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The map label for this gene is ilvB [H]

Identifier: 34499344

GI number: 34499344

Start: 4196302

End: 4198035

Strand: Reverse

Name: ilvB [H]

Synonym: CV_3889

Alternate gene names: 34499344

Gene position: 4198035-4196302 (Counterclockwise)

Preceding gene: 34499345

Following gene: 34499343

Centisome position: 88.36

GC content: 69.03

Gene sequence:

>1734_bases
ATGCATCTGAACGACGCGCTGGCGCAGCAGCCCGACATCGCCGCCGTCCATTGCCATCATGAGCAGGCCTGCGGCATCGC
CGCCGAGGCCTACGGCCGGCTGGCCGAGCGCACCGGCGTGGCCATGGTGACCACCGGCCCCGGCGCCACCAACATCCTGA
CGCCGGTGGCCGGCGCCTGGATCGAATCGCTGCCGCTGCTGGTGATTTCCGGCCAGGTGAAGCGCGCCGACCTGCTGCAG
GGCAGCGGCCTGCGCCAGAAGGGCGTGCAGGAAGTGGATGTCGTGTCCATGGCCAAGCCGGTGACCAAGTACGCGGTCAC
CGTCACCGATCCGCAAGCCATCCGCCAGCATCTGGAGGAGGCGCTCTATCTGGCGCACAGCGGCCGTCCCGGCCCGGTGT
GGCTGGACATCCCGCTGGACGTGCAGGCCGCGCCCATCGATCCGGCCACGCTGCCGGGCTGGCAACCGGAAGGCGAAACC
GCGCCGGCCGCGCAAGACCATGTCGCCGCCATCGCCGATCTGTTGAAGCAGGCCGAGCGGCCGCTGATCCTGGCCGGCCA
CGGCGTGCGCATTGCCGGCGGCGCCGACGCGCTGCGCCGGCTCAGCGAAACGCTGCAAGTGCCGGTGGCCGCCACCTGGA
ACGCGATGGACCTGATGCCGTGGGACAGCCCGCTGTGCGCCGGCCGTCCCGGCGTGGTGGCGCTGCGTCCCGGCAACTTC
GCGGTGCAGAACTGCGACCTGTTGATCGCCATCGGCACCCGCCTCGACAACATCCTCACCGCCTATAACCCCAAGGGCTT
CGCCCGCGCCGCGCGCAAGGTGGTGGTGGACGTCGACGCCGCCGAGATCGCCAAGCTGGAGATGGAGATCGACCTGCCGT
TGGCGATGGACGCCAAGGCCTGCCTGGAGGGCCTGCTGGCGGCCGTCGAGCGCCTGCCCAAGGCCAAGTGGCAGTCATGG
CTGGACCGCTGCGCCGACTGGAAGCGCCGCTACCCGGTGCAGGACGGCGCGCCGTTCCCGACCTCCGGCCAGATCAGCCA
CTATCACTTCGTCGACGCGCTGTCCGACGCCGCGCCGGAAAACACGCTGATTTCCACCGGCAGCTCCGGCCTGGCGGTCG
AGGTGTTCTACACCGTGTTCCGCAACAAGCCGGGCCAGCGCGTATTTCTGACTTCGGGCCTGGGATCGATGGGCTACGGC
CTGCCGGCGGCGATAGGCGCCTGCCTGGGCAACGGCTCCAGGCCGATGATCGCGTTGGAAAGCGACGGCAGCCTGCAACT
GAACCTGCAGGAGCTTGCGACGCTGAAGGCGCTGCGACTGCCCATCCGCCTGTTCATCATGAACAACAACGGCTACGCCT
CCATCCGCAACACCCAGCGCAATTATTTCGACAGCCGCTATGTCGGCACCGGGCCCGAAGCCGGCGTGCTGATCCCGGAC
CTGCTGGAGCTGGCCGAGGCCTACGGCCTGCCTGCGGTGCGCATCGAACGCGTCGAGGAGCTGGCGGAAGGATTGGCCAA
GGTGATGGCGCATCCGGGACCGATTCTGTGCGACGTGCGGCTGAACGAGAACGAAACGCTGCAGCCCAAGGCCGCCGCGC
TGCCGCAGCCGGACGGCTCCATGCTGTCGATGCCGCTGGAAGACATGTCGCCGCTGCTGCCGCTGGAGCAGCTGCGCGCG
GAAATGCTGATTCCGCTGCTGCCGGCGTCGGAAGCCGCCAAGCGGGGCGTTTGA

Upstream 100 bases:

>100_bases
GAGCATGAGCGCAAAACTTGTCCGCCTGGCGGACTACGTGATGGATTTCCTGGCCGCGCGCGGCGTGCGCCACGTGTTTC
TGCTGCCGGGCGGCGGCGCG

Downstream 100 bases:

>100_bases
GCGATGGCCGCTGCGATCGATCACTGCCGCCTCTGCGCGCAGCCGCTGTTTGATCAGCCCTTGCTGGTGCAGGACGACAT
GCCCGCCGCCGCCCAGGGGC

Product: acetolactate synthase

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS; Vegetative protein 105; VEG105 [H]

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MHLNDALAQQPDIAAVHCHHEQACGIAAEAYGRLAERTGVAMVTTGPGATNILTPVAGAWIESLPLLVISGQVKRADLLQ
GSGLRQKGVQEVDVVSMAKPVTKYAVTVTDPQAIRQHLEEALYLAHSGRPGPVWLDIPLDVQAAPIDPATLPGWQPEGET
APAAQDHVAAIADLLKQAERPLILAGHGVRIAGGADALRRLSETLQVPVAATWNAMDLMPWDSPLCAGRPGVVALRPGNF
AVQNCDLLIAIGTRLDNILTAYNPKGFARAARKVVVDVDAAEIAKLEMEIDLPLAMDAKACLEGLLAAVERLPKAKWQSW
LDRCADWKRRYPVQDGAPFPTSGQISHYHFVDALSDAAPENTLISTGSSGLAVEVFYTVFRNKPGQRVFLTSGLGSMGYG
LPAAIGACLGNGSRPMIALESDGSLQLNLQELATLKALRLPIRLFIMNNNGYASIRNTQRNYFDSRYVGTGPEAGVLIPD
LLELAEAYGLPAVRIERVEELAEGLAKVMAHPGPILCDVRLNENETLQPKAAALPQPDGSMLSMPLEDMSPLLPLEQLRA
EMLIPLLPASEAAKRGV

Sequences:

>Translated_577_residues
MHLNDALAQQPDIAAVHCHHEQACGIAAEAYGRLAERTGVAMVTTGPGATNILTPVAGAWIESLPLLVISGQVKRADLLQ
GSGLRQKGVQEVDVVSMAKPVTKYAVTVTDPQAIRQHLEEALYLAHSGRPGPVWLDIPLDVQAAPIDPATLPGWQPEGET
APAAQDHVAAIADLLKQAERPLILAGHGVRIAGGADALRRLSETLQVPVAATWNAMDLMPWDSPLCAGRPGVVALRPGNF
AVQNCDLLIAIGTRLDNILTAYNPKGFARAARKVVVDVDAAEIAKLEMEIDLPLAMDAKACLEGLLAAVERLPKAKWQSW
LDRCADWKRRYPVQDGAPFPTSGQISHYHFVDALSDAAPENTLISTGSSGLAVEVFYTVFRNKPGQRVFLTSGLGSMGYG
LPAAIGACLGNGSRPMIALESDGSLQLNLQELATLKALRLPIRLFIMNNNGYASIRNTQRNYFDSRYVGTGPEAGVLIPD
LLELAEAYGLPAVRIERVEELAEGLAKVMAHPGPILCDVRLNENETLQPKAAALPQPDGSMLSMPLEDMSPLLPLEQLRA
EMLIPLLPASEAAKRGV
>Mature_577_residues
MHLNDALAQQPDIAAVHCHHEQACGIAAEAYGRLAERTGVAMVTTGPGATNILTPVAGAWIESLPLLVISGQVKRADLLQ
GSGLRQKGVQEVDVVSMAKPVTKYAVTVTDPQAIRQHLEEALYLAHSGRPGPVWLDIPLDVQAAPIDPATLPGWQPEGET
APAAQDHVAAIADLLKQAERPLILAGHGVRIAGGADALRRLSETLQVPVAATWNAMDLMPWDSPLCAGRPGVVALRPGNF
AVQNCDLLIAIGTRLDNILTAYNPKGFARAARKVVVDVDAAEIAKLEMEIDLPLAMDAKACLEGLLAAVERLPKAKWQSW
LDRCADWKRRYPVQDGAPFPTSGQISHYHFVDALSDAAPENTLISTGSSGLAVEVFYTVFRNKPGQRVFLTSGLGSMGYG
LPAAIGACLGNGSRPMIALESDGSLQLNLQELATLKALRLPIRLFIMNNNGYASIRNTQRNYFDSRYVGTGPEAGVLIPD
LLELAEAYGLPAVRIERVEELAEGLAKVMAHPGPILCDVRLNENETLQPKAAALPQPDGSMLSMPLEDMSPLLPLEQLRA
EMLIPLLPASEAAKRGV

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=457, Percent_Identity=25.8205689277899, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI21361361, Length=490, Percent_Identity=25.3061224489796, Blast_Score=116, Evalue=7e-26,
Organism=Escherichia coli, GI1790104, Length=528, Percent_Identity=31.8181818181818, Blast_Score=261, Evalue=9e-71,
Organism=Escherichia coli, GI87081685, Length=547, Percent_Identity=28.7020109689214, Blast_Score=210, Evalue=2e-55,
Organism=Escherichia coli, GI1786717, Length=529, Percent_Identity=30.0567107750473, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI1788716, Length=455, Percent_Identity=29.6703296703297, Blast_Score=145, Evalue=7e-36,
Organism=Escherichia coli, GI1787096, Length=547, Percent_Identity=23.0347349177331, Blast_Score=119, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI17542570, Length=502, Percent_Identity=25.6972111553785, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17531301, Length=457, Percent_Identity=24.945295404814, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17531299, Length=457, Percent_Identity=24.945295404814, Blast_Score=85, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6323755, Length=553, Percent_Identity=30.7414104882459, Blast_Score=233, Evalue=9e-62,
Organism=Saccharomyces cerevisiae, GI6320816, Length=253, Percent_Identity=27.2727272727273, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19922626, Length=448, Percent_Identity=26.5625, Blast_Score=129, Evalue=5e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 61721; Mature: 61721

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHLNDALAQQPDIAAVHCHHEQACGIAAEAYGRLAERTGVAMVTTGPGATNILTPVAGAW
CCCCHHHHCCCCEEEEEECCHHHCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHH
IESLPLLVISGQVKRADLLQGSGLRQKGVQEVDVVSMAKPVTKYAVTVTDPQAIRQHLEE
HHHCCEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCHHHHHHHHHH
ALYLAHSGRPGPVWLDIPLDVQAAPIDPATLPGWQPEGETAPAAQDHVAAIADLLKQAER
HHHHHCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PLILAGHGVRIAGGADALRRLSETLQVPVAATWNAMDLMPWDSPLCAGRPGVVALRPGNF
CEEEECCCEEEECCHHHHHHHHHHHCCCEEECCCCEECCCCCCCCCCCCCCEEEECCCCE
AVQNCDLLIAIGTRLDNILTAYNPKGFARAARKVVVDVDAAEIAKLEMEIDLPLAMDAKA
EEECCCEEEEECCHHHHHHHHCCCCHHHHHHCEEEEECCHHHHEEEEEEECCCCCCCHHH
CLEGLLAAVERLPKAKWQSWLDRCADWKRRYPVQDGAPFPTSGQISHYHFVDALSDAAPE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
NTLISTGSSGLAVEVFYTVFRNKPGQRVFLTSGLGSMGYGLPAAIGACLGNGSRPMIALE
CCEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEE
SDGSLQLNLQELATLKALRLPIRLFIMNNNGYASIRNTQRNYFDSRYVGTGPEAGVLIPD
CCCCEEEEHHHHHHHHHHCCCEEEEEECCCCEEEECCCHHHHCCCCCCCCCCCCCCCHHH
LLELAEAYGLPAVRIERVEELAEGLAKVMAHPGPILCDVRLNENETLQPKAAALPQPDGS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHCCCCCCCC
MLSMPLEDMSPLLPLEQLRAEMLIPLLPASEAAKRGV
EEECCHHHCCCCCCHHHHHHHHHCCCCCCCHHHHCCC
>Mature Secondary Structure
MHLNDALAQQPDIAAVHCHHEQACGIAAEAYGRLAERTGVAMVTTGPGATNILTPVAGAW
CCCCHHHHCCCCEEEEEECCHHHCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHH
IESLPLLVISGQVKRADLLQGSGLRQKGVQEVDVVSMAKPVTKYAVTVTDPQAIRQHLEE
HHHCCEEEEECCCCHHHHHCCCCCCCCCCCHHHHHHHHCCCEEEEEEECCHHHHHHHHHH
ALYLAHSGRPGPVWLDIPLDVQAAPIDPATLPGWQPEGETAPAAQDHVAAIADLLKQAER
HHHHHCCCCCCCEEEEECCCCEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
PLILAGHGVRIAGGADALRRLSETLQVPVAATWNAMDLMPWDSPLCAGRPGVVALRPGNF
CEEEECCCEEEECCHHHHHHHHHHHCCCEEECCCCEECCCCCCCCCCCCCCEEEECCCCE
AVQNCDLLIAIGTRLDNILTAYNPKGFARAARKVVVDVDAAEIAKLEMEIDLPLAMDAKA
EEECCCEEEEECCHHHHHHHHCCCCHHHHHHCEEEEECCHHHHEEEEEEECCCCCCCHHH
CLEGLLAAVERLPKAKWQSWLDRCADWKRRYPVQDGAPFPTSGQISHYHFVDALSDAAPE
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCC
NTLISTGSSGLAVEVFYTVFRNKPGQRVFLTSGLGSMGYGLPAAIGACLGNGSRPMIALE
CCEEECCCCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCHHHHHHHHHCCCCCCEEEEE
SDGSLQLNLQELATLKALRLPIRLFIMNNNGYASIRNTQRNYFDSRYVGTGPEAGVLIPD
CCCCEEEEHHHHHHHHHHCCCEEEEEECCCCEEEECCCHHHHCCCCCCCCCCCCCCCHHH
LLELAEAYGLPAVRIERVEELAEGLAKVMAHPGPILCDVRLNENETLQPKAAALPQPDGS
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHCCCCCCCC
MLSMPLEDMSPLLPLEQLRAEMLIPLLPASEAAKRGV
EEECCHHHCCCCCCHHHHHHHHHCCCCCCCHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377; 1577690; 9298659 [H]