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The map label for this gene is miaB

Identifier: 34499604

GI number: 34499604

Start: 4489369

End: 4490703

Strand: Direct

Name: miaB

Synonym: CV_4149

Alternate gene names: 34499604

Gene position: 4489369-4490703 (Clockwise)

Preceding gene: 34499603

Following gene: 34499605

Centisome position: 94.49

GC content: 64.57

Gene sequence:

>1335_bases
ATGAAGAAGGTATACATCAAGACCTTCGGCTGCCAGATGAACGAGTACGATTCCGACAAGATGGCGGACGTGCTGGGCAG
CGCCGAAGGCATGGTCAAGACGGACAACCCGGAAGAAGCCGACGTCATCCTGTTCAACACCTGTAGCGTGCGGGAGAAGG
CCCAGGAGAAAGTGTTCTCCGACCTCGGCCGCATCCGCCCGCTGAAGGAAGCCAATCCCGACCTGATCATCGGCGTCGGC
GGCTGCGTGGCCAGCCAGGAAGGCGACGCCATCGTCAAGCGCGCGCCCTTCGTCGACGTGGTGTTCGGCCCGCAGACCCT
GCACCGGCTGCCCGACCTGATCGAGAGCCGCAAACAGAGCGGCCGCTCGCAAGTGGACATCTCCTTCCCGGAGATCGAAA
AGTTCGACCACATCCCGCCGGCCAAGGTGGATGGCGGCGCGGCCTTCGTCTCGATCATGGAAGGCTGTTCCAAGTACTGC
TCGTTCTGCGTGGTGCCCTATACCCGCGGCGAGGAAGTGTCGCGTCCGTTCGAGGACGTGCTGACGGAGATCGCCGGCCT
GGCCGCCCAGGGCGTCAAGGAAATCACGCTGCTCGGCCAGAACGTCAACGCCTACCGCGGCCTGATGAGCGACGGCGAGA
TCGCCGACTTCGCGCTGCTCTTGGAATACGTGCACGAAGTGCCTGGCGTGGAGCGCATCCGCTTCACCACCAGCCACCCG
CGCGAATTCTCCCAGCGCATCATCGACTGCTACGCCAAGCTGCCCAAGCTGGTCTCCCACCTGCACCTGCCGGTGCAGAG
CGGCTCCGACCGCGTGCTGATGGCGATGAAGCGCGGCTACACCGGCCTGGAGTACAAGTCCATCATCCGCAAGCTGCGCG
CGATCCGCCCCGACCTGTGCCTGTCGTCCGACTTCATCATCGGCTTCCCGGGCGAGACCGAGGCCGACTTCGAGCAGACG
CTGAAGCTGGTGCGCGACTGCGAGTTCGACTTCAGCTTCGTGTTCATCTACAGCCCGCGCCCGGGCACGCCGGCGGCCAA
TCTGCCGGACGACACCCCGCACGCGGAAAAAGTGCGCCGGCTGGAAGCGCTGAACGAGGTGATCGAGGCCAAGGGCTATG
CGATCAACCAGTCCATGGTCGGCACCGTGCAGCGCGTGCTGGTGGAAAACGTGTCGAAGAAAGACGCCACCATGCTGGCC
GCCCGCACCGCCAACAACCGCGTGGTCAACTTCGCCGGCCATCCGCGCCTCTTGGGCCGGATGATAGAGGTGAAGATCAC
CGCCGCCTTCCCGCATTCGCTGGCTGGCGAGGCGCTGACCAGCGAAAGCGCCTGA

Upstream 100 bases:

>100_bases
CGCATCTGGGTGCTGGGCGACGACGAGATCGAGGCGCAGAAACAGCGCCAGGCCCAGATCCAACCCTGAAACCGAATACC
AGCTGGAAAACCGAGTAGCA

Downstream 100 bases:

>100_bases
GCGTGACGGCGGCGGACGCGCTATCGCCCCGGCCCCGGCCGGCGTATGCTGTGTTCCGTCGCCACACGGAGAATCGCCGA
TGTTCATCGTCTCGCTGACT

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 444; Mature: 444

Protein sequence:

>444_residues
MKKVYIKTFGCQMNEYDSDKMADVLGSAEGMVKTDNPEEADVILFNTCSVREKAQEKVFSDLGRIRPLKEANPDLIIGVG
GCVASQEGDAIVKRAPFVDVVFGPQTLHRLPDLIESRKQSGRSQVDISFPEIEKFDHIPPAKVDGGAAFVSIMEGCSKYC
SFCVVPYTRGEEVSRPFEDVLTEIAGLAAQGVKEITLLGQNVNAYRGLMSDGEIADFALLLEYVHEVPGVERIRFTTSHP
REFSQRIIDCYAKLPKLVSHLHLPVQSGSDRVLMAMKRGYTGLEYKSIIRKLRAIRPDLCLSSDFIIGFPGETEADFEQT
LKLVRDCEFDFSFVFIYSPRPGTPAANLPDDTPHAEKVRRLEALNEVIEAKGYAINQSMVGTVQRVLVENVSKKDATMLA
ARTANNRVVNFAGHPRLLGRMIEVKITAAFPHSLAGEALTSESA

Sequences:

>Translated_444_residues
MKKVYIKTFGCQMNEYDSDKMADVLGSAEGMVKTDNPEEADVILFNTCSVREKAQEKVFSDLGRIRPLKEANPDLIIGVG
GCVASQEGDAIVKRAPFVDVVFGPQTLHRLPDLIESRKQSGRSQVDISFPEIEKFDHIPPAKVDGGAAFVSIMEGCSKYC
SFCVVPYTRGEEVSRPFEDVLTEIAGLAAQGVKEITLLGQNVNAYRGLMSDGEIADFALLLEYVHEVPGVERIRFTTSHP
REFSQRIIDCYAKLPKLVSHLHLPVQSGSDRVLMAMKRGYTGLEYKSIIRKLRAIRPDLCLSSDFIIGFPGETEADFEQT
LKLVRDCEFDFSFVFIYSPRPGTPAANLPDDTPHAEKVRRLEALNEVIEAKGYAINQSMVGTVQRVLVENVSKKDATMLA
ARTANNRVVNFAGHPRLLGRMIEVKITAAFPHSLAGEALTSESA
>Mature_444_residues
MKKVYIKTFGCQMNEYDSDKMADVLGSAEGMVKTDNPEEADVILFNTCSVREKAQEKVFSDLGRIRPLKEANPDLIIGVG
GCVASQEGDAIVKRAPFVDVVFGPQTLHRLPDLIESRKQSGRSQVDISFPEIEKFDHIPPAKVDGGAAFVSIMEGCSKYC
SFCVVPYTRGEEVSRPFEDVLTEIAGLAAQGVKEITLLGQNVNAYRGLMSDGEIADFALLLEYVHEVPGVERIRFTTSHP
REFSQRIIDCYAKLPKLVSHLHLPVQSGSDRVLMAMKRGYTGLEYKSIIRKLRAIRPDLCLSSDFIIGFPGETEADFEQT
LKLVRDCEFDFSFVFIYSPRPGTPAANLPDDTPHAEKVRRLEALNEVIEAKGYAINQSMVGTVQRVLVENVSKKDATMLA
ARTANNRVVNFAGHPRLLGRMIEVKITAAFPHSLAGEALTSESA

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=478, Percent_Identity=38.2845188284519, Blast_Score=311, Evalue=5e-85,
Organism=Homo sapiens, GI28872782, Length=478, Percent_Identity=38.2845188284519, Blast_Score=311, Evalue=9e-85,
Organism=Homo sapiens, GI93277076, Length=459, Percent_Identity=28.322440087146, Blast_Score=177, Evalue=2e-44,
Organism=Escherichia coli, GI1786882, Length=441, Percent_Identity=61.4512471655329, Blast_Score=568, Evalue=1e-163,
Organism=Escherichia coli, GI1787057, Length=445, Percent_Identity=27.4157303370787, Blast_Score=147, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17553146, Length=465, Percent_Identity=35.9139784946237, Blast_Score=281, Evalue=4e-76,
Organism=Caenorhabditis elegans, GI71996771, Length=301, Percent_Identity=30.2325581395349, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI21356207, Length=437, Percent_Identity=34.324942791762, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI19922432, Length=465, Percent_Identity=29.8924731182796, Blast_Score=160, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_CHRVO (Q7NQI8)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903819.1
- ProteinModelPortal:   Q7NQI8
- SMR:   Q7NQI8
- GeneID:   2549789
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_4149
- NMPDR:   fig|243365.1.peg.4149
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q7NQI8
- ProtClustDB:   PRK14325
- BioCyc:   CVIO243365:CV_4149-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49066; Mature: 49066

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVYIKTFGCQMNEYDSDKMADVLGSAEGMVKTDNPEEADVILFNTCSVREKAQEKVFS
CCEEEEEEECCCCCCCCCHHHHHHHCCCCCCEECCCCCCCCEEEEECCCHHHHHHHHHHH
DLGRIRPLKEANPDLIIGVGGCVASQEGDAIVKRAPFVDVVFGPQTLHRLPDLIESRKQS
HHHHCCCCCCCCCCEEEECCCEECCCCCCCCEECCCEEEEEECHHHHHHHHHHHHHHHHC
GRSQVDISFPEIEKFDHIPPAKVDGGAAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDV
CCCEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHHH
LTEIAGLAAQGVKEITLLGQNVNAYRGLMSDGEIADFALLLEYVHEVPGVERIRFTTSHP
HHHHHHHHHHCHHEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCH
REFSQRIIDCYAKLPKLVSHLHLPVQSGSDRVLMAMKRGYTGLEYKSIIRKLRAIRPDLC
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHCCCCCCHHHHHHHHHHHCCCCCC
LSSDFIIGFPGETEADFEQTLKLVRDCEFDFSFVFIYSPRPGTPAANLPDDTPHAEKVRR
CCCCEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHH
LEALNEVIEAKGYAINQSMVGTVQRVLVENVSKKDATMLAARTANNRVVNFAGHPRLLGR
HHHHHHHHHHCCCEECHHHHHHHHHHHHHHCCCHHHHEEEEECCCCCEEEECCCHHHHCE
MIEVKITAAFPHSLAGEALTSESA
EEEEEEEEECCCHHCCCCCCCCCC
>Mature Secondary Structure
MKKVYIKTFGCQMNEYDSDKMADVLGSAEGMVKTDNPEEADVILFNTCSVREKAQEKVFS
CCEEEEEEECCCCCCCCCHHHHHHHCCCCCCEECCCCCCCCEEEEECCCHHHHHHHHHHH
DLGRIRPLKEANPDLIIGVGGCVASQEGDAIVKRAPFVDVVFGPQTLHRLPDLIESRKQS
HHHHCCCCCCCCCCEEEECCCEECCCCCCCCEECCCEEEEEECHHHHHHHHHHHHHHHHC
GRSQVDISFPEIEKFDHIPPAKVDGGAAFVSIMEGCSKYCSFCVVPYTRGEEVSRPFEDV
CCCEEEEECCCHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHHH
LTEIAGLAAQGVKEITLLGQNVNAYRGLMSDGEIADFALLLEYVHEVPGVERIRFTTSHP
HHHHHHHHHHCHHEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCH
REFSQRIIDCYAKLPKLVSHLHLPVQSGSDRVLMAMKRGYTGLEYKSIIRKLRAIRPDLC
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEHHHHCCCCCCHHHHHHHHHHHCCCCCC
LSSDFIIGFPGETEADFEQTLKLVRDCEFDFSFVFIYSPRPGTPAANLPDDTPHAEKVRR
CCCCEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHH
LEALNEVIEAKGYAINQSMVGTVQRVLVENVSKKDATMLAARTANNRVVNFAGHPRLLGR
HHHHHHHHHHCCCEECHHHHHHHHHHHHHHCCCHHHHEEEEECCCCCEEEECCCHHHHCE
MIEVKITAAFPHSLAGEALTSESA
EEEEEEEEECCCHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782