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The map label for this gene is potD [H]

Identifier: 34499554

GI number: 34499554

Start: 4440563

End: 4441627

Strand: Reverse

Name: potD [H]

Synonym: CV_4099

Alternate gene names: 34499554

Gene position: 4441627-4440563 (Counterclockwise)

Preceding gene: 34499555

Following gene: 34499550

Centisome position: 93.49

GC content: 60.56

Gene sequence:

>1065_bases
ATGAAGAAGATCCTCGCGATGCTGGCGCTGGCGGCCTCCACCCAGGCGTTCGCCGCCGAAGAGCTGCATCTGTACAACTG
GAACAACTACCTGTCCGAGAGCGCGGCCAAGAATTTCGAGGCCTATTGCAAGTGCAAGCTGGTGCAGGATTACTACGGTG
ACAACGAAGAACTGCTGGCCAAGCTGGCCGCCGGCGCCAAGGGCTACGACATCGTGGTGCCGACCGGCTTCGCCGTCGAC
GCGCTGATCAAGCAGGGCAAGGCCCAGCCCCTGGACAAGGCGCAGTTGCCCAACTTCAAGAACCTGAACCCGGGTTACCT
GAACAGCTTCTTCGACAAGGGCAACAAGTACTCCGCCCCGTACGCGTTCACCACCACCCTGATCGGCTACAACGAAACCA
AGCTGAAGCAGCTGGGCCTGGCCGACAAGGTGAATAGCTGGGCGCTGATCTTCGATCCGGCGCTGTTGGCCAAGATCAAG
GGCAAGGTCACGGTGCTGGATTCGCAGCGCGAGCTGATCGCCGCCGCGCTGATGTATCTGGGCAAGCCGGCCAATTCCAC
TAACCCGGCCGATTGGAAAGCCGCCCGCGACGTGATCCTGAAGGCCAAGCCGTACTGGGCAGCCTTCAACAACCAGTCCT
ACATCAAGGAACTGACCGTCGGCAACATCTATGTCGCCTTCGGCTACTCCAACGACATGTTCCAGGCCCAGCAGGACGCC
AAGAAGGCCAAGCGCGCTTTCGCGCTGAACTTCAGCCTGCAGAAGGAAGGCAATACCCTGTCGCTGGACAACTTCGTGGT
GCTGAAGGACGCGCCGCGCAAGGATCTGGCCTACAAGTTCATCAACTTCATGCTGGACGGCAAGAACGCCGCCGGCCTGA
CCAACGAGATGGGCAACGGCAATCCGAACGCCGCCGCCGGCAAGTTCGTCAAGACCGAGCTGACCAAGATCCCGGCGATC
TTCCCGACCAGCACCGACCTGCCGCGCCTGCAGCAGCTGCACGATCTGAACGCCAAGGATCGCCGCGAGCTGAACAAGGT
GTGGTCCGAGATCAAGCTGAAGTAA

Upstream 100 bases:

>100_bases
GCATAATGCCTTGCCGTCGCCGCCGCACCCGGGCGACGCAAGGGGGTGTCTCCCGGTTTTCTCGTAGTATCACCAAGCTG
TGCAATACAAAGGAAACGAT

Downstream 100 bases:

>100_bases
GAGGGTCCGCGCGATTCAGCAAAAAGGCCGGGAAGTTTCCCGGCCTTTTTCTATATATGTGTGCTGGGTCAGTCCAGGTG
TAGCCGCAGCAACTGGCTGA

Product: polyamine ABC transporter protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MKKILAMLALAASTQAFAAEELHLYNWNNYLSESAAKNFEAYCKCKLVQDYYGDNEELLAKLAAGAKGYDIVVPTGFAVD
ALIKQGKAQPLDKAQLPNFKNLNPGYLNSFFDKGNKYSAPYAFTTTLIGYNETKLKQLGLADKVNSWALIFDPALLAKIK
GKVTVLDSQRELIAAALMYLGKPANSTNPADWKAARDVILKAKPYWAAFNNQSYIKELTVGNIYVAFGYSNDMFQAQQDA
KKAKRAFALNFSLQKEGNTLSLDNFVVLKDAPRKDLAYKFINFMLDGKNAAGLTNEMGNGNPNAAAGKFVKTELTKIPAI
FPTSTDLPRLQQLHDLNAKDRRELNKVWSEIKLK

Sequences:

>Translated_354_residues
MKKILAMLALAASTQAFAAEELHLYNWNNYLSESAAKNFEAYCKCKLVQDYYGDNEELLAKLAAGAKGYDIVVPTGFAVD
ALIKQGKAQPLDKAQLPNFKNLNPGYLNSFFDKGNKYSAPYAFTTTLIGYNETKLKQLGLADKVNSWALIFDPALLAKIK
GKVTVLDSQRELIAAALMYLGKPANSTNPADWKAARDVILKAKPYWAAFNNQSYIKELTVGNIYVAFGYSNDMFQAQQDA
KKAKRAFALNFSLQKEGNTLSLDNFVVLKDAPRKDLAYKFINFMLDGKNAAGLTNEMGNGNPNAAAGKFVKTELTKIPAI
FPTSTDLPRLQQLHDLNAKDRRELNKVWSEIKLK
>Mature_354_residues
MKKILAMLALAASTQAFAAEELHLYNWNNYLSESAAKNFEAYCKCKLVQDYYGDNEELLAKLAAGAKGYDIVVPTGFAVD
ALIKQGKAQPLDKAQLPNFKNLNPGYLNSFFDKGNKYSAPYAFTTTLIGYNETKLKQLGLADKVNSWALIFDPALLAKIK
GKVTVLDSQRELIAAALMYLGKPANSTNPADWKAARDVILKAKPYWAAFNNQSYIKELTVGNIYVAFGYSNDMFQAQQDA
KKAKRAFALNFSLQKEGNTLSLDNFVVLKDAPRKDLAYKFINFMLDGKNAAGLTNEMGNGNPNAAAGKFVKTELTKIPAI
FPTSTDLPRLQQLHDLNAKDRRELNKVWSEIKLK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=352, Percent_Identity=37.5, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI1787367, Length=318, Percent_Identity=30.5031446540881, Blast_Score=144, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39281; Mature: 39281

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKILAMLALAASTQAFAAEELHLYNWNNYLSESAAKNFEAYCKCKLVQDYYGDNEELLA
CHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCHHHHEEEEEHHHHCCCHHHHH
KLAAGAKGYDIVVPTGFAVDALIKQGKAQPLDKAQLPNFKNLNPGYLNSFFDKGNKYSAP
HHHCCCCCCEEEEECCHHHHHHHHCCCCCCCCHHCCCCCCCCCCHHHHHHHHCCCCCCCC
YAFTTTLIGYNETKLKQLGLADKVNSWALIFDPALLAKIKGKVTVLDSQRELIAAALMYL
EEEEEEEECCCHHHHHHHCCHHCCCCEEEEECHHHHHHHCCCEEEECCHHHHHHHHHHHH
GKPANSTNPADWKAARDVILKAKPYWAAFNNQSYIKELTVGNIYVAFGYSNDMFQAQQDA
CCCCCCCCCCHHHHHHHHEEECCCCEEECCCCHHHHHEEECEEEEEECCCCCHHHHHHHH
KKAKRAFALNFSLQKEGNTLSLDNFVVLKDAPRKDLAYKFINFMLDGKNAAGLTNEMGNG
HHHHHEEEEEEEEECCCCEEEECCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
NPNAAAGKFVKTELTKIPAIFPTSTDLPRLQQLHDLNAKDRRELNKVWSEIKLK
CCCCHHCHHHHHHHHHCCEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKILAMLALAASTQAFAAEELHLYNWNNYLSESAAKNFEAYCKCKLVQDYYGDNEELLA
CHHHHHHHHHHHHHHHHHHHHEEEECCHHHHHHHHHHCCHHHHEEEEEHHHHCCCHHHHH
KLAAGAKGYDIVVPTGFAVDALIKQGKAQPLDKAQLPNFKNLNPGYLNSFFDKGNKYSAP
HHHCCCCCCEEEEECCHHHHHHHHCCCCCCCCHHCCCCCCCCCCHHHHHHHHCCCCCCCC
YAFTTTLIGYNETKLKQLGLADKVNSWALIFDPALLAKIKGKVTVLDSQRELIAAALMYL
EEEEEEEECCCHHHHHHHCCHHCCCCEEEEECHHHHHHHCCCEEEECCHHHHHHHHHHHH
GKPANSTNPADWKAARDVILKAKPYWAAFNNQSYIKELTVGNIYVAFGYSNDMFQAQQDA
CCCCCCCCCCHHHHHHHHEEECCCCEEECCCCHHHHHEEECEEEEEECCCCCHHHHHHHH
KKAKRAFALNFSLQKEGNTLSLDNFVVLKDAPRKDLAYKFINFMLDGKNAAGLTNEMGNG
HHHHHEEEEEEEEECCCCEEEECCEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
NPNAAAGKFVKTELTKIPAIFPTSTDLPRLQQLHDLNAKDRRELNKVWSEIKLK
CCCCHHCHHHHHHHHHCCEECCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]