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The map label for this gene is pleD [H]

Identifier: 34499542

GI number: 34499542

Start: 4423775

End: 4424524

Strand: Direct

Name: pleD [H]

Synonym: CV_4087

Alternate gene names: 34499542

Gene position: 4423775-4424524 (Clockwise)

Preceding gene: 34499541

Following gene: 34499544

Centisome position: 93.11

GC content: 67.07

Gene sequence:

>750_bases
ATGACTCCCTGGCCGTTCAGCGCCCGCCGCATACGCAAGGAGCTGATCCTGATCCTGCTGCTCAACCTGGGCTGCTATCT
GCTGTTCGTGGATTGGGATCTGTTCGAGAAGCTGGATGTCTGGCTGCACCAGCACCCGACATCCCAGCTGGACGAAGTGC
TGCTGCTCAGCTTCACAATGGCGCTATCGATGACCTGGTTCGCCGTCCGCCGCCTGCGCGAGGCGCGCCAGTTGCTGAAG
GCGCTGCGCCGCCAGGCCGAGCGCGACGACATCACCGGCCTGGCCAACCGCCGTCGCGCCAACCAGGCGCTGCAGCGCGA
GACCGAACGCAGCTACCGCTCGGGACGACCTTACTCGGTGCTGCTGCTCGATCTCGACCACTTCAAGCACATCAACGACA
GCCACGGCCATCTTGCCGGCGACATGGTGCTGCAGCGCTTCGGCCAGGCGCTGCGCCAAAGGGCGCGCCAGCTCGACACC
GTCGCCCGCTGGGGCGGCGAGGAGTTCATCATCATCTGTCCGGAAACCGAACAGGCCGGCGCGCTGACGCTGGCCGAAAA
CATCGGCCAGCTGCTGCGCGAGACCGATTTCGGCGCAGTCGCCCGCGTCACCGCCAGCATAGGCGTGGCGACGCTGTCCG
ACGACGACACGCCGGACAGCCTGGTCCATCGCGCCGACATGCACTTGTACGCCGCCAAGCAGGCGGGGCGGGACCGGGTG
GTCGGAGAAGGCTGCGCCGTCCGGCCATGA

Upstream 100 bases:

>100_bases
GACGCCGCTGGCGGGGATGATGGCCGTCTGAAACGGCCCGCTTGCCCCTGAGGACGCGATGGACTAGCAAGAAAATAGTC
CGCCTCCCCTCAAGGGCATC

Downstream 100 bases:

>100_bases
AAAAACCGGCGCCAAGGCGCCGGTTCTTATCGGAGGGAGATGCCGGTCAGAGCACGCCCACGCTCTGTTGCAGCGCGCTG
CGCTTGGCAGACAGGTCCAG

Product: hypothetical protein

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 249; Mature: 248

Protein sequence:

>249_residues
MTPWPFSARRIRKELILILLLNLGCYLLFVDWDLFEKLDVWLHQHPTSQLDEVLLLSFTMALSMTWFAVRRLREARQLLK
ALRRQAERDDITGLANRRRANQALQRETERSYRSGRPYSVLLLDLDHFKHINDSHGHLAGDMVLQRFGQALRQRARQLDT
VARWGGEEFIIICPETEQAGALTLAENIGQLLRETDFGAVARVTASIGVATLSDDDTPDSLVHRADMHLYAAKQAGRDRV
VGEGCAVRP

Sequences:

>Translated_249_residues
MTPWPFSARRIRKELILILLLNLGCYLLFVDWDLFEKLDVWLHQHPTSQLDEVLLLSFTMALSMTWFAVRRLREARQLLK
ALRRQAERDDITGLANRRRANQALQRETERSYRSGRPYSVLLLDLDHFKHINDSHGHLAGDMVLQRFGQALRQRARQLDT
VARWGGEEFIIICPETEQAGALTLAENIGQLLRETDFGAVARVTASIGVATLSDDDTPDSLVHRADMHLYAAKQAGRDRV
VGEGCAVRP
>Mature_248_residues
TPWPFSARRIRKELILILLLNLGCYLLFVDWDLFEKLDVWLHQHPTSQLDEVLLLSFTMALSMTWFAVRRLREARQLLKA
LRRQAERDDITGLANRRRANQALQRETERSYRSGRPYSVLLLDLDHFKHINDSHGHLAGDMVLQRFGQALRQRARQLDTV
ARWGGEEFIIICPETEQAGALTLAENIGQLLRETDFGAVARVTASIGVATLSDDDTPDSLVHRADMHLYAAKQAGRDRVV
GEGCAVRP

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI87081881, Length=174, Percent_Identity=39.6551724137931, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI145693134, Length=170, Percent_Identity=37.0588235294118, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI87082007, Length=198, Percent_Identity=36.3636363636364, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787262, Length=196, Percent_Identity=31.6326530612245, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1788381, Length=179, Percent_Identity=32.9608938547486, Blast_Score=94, Evalue=8e-21,
Organism=Escherichia coli, GI1786584, Length=161, Percent_Identity=34.1614906832298, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787816, Length=192, Percent_Identity=33.3333333333333, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI1787802, Length=193, Percent_Identity=33.160621761658, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787541, Length=182, Percent_Identity=32.4175824175824, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081977, Length=170, Percent_Identity=33.5294117647059, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI87081974, Length=162, Percent_Identity=29.6296296296296, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1788085, Length=171, Percent_Identity=30.4093567251462, Blast_Score=62, Evalue=5e-11,
Organism=Escherichia coli, GI1788956, Length=164, Percent_Identity=29.8780487804878, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 28410; Mature: 28279

Theoretical pI: Translated: 8.12; Mature: 8.12

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPWPFSARRIRKELILILLLNLGCYLLFVDWDLFEKLDVWLHQHPTSQLDEVLLLSFTM
CCCCCCHHHHHHHHHHHHHHHHHHHEEEHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ALSMTWFAVRRLREARQLLKALRRQAERDDITGLANRRRANQALQRETERSYRSGRPYSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LLLDLDHFKHINDSHGHLAGDMVLQRFGQALRQRARQLDTVARWGGEEFIIICPETEQAG
EEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
ALTLAENIGQLLRETDFGAVARVTASIGVATLSDDDTPDSLVHRADMHLYAAKQAGRDRV
HHHHHHHHHHHHHHCCCHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCC
VGEGCAVRP
CCCCCCCCC
>Mature Secondary Structure 
TPWPFSARRIRKELILILLLNLGCYLLFVDWDLFEKLDVWLHQHPTSQLDEVLLLSFTM
CCCCCHHHHHHHHHHHHHHHHHHHEEEHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ALSMTWFAVRRLREARQLLKALRRQAERDDITGLANRRRANQALQRETERSYRSGRPYSV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
LLLDLDHFKHINDSHGHLAGDMVLQRFGQALRQRARQLDTVARWGGEEFIIICPETEQAG
EEEEHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCC
ALTLAENIGQLLRETDFGAVARVTASIGVATLSDDDTPDSLVHRADMHLYAAKQAGRDRV
HHHHHHHHHHHHHHCCCHHHHHHHHHHCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCC
VGEGCAVRP
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA