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The map label for this gene is nadB [H]

Identifier: 34499385

GI number: 34499385

Start: 4250379

End: 4251968

Strand: Direct

Name: nadB [H]

Synonym: CV_3930

Alternate gene names: 34499385

Gene position: 4250379-4251968 (Clockwise)

Preceding gene: 34499384

Following gene: 34499386

Centisome position: 89.46

GC content: 63.52

Gene sequence:

>1590_bases
ATGCTCAAGTTTGATGTGCTGATTATCGGCAGCGGCCTGGCTGGGATGACGCTGGCGCTGCACCTGGCGGAAACGCGCAA
GGTAGGATTGATCACCAAGCGGGATCTGCTGGAAGGCAACTCCACCTACGCCCAGGGCGGCATCGCCGCGGTACTGGATA
CCGAAGACTCGGTAGAAGACCATATCCGCGACACGCTGGTCGCTGGCGCGGGACTATGCAATGAGAAGGCGACCCGCTTC
ATCGTCGAACACTCCAAGGAGGCGATAGACTGGCTGATCAAGCTGGGTGTGCCCTTCACCCGCGACGACGAACACCAGAC
CGGCTATCACCTGACTCGAGAAGGCGGCCACAGCCACCGCCGCATCATCCACGCCGCCGACGCCACCGGCGCCGCCGTGA
CCAGCACGCTGGGACAGAAGATCAAGGCCCATCCCAACATCACCGAGCTGGAACACTATATAGCGATCGACCTGATCACC
TCGCAGAAGCTGGGCCAGTCCGGCAAGCGCGCCTATGGCGTGTACGCGCTGGACAAGAATGGCGACGAAGTCGTCACCAT
CGCCGCCCAACACACCATCCTCGCCTCCGGCGGCGCCGGCAAGGTTTACCTTTATACCACCAATCCCGACACCGCCACCG
GCGACGGCATCGCGATGGGCTGGCGCGCAGGTTGCCGGGTGGGCAATATGGAATTCATCCAGTTCCATCCCACCTGCCTC
TACCACCCGCACGCCAAGTCCTTCCTGATATCGGAAGCCGTGCGCGGCGAGGGCGGCATCCTGAAGCTGCCGGATGGCAC
CCGTTTCATGCCGCAGCACGATCCGCGCGGCGAGCTGGCACCGCGCGACGTGGTGGCGCGCGCGATCGACTTCGAGATGA
AAAAGCACGGCCTGGATTGCGTCTACCTGGATATTTCCTATAAACCGGCCCAGTTCATCAAGGAACACTTCCCCAATATC
TACGCCCACTGCCTGGAACTGGGCATAGACATCACCCAGCAACCAATACCGGTGGTGCCTGCGGTGCATTTCACCTGCGG
CGGCATCGTCACCGACCTGGCCGGCCGCACCGATGTGGACGGACTGTACGCGGTGGGCGAAGTCGCCTGTACCGGCCTGC
ACGGCGCCAACCGCCTGGCCAGCAACTCCTTGCTGGAATGCCTGGTGATCGGCAAGGCGGCCGCGGAGGACATCCTGGCG
GCGGACAGCATCAAATTGCCGGCCATCCCGGCCTGGGACGACAGCCGCGTCACCGATCCGGACGAAGAGGTGGTGATCTC
CCACAACTGGGACGAACTGCGCCGCGCGATGTGGGACTACGTCGGCATCGTCCGCACCAATAAGCGGCTGGAGCGCGCGC
TGCACCGGATCGACCTGCTGAAAGGCGAGATCGACGAGTATTACAGCCACTTCCACGTCAGCAACGACCTGATCGAACTG
CGCAACCTGGTGCAGACCGCCGAACTGATCGTCCGCTCCGCGCTGCTGCGCAAGGAAAGCCGAGGCCTGCATTACAGCCG
TGACTACCCCAACCTGCTGCCCGAGGCGGCGGAAACCGTGCTGGCGCCGGATGCGCCGCTCGGCGGCTGA

Upstream 100 bases:

>100_bases
GAAACCCGGGAATTGAGCGGCCCGCTACGCGCCCGCCCGCGATTCTGCTACGCTCACATGCATTTGGCACGCATGGTGCC
TGCCGTGGAACTCTCTATAT

Downstream 100 bases:

>100_bases
GCCGCTTCCCTGATCCGAACAGCCGCCTGCGGGCGGCTTTTTTCATGTTCGCCGAAACTCGTACAGACACCCATGCAACA
CAATGGCATGAAAATTTAAA

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO 1; Quinolinate synthase B 1 [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MLKFDVLIIGSGLAGMTLALHLAETRKVGLITKRDLLEGNSTYAQGGIAAVLDTEDSVEDHIRDTLVAGAGLCNEKATRF
IVEHSKEAIDWLIKLGVPFTRDDEHQTGYHLTREGGHSHRRIIHAADATGAAVTSTLGQKIKAHPNITELEHYIAIDLIT
SQKLGQSGKRAYGVYALDKNGDEVVTIAAQHTILASGGAGKVYLYTTNPDTATGDGIAMGWRAGCRVGNMEFIQFHPTCL
YHPHAKSFLISEAVRGEGGILKLPDGTRFMPQHDPRGELAPRDVVARAIDFEMKKHGLDCVYLDISYKPAQFIKEHFPNI
YAHCLELGIDITQQPIPVVPAVHFTCGGIVTDLAGRTDVDGLYAVGEVACTGLHGANRLASNSLLECLVIGKAAAEDILA
ADSIKLPAIPAWDDSRVTDPDEEVVISHNWDELRRAMWDYVGIVRTNKRLERALHRIDLLKGEIDEYYSHFHVSNDLIEL
RNLVQTAELIVRSALLRKESRGLHYSRDYPNLLPEAAETVLAPDAPLGG

Sequences:

>Translated_529_residues
MLKFDVLIIGSGLAGMTLALHLAETRKVGLITKRDLLEGNSTYAQGGIAAVLDTEDSVEDHIRDTLVAGAGLCNEKATRF
IVEHSKEAIDWLIKLGVPFTRDDEHQTGYHLTREGGHSHRRIIHAADATGAAVTSTLGQKIKAHPNITELEHYIAIDLIT
SQKLGQSGKRAYGVYALDKNGDEVVTIAAQHTILASGGAGKVYLYTTNPDTATGDGIAMGWRAGCRVGNMEFIQFHPTCL
YHPHAKSFLISEAVRGEGGILKLPDGTRFMPQHDPRGELAPRDVVARAIDFEMKKHGLDCVYLDISYKPAQFIKEHFPNI
YAHCLELGIDITQQPIPVVPAVHFTCGGIVTDLAGRTDVDGLYAVGEVACTGLHGANRLASNSLLECLVIGKAAAEDILA
ADSIKLPAIPAWDDSRVTDPDEEVVISHNWDELRRAMWDYVGIVRTNKRLERALHRIDLLKGEIDEYYSHFHVSNDLIEL
RNLVQTAELIVRSALLRKESRGLHYSRDYPNLLPEAAETVLAPDAPLGG
>Mature_529_residues
MLKFDVLIIGSGLAGMTLALHLAETRKVGLITKRDLLEGNSTYAQGGIAAVLDTEDSVEDHIRDTLVAGAGLCNEKATRF
IVEHSKEAIDWLIKLGVPFTRDDEHQTGYHLTREGGHSHRRIIHAADATGAAVTSTLGQKIKAHPNITELEHYIAIDLIT
SQKLGQSGKRAYGVYALDKNGDEVVTIAAQHTILASGGAGKVYLYTTNPDTATGDGIAMGWRAGCRVGNMEFIQFHPTCL
YHPHAKSFLISEAVRGEGGILKLPDGTRFMPQHDPRGELAPRDVVARAIDFEMKKHGLDCVYLDISYKPAQFIKEHFPNI
YAHCLELGIDITQQPIPVVPAVHFTCGGIVTDLAGRTDVDGLYAVGEVACTGLHGANRLASNSLLECLVIGKAAAEDILA
ADSIKLPAIPAWDDSRVTDPDEEVVISHNWDELRRAMWDYVGIVRTNKRLERALHRIDLLKGEIDEYYSHFHVSNDLIEL
RNLVQTAELIVRSALLRKESRGLHYSRDYPNLLPEAAETVLAPDAPLGG

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=522, Percent_Identity=32.7586206896552, Blast_Score=223, Evalue=3e-58,
Organism=Escherichia coli, GI1788928, Length=531, Percent_Identity=60.6403013182674, Blast_Score=662, Evalue=0.0,
Organism=Escherichia coli, GI1790597, Length=522, Percent_Identity=32.5670498084291, Blast_Score=234, Evalue=2e-62,
Organism=Escherichia coli, GI1786942, Length=559, Percent_Identity=32.5581395348837, Blast_Score=211, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI17550100, Length=538, Percent_Identity=31.5985130111524, Blast_Score=220, Evalue=1e-57,
Organism=Caenorhabditis elegans, GI17505833, Length=540, Percent_Identity=32.037037037037, Blast_Score=218, Evalue=4e-57,
Organism=Caenorhabditis elegans, GI71986328, Length=455, Percent_Identity=27.2527472527473, Blast_Score=102, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6322701, Length=558, Percent_Identity=33.3333333333333, Blast_Score=229, Evalue=8e-61,
Organism=Saccharomyces cerevisiae, GI6322416, Length=557, Percent_Identity=32.6750448833034, Blast_Score=229, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6322511, Length=486, Percent_Identity=28.6008230452675, Blast_Score=125, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320788, Length=483, Percent_Identity=29.6066252587992, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24663005, Length=574, Percent_Identity=32.2299651567944, Blast_Score=223, Evalue=2e-58,
Organism=Drosophila melanogaster, GI17137288, Length=534, Percent_Identity=31.2734082397004, Blast_Score=215, Evalue=5e-56,
Organism=Drosophila melanogaster, GI24655642, Length=534, Percent_Identity=31.2734082397004, Blast_Score=215, Evalue=5e-56,
Organism=Drosophila melanogaster, GI24655647, Length=534, Percent_Identity=31.2734082397004, Blast_Score=215, Evalue=5e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 57896; Mature: 57896

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKFDVLIIGSGLAGMTLALHLAETRKVGLITKRDLLEGNSTYAQGGIAAVLDTEDSVED
CEEEEEEEEECCCHHHHHHEEHHHCCCCCEEEHHHHHCCCCCCCCCCEEEEECCCHHHHH
HIRDTLVAGAGLCNEKATRFIVEHSKEAIDWLIKLGVPFTRDDEHQTGYHLTREGGHSHR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCE
RIIHAADATGAAVTSTLGQKIKAHPNITELEHYIAIDLITSQKLGQSGKRAYGVYALDKN
EEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHEEEEEEEECHHCCCCCCCEEEEEEECCC
GDEVVTIAAQHTILASGGAGKVYLYTTNPDTATGDGIAMGWRAGCRVGNMEFIQFHPTCL
CCEEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCEEECHHCCCEECCEEEEEECCEEE
YHPHAKSFLISEAVRGEGGILKLPDGTRFMPQHDPRGELAPRDVVARAIDFEMKKHGLDC
ECCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCE
VYLDISYKPAQFIKEHFPNIYAHCLELGIDITQQPIPVVPAVHFTCGGIVTDLAGRTDVD
EEEEECCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCHHHHHHCCCCCCC
GLYAVGEVACTGLHGANRLASNSLLECLVIGKAAAEDILAADSIKLPAIPAWDDSRVTDP
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
DEEVVISHNWDELRRAMWDYVGIVRTNKRLERALHRIDLLKGEIDEYYSHFHVSNDLIEL
CCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
RNLVQTAELIVRSALLRKESRGLHYSRDYPNLLPEAAETVLAPDAPLGG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MLKFDVLIIGSGLAGMTLALHLAETRKVGLITKRDLLEGNSTYAQGGIAAVLDTEDSVED
CEEEEEEEEECCCHHHHHHEEHHHCCCCCEEEHHHHHCCCCCCCCCCEEEEECCCHHHHH
HIRDTLVAGAGLCNEKATRFIVEHSKEAIDWLIKLGVPFTRDDEHQTGYHLTREGGHSHR
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCE
RIIHAADATGAAVTSTLGQKIKAHPNITELEHYIAIDLITSQKLGQSGKRAYGVYALDKN
EEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHEEEEEEEECHHCCCCCCCEEEEEEECCC
GDEVVTIAAQHTILASGGAGKVYLYTTNPDTATGDGIAMGWRAGCRVGNMEFIQFHPTCL
CCEEEEEEEEEEEEEECCCCEEEEEECCCCCCCCCCEEECHHCCCEECCEEEEEECCEEE
YHPHAKSFLISEAVRGEGGILKLPDGTRFMPQHDPRGELAPRDVVARAIDFEMKKHGLDC
ECCCHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCE
VYLDISYKPAQFIKEHFPNIYAHCLELGIDITQQPIPVVPAVHFTCGGIVTDLAGRTDVD
EEEEECCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHCHHHHHHCCCCCCC
GLYAVGEVACTGLHGANRLASNSLLECLVIGKAAAEDILAADSIKLPAIPAWDDSRVTDP
CHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
DEEVVISHNWDELRRAMWDYVGIVRTNKRLERALHRIDLLKGEIDEYYSHFHVSNDLIEL
CCCEEEECCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
RNLVQTAELIVRSALLRKESRGLHYSRDYPNLLPEAAETVLAPDAPLGG
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]