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The map label for this gene is rfe [H]

Identifier: 34499471

GI number: 34499471

Start: 4342106

End: 4343209

Strand: Reverse

Name: rfe [H]

Synonym: CV_4016

Alternate gene names: 34499471

Gene position: 4343209-4342106 (Counterclockwise)

Preceding gene: 34499473

Following gene: 34499470

Centisome position: 91.42

GC content: 51.0

Gene sequence:

>1104_bases
ATGTTACAGATTCTATTGTTGGCTACATTTTTATCTTTACTCGTCGGTATGTGCCTGATCCACTTCGCACATTTGCACGA
GAGCTTTTCGGCAGACCATGATCTTGGGGGGGTGCAGAAGTTCCACGCTCGCCCGGTGCCGCGTATCGGTGGGGTACCGA
TTGCTGCCGGTTTGGTTGCTGGGTGCGGTTTGTTGGCTTGGCTACAGAAGGATGTTTTGTCGCTGTTGCTATTGGTGGTG
GCGTTGCCGGCTTTTGCTGCGGGGATGCTTGAGGATGTGACAAAGAAAGTGGGGCCGTTGCCAAGGCTGCTGGCTACTTT
CGTTGCAGCAATTTTGGGCTATTTCATGCTGGGGGCTGGTGTGTGGCATTTGGACCTTCCTTTCGTGGACGCCTTGTTGT
CACGGTTCTGGATGCTGTCCCTTGTGTTGACTATGGTAGCGGTTGGTGGTGTGGCGCATGCGGTCAACATCATCGATGGC
TACAATGGCTTGTCTGGTGTAGTGTCTATTTTCATTCTTCTGGCAATCGCGTATGTAGCGTTCAAGATGCAGGATGTTGC
GTTGATGGGTGTTTGTTTTGCCATGGTGGGGGCGATTGCGGGCTTCTTGTTTTGGAATTTCCCGCGAGGATTGATCTTTG
CGGGCGATGGTGGTGCCTACTTGGTAGGCTTCATGATCGCCGAAATGTCAGTGTTGCTGGTGGCACGTCATCCGCAGGTA
TCGCCATGGTTTCCGCTGCTATGCGTGATATATCCGGTGTTTGAGACCTTGTTCTCGATTTATCGTCGTAAGTTCCTGCA
AGGGCGCCGGGTCGGTTATCCTGATGCTCTGCACCTACATCAGTTAATTTATATGCGCCTGGTACGCTGGATGGTAGGGA
GTAAGGATGTTGATCACATGACCATGCGTAATAGCCTAACCTCTCCCTACCTCTGGGCGCTATCTTCTGTGTCCGTAGTG
CCTGCTATGTTGTTTTGGGACAATACAGTAGTATTGATGTGTTTCGTCCTTATTTTCATGATTACCTATGTGTACTTGTA
CCGGATGATCATCCGATTCCGTACGCCGCGTTGGATGTTGCTGCGCAAGTCTATGCGTAACTGA

Upstream 100 bases:

>100_bases
TGGCTTGAAAATTGCATGAGGTTTACAATGACAAGTCAAGTTTTGTTGCCGTCGGACGGTACTAAAATATCGGCATCATC
AAATATTGAAGAGAAGCTTA

Downstream 100 bases:

>100_bases
TCTGATCTTCACATGAGAGCCTGCAGAAAATTGCTGGTACAGGCTCTTGAAAATCACCACTCGCATCCCTATCATTAGCA
CTCGCAATGGTCGAGTGCTA

Product: undecaprenyl-phosphate alpha-N-acetylglucosaminyltransferase

Products: UMP; Undecaprenyl N-Acetyl-Alpha-D-Glucosaminyl Pyrophosphate. [C]

Alternate protein names: UDP-GlcNAc:undecaprenyl-phosphate GlcNAc-1-phosphate transferase; Undecaprenyl-Phosphate GlcNAc-1-phosphate transferase [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MLQILLLATFLSLLVGMCLIHFAHLHESFSADHDLGGVQKFHARPVPRIGGVPIAAGLVAGCGLLAWLQKDVLSLLLLVV
ALPAFAAGMLEDVTKKVGPLPRLLATFVAAILGYFMLGAGVWHLDLPFVDALLSRFWMLSLVLTMVAVGGVAHAVNIIDG
YNGLSGVVSIFILLAIAYVAFKMQDVALMGVCFAMVGAIAGFLFWNFPRGLIFAGDGGAYLVGFMIAEMSVLLVARHPQV
SPWFPLLCVIYPVFETLFSIYRRKFLQGRRVGYPDALHLHQLIYMRLVRWMVGSKDVDHMTMRNSLTSPYLWALSSVSVV
PAMLFWDNTVVLMCFVLIFMITYVYLYRMIIRFRTPRWMLLRKSMRN

Sequences:

>Translated_367_residues
MLQILLLATFLSLLVGMCLIHFAHLHESFSADHDLGGVQKFHARPVPRIGGVPIAAGLVAGCGLLAWLQKDVLSLLLLVV
ALPAFAAGMLEDVTKKVGPLPRLLATFVAAILGYFMLGAGVWHLDLPFVDALLSRFWMLSLVLTMVAVGGVAHAVNIIDG
YNGLSGVVSIFILLAIAYVAFKMQDVALMGVCFAMVGAIAGFLFWNFPRGLIFAGDGGAYLVGFMIAEMSVLLVARHPQV
SPWFPLLCVIYPVFETLFSIYRRKFLQGRRVGYPDALHLHQLIYMRLVRWMVGSKDVDHMTMRNSLTSPYLWALSSVSVV
PAMLFWDNTVVLMCFVLIFMITYVYLYRMIIRFRTPRWMLLRKSMRN
>Mature_367_residues
MLQILLLATFLSLLVGMCLIHFAHLHESFSADHDLGGVQKFHARPVPRIGGVPIAAGLVAGCGLLAWLQKDVLSLLLLVV
ALPAFAAGMLEDVTKKVGPLPRLLATFVAAILGYFMLGAGVWHLDLPFVDALLSRFWMLSLVLTMVAVGGVAHAVNIIDG
YNGLSGVVSIFILLAIAYVAFKMQDVALMGVCFAMVGAIAGFLFWNFPRGLIFAGDGGAYLVGFMIAEMSVLLVARHPQV
SPWFPLLCVIYPVFETLFSIYRRKFLQGRRVGYPDALHLHQLIYMRLVRWMVGSKDVDHMTMRNSLTSPYLWALSSVSVV
PAMLFWDNTVVLMCFVLIFMITYVYLYRMIIRFRTPRWMLLRKSMRN

Specific function: Catalyzes the formation of undecaprenyl-PP-N- acetylglucosamine. Involved in the synthesis of anionic cell-wall polymers as it mediates the initiation of the linkage unit formation that appears to be common to the two types of teichoic acids attached to t

COG id: COG0472

COG function: function code M; UDP-N-acetylmuramyl pentapeptide phosphotransferase/UDP-N-acetylglucosamine-1-phosphate transferase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1790218, Length=264, Percent_Identity=26.1363636363636, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000715
- InterPro:   IPR018480 [H]

Pfam domain/function: PF00953 Glycos_transf_4 [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 40906; Mature: 40906

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
7.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
5.7 %Met     (Mature Protein)
7.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQILLLATFLSLLVGMCLIHFAHLHESFSADHDLGGVQKFHARPVPRIGGVPIAAGLVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHH
GCGLLAWLQKDVLSLLLLVVALPAFAAGMLEDVTKKVGPLPRLLATFVAAILGYFMLGAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
VWHLDLPFVDALLSRFWMLSLVLTMVAVGGVAHAVNIIDGYNGLSGVVSIFILLAIAYVA
HHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FKMQDVALMGVCFAMVGAIAGFLFWNFPRGLIFAGDGGAYLVGFMIAEMSVLLVARHPQV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCC
SPWFPLLCVIYPVFETLFSIYRRKFLQGRRVGYPDALHLHQLIYMRLVRWMVGSKDVDHM
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
TMRNSLTSPYLWALSSVSVVPAMLFWDNTVVLMCFVLIFMITYVYLYRMIIRFRTPRWML
HHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LRKSMRN
HHHHHCC
>Mature Secondary Structure
MLQILLLATFLSLLVGMCLIHFAHLHESFSADHDLGGVQKFHARPVPRIGGVPIAAGLVA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHH
GCGLLAWLQKDVLSLLLLVVALPAFAAGMLEDVTKKVGPLPRLLATFVAAILGYFMLGAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCC
VWHLDLPFVDALLSRFWMLSLVLTMVAVGGVAHAVNIIDGYNGLSGVVSIFILLAIAYVA
HHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
FKMQDVALMGVCFAMVGAIAGFLFWNFPRGLIFAGDGGAYLVGFMIAEMSVLLVARHPQV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHCCCCC
SPWFPLLCVIYPVFETLFSIYRRKFLQGRRVGYPDALHLHQLIYMRLVRWMVGSKDVDHM
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHH
TMRNSLTSPYLWALSSVSVVPAMLFWDNTVVLMCFVLIFMITYVYLYRMIIRFRTPRWML
HHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
LRKSMRN
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: UDP-N-Acetyl-D-Glucosamine; Undecaprenyl Phosphate [C]

Specific reaction: UDP-N-Acetyl-D-Glucosamine + Undecaprenyl Phosphate = UMP + Undecaprenyl N-Acetyl-Alpha-D-Glucosaminyl Pyrophosphate. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12101296; 9384377 [H]