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The map label for this gene is pyrE

Identifier: 34499703

GI number: 34499703

Start: 4584954

End: 4585598

Strand: Direct

Name: pyrE

Synonym: CV_4248

Alternate gene names: 34499703

Gene position: 4584954-4585598 (Clockwise)

Preceding gene: 34499700

Following gene: 34499704

Centisome position: 96.5

GC content: 65.27

Gene sequence:

>645_bases
ATGAGCGACTTTCGTCAGGATTTTATTCGTTTTGCGCTCGACAAGCAGGTGCTGAAATTTGGCGAGTTCATCACCAAGGC
GGGCCGCAAGTCGCCGTACTTCTTCAACGCCGGCCTGTTCAACGACGGCCTGTCCACGCTGAACCTGTCGCGATTCTACG
CCAAGTCCATCCAGCAAAGCGGCATCCAGTTCGACATGCTGTTCGGGCCGGCCTATAAAGGCATCATACTGGCCGCCGCC
GCCGGCATGGCGCTGGCCGAGCAGGGCCGCAACGTGCCGTTCGCCTACAACCGCAAGGAAGCCAAGGACCACGGCGAGGG
CGGCACCCTGGTCGGCGCGCCGCTGAAGGGCAAGGTGCTGATCATCGACGACGTGATCTCGGCCGGCACCTCGGTGCGCG
AGTCGGTGGAGCTGATCCGCGCCGCCGGCGCCGAACCGGCCGGCGTCGCCATCGCGCTGGACCGGATGGAGCGCGGCCAG
GGCGAATTGTCGGCGGTGCAGGAAGTGGCCCAGCAACATGGCCTGCCGGTGGTCGCCATCGCCACGCTGAAAGACCTGCT
GGGTTTCCTGGAAAACAGCCCGGAACTGGCCGCCCACCTCGACGCGGTGCGCGCCTACCGCGCCCAATACGGAGTCGACG
ACTGA

Upstream 100 bases:

>100_bases
AGATTGGCGGAAACGATTCGAAGCATTGTGTCTCCCGTGCTATGCTTGCGGCGGTTTTTTTCAGCCGTTCAAACAGTTAA
CGATTCGATAAGGAATGAGG

Downstream 100 bases:

>100_bases
CATGAAAGCGCTTGCCGGACTCACGCTGCTGTTGTGCTCGCTGGCCCAGGCCGGCGTCTACAAATGGGTGGACGAGGCCG
GCAACACGCATTACAGCGAC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 214; Mature: 213

Protein sequence:

>214_residues
MSDFRQDFIRFALDKQVLKFGEFITKAGRKSPYFFNAGLFNDGLSTLNLSRFYAKSIQQSGIQFDMLFGPAYKGIILAAA
AGMALAEQGRNVPFAYNRKEAKDHGEGGTLVGAPLKGKVLIIDDVISAGTSVRESVELIRAAGAEPAGVAIALDRMERGQ
GELSAVQEVAQQHGLPVVAIATLKDLLGFLENSPELAAHLDAVRAYRAQYGVDD

Sequences:

>Translated_214_residues
MSDFRQDFIRFALDKQVLKFGEFITKAGRKSPYFFNAGLFNDGLSTLNLSRFYAKSIQQSGIQFDMLFGPAYKGIILAAA
AGMALAEQGRNVPFAYNRKEAKDHGEGGTLVGAPLKGKVLIIDDVISAGTSVRESVELIRAAGAEPAGVAIALDRMERGQ
GELSAVQEVAQQHGLPVVAIATLKDLLGFLENSPELAAHLDAVRAYRAQYGVDD
>Mature_213_residues
SDFRQDFIRFALDKQVLKFGEFITKAGRKSPYFFNAGLFNDGLSTLNLSRFYAKSIQQSGIQFDMLFGPAYKGIILAAAA
GMALAEQGRNVPFAYNRKEAKDHGEGGTLVGAPLKGKVLIIDDVISAGTSVRESVELIRAAGAEPAGVAIALDRMERGQG
ELSAVQEVAQQHGLPVVAIATLKDLLGFLENSPELAAHLDAVRAYRAQYGVDD

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=187, Percent_Identity=29.4117647058824, Blast_Score=84, Evalue=1e-16,
Organism=Escherichia coli, GI1790073, Length=213, Percent_Identity=59.6244131455399, Blast_Score=268, Evalue=2e-73,
Organism=Caenorhabditis elegans, GI17508631, Length=204, Percent_Identity=27.4509803921569, Blast_Score=63, Evalue=1e-10,
Organism=Saccharomyces cerevisiae, GI6323530, Length=220, Percent_Identity=45.4545454545455, Blast_Score=188, Evalue=7e-49,
Organism=Saccharomyces cerevisiae, GI6323927, Length=223, Percent_Identity=43.0493273542601, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_CHRVO (Q7NQ92)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903918.1
- ProteinModelPortal:   Q7NQ92
- SMR:   Q7NQ92
- GeneID:   2549706
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4248
- NMPDR:   fig|243365.1.peg.4248
- HOGENOM:   HBG404341
- OMA:   FAFNRKE
- PhylomeDB:   Q7NQ92
- ProtClustDB:   PRK00455
- BioCyc:   CVIO243365:CV_4248-MONOMER
- BRENDA:   2.4.2.10
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 23037; Mature: 22905

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 26-26 BINDING 98-98 BINDING 99-99 BINDING 102-102 BINDING 104-104 BINDING 127-127 BINDING 155-155

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFRQDFIRFALDKQVLKFGEFITKAGRKSPYFFNAGLFNDGLSTLNLSRFYAKSIQQS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHC
GIQFDMLFGPAYKGIILAAAAGMALAEQGRNVPFAYNRKEAKDHGEGGTLVGAPLKGKVL
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHCCCCCCCEEECCCCCCEE
IIDDVISAGTSVRESVELIRAAGAEPAGVAIALDRMERGQGELSAVQEVAQQHGLPVVAI
EEECHHHCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHCCCCHHHHHHHHHHHCCCCEEHH
ATLKDLLGFLENSPELAAHLDAVRAYRAQYGVDD
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDFRQDFIRFALDKQVLKFGEFITKAGRKSPYFFNAGLFNDGLSTLNLSRFYAKSIQQS
CHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHC
GIQFDMLFGPAYKGIILAAAAGMALAEQGRNVPFAYNRKEAKDHGEGGTLVGAPLKGKVL
CCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHCCCCCCCEEECCCCCCEE
IIDDVISAGTSVRESVELIRAAGAEPAGVAIALDRMERGQGELSAVQEVAQQHGLPVVAI
EEECHHHCCHHHHHHHHHHHHCCCCCCCCEEEHHHHHCCCCHHHHHHHHHHHCCCCEEHH
ATLKDLLGFLENSPELAAHLDAVRAYRAQYGVDD
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782