTGGTGGACGCGCAGCAGGCGCGCCGCGCGCTGGACTACCTGGTGGGCTTCAACCTGTCGCCGCTGTTGTGGAAG
AAGATCCGCCGCGGCCTGTCCGCCGGCCGCGTGCAGAGCCCGGCCTTGCGCCTGATCTGCGAACGCGAGAACGAGATCAA
GGCCTTCGTGCAGCAGGAATACTGGTCGGTCCACCTGGACAGCCACAAGAGCCGCACCAAGTTCAGCGCCAAACTGACCA
CGCTCGGCGGCAAGAAACTGGACCAGTTCGACATCCCGAACGAGGCCGAACAGGCGTCGGTGCTGGCCCGCCTGCAGGGC
CTGCCGGCCACCGTGGGCAGCATCGAGAAGAAAAAGAAATCGCGCAGCCCGGCCGCGCCGTTCACCACCTCCACGCTGCA
GCAGGAGGCCGTGCGCAAGCTCGGCATGACCACCGACCGCGCGATGCGCACCGCGCAGCAGCTGTACGAGGGCATGGACA
TCGGCCAGGGCACCGTCGGCCTGATCACCTATATGCGTACCGACTCGGTGGCGCTGGCCAATGAAGCGGTGGAGGAAATC
CGCGGCTACATCGGCCTGAAATGGGACGCCGACTACCTGCCGAAGAACCCGGCGGTCTACAAGAGCAAGGCCAAGAACGC
CCAGGAGGCGCACGAAGCCATCCGCCCGACCTCCATCCTGCGCACGCCGGAGATGGTCAAGCCCTTCCTGACCACCGACC
AGTTCAAGCTGTACGACATGATCTGGAAGCGGACGCTGGCCTGCCAGATGGCGCCGGCCAAGTTCGACACCACCAGCGTC
GACATCATGGTCGGCGAGGGCGTGTTCCGCGCCAGCGGCCAGGTGCAGACCTTCGCCGGCTTCCTCGCCGTGTACGAGGA
GGATGTCGACGATGCCGAGGACGAAGACAACGCCAAGCTGCCGCTGCTGACCGAGGGCGAGGATCTGCCGGTGGACAAGC
TGTACGGCGAGCAGCACTTCACCCAGCCGCCGCCGCGCTTCTCCGAAGCCAGCCTGGTGAAGGCGCTGGAAGAATTCGGC
ATCGGCCGTCCGTCCACCTACGCCAGCATCATCTCGACGCTGAAGGATCGCGAGTACGTGATCCTGGACAAGAAGCGCTT
CCTGCCGACCGATACCGGCGACATCGTCAACAAGTTCCTGACCGAGCACTTCGCCCAGTACGTCGACTACAACTTCACCG
CCAAGCTGGAAAACCAGCTGGACGAGATCGCCAACGGCGGCCGCCAATGGGTGCCGGTGATGGACACCTTCTGGAAGGGC
TTCAAGAAGCAGATCGACGAGAAGGAAAGCATCTCCCGCGCCGAAGTCACCACCGAAAGCCTGGACGAGGACTGCCCGAA
ATGCGGCAAGCCGCTGTCGATCAAGTTCGGCAAGCGCGGCCGCTTCATCGCCTGCACCGGCTACCCGGAATGCGATTACA
CCCGCAACATGGGCGAAACCGCCGAGCAGGCGGCCGAGGAGGCGGAAGCGCCGACGGTGATCGAAGGCCGTTCCTGCCCG
GACTGCGGCGGCGAGCTGCACATCAAGAAAGGCCGCTACGGCAAATTCATCGGCTGCGCCAACTATCCGAAGTGCAAGCA
CATCGAGCCTTTGGAGAAGCCGCGCGAAACCGGCGTCACCTGCCCGGAATGCAAGACCGGCCAGCTGATCGAGCGCAAGA
GCCGCTACGGCAAGCTGTTCTACAGCTGCAACACCTATCCGAAGTGCAAGTACGCGACCTGGAACCCGCCGATCGCCGAG
CCGTGCCCGCAGTGCAACTGGCCGATCCTGACGATCAAGACCACCAAGCGCCGCGGCACCGAGAAGGTGTGCCCGCAGAA
GGAATGCGGCTACGCCGAGCAGATCGCGCCGCCGGAAGGCAAGGACGCCGCGCCCGCCGAAGAGGCGTAA

Upstream 100 bases:

>100_bases
CTTTCCGTGATCAAGAGCGCGCTTGCGCTCTTGTTTTGTTTGACGGATATAACAAGATGTGTTCGCCGGAGACCCCGGCA
TCGAATATTGAAAAGACAAC

Downstream 100 bases:

>100_bases
GCCCGGGGCTCCCGAACCGATCAACAAACAGCCCTGCCGGCGTTGCCGACAGGGCTGTTTTTTCGCCATATTCAAAGCCT
AGGCAGAGATATCCCGCCTA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 769; Mature: 768

Protein sequence:

>769_residues
MPTSLLIVESPSKAKTLKKYLGPDFEVLASYGHVRDLVPKNGAVDPEKDFAMKYQLIARNSKHVDAIVSAVREADHVYLA
TDPDREGEAISWHLVQILNSKKLLKDKTAQRVVFHEITKNAVLDAIQNPRDIAQDLVDAQQARRALDYLVGFNLSPLLWK
KIRRGLSAGRVQSPALRLICERENEIKAFVQQEYWSVHLDSHKSRTKFSAKLTTLGGKKLDQFDIPNEAEQASVLARLQG
LPATVGSIEKKKKSRSPAAPFTTSTLQQEAVRKLGMTTDRAMRTAQQLYEGMDIGQGTVGLITYMRTDSVALANEAVEEI
RGYIGLKWDADYLPKNPAVYKSKAKNAQEAHEAIRPTSILRTPEMVKPFLTTDQFKLYDMIWKRTLACQMAPAKFDTTSV
DIMVGEGVFRASGQVQTFAGFLAVYEEDVDDAEDEDNAKLPLLTEGEDLPVDKLYGEQHFTQPPPRFSEASLVKALEEFG
IGRPSTYASIISTLKDREYVILDKKRFLPTDTGDIVNKFLTEHFAQYVDYNFTAKLENQLDEIANGGRQWVPVMDTFWKG
FKKQIDEKESISRAEVTTESLDEDCPKCGKPLSIKFGKRGRFIACTGYPECDYTRNMGETAEQAAEEAEAPTVIEGRSCP
DCGGELHIKKGRYGKFIGCANYPKCKHIEPLEKPRETGVTCPECKTGQLIERKSRYGKLFYSCNTYPKCKYATWNPPIAE
PCPQCNWPILTIKTTKRRGTEKVCPQKECGYAEQIAPPEGKDAAPAEEA

Sequences:

>Translated_769_residues
MPTSLLIVESPSKAKTLKKYLGPDFEVLASYGHVRDLVPKNGAVDPEKDFAMKYQLIARNSKHVDAIVSAVREADHVYLA
TDPDREGEAISWHLVQILNSKKLLKDKTAQRVVFHEITKNAVLDAIQNPRDIAQDLVDAQQARRALDYLVGFNLSPLLWK
KIRRGLSAGRVQSPALRLICERENEIKAFVQQEYWSVHLDSHKSRTKFSAKLTTLGGKKLDQFDIPNEAEQASVLARLQG
LPATVGSIEKKKKSRSPAAPFTTSTLQQEAVRKLGMTTDRAMRTAQQLYEGMDIGQGTVGLITYMRTDSVALANEAVEEI
RGYIGLKWDADYLPKNPAVYKSKAKNAQEAHEAIRPTSILRTPEMVKPFLTTDQFKLYDMIWKRTLACQMAPAKFDTTSV
DIMVGEGVFRASGQVQTFAGFLAVYEEDVDDAEDEDNAKLPLLTEGEDLPVDKLYGEQHFTQPPPRFSEASLVKALEEFG
IGRPSTYASIISTLKDREYVILDKKRFLPTDTGDIVNKFLTEHFAQYVDYNFTAKLENQLDEIANGGRQWVPVMDTFWKG
FKKQIDEKESISRAEVTTESLDEDCPKCGKPLSIKFGKRGRFIACTGYPECDYTRNMGETAEQAAEEAEAPTVIEGRSCP
DCGGELHIKKGRYGKFIGCANYPKCKHIEPLEKPRETGVTCPECKTGQLIERKSRYGKLFYSCNTYPKCKYATWNPPIAE
PCPQCNWPILTIKTTKRRGTEKVCPQKECGYAEQIAPPEGKDAAPAEEA
>Mature_768_residues
PTSLLIVESPSKAKTLKKYLGPDFEVLASYGHVRDLVPKNGAVDPEKDFAMKYQLIARNSKHVDAIVSAVREADHVYLAT
DPDREGEAISWHLVQILNSKKLLKDKTAQRVVFHEITKNAVLDAIQNPRDIAQDLVDAQQARRALDYLVGFNLSPLLWKK
IRRGLSAGRVQSPALRLICERENEIKAFVQQEYWSVHLDSHKSRTKFSAKLTTLGGKKLDQFDIPNEAEQASVLARLQGL
PATVGSIEKKKKSRSPAAPFTTSTLQQEAVRKLGMTTDRAMRTAQQLYEGMDIGQGTVGLITYMRTDSVALANEAVEEIR
GYIGLKWDADYLPKNPAVYKSKAKNAQEAHEAIRPTSILRTPEMVKPFLTTDQFKLYDMIWKRTLACQMAPAKFDTTSVD
IMVGEGVFRASGQVQTFAGFLAVYEEDVDDAEDEDNAKLPLLTEGEDLPVDKLYGEQHFTQPPPRFSEASLVKALEEFGI
GRPSTYASIISTLKDREYVILDKKRFLPTDTGDIVNKFLTEHFAQYVDYNFTAKLENQLDEIANGGRQWVPVMDTFWKGF
KKQIDEKESISRAEVTTESLDEDCPKCGKPLSIKFGKRGRFIACTGYPECDYTRNMGETAEQAAEEAEAPTVIEGRSCPD
CGGELHIKKGRYGKFIGCANYPKCKHIEPLEKPRETGVTCPECKTGQLIERKSRYGKLFYSCNTYPKCKYATWNPPIAEP
CPQCNWPILTIKTTKRRGTEKVCPQKECGYAEQIAPPEGKDAAPAEEA

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=616, Percent_Identity=26.1363636363636, Blast_Score=137, Evalue=3e-32,
Organism=Homo sapiens, GI4507635, Length=682, Percent_Identity=23.4604105571848, Blast_Score=121, Evalue=3e-27,
Organism=Escherichia coli, GI1787529, Length=822, Percent_Identity=37.956204379562, Blast_Score=497, Evalue=1e-141,
Organism=Escherichia coli, GI48994931, Length=180, Percent_Identity=45, Blast_Score=180, Evalue=3e-46,
Organism=Escherichia coli, GI1788061, Length=565, Percent_Identity=26.0176991150442, Blast_Score=132, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI17555378, Length=506, Percent_Identity=27.4703557312253, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI32563869, Length=702, Percent_Identity=23.9316239316239, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6323263, Length=499, Percent_Identity=23.6472945891784, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585251, Length=682, Percent_Identity=24.1935483870968, Blast_Score=121, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24640096, Length=668, Percent_Identity=22.4550898203593, Blast_Score=96, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 86216; Mature: 86085

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTSLLIVESPSKAKTLKKYLGPDFEVLASYGHVRDLVPKNGAVDPEKDFAMKYQLIARN
CCCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
SKHVDAIVSAVREADHVYLATDPDREGEAISWHLVQILNSKKLLKDKTAQRVVFHEITKN
CHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLDAIQNPRDIAQDLVDAQQARRALDYLVGFNLSPLLWKKIRRGLSAGRVQSPALRLIC
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHH
ERENEIKAFVQQEYWSVHLDSHKSRTKFSAKLTTLGGKKLDQFDIPNEAEQASVLARLQG
CCCHHHHHHHHHHHHEEEECCCCCHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
LPATVGSIEKKKKSRSPAAPFTTSTLQQEAVRKLGMTTDRAMRTAQQLYEGMDIGQGTVG
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEE
LITYMRTDSVALANEAVEEIRGYIGLKWDADYLPKNPAVYKSKAKNAQEAHEAIRPTSIL
EEEEECCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHCCHHHHHHHHCCHHHH
RTPEMVKPFLTTDQFKLYDMIWKRTLACQMAPAKFDTTSVDIMVGEGVFRASGQVQTFAG
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEECCCCCHHHHHH
FLAVYEEDVDDAEDEDNAKLPLLTEGEDLPVDKLYGEQHFTQPPPRFSEASLVKALEEFG
HHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
IGRPSTYASIISTLKDREYVILDKKRFLPTDTGDIVNKFLTEHFAQYVDYNFTAKLENQL
CCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
DEIANGGRQWVPVMDTFWKGFKKQIDEKESISRAEVTTESLDEDCPKCGKPLSIKFGKRG
HHHHCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
RFIACTGYPECDYTRNMGETAEQAAEEAEAPTVIEGRSCPDCGGELHIKKGRYGKFIGCA
CEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEECCCCCCCCCCEEEEECCCCCCEEECC
NYPKCKHIEPLEKPRETGVTCPECKTGQLIERKSRYGKLFYSCNTYPKCKYATWNPPIAE
CCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCEECCCCCCCCC
PCPQCNWPILTIKTTKRRGTEKVCPQKECGYAEQIAPPEGKDAAPAEEA
CCCCCCCCEEEEEECCCCCCHHCCCCHHCCCHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
PTSLLIVESPSKAKTLKKYLGPDFEVLASYGHVRDLVPKNGAVDPEKDFAMKYQLIARN
CCCEEEEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCC
SKHVDAIVSAVREADHVYLATDPDREGEAISWHLVQILNSKKLLKDKTAQRVVFHEITKN
CHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVLDAIQNPRDIAQDLVDAQQARRALDYLVGFNLSPLLWKKIRRGLSAGRVQSPALRLIC
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHHHHHH
ERENEIKAFVQQEYWSVHLDSHKSRTKFSAKLTTLGGKKLDQFDIPNEAEQASVLARLQG
CCCHHHHHHHHHHHHEEEECCCCCHHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHCC
LPATVGSIEKKKKSRSPAAPFTTSTLQQEAVRKLGMTTDRAMRTAQQLYEGMDIGQGTVG
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCEE
LITYMRTDSVALANEAVEEIRGYIGLKWDADYLPKNPAVYKSKAKNAQEAHEAIRPTSIL
EEEEECCCCHHHHHHHHHHHHHHCCCEECCCCCCCCCCHHHHHHCCHHHHHHHHCCHHHH
RTPEMVKPFLTTDQFKLYDMIWKRTLACQMAPAKFDTTSVDIMVGEGVFRASGQVQTFAG
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCEECCCCCHHHHHH
FLAVYEEDVDDAEDEDNAKLPLLTEGEDLPVDKLYGEQHFTQPPPRFSEASLVKALEEFG
HHHHHHHCCCCCCCCCCCCCCEEECCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHC
IGRPSTYASIISTLKDREYVILDKKRFLPTDTGDIVNKFLTEHFAQYVDYNFTAKLENQL
CCCCHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
DEIANGGRQWVPVMDTFWKGFKKQIDEKESISRAEVTTESLDEDCPKCGKPLSIKFGKRG
HHHHCCCCEEEHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCC
RFIACTGYPECDYTRNMGETAEQAAEEAEAPTVIEGRSCPDCGGELHIKKGRYGKFIGCA
CEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCEECCCCCCCCCCEEEEECCCCCCEEECC
NYPKCKHIEPLEKPRETGVTCPECKTGQLIERKSRYGKLFYSCNTYPKCKYATWNPPIAE
CCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHCEEEEECCCCCCCEECCCCCCCCC
PCPQCNWPILTIKTTKRRGTEKVCPQKECGYAEQIAPPEGKDAAPAEEA
CCCCCCCCEEEEEECCCCCCHHCCCCHHCCCHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]