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The map label for this gene is asnB [H]

Identifier: 34499414

GI number: 34499414

Start: 4279442

End: 4281430

Strand: Reverse

Name: asnB [H]

Synonym: CV_3959

Alternate gene names: 34499414

Gene position: 4281430-4279442 (Counterclockwise)

Preceding gene: 34499415

Following gene: 34499413

Centisome position: 90.11

GC content: 70.34

Gene sequence:

>1989_bases
ATGTGCGGAATCGCGGGAGCGCTGCTGGACCGCCATGCGCACGATGGCGACGTGGCCGAGGCCAGGCGGATGATACTGCG
CCAGACCCATCGCGGCCCGGACGGCCTGGCGGCGCGGCGCGTCGGCCCGGCGTTGCTGGCCTCGTCCAGGCTGGCCATCG
TCGATGTCGCCAACGGCAGCCAGCCTTTCAGCGACGAGAGCCGCGCCATCCACGCCGTGTTCAACGGCGAAATCTACAAC
CACCTCGAATTGCGCGAAAACCTGTCGCGCCGCGGCCATAGCCTGTCAGGCGGCAGCGACGGCGAAGTCATCGTCCACCT
GTACGAGGAGTACGGAACGGATTGCTTCCAATGGCTGGACGGCCAGTTCGCCATCGCCATCTTCGACTCCCGCGACGACT
CGCTGCATCTGGCGCGCGACCGAATGGGCATCTGTCCCTTGCATTGGGCGCGGCTGGACGGCGCGCTGTATTTCAGCTCC
GAGATCAAGGGCCTGCTGGCGGTGGCCGGTATGCCGCGCCGGGCGGAGCCGCGCGCGATGCTGCAGCTCGCTTATTTCGG
CGCCGTCTGCGCGCCGCTGACGCCCTTCGGCGGCATCCATCAATTGCCCGCCGCCCACAGGCTCAGCTTCCGCGCCGGAC
GGCTGGAGATTGGCCGTTACTGGTCGCTGGATTTTCCGCTGGCCGGCGAGCATGCCAGGCTGAGCGAGGCGCAGGCGGCC
GAGGGGCTGGCGGCTCGGCTGGAACGGGCCGTGGCCAGCCACGCTCAGGGCGAGTTCGAGCCGACCTGCTTTCTCAGCGG
CGGCATCGATTCGGCCGTGATCGCCGCGCTGCTGCAGCGCCGCAGCCAAGCATCCATCGCCGCTTTCTGCGCCGCATCCG
AACATGGGCGGATGGACGAGGGCGGCGCCGCGGCCCGCACCGCCGAGTGGCTGGGCGTGGATTTGAAGCGGGTCCGCATC
GACGAGGCGGGCATCGCCGACGCCTTCCCCCGGCTGATCTGGCATGGCGAGACGCCGGTGATCTCCACCGAGGCGTCGGC
CCTGATGCGCTTGGCCGGCGAAGCCGGCAAGCGTTCGAAGATCGTGCTCACCGGCGAGGGCGCCGACGAGGCTTTCGCCG
GCTATCTGGCCTTCCGCCAGTACCGGCAGCTGGGCGCGCTGACGGGGCGCGGCCTCTCGCCGTTGCGGACGATGGTCCGG
CCGTGGCTGCGCGGCCATTACGGCTCGGAATGCCTGTTGCCGGGGGAGGACCGGCTGGGCGTGTTGGCCGAGCATTTCGG
CTGCGTGCCGGCGCAGGCCTATGAGTGGGAGTTCTACCGCGAGGCGTTGACGCCGCTGTTTTCCCCGGAGCTGAGGGCGA
TGGCGGCGGCGGGCGGCTGCTGGGACGGCTTCGAGTTCGACCGCGGCGCGGTGCGCGGCCGCCATTGGCTGGACCGCTCG
CTGTACGTGTCCTACCAGGTGATGCTGCCCAATTATCTGCTGGGCGCGCATGGCGACCGCATCTATGCCGCCCATTCGGT
CGAGGGGCGCTACCCCTTTCTGGATCGTGCGCTGGTCGAGTACGCGGCGGCGCTGGATCCGGCGCTGAAGCTGCGCCGCG
GGCGGGAGAAACACATCCTGCGCGTCGCCGCGGCGCGCTGGCTGCCTGACGAGGTGGCCTGGCGGCCGAAAACCCGTTTC
GTGATGCCCTTCGGCACCCCTTTCGTCGGCCCGCGCGCCGCGCCGCTGTACGCGGAGCTGCTGTCGCCGGGCAAGCTGGC
CGAGTACGGCTATTTCGATCCGGAGCGAGTGCGTTCGCTGTTCCGGGCGCTGGCCTCGCCGCCGTCTGCCGGCGGCAAGG
CCCGGCGCTATCTCGAGCGGCTGGCGCTGGGCCTGGGCGCCACCTTCGTGGTCAGCGCCCAGCTCTGGCACCACCTGCAT
TTGGCCGATGGCGGATCGGCCGTGTCCGGAGAGGCGGCGCGGCGCTCCGCGCCGGCGACCATCGCATGA

Upstream 100 bases:

>100_bases
GTGGTGGATGGCAGGGTGGCCGGCAACCTCAACGGCGCGCTGGATGGTTCGCCGCTGCAGGGCACGCGGCTGCTGTCCGG
CCAGGCGGGAGGGTAGGCGC

Downstream 100 bases:

>100_bases
GCAAGGAGCCGTTGATGAACGCAACAGCGCACGATGCGCAGCGCATGCCGGCCGAACTGGAGCGGCTGCGGGGCAGGATA
GACGAATGCATCGCGGCGGC

Product: asparagine synthetase B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 662; Mature: 662

Protein sequence:

>662_residues
MCGIAGALLDRHAHDGDVAEARRMILRQTHRGPDGLAARRVGPALLASSRLAIVDVANGSQPFSDESRAIHAVFNGEIYN
HLELRENLSRRGHSLSGGSDGEVIVHLYEEYGTDCFQWLDGQFAIAIFDSRDDSLHLARDRMGICPLHWARLDGALYFSS
EIKGLLAVAGMPRRAEPRAMLQLAYFGAVCAPLTPFGGIHQLPAAHRLSFRAGRLEIGRYWSLDFPLAGEHARLSEAQAA
EGLAARLERAVASHAQGEFEPTCFLSGGIDSAVIAALLQRRSQASIAAFCAASEHGRMDEGGAAARTAEWLGVDLKRVRI
DEAGIADAFPRLIWHGETPVISTEASALMRLAGEAGKRSKIVLTGEGADEAFAGYLAFRQYRQLGALTGRGLSPLRTMVR
PWLRGHYGSECLLPGEDRLGVLAEHFGCVPAQAYEWEFYREALTPLFSPELRAMAAAGGCWDGFEFDRGAVRGRHWLDRS
LYVSYQVMLPNYLLGAHGDRIYAAHSVEGRYPFLDRALVEYAAALDPALKLRRGREKHILRVAAARWLPDEVAWRPKTRF
VMPFGTPFVGPRAAPLYAELLSPGKLAEYGYFDPERVRSLFRALASPPSAGGKARRYLERLALGLGATFVVSAQLWHHLH
LADGGSAVSGEAARRSAPATIA

Sequences:

>Translated_662_residues
MCGIAGALLDRHAHDGDVAEARRMILRQTHRGPDGLAARRVGPALLASSRLAIVDVANGSQPFSDESRAIHAVFNGEIYN
HLELRENLSRRGHSLSGGSDGEVIVHLYEEYGTDCFQWLDGQFAIAIFDSRDDSLHLARDRMGICPLHWARLDGALYFSS
EIKGLLAVAGMPRRAEPRAMLQLAYFGAVCAPLTPFGGIHQLPAAHRLSFRAGRLEIGRYWSLDFPLAGEHARLSEAQAA
EGLAARLERAVASHAQGEFEPTCFLSGGIDSAVIAALLQRRSQASIAAFCAASEHGRMDEGGAAARTAEWLGVDLKRVRI
DEAGIADAFPRLIWHGETPVISTEASALMRLAGEAGKRSKIVLTGEGADEAFAGYLAFRQYRQLGALTGRGLSPLRTMVR
PWLRGHYGSECLLPGEDRLGVLAEHFGCVPAQAYEWEFYREALTPLFSPELRAMAAAGGCWDGFEFDRGAVRGRHWLDRS
LYVSYQVMLPNYLLGAHGDRIYAAHSVEGRYPFLDRALVEYAAALDPALKLRRGREKHILRVAAARWLPDEVAWRPKTRF
VMPFGTPFVGPRAAPLYAELLSPGKLAEYGYFDPERVRSLFRALASPPSAGGKARRYLERLALGLGATFVVSAQLWHHLH
LADGGSAVSGEAARRSAPATIA
>Mature_662_residues
MCGIAGALLDRHAHDGDVAEARRMILRQTHRGPDGLAARRVGPALLASSRLAIVDVANGSQPFSDESRAIHAVFNGEIYN
HLELRENLSRRGHSLSGGSDGEVIVHLYEEYGTDCFQWLDGQFAIAIFDSRDDSLHLARDRMGICPLHWARLDGALYFSS
EIKGLLAVAGMPRRAEPRAMLQLAYFGAVCAPLTPFGGIHQLPAAHRLSFRAGRLEIGRYWSLDFPLAGEHARLSEAQAA
EGLAARLERAVASHAQGEFEPTCFLSGGIDSAVIAALLQRRSQASIAAFCAASEHGRMDEGGAAARTAEWLGVDLKRVRI
DEAGIADAFPRLIWHGETPVISTEASALMRLAGEAGKRSKIVLTGEGADEAFAGYLAFRQYRQLGALTGRGLSPLRTMVR
PWLRGHYGSECLLPGEDRLGVLAEHFGCVPAQAYEWEFYREALTPLFSPELRAMAAAGGCWDGFEFDRGAVRGRHWLDRS
LYVSYQVMLPNYLLGAHGDRIYAAHSVEGRYPFLDRALVEYAAALDPALKLRRGREKHILRVAAARWLPDEVAWRPKTRF
VMPFGTPFVGPRAAPLYAELLSPGKLAEYGYFDPERVRSLFRALASPPSAGGKARRYLERLALGLGATFVVSAQLWHHLH
LADGGSAVSGEAARRSAPATIA

Specific function: Main asparagine synthetase in vegetative cells [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI296010848, Length=376, Percent_Identity=26.8617021276596, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI168229248, Length=376, Percent_Identity=26.8617021276596, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI168229252, Length=376, Percent_Identity=26.8617021276596, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI168229250, Length=376, Percent_Identity=26.8617021276596, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI296010852, Length=298, Percent_Identity=26.8456375838926, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI296010850, Length=298, Percent_Identity=26.8456375838926, Blast_Score=77, Evalue=5e-14,
Organism=Escherichia coli, GI1786889, Length=401, Percent_Identity=32.4189526184539, Blast_Score=139, Evalue=7e-34,
Organism=Caenorhabditis elegans, GI71993933, Length=399, Percent_Identity=30.3258145363409, Blast_Score=119, Evalue=5e-27,
Organism=Caenorhabditis elegans, GI25147557, Length=399, Percent_Identity=30.3258145363409, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI25147560, Length=328, Percent_Identity=28.3536585365854, Blast_Score=86, Evalue=5e-17,
Organism=Saccharomyces cerevisiae, GI6321563, Length=418, Percent_Identity=29.6650717703349, Blast_Score=128, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6325403, Length=417, Percent_Identity=28.0575539568345, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI45553209, Length=401, Percent_Identity=30.9226932668329, Blast_Score=139, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 72315; Mature: 72315

Theoretical pI: Translated: 8.03; Mature: 8.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGIAGALLDRHAHDGDVAEARRMILRQTHRGPDGLAARRVGPALLASSRLAIVDVANGS
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHCCCEEEEEECCCC
QPFSDESRAIHAVFNGEIYNHLELRENLSRRGHSLSGGSDGEVIVHLYEEYGTDCFQWLD
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHCCHHHHHCC
GQFAIAIFDSRDDSLHLARDRMGICPLHWARLDGALYFSSEIKGLLAVAGMPRRAEPRAM
CCEEEEEECCCCCCEEEHHHCCCCCCHHHHHHCCEEEEHHHHHHHHHHCCCCCCCCHHHH
LQLAYFGAVCAPLTPFGGIHQLPAAHRLSFRAGRLEIGRYWSLDFPLAGEHARLSEAQAA
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCEEECCEEECCCCCCCCCHHHHHHHHH
EGLAARLERAVASHAQGEFEPTCFLSGGIDSAVIAALLQRRSQASIAAFCAASEHGRMDE
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCCC
GGAAARTAEWLGVDLKRVRIDEAGIADAFPRLIWHGETPVISTEASALMRLAGEAGKRSK
CCCHHHHHHHHCCCHHEEEECCCCHHHHHHHHEECCCCCEEEHHHHHHHHHHHCCCCCCE
IVLTGEGADEAFAGYLAFRQYRQLGALTGRGLSPLRTMVRPWLRGHYGSECLLPGEDRLG
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHH
VLAEHFGCVPAQAYEWEFYREALTPLFSPELRAMAAAGGCWDGFEFDRGAVRGRHWLDRS
HHHHHCCCCCCCHHHHHHHHHHHCHHCCHHHHHHHHCCCCCCCCCCCCCHHCCHHHHCCE
LYVSYQVMLPNYLLGAHGDRIYAAHSVEGRYPFLDRALVEYAAALDPALKLRRGREKHIL
EEEEEEEECCCHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCHHHHHHCCCHHHHH
RVAAARWLPDEVAWRPKTRFVMPFGTPFVGPRAAPLYAELLSPGKLAEYGYFDPERVRSL
HHHHHHCCCHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHH
FRALASPPSAGGKARRYLERLALGLGATFVVSAQLWHHLHLADGGSAVSGEAARRSAPAT
HHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCCCCCHHHHCCCCCC
IA
CC
>Mature Secondary Structure
MCGIAGALLDRHAHDGDVAEARRMILRQTHRGPDGLAARRVGPALLASSRLAIVDVANGS
CCCHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHCCHHHHHCCCEEEEEECCCC
QPFSDESRAIHAVFNGEIYNHLELRENLSRRGHSLSGGSDGEVIVHLYEEYGTDCFQWLD
CCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEHHHHCCHHHHHCC
GQFAIAIFDSRDDSLHLARDRMGICPLHWARLDGALYFSSEIKGLLAVAGMPRRAEPRAM
CCEEEEEECCCCCCEEEHHHCCCCCCHHHHHHCCEEEEHHHHHHHHHHCCCCCCCCHHHH
LQLAYFGAVCAPLTPFGGIHQLPAAHRLSFRAGRLEIGRYWSLDFPLAGEHARLSEAQAA
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCEEECCEEECCCCCCCCCHHHHHHHHH
EGLAARLERAVASHAQGEFEPTCFLSGGIDSAVIAALLQRRSQASIAAFCAASEHGRMDE
HHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCCCC
GGAAARTAEWLGVDLKRVRIDEAGIADAFPRLIWHGETPVISTEASALMRLAGEAGKRSK
CCCHHHHHHHHCCCHHEEEECCCCHHHHHHHHEECCCCCEEEHHHHHHHHHHHCCCCCCE
IVLTGEGADEAFAGYLAFRQYRQLGALTGRGLSPLRTMVRPWLRGHYGSECLLPGEDRLG
EEEECCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCHH
VLAEHFGCVPAQAYEWEFYREALTPLFSPELRAMAAAGGCWDGFEFDRGAVRGRHWLDRS
HHHHHCCCCCCCHHHHHHHHHHHCHHCCHHHHHHHHCCCCCCCCCCCCCHHCCHHHHCCE
LYVSYQVMLPNYLLGAHGDRIYAAHSVEGRYPFLDRALVEYAAALDPALKLRRGREKHIL
EEEEEEEECCCHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCHHHHHHCCCHHHHH
RVAAARWLPDEVAWRPKTRFVMPFGTPFVGPRAAPLYAELLSPGKLAEYGYFDPERVRSL
HHHHHHCCCHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHH
FRALASPPSAGGKARRYLERLALGLGATFVVSAQLWHHLHLADGGSAVSGEAARRSAPAT
HHHHCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHEEEECCCCCCCCCHHHHCCCCCC
IA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9387221; 9384377; 8755891; 10498721 [H]