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The map label for this gene is sspA [H]

Identifier: 34499460

GI number: 34499460

Start: 4331740

End: 4332342

Strand: Reverse

Name: sspA [H]

Synonym: CV_4005

Alternate gene names: 34499460

Gene position: 4332342-4331740 (Counterclockwise)

Preceding gene: 34499461

Following gene: 34499459

Centisome position: 91.19

GC content: 59.37

Gene sequence:

>603_bases
ATGATGACCCTCTACTCCGGAATCACCTGCCCGTTCAGCCAGCGCTGCCGTATCGTGCTCTTCGAAAAGGGAATGGATTT
CGAAATCATCGATGTGGATATCCATAACAAGCCGGAAGACCTGGCTGTGATGAACCCGTACAACGAAGTGCCGGTTCTGG
TGGAGCGCGACCTGATCCTGCACGAGTCCAACATCATCAACGAATACATCGACGAGCGTTTCCCGCACCCGCAGCTGATG
CCGGCCGATCCGGTGATGCGCGCCCGCGCCCGCCTGTTCCTGTACCGTTTCGAGAACGAGCTGTTCATCCACGTGAAGAC
GCTGGAAGCCGGCGCCACCGGCAAGGAAGCGACCAAGGCGCGCGAAGCCATCCGCGACGGCCTGACCACCATCGCCCCGA
TCTTCTCCAAGCAGAAATTCATGCTGGGCGACGATTTCTCGATGATCGACGTGGCCATCGCGCCGCTGATGTGGCGCCTG
GAGCATTACAACATCGATCTGGGCAAGAGCGCCGCGCCCATCCTGAAGTACGCCGAGCGCATCTTCCAGCGCCAGTCCTT
CATCGATTCGCTGACGCCGTCCGAAAAGGCGATGCGCAAGTAA

Upstream 100 bases:

>100_bases
TAATCATGATGTTGTGCTGAAAAACTGGAAGGGGACGGTTGAGAGTGCTACAATGCGCTGCTGCATCCATTCATTAAGCT
AGTCAAGGATTTTCTCCGCC

Downstream 100 bases:

>100_bases
GGAGACGCGATGAGCTCCAGCACCAAGCCGTACATGCTGCGCGCGCTGCACCAATGGTGCGGCGACAATGGCTACACCCC
GTACATCGTGGTGTGGGTGA

Product: stringent starvation protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 200; Mature: 200

Protein sequence:

>200_residues
MMTLYSGITCPFSQRCRIVLFEKGMDFEIIDVDIHNKPEDLAVMNPYNEVPVLVERDLILHESNIINEYIDERFPHPQLM
PADPVMRARARLFLYRFENELFIHVKTLEAGATGKEATKAREAIRDGLTTIAPIFSKQKFMLGDDFSMIDVAIAPLMWRL
EHYNIDLGKSAAPILKYAERIFQRQSFIDSLTPSEKAMRK

Sequences:

>Translated_200_residues
MMTLYSGITCPFSQRCRIVLFEKGMDFEIIDVDIHNKPEDLAVMNPYNEVPVLVERDLILHESNIINEYIDERFPHPQLM
PADPVMRARARLFLYRFENELFIHVKTLEAGATGKEATKAREAIRDGLTTIAPIFSKQKFMLGDDFSMIDVAIAPLMWRL
EHYNIDLGKSAAPILKYAERIFQRQSFIDSLTPSEKAMRK
>Mature_200_residues
MMTLYSGITCPFSQRCRIVLFEKGMDFEIIDVDIHNKPEDLAVMNPYNEVPVLVERDLILHESNIINEYIDERFPHPQLM
PADPVMRARARLFLYRFENELFIHVKTLEAGATGKEATKAREAIRDGLTTIAPIFSKQKFMLGDDFSMIDVAIAPLMWRL
EHYNIDLGKSAAPILKYAERIFQRQSFIDSLTPSEKAMRK

Specific function: Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI38016131, Length=179, Percent_Identity=32.4022346368715, Blast_Score=97, Evalue=8e-21,
Organism=Homo sapiens, GI4758484, Length=200, Percent_Identity=33, Blast_Score=97, Evalue=1e-20,
Organism=Homo sapiens, GI300360569, Length=170, Percent_Identity=32.9411764705882, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI300360534, Length=194, Percent_Identity=32.9896907216495, Blast_Score=95, Evalue=3e-20,
Organism=Homo sapiens, GI300360532, Length=194, Percent_Identity=31.9587628865979, Blast_Score=86, Evalue=2e-17,
Organism=Homo sapiens, GI300360567, Length=177, Percent_Identity=30.5084745762712, Blast_Score=77, Evalue=7e-15,
Organism=Homo sapiens, GI22202624, Length=173, Percent_Identity=28.3236994219653, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI300360571, Length=168, Percent_Identity=30.952380952381, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI1789624, Length=200, Percent_Identity=46.5, Blast_Score=183, Evalue=7e-48,
Organism=Caenorhabditis elegans, GI17551302, Length=177, Percent_Identity=31.638418079096, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21355779, Length=176, Percent_Identity=30.1136363636364, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI21355775, Length=175, Percent_Identity=30.2857142857143, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24660979, Length=98, Percent_Identity=36.734693877551, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 23125; Mature: 23125

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTLYSGITCPFSQRCRIVLFEKGMDFEIIDVDIHNKPEDLAVMNPYNEVPVLVERDLIL
CCEECCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCEEEECCCCCCCEEEECCCHH
HESNIINEYIDERFPHPQLMPADPVMRARARLFLYRFENELFIHVKTLEAGATGKEATKA
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHEEEEECCEEEEEEEEECCCCCCHHHHHH
REAIRDGLTTIAPIFSKQKFMLGDDFSMIDVAIAPLMWRLEHYNIDLGKSAAPILKYAER
HHHHHHCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
IFQRQSFIDSLTPSEKAMRK
HHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure
MMTLYSGITCPFSQRCRIVLFEKGMDFEIIDVDIHNKPEDLAVMNPYNEVPVLVERDLIL
CCEECCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCCCEEEECCCCCCCEEEECCCHH
HESNIINEYIDERFPHPQLMPADPVMRARARLFLYRFENELFIHVKTLEAGATGKEATKA
HHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHEEEEECCEEEEEEEEECCCCCCHHHHHH
REAIRDGLTTIAPIFSKQKFMLGDDFSMIDVAIAPLMWRLEHYNIDLGKSAAPILKYAER
HHHHHHCHHHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
IFQRQSFIDSLTPSEKAMRK
HHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]