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The map label for this gene is narK [H]

Identifier: 34499362

GI number: 34499362

Start: 4218537

End: 4219916

Strand: Reverse

Name: narK [H]

Synonym: CV_3907

Alternate gene names: 34499362

Gene position: 4219916-4218537 (Counterclockwise)

Preceding gene: 34499363

Following gene: 34499361

Centisome position: 88.82

GC content: 65.65

Gene sequence:

>1380_bases
ATGACCGTAAGCATCAAGCGTTGGGAGCCGGAAAACGTCATTTTCTGGCTGAGCCGCGGCCGCGGCATCGCCCGGCGCAA
TCTGTGGCTGTCCGTGTTTTCCCTGCACCTCAACTTCAACATCTGGATGATGTGGAGCATGGTGGTGGTCAATCTGCCGG
CGGTCGGCTTCCTGATCTCCGGCCAGCAGCAGTTCCTGCTGGTGTCGATTCCGCCCTTGGTCGGCGCGCTGATGCGCTTG
ATCTACTCGTGGATCTGGAGCTGGGTGGGCGGCGGCCTATGGCTGGGGCTATCGACGCTATTGCTGCTGCTGCCGGCCTG
GGGCGTAGGCCGGGTGGTGCAGGACATCGCCTCGCCGTTCTGGCAGCTGCTGCTGGTGGCGGGATTGTGCGGCGTAGGCG
GCGGCGCCAGCGCGTCCCACTTGTCCAATACCAGCTTCTTCTTCCCCAGGTCCGAGAAGGGCTTGGCGATGGGGCTCAAC
GCCGGTTTCGGCAACCTGGGCGTGTCGGTGGCGCAGCTGCTGATCCCCTTGTGCATCCTGTATCCGCTGTTCGGCGCTTG
GGCCGGCGAGCCCCAGGTGTGGGGCCGCGGCGGCGAGGTGCATTTCGTCTGGCTGCAGAACGCCGCCTACGTCTGGCTGC
CATTGATCGCGCTGATCGCCGCGGCCTGCCTGCTGTACGCGCACGACCTGCCCAAGCTGCGCATCACGCCGCACGACCAG
CTGGACGCGATGCGCGAGGGGCACACCTGGTATATCTGCTTGCTGTACATGGGCAGCTACGGCACCTTTCTCGGTTTTTC
CGCCGCCTTCCCGCTGCTCGCCCGCGCGATGTTTCCCCAGGTGGACGCCAGCCCCTATCTATTCGTCGGGCCCATGGTGT
GCGCGCTGTCGCGTCCTCTGGGCGGCTGGATGGGCGACCGCGTCGGCGGCGGCGTGGCCACCGTGGCCAGCAATCTGCTG
ATGGCGCTGGGCACCGTCGGCGTCTACCTGAGCCTGCCGTCTGCGGTCAGCGGCGGTTCGTTCTGGCTGTTCCTGGCGAT
GTTCCAGCTGATCTTTTTCGCCGCCGGCGTCGGCAACGGCTCGTCCTACCAGCTGGCGCCCAAGGTCTTCCTGATCCAGG
CCGGCCGCGACGCGCAGCGTCATGGCGAAAGCCTGCAGCAGGCTTATGCCAGAGGAGGCCGACGCGGCGCTTCGGCGATG
AATGTCAGTTCGGTGATGGCGGCCTTCGGCGGTTTCGTGATTCCCAAGGCCTTCGGTTCCTCGCTGAGCTGGTTCGGCAG
CTTTCTGCCGGCATTCGGGATGTTCTTCCTGTTCTATCTGCTGTGCTGCGCGGTGGCATGGTGGCAGTACAGCCGTCCGG
GGGCGGCGATGCGTTGCTGA

Upstream 100 bases:

>100_bases
AAGTCCGCCGGCATGGGCGGATGGCATACTGCGCCGCCTGGCCGACTGGATTGCGTGCGACAGTCCGGACGCGTCGTTCT
GTCATTGTGGAAGTCGAGTC

Downstream 100 bases:

>100_bases
ACCGCGACGACAGGCGCTGGTGGCGGGCTTTGTTGCTGTCCGCCGCCATCCATTTGTCGGCGCTGGCCGCTTGGCTGGCG
CTGGACGGCCGCGCCACGGC

Product: nitrite extrusion protein

Products: NA

Alternate protein names: Nitrite facilitator 1 [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MTVSIKRWEPENVIFWLSRGRGIARRNLWLSVFSLHLNFNIWMMWSMVVVNLPAVGFLISGQQQFLLVSIPPLVGALMRL
IYSWIWSWVGGGLWLGLSTLLLLLPAWGVGRVVQDIASPFWQLLLVAGLCGVGGGASASHLSNTSFFFPRSEKGLAMGLN
AGFGNLGVSVAQLLIPLCILYPLFGAWAGEPQVWGRGGEVHFVWLQNAAYVWLPLIALIAAACLLYAHDLPKLRITPHDQ
LDAMREGHTWYICLLYMGSYGTFLGFSAAFPLLARAMFPQVDASPYLFVGPMVCALSRPLGGWMGDRVGGGVATVASNLL
MALGTVGVYLSLPSAVSGGSFWLFLAMFQLIFFAAGVGNGSSYQLAPKVFLIQAGRDAQRHGESLQQAYARGGRRGASAM
NVSSVMAAFGGFVIPKAFGSSLSWFGSFLPAFGMFFLFYLLCCAVAWWQYSRPGAAMRC

Sequences:

>Translated_459_residues
MTVSIKRWEPENVIFWLSRGRGIARRNLWLSVFSLHLNFNIWMMWSMVVVNLPAVGFLISGQQQFLLVSIPPLVGALMRL
IYSWIWSWVGGGLWLGLSTLLLLLPAWGVGRVVQDIASPFWQLLLVAGLCGVGGGASASHLSNTSFFFPRSEKGLAMGLN
AGFGNLGVSVAQLLIPLCILYPLFGAWAGEPQVWGRGGEVHFVWLQNAAYVWLPLIALIAAACLLYAHDLPKLRITPHDQ
LDAMREGHTWYICLLYMGSYGTFLGFSAAFPLLARAMFPQVDASPYLFVGPMVCALSRPLGGWMGDRVGGGVATVASNLL
MALGTVGVYLSLPSAVSGGSFWLFLAMFQLIFFAAGVGNGSSYQLAPKVFLIQAGRDAQRHGESLQQAYARGGRRGASAM
NVSSVMAAFGGFVIPKAFGSSLSWFGSFLPAFGMFFLFYLLCCAVAWWQYSRPGAAMRC
>Mature_458_residues
TVSIKRWEPENVIFWLSRGRGIARRNLWLSVFSLHLNFNIWMMWSMVVVNLPAVGFLISGQQQFLLVSIPPLVGALMRLI
YSWIWSWVGGGLWLGLSTLLLLLPAWGVGRVVQDIASPFWQLLLVAGLCGVGGGASASHLSNTSFFFPRSEKGLAMGLNA
GFGNLGVSVAQLLIPLCILYPLFGAWAGEPQVWGRGGEVHFVWLQNAAYVWLPLIALIAAACLLYAHDLPKLRITPHDQL
DAMREGHTWYICLLYMGSYGTFLGFSAAFPLLARAMFPQVDASPYLFVGPMVCALSRPLGGWMGDRVGGGVATVASNLLM
ALGTVGVYLSLPSAVSGGSFWLFLAMFQLIFFAAGVGNGSSYQLAPKVFLIQAGRDAQRHGESLQQAYARGGRRGASAMN
VSSVMAAFGGFVIPKAFGSSLSWFGSFLPAFGMFFLFYLLCCAVAWWQYSRPGAAMRC

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=448, Percent_Identity=39.0625, Blast_Score=306, Evalue=1e-84,
Organism=Escherichia coli, GI1787743, Length=448, Percent_Identity=39.5089285714286, Blast_Score=278, Evalue=6e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004737 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 49912; Mature: 49781

Theoretical pI: Translated: 9.63; Mature: 9.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVSIKRWEPENVIFWLSRGRGIARRNLWLSVFSLHLNFNIWMMWSMVVVNLPAVGFLIS
CCEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHEEEC
GQQQFLLVSIPPLVGALMRLIYSWIWSWVGGGLWLGLSTLLLLLPAWGVGRVVQDIASPF
CCCCEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
WQLLLVAGLCGVGGGASASHLSNTSFFFPRSEKGLAMGLNAGFGNLGVSVAQLLIPLCIL
HHHHHHHHHHCCCCCCCHHHCCCCCEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
YPLFGAWAGEPQVWGRGGEVHFVWLQNAAYVWLPLIALIAAACLLYAHDLPKLRITPHDQ
HHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHH
LDAMREGHTWYICLLYMGSYGTFLGFSAAFPLLARAMFPQVDASPYLFVGPMVCALSRPL
HHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCC
GGWMGDRVGGGVATVASNLLMALGTVGVYLSLPSAVSGGSFWLFLAMFQLIFFAAGVGNG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
SSYQLAPKVFLIQAGRDAQRHGESLQQAYARGGRRGASAMNVSSVMAAFGGFVIPKAFGS
CCEEECCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHCC
SLSWFGSFLPAFGMFFLFYLLCCAVAWWQYSRPGAAMRC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
TVSIKRWEPENVIFWLSRGRGIARRNLWLSVFSLHLNFNIWMMWSMVVVNLPAVGFLIS
CEEEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCHHHEEEC
GQQQFLLVSIPPLVGALMRLIYSWIWSWVGGGLWLGLSTLLLLLPAWGVGRVVQDIASPF
CCCCEEEEECCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
WQLLLVAGLCGVGGGASASHLSNTSFFFPRSEKGLAMGLNAGFGNLGVSVAQLLIPLCIL
HHHHHHHHHHCCCCCCCHHHCCCCCEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHH
YPLFGAWAGEPQVWGRGGEVHFVWLQNAAYVWLPLIALIAAACLLYAHDLPKLRITPHDQ
HHHHHCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCHH
LDAMREGHTWYICLLYMGSYGTFLGFSAAFPLLARAMFPQVDASPYLFVGPMVCALSRPL
HHHHHCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCC
GGWMGDRVGGGVATVASNLLMALGTVGVYLSLPSAVSGGSFWLFLAMFQLIFFAAGVGNG
CCCCCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCC
SSYQLAPKVFLIQAGRDAQRHGESLQQAYARGGRRGASAMNVSSVMAAFGGFVIPKAFGS
CCEEECCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCHHHHHHCC
SLSWFGSFLPAFGMFFLFYLLCCAVAWWQYSRPGAAMRC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2668029; 8905232; 9278503; 2657652 [H]