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The map label for this gene is fliY [H]

Identifier: 34499508

GI number: 34499508

Start: 4383957

End: 4384742

Strand: Reverse

Name: fliY [H]

Synonym: CV_4053

Alternate gene names: 34499508

Gene position: 4384742-4383957 (Counterclockwise)

Preceding gene: 34499509

Following gene: 34499507

Centisome position: 92.29

GC content: 62.6

Gene sequence:

>786_bases
ATGAAGAAATGGAAGGCCGTACTATTGTTGACAGCCGTGGGCAGCCATGCCTTCGCGGCGGACCTGTTGGACACGGTCAA
ACAGCGTGGCGCGCTGAAGATCGCGCTGGAGGGTACCTACCCGCCGTTCAACTTCAAGGACGCGAAGCAGCAGTTGACCG
GTTTCGACGTCGAGATCGCCACCGAGATCGCGCGCCGGATGAAAGTGAAACCTGAATTCGTTACCGCCGAGTGGAGCGGG
CTGCTGGCCGGCCTGCAGAGCGGCAAATTCGACATCGTGGTCAACCAGGTCGGCGTGACCGCGCAGCGCCAGGCGGTGTT
CGATTTCAGCCAGCCGTACACCTACTCCAGCGCCCAGCTGATCGTGCGCAAGAATGAAAGTCGCGACTTCAAGAACCTGG
CCGACCTGAAGGGCAAAAAGCTCGGCGTCGGCCAGGGCAGCAACTACGCCGACATGGCCAAGGCGGCTGCCGGCGTGCAG
GTGAAGACTTACCCCGGCGCGCCGGAATACCTGCAGGATCTGGCCACCGGCCGGCTGGACGCGGCGCTGAACGACAGCCT
GCTGATTCCGTTCGCGATCAAGCAGGCCAAGCTGCCGCTGAAGGCCGGCGCCCCGGTGGGCGAGGTGGCGGCGATGGCGA
TTCCGTTCGCCAAGGGCAATCCGCAATTCAAATCCGAAATCGACAAGGCCTTGGGCGGCATGAAGGCCGACGGCACCTTC
CGGAAAATTTCCGTGAAGTGGTTCGGCATCGACGTGTCCAAGGCGCCTGCCGCGGCGAAGAAATAA

Upstream 100 bases:

>100_bases
GCGAATCAATCAGGTTTAATCGGCTGGTCTGCATCAATATTTACTGATATTCTTTTGTTAAATTATTTTTATCATTTATA
TTGCAAAAGGTTATATTGAT

Downstream 100 bases:

>100_bases
GCCGCTTTGTGATAGCATCGACCGCGCGCCCCTGCATTAGCGGGGGCGTCGGTTTTTAAGGAAGGGAAGAGATGGGCGAG
TGGATGGCTCTGTTCGAGGC

Product: amino acid ABC transporter substrate-binding protein

Products: NA

Alternate protein names: CBP; Protein fliY; Sulfate starvation-induced protein 7; SSI7 [H]

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MKKWKAVLLLTAVGSHAFAADLLDTVKQRGALKIALEGTYPPFNFKDAKQQLTGFDVEIATEIARRMKVKPEFVTAEWSG
LLAGLQSGKFDIVVNQVGVTAQRQAVFDFSQPYTYSSAQLIVRKNESRDFKNLADLKGKKLGVGQGSNYADMAKAAAGVQ
VKTYPGAPEYLQDLATGRLDAALNDSLLIPFAIKQAKLPLKAGAPVGEVAAMAIPFAKGNPQFKSEIDKALGGMKADGTF
RKISVKWFGIDVSKAPAAAKK

Sequences:

>Translated_261_residues
MKKWKAVLLLTAVGSHAFAADLLDTVKQRGALKIALEGTYPPFNFKDAKQQLTGFDVEIATEIARRMKVKPEFVTAEWSG
LLAGLQSGKFDIVVNQVGVTAQRQAVFDFSQPYTYSSAQLIVRKNESRDFKNLADLKGKKLGVGQGSNYADMAKAAAGVQ
VKTYPGAPEYLQDLATGRLDAALNDSLLIPFAIKQAKLPLKAGAPVGEVAAMAIPFAKGNPQFKSEIDKALGGMKADGTF
RKISVKWFGIDVSKAPAAAKK
>Mature_261_residues
MKKWKAVLLLTAVGSHAFAADLLDTVKQRGALKIALEGTYPPFNFKDAKQQLTGFDVEIATEIARRMKVKPEFVTAEWSG
LLAGLQSGKFDIVVNQVGVTAQRQAVFDFSQPYTYSSAQLIVRKNESRDFKNLADLKGKKLGVGQGSNYADMAKAAAGVQ
VKTYPGAPEYLQDLATGRLDAALNDSLLIPFAIKQAKLPLKAGAPVGEVAAMAIPFAKGNPQFKSEIDKALGGMKADGTF
RKISVKWFGIDVSKAPAAAKK

Specific function: Part of a binding-protein-dependent transport system for cystine [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788228, Length=251, Percent_Identity=39.4422310756972, Blast_Score=185, Evalue=2e-48,
Organism=Escherichia coli, GI1787031, Length=248, Percent_Identity=30.6451612903226, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1788649, Length=231, Percent_Identity=32.4675324675325, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788648, Length=231, Percent_Identity=28.1385281385281, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1787085, Length=222, Percent_Identity=27.4774774774775, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787088, Length=252, Percent_Identity=25.3968253968254, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: 1920 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1060 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 28051; Mature: 28051

Theoretical pI: Translated: 10.38; Mature: 10.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWKAVLLLTAVGSHAFAADLLDTVKQRGALKIALEGTYPPFNFKDAKQQLTGFDVEIA
CCCHHEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCCHHHH
TEIARRMKVKPEFVTAEWSGLLAGLQSGKFDIVVNQVGVTAQRQAVFDFSQPYTYSSAQL
HHHHHHHCCCCCEEEHHHHHHHHHHCCCCEEEEEECCCCCCHHHEEEECCCCCCCCCEEE
IVRKNESRDFKNLADLKGKKLGVGQGSNYADMAKAAAGVQVKTYPGAPEYLQDLATGRLD
EEEECCCCCHHHHHHCCCCEECCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCC
AALNDSLLIPFAIKQAKLPLKAGAPVGEVAAMAIPFAKGNPQFKSEIDKALGGMKADGTF
HHCCCCEEEEEEHHHCCCCCCCCCCHHHHHHHEEECCCCCCHHHHHHHHHHCCCCCCCCE
RKISVKWFGIDVSKAPAAAKK
EEEEEEEEEEECCCCCCCCCC
>Mature Secondary Structure
MKKWKAVLLLTAVGSHAFAADLLDTVKQRGALKIALEGTYPPFNFKDAKQQLTGFDVEIA
CCCHHEEEEEEECCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHCCCCHHHH
TEIARRMKVKPEFVTAEWSGLLAGLQSGKFDIVVNQVGVTAQRQAVFDFSQPYTYSSAQL
HHHHHHHCCCCCEEEHHHHHHHHHHCCCCEEEEEECCCCCCHHHEEEECCCCCCCCCEEE
IVRKNESRDFKNLADLKGKKLGVGQGSNYADMAKAAAGVQVKTYPGAPEYLQDLATGRLD
EEEECCCCCHHHHHHCCCCEECCCCCCCHHHHHHHHCCCEEEECCCCHHHHHHHHCCCCC
AALNDSLLIPFAIKQAKLPLKAGAPVGEVAAMAIPFAKGNPQFKSEIDKALGGMKADGTF
HHCCCCEEEEEEHHHCCCCCCCCCCHHHHHHHEEECCCCCCHHHHHHHHHHCCCCCCCCE
RKISVKWFGIDVSKAPAAAKK
EEEEEEEEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]