Click here to switch to the map view.

The map label for this gene is dsbA [H]

Identifier: 34499453

GI number: 34499453

Start: 4325946

End: 4326557

Strand: Reverse

Name: dsbA [H]

Synonym: CV_3998

Alternate gene names: 34499453

Gene position: 4326557-4325946 (Counterclockwise)

Preceding gene: 34499454

Following gene: 34499452

Centisome position: 91.06

GC content: 59.31

Gene sequence:

>612_bases
ATGAAGAAATGGTTGCTGTTGGTAATGCTGGCAGTCAGCGGCATGGCGAATGCCGCGATCCAGTTGGGCAAGGACTATAC
GATGCTGAGCACGCCCCAGCCGGTGGCCGATCCCAAGAAGGTGGAAGTGATCGAATTCTTCTCCTACCACTGCATCCATT
GCTATGACGACGATCCGGCCTTCAACGCCTGGTCCAAGACTCTGCCGGCCGACGTAAGCTTCCGCAAGGAGCAGATCGTG
TGGCAGAAGTCGATGGAAGGCTTCGCCCGCATGTTCGCCACCTTCAACGCCACCGGCACTTTCGACAAGCTGCACCGCGC
GGCTTTCGACGCGCAGATCAAGCAGCGCGTGGACCTGTCCAAGCCTGAGCAGTTCACTGGCTGGATCAAGCAGCAAAAGG
GCGTGGACAGCGCCAAGCTGCTGCAGACCTATAACTCCTTCGGCATCAACGCCCAGGTGGCGCGCGCGACCAAGATCACC
CGCGACTACCAGATCCAGGGCACGCCGACCGTGATCGTCAACGGCAAGTACGTGGTGGTGACCGCGACGCCGGAACGGAT
GATCCAGGTGATGAATGAACTGGTGGCCAAGGCGCGCGCCGAAAAGAAATAA

Upstream 100 bases:

>100_bases
GATGTGGAGCGCATCCGCGCGCAGCTGAAGCAAGACGGCATCAACGCCAATGTGGTGAAGGCCGAGTAAGTCTCATCTCT
CCAAATAACGGGAAAAGAAC

Downstream 100 bases:

>100_bases
GCGCCAGCGTTATGCGCAAGGGCGGCCCGGATGGCCGCCTTTTTTGTTGGCTGATCGAGCGCAGTGACGGAGTGGTTGAG
GGGGCTGGTTTTTTTGATGT

Product: thiol:disulfide interchange protein dsbA

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 203; Mature: 203

Protein sequence:

>203_residues
MKKWLLLVMLAVSGMANAAIQLGKDYTMLSTPQPVADPKKVEVIEFFSYHCIHCYDDDPAFNAWSKTLPADVSFRKEQIV
WQKSMEGFARMFATFNATGTFDKLHRAAFDAQIKQRVDLSKPEQFTGWIKQQKGVDSAKLLQTYNSFGINAQVARATKIT
RDYQIQGTPTVIVNGKYVVVTATPERMIQVMNELVAKARAEKK

Sequences:

>Translated_203_residues
MKKWLLLVMLAVSGMANAAIQLGKDYTMLSTPQPVADPKKVEVIEFFSYHCIHCYDDDPAFNAWSKTLPADVSFRKEQIV
WQKSMEGFARMFATFNATGTFDKLHRAAFDAQIKQRVDLSKPEQFTGWIKQQKGVDSAKLLQTYNSFGINAQVARATKIT
RDYQIQGTPTVIVNGKYVVVTATPERMIQVMNELVAKARAEKK
>Mature_203_residues
MKKWLLLVMLAVSGMANAAIQLGKDYTMLSTPQPVADPKKVEVIEFFSYHCIHCYDDDPAFNAWSKTLPADVSFRKEQIV
WQKSMEGFARMFATFNATGTFDKLHRAAFDAQIKQRVDLSKPEQFTGWIKQQKGVDSAKLLQTYNSFGINAQVARATKIT
RDYQIQGTPTVIVNGKYVVVTATPERMIQVMNELVAKARAEKK

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 thioredoxin domain [H]

Homologues:

Organism=Escherichia coli, GI1790291, Length=184, Percent_Identity=30.9782608695652, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 22946; Mature: 22946

Theoretical pI: Translated: 9.98; Mature: 9.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKWLLLVMLAVSGMANAAIQLGKDYTMLSTPQPVADPKKVEVIEFFSYHCIHCYDDDPA
CCHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCCCCCCHHHHHEECCEEEEEEECCCCC
FNAWSKTLPADVSFRKEQIVWQKSMEGFARMFATFNATGTFDKLHRAAFDAQIKQRVDLS
CCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
KPEQFTGWIKQQKGVDSAKLLQTYNSFGINAQVARATKITRDYQIQGTPTVIVNGKYVVV
CCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCEEEECCEEEEE
TATPERMIQVMNELVAKARAEKK
EECHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKWLLLVMLAVSGMANAAIQLGKDYTMLSTPQPVADPKKVEVIEFFSYHCIHCYDDDPA
CCHHHHHHHHHHCCHHHHHHHCCCCEEEECCCCCCCCCCHHHHHEECCEEEEEEECCCCC
FNAWSKTLPADVSFRKEQIVWQKSMEGFARMFATFNATGTFDKLHRAAFDAQIKQRVDLS
CCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCC
KPEQFTGWIKQQKGVDSAKLLQTYNSFGINAQVARATKITRDYQIQGTPTVIVNGKYVVV
CCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCEEECCCCEEEECCEEEEE
TATPERMIQVMNELVAKARAEKK
EECHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10051655 [H]