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Identifier: 34499358

GI number: 34499358

Start: 4213148

End: 4215613

Strand: Reverse

Name: lig

Synonym: CV_3903

Alternate gene names: 34499358

Gene position: 4215613-4213148 (Counterclockwise)

Preceding gene: 34499359

Following gene: 34499357

Centisome position: 88.73

GC content: 70.24

Gene sequence:

>2466_bases
TTGACGATACCCGGGGCCGGCTTTGCCGGCCCTTTTGCCGTTCGAGAGTTTCCGATGACCGCAAATGAATTGCGCGCAGC
CGAGCTGCGCGAGCTGCTGAACCGCTACGGCCATGAATATTATGTGCTGGACGCGCCGACGGTGCCCGACGCCGAATACG
ACCGCCTGTTCCGCGAGCTGCAGGCGCTGGAAGAGGCGCATCCGGAACTGGCGGTCTCCGATTCGCCGACCCGCCGCGTC
GGCGGCGCGCCGCTGGACGAGTTCGTCAGCGTCGTGCACGCGGTGCCTATGCTGTCCCTGTCCAACGCTTTCTCCGACAT
GCAGCTGACCGACCCGGCCGAGCGCCACTCCGAGCTGATCCAGTTCGACGAGCGGGTGCGCAAGGGACTGGACGCGGCCG
AGGTGGAGTACGCGACCGAACCCAAGTTCGACGGCCTGGCTATCTCCCTGCTGTACGAGAACGGCGTGCTGACCCGCGCC
GCCACCCGCGGCGACGGCGTGGCCGGCGAGCAGGTGACCGAGAACGTGCGCACCATACGCGCGATTCCGCTGAAGCTGGA
TGGCGCGAATCCGCCGGCGCTGCTGGAGGTGCGCGGCGAGGTGCTGATGCTGAAGCGCGACTTCGAGCGGCTGAACGCCG
ATCAGATCGCCCGCGGCGACAAGACCTTCGCCAATCCGCGCAACGCCGCCGCCGGTAGTCTGCGCCAGCTGGATTCGCGC
ATCACCGCGCAGCGCCGGCTGTCGTTCTTCGCTTATTCCATCGCCCAGGTCGGCGGGGCTGACTGGCCGGCGACGCACGC
CGGCGAGATGGCCTGGCTGAAAACGCTGGGTTTTCCGGTGGTCATGGACAGCCTGCGGCCGGTGGTCAGCGGCGCAGCCG
GCCTGGCCGGCTATTACGAGGCGGTGCTGACGGCCCGCGCCGGCCTGCCCTTCGAGATAGACGGCGTGGTGTACAAGGTG
AACCGGCGCGACCAGCAGGAGGCGCTGGGTTTCGTGTCGCGCGCGCCGCGCTGGGCCATCGCCCACAAATTCCCGGCGGA
GGAAGCGCTGACCTGCGTCGAGGCGATCGAGGAGCAGGTGGGCCGCACCGGCGCCATCACGCCGGTGGCGCGGCTGAAGC
CGGTCTTCGTCGGCGGCGTCACCGTCACCAACGCCACGCTGCACAATGAGGACGAGGTGCGGCGCAAGGACGTGCGCGTC
GGCGACACCGTGGTGGTGCGCCGCGCCGGCGACGTGATTCCCGAGGTGGTGTCGGTGGTGCTGGCGCAGCGGCCGATGCA
GCCGGCCGAAGGCGGCGACCTGTTCAGTGCCGGCGAGGAGCCGCGATACCCGGCCTACCGCTTGCCGACCGCCTGCCCGG
TGTGCGGCAGCCACGTGGTGCGCGAGGAGGGCGAGGCCATCGCCCGCTGCTCGGGCGGGCTGTCCTGCCGTGCCCAGCGC
AGCCAGGCGATCCAGCACTTCGCCGGCCGCCGCATGATGGACATTGACGGCCTGGGCGAGCGCTATATCGACAAGCTGGT
CGAGTACGGTTATGTCCAGGGCGTGGCCGACCTGTACCGGCTGAAGCTGGAAGACCTGCTGGAGATGAAGCGCCGCGCCG
ACGAGGACGAGGGCGTGACGCCGGAGACGGTGAAGGCCGGCAAGGTGGCCAGCAAGTGGGCGGAAAACCTGATCGAGGCG
ATAGACGCCAGCCGCGCGCCGCCGCTGGCCCGACTCCTGTTCGCGCTGGGCATCCGCCATGTCGGCGAATCCACCGCCAA
GACGCTGGCCGACTGGCTGGGCACCATGGCGCTGATCCGCCGCTGTCCGGCGGCGTTGTTCGCCGCGCTGCCGGACATCG
GCGGCGTGGTGGCCGATTCGCTGGCCGATTTTTTCGCCGAGGACAACAATGAAAAGGCGCTGGACGCCTTGCTGGCCGAA
GTGAAGCCGGCCGACGAGCATGCGCCGTCGCCCAAGCTGCGCGAGCGGCTGGACGACGCGTCGCTGCTGGCGCGGCTGGC
CATTCCGCGGCTGACCGAGGTGCGCAGCCAGCAACTGGCCGCGCAGCGCAGCCTGGCCTGGCTGGGGCAGAGCGAGCGCC
GCGCGTTGCTGGCGCTGGAACTGCCGGCCGAGGTGGTCAACGCCCTGGCCGACTGGCTGGACGAACCGGGCCGCCGCGCG
GCGCTGGCCAGCCTGGCCGGCCTGCGGGACGAGATCCTTGCCAGCCTGCCGGCCGCGGCCGAGGCGGCGGCGCTGCCGCT
GGAAGGCAAGACGCTGGTGCTGACCGGCACGCTGCCCACGCTGAGCCGCGATCAGGCCAAGGCGCTGATCGAGGCCGCCG
GCGGCAAGGTGTCCGGCAGCGTGTCCAAGAAAACCCACTACGTGGTGGCCGGCGAGGAGGCGGGCGGCAAGCTCGCCAAG
GCGCAGGAACTGGGCGTGGCGATACTGGACGAGGCAGGCCTGCAAGCCTTGCTGGCGGGTAACTGA

Upstream 100 bases:

>100_bases
GCATCCGCGACCAGCTGCTGCACATCTATGGCAGCATGGACGACCGCGGCATCGCGCCGGGCAGCGTGGCGGCCCTGCGC
CTGTTCGCCTGAACGATGAT

Downstream 100 bases:

>100_bases
ACGAGGTTGAGATGACATCTGCTGCGATGGCGACAGCCTATTTCGACAAAGGCAATCAATGCGCGGCCCGCTGCGACTGG
CTGGGCGCGATCGAGGCCTT

Product: DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 821; Mature: 820

Protein sequence:

>821_residues
MTIPGAGFAGPFAVREFPMTANELRAAELRELLNRYGHEYYVLDAPTVPDAEYDRLFRELQALEEAHPELAVSDSPTRRV
GGAPLDEFVSVVHAVPMLSLSNAFSDMQLTDPAERHSELIQFDERVRKGLDAAEVEYATEPKFDGLAISLLYENGVLTRA
ATRGDGVAGEQVTENVRTIRAIPLKLDGANPPALLEVRGEVLMLKRDFERLNADQIARGDKTFANPRNAAAGSLRQLDSR
ITAQRRLSFFAYSIAQVGGADWPATHAGEMAWLKTLGFPVVMDSLRPVVSGAAGLAGYYEAVLTARAGLPFEIDGVVYKV
NRRDQQEALGFVSRAPRWAIAHKFPAEEALTCVEAIEEQVGRTGAITPVARLKPVFVGGVTVTNATLHNEDEVRRKDVRV
GDTVVVRRAGDVIPEVVSVVLAQRPMQPAEGGDLFSAGEEPRYPAYRLPTACPVCGSHVVREEGEAIARCSGGLSCRAQR
SQAIQHFAGRRMMDIDGLGERYIDKLVEYGYVQGVADLYRLKLEDLLEMKRRADEDEGVTPETVKAGKVASKWAENLIEA
IDASRAPPLARLLFALGIRHVGESTAKTLADWLGTMALIRRCPAALFAALPDIGGVVADSLADFFAEDNNEKALDALLAE
VKPADEHAPSPKLRERLDDASLLARLAIPRLTEVRSQQLAAQRSLAWLGQSERRALLALELPAEVVNALADWLDEPGRRA
ALASLAGLRDEILASLPAAAEAAALPLEGKTLVLTGTLPTLSRDQAKALIEAAGGKVSGSVSKKTHYVVAGEEAGGKLAK
AQELGVAILDEAGLQALLAGN

Sequences:

>Translated_821_residues
MTIPGAGFAGPFAVREFPMTANELRAAELRELLNRYGHEYYVLDAPTVPDAEYDRLFRELQALEEAHPELAVSDSPTRRV
GGAPLDEFVSVVHAVPMLSLSNAFSDMQLTDPAERHSELIQFDERVRKGLDAAEVEYATEPKFDGLAISLLYENGVLTRA
ATRGDGVAGEQVTENVRTIRAIPLKLDGANPPALLEVRGEVLMLKRDFERLNADQIARGDKTFANPRNAAAGSLRQLDSR
ITAQRRLSFFAYSIAQVGGADWPATHAGEMAWLKTLGFPVVMDSLRPVVSGAAGLAGYYEAVLTARAGLPFEIDGVVYKV
NRRDQQEALGFVSRAPRWAIAHKFPAEEALTCVEAIEEQVGRTGAITPVARLKPVFVGGVTVTNATLHNEDEVRRKDVRV
GDTVVVRRAGDVIPEVVSVVLAQRPMQPAEGGDLFSAGEEPRYPAYRLPTACPVCGSHVVREEGEAIARCSGGLSCRAQR
SQAIQHFAGRRMMDIDGLGERYIDKLVEYGYVQGVADLYRLKLEDLLEMKRRADEDEGVTPETVKAGKVASKWAENLIEA
IDASRAPPLARLLFALGIRHVGESTAKTLADWLGTMALIRRCPAALFAALPDIGGVVADSLADFFAEDNNEKALDALLAE
VKPADEHAPSPKLRERLDDASLLARLAIPRLTEVRSQQLAAQRSLAWLGQSERRALLALELPAEVVNALADWLDEPGRRA
ALASLAGLRDEILASLPAAAEAAALPLEGKTLVLTGTLPTLSRDQAKALIEAAGGKVSGSVSKKTHYVVAGEEAGGKLAK
AQELGVAILDEAGLQALLAGN
>Mature_820_residues
TIPGAGFAGPFAVREFPMTANELRAAELRELLNRYGHEYYVLDAPTVPDAEYDRLFRELQALEEAHPELAVSDSPTRRVG
GAPLDEFVSVVHAVPMLSLSNAFSDMQLTDPAERHSELIQFDERVRKGLDAAEVEYATEPKFDGLAISLLYENGVLTRAA
TRGDGVAGEQVTENVRTIRAIPLKLDGANPPALLEVRGEVLMLKRDFERLNADQIARGDKTFANPRNAAAGSLRQLDSRI
TAQRRLSFFAYSIAQVGGADWPATHAGEMAWLKTLGFPVVMDSLRPVVSGAAGLAGYYEAVLTARAGLPFEIDGVVYKVN
RRDQQEALGFVSRAPRWAIAHKFPAEEALTCVEAIEEQVGRTGAITPVARLKPVFVGGVTVTNATLHNEDEVRRKDVRVG
DTVVVRRAGDVIPEVVSVVLAQRPMQPAEGGDLFSAGEEPRYPAYRLPTACPVCGSHVVREEGEAIARCSGGLSCRAQRS
QAIQHFAGRRMMDIDGLGERYIDKLVEYGYVQGVADLYRLKLEDLLEMKRRADEDEGVTPETVKAGKVASKWAENLIEAI
DASRAPPLARLLFALGIRHVGESTAKTLADWLGTMALIRRCPAALFAALPDIGGVVADSLADFFAEDNNEKALDALLAEV
KPADEHAPSPKLRERLDDASLLARLAIPRLTEVRSQQLAAQRSLAWLGQSERRALLALELPAEVVNALADWLDEPGRRAA
LASLAGLRDEILASLPAAAEAAALPLEGKTLVLTGTLPTLSRDQAKALIEAAGGKVSGSVSKKTHYVVAGEEAGGKLAKA
QELGVAILDEAGLQALLAGN

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Homo sapiens, GI32528306, Length=84, Percent_Identity=50, Blast_Score=75, Evalue=2e-13,
Organism=Escherichia coli, GI1788750, Length=634, Percent_Identity=49.8422712933754, Blast_Score=582, Evalue=1e-167,
Organism=Escherichia coli, GI87082305, Length=555, Percent_Identity=23.2432432432432, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6324791, Length=92, Percent_Identity=42.3913043478261, Blast_Score=69, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_CHRVO (Q7NR81)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903573.1
- HSSP:   O87703
- ProteinModelPortal:   Q7NR81
- GeneID:   2546947
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3903
- NMPDR:   fig|243365.1.peg.3903
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q7NR81
- ProtClustDB:   CLSK337493
- BioCyc:   CVIO243365:CV_3903-MONOMER
- BRENDA:   6.5.1.2
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 88507; Mature: 88376

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 142-142 BINDING 140-140 BINDING 163-163 BINDING 200-200 BINDING 319-319 BINDING 343-343

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIPGAGFAGPFAVREFPMTANELRAAELRELLNRYGHEYYVLDAPTVPDAEYDRLFREL
CCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHH
QALEEAHPELAVSDSPTRRVGGAPLDEFVSVVHAVPMLSLSNAFSDMQLTDPAERHSELI
HHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
QFDERVRKGLDAAEVEYATEPKFDGLAISLLYENGVLTRAATRGDGVAGEQVTENVRTIR
HHHHHHHCCCCHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHE
AIPLKLDGANPPALLEVRGEVLMLKRDFERLNADQIARGDKTFANPRNAAAGSLRQLDSR
EEEEEECCCCCCEEEEECCCEEEHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHH
ITAQRRLSFFAYSIAQVGGADWPATHAGEMAWLKTLGFPVVMDSLRPVVSGAAGLAGYYE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AVLTARAGLPFEIDGVVYKVNRRDQQEALGFVSRAPRWAIAHKFPAEEALTCVEAIEEQV
HHHHHHCCCCEEECCEEEECCCCCHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHH
GRTGAITPVARLKPVFVGGVTVTNATLHNEDEVRRKDVRVGDTVVVRRAGDVIPEVVSVV
CCCCCCCHHHHHCCEEECCEEEEECEECCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
LAQRPMQPAEGGDLFSAGEEPRYPAYRLPTACPVCGSHVVREEGEAIARCSGGLSCRAQR
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCHHHHH
SQAIQHFAGRRMMDIDGLGERYIDKLVEYGYVQGVADLYRLKLEDLLEMKRRADEDEGVT
HHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PETVKAGKVASKWAENLIEAIDASRAPPLARLLFALGIRHVGESTAKTLADWLGTMALIR
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
RCPAALFAALPDIGGVVADSLADFFAEDNNEKALDALLAEVKPADEHAPSPKLRERLDDA
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCHH
SLLARLAIPRLTEVRSQQLAAQRSLAWLGQSERRALLALELPAEVVNALADWLDEPGRRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHH
ALASLAGLRDEILASLPAAAEAAALPLEGKTLVLTGTLPTLSRDQAKALIEAAGGKVSGS
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCC
VSKKTHYVVAGEEAGGKLAKAQELGVAILDEAGLQALLAGN
CCCCEEEEEECCCCCCCHHHHHHCCCHHCCHHCCHHHHCCC
>Mature Secondary Structure 
TIPGAGFAGPFAVREFPMTANELRAAELRELLNRYGHEYYVLDAPTVPDAEYDRLFREL
CCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHH
QALEEAHPELAVSDSPTRRVGGAPLDEFVSVVHAVPMLSLSNAFSDMQLTDPAERHSELI
HHHHHCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
QFDERVRKGLDAAEVEYATEPKFDGLAISLLYENGVLTRAATRGDGVAGEQVTENVRTIR
HHHHHHHCCCCHHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCCCCCHHHHHHHHHHHE
AIPLKLDGANPPALLEVRGEVLMLKRDFERLNADQIARGDKTFANPRNAAAGSLRQLDSR
EEEEEECCCCCCEEEEECCCEEEHHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHH
ITAQRRLSFFAYSIAQVGGADWPATHAGEMAWLKTLGFPVVMDSLRPVVSGAAGLAGYYE
HHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
AVLTARAGLPFEIDGVVYKVNRRDQQEALGFVSRAPRWAIAHKFPAEEALTCVEAIEEQV
HHHHHHCCCCEEECCEEEECCCCCHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHHH
GRTGAITPVARLKPVFVGGVTVTNATLHNEDEVRRKDVRVGDTVVVRRAGDVIPEVVSVV
CCCCCCCHHHHHCCEEECCEEEEECEECCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHH
LAQRPMQPAEGGDLFSAGEEPRYPAYRLPTACPVCGSHVVREEGEAIARCSGGLSCRAQR
HHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHCCCCCHHHHH
SQAIQHFAGRRMMDIDGLGERYIDKLVEYGYVQGVADLYRLKLEDLLEMKRRADEDEGVT
HHHHHHHHCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
PETVKAGKVASKWAENLIEAIDASRAPPLARLLFALGIRHVGESTAKTLADWLGTMALIR
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHH
RCPAALFAALPDIGGVVADSLADFFAEDNNEKALDALLAEVKPADEHAPSPKLRERLDDA
HHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCHH
SLLARLAIPRLTEVRSQQLAAQRSLAWLGQSERRALLALELPAEVVNALADWLDEPGRRA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHCCCCHHH
ALASLAGLRDEILASLPAAAEAAALPLEGKTLVLTGTLPTLSRDQAKALIEAAGGKVSGS
HHHHHHHHHHHHHHHCCCHHHHHCCCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCC
VSKKTHYVVAGEEAGGKLAKAQELGVAILDEAGLQALLAGN
CCCCEEEEEECCCCCCCHHHHHHCCCHHCCHHCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 14500782