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The map label for this gene is purB [H]

Identifier: 34499289

GI number: 34499289

Start: 4132018

End: 4133388

Strand: Direct

Name: purB [H]

Synonym: CV_3834

Alternate gene names: 34499289

Gene position: 4132018-4133388 (Clockwise)

Preceding gene: 34499288

Following gene: 34499290

Centisome position: 86.97

GC content: 63.53

Gene sequence:

>1371_bases
ATGAATCTGACTACGTTGACCGCCCTCTCTCCGCTGGATGGCCGCTACGCCGGCCAGGTGGAAGGCCTGCGCAACCTGTT
CTCCGAATTCGGCCTGATGAAGTGCCGCGTCAAGGTGGAGCTGGAATGGCTGAAAATGCTGGCCGCCGAGCCGGGCATCG
AAGAAGTCAAGCCGTTCTCCGACGCCACCATCCGCGAGATCGACGACGTGATCGCCAATTTCAGCGTCGAGCACGGCGAA
GAGGTGAAGGCGATCGAAGTCCGCACCAACCACGACGTCAAGGCGATCGAGTACTGGCTGAAGGAGCGCCTGTCGGGCAA
TCCGGAAGTGATGGCCGCCAGCGAGTTCATCCACTTCGCCTGCACCTCCGAGGACATCAACAACCTCAGCCACGCGCTGA
TGCTGAAGACCGCGCGCAACACCGTGCTGCTGCCGGCGCTGGACGAAGTGATCCACAAGCTGCAGAAGCTGGCGCACGAG
TTGGCCGACACGCCGATGATGTGCCGCACCCACGGCCAGCCGGCCACGCCGTCGACGATGGGCAAGGAGCTGGCCAACGC
CGTCTACCGCCTGAAGCGCCAGCGCGAGCAGCTGGTGCACCAGGAAATCCTGGGCAAGATTAACGGCGCGGTCGGCAACT
ACAACGCCCACCTGTCCGCCTACCCGGACATCGACTGGGAAAGCCTGTGCGGCCGCTTCGTCACCGGTCTGGGCATCACC
TTCAATCCGTACACCATCCAGATCGAGCCGCACGACTACATGGCCGAGCTGTACCAGGTGGCGATGCGCGTCAACACCAT
CCTGATCGACCTGAACCGCGACATCTGGGGCTACATCTCGCTGGGCTACTTCAAGCAGAAGGTGAAGAAGGACGAAGTGG
GCAGCTCCACCATGCCGCACAAGGTCAACCCGATCGACTTCGAAAACTCAGAAGGCAACCTGGGCATGGCCAACGCCATC
ATGGGCCACCTGGCGGAAAAACTGCCGATCTCCCGCTGGCAGCGCGATCTGACCGACTCCACCGTGCTGCGCAATATGGG
GGTCGGCTTCGGCTACACCATCCTGGGCCTGAAGTCCTGCCTGAAAGGCCTGAACAAGCTGGAGATCAATCCGGCCCTGA
TCGCGGCCGAGCTGGACGCCGCCTGGGAGCTGCTGGCCGAGCCGGTGCAGACCGTGATGCGCCGCTACGGCATCGCCAAC
CCGTACGAGCAGCTGAAGGAGCTGACCCGCGGCAAGGGCGGCATCACCCGCGAGACGCTGCACGCCTTCATCCGCGGCCT
GGAGCTGCCGGACGCGGTCAAGGCCAGCCTGCTGGAACTGACCCCGTCCAACTACTTGGGCAAGGCCGAGGAACTGGCGC
GCCGCATCTGA

Upstream 100 bases:

>100_bases
ACATTGATAGGAAGGCGAGCCGGCGCCCGATCGCGAATTTGAACGCCGTTTGTATTGCAACCTGCCGCTGTTTGGCAAAC
CACGTTTGGAAATACCCCGC

Downstream 100 bases:

>100_bases
CGCCATCCCCGCAGCCTCCCGCCGCGCCGCCCTCCCGGGCGGCGTTTTTCATGTCCCGCTCCGCCGGCCTCCCCGCCATT
TGGCAAGTTAGCCGCACCTC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MNLTTLTALSPLDGRYAGQVEGLRNLFSEFGLMKCRVKVELEWLKMLAAEPGIEEVKPFSDATIREIDDVIANFSVEHGE
EVKAIEVRTNHDVKAIEYWLKERLSGNPEVMAASEFIHFACTSEDINNLSHALMLKTARNTVLLPALDEVIHKLQKLAHE
LADTPMMCRTHGQPATPSTMGKELANAVYRLKRQREQLVHQEILGKINGAVGNYNAHLSAYPDIDWESLCGRFVTGLGIT
FNPYTIQIEPHDYMAELYQVAMRVNTILIDLNRDIWGYISLGYFKQKVKKDEVGSSTMPHKVNPIDFENSEGNLGMANAI
MGHLAEKLPISRWQRDLTDSTVLRNMGVGFGYTILGLKSCLKGLNKLEINPALIAAELDAAWELLAEPVQTVMRRYGIAN
PYEQLKELTRGKGGITRETLHAFIRGLELPDAVKASLLELTPSNYLGKAEELARRI

Sequences:

>Translated_456_residues
MNLTTLTALSPLDGRYAGQVEGLRNLFSEFGLMKCRVKVELEWLKMLAAEPGIEEVKPFSDATIREIDDVIANFSVEHGE
EVKAIEVRTNHDVKAIEYWLKERLSGNPEVMAASEFIHFACTSEDINNLSHALMLKTARNTVLLPALDEVIHKLQKLAHE
LADTPMMCRTHGQPATPSTMGKELANAVYRLKRQREQLVHQEILGKINGAVGNYNAHLSAYPDIDWESLCGRFVTGLGIT
FNPYTIQIEPHDYMAELYQVAMRVNTILIDLNRDIWGYISLGYFKQKVKKDEVGSSTMPHKVNPIDFENSEGNLGMANAI
MGHLAEKLPISRWQRDLTDSTVLRNMGVGFGYTILGLKSCLKGLNKLEINPALIAAELDAAWELLAEPVQTVMRRYGIAN
PYEQLKELTRGKGGITRETLHAFIRGLELPDAVKASLLELTPSNYLGKAEELARRI
>Mature_456_residues
MNLTTLTALSPLDGRYAGQVEGLRNLFSEFGLMKCRVKVELEWLKMLAAEPGIEEVKPFSDATIREIDDVIANFSVEHGE
EVKAIEVRTNHDVKAIEYWLKERLSGNPEVMAASEFIHFACTSEDINNLSHALMLKTARNTVLLPALDEVIHKLQKLAHE
LADTPMMCRTHGQPATPSTMGKELANAVYRLKRQREQLVHQEILGKINGAVGNYNAHLSAYPDIDWESLCGRFVTGLGIT
FNPYTIQIEPHDYMAELYQVAMRVNTILIDLNRDIWGYISLGYFKQKVKKDEVGSSTMPHKVNPIDFENSEGNLGMANAI
MGHLAEKLPISRWQRDLTDSTVLRNMGVGFGYTILGLKSCLKGLNKLEINPALIAAELDAAWELLAEPVQTVMRRYGIAN
PYEQLKELTRGKGGITRETLHAFIRGLELPDAVKASLLELTPSNYLGKAEELARRI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=449, Percent_Identity=61.4699331848552, Blast_Score=591, Evalue=1e-170,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51070; Mature: 51070

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTTLTALSPLDGRYAGQVEGLRNLFSEFGLMKCRVKVELEWLKMLAAEPGIEEVKPFS
CCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHCCCCHHHHCCCC
DATIREIDDVIANFSVEHGEEVKAIEVRTNHDVKAIEYWLKERLSGNPEVMAASEFIHFA
CHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEHHHHHEEE
CTSEDINNLSHALMLKTARNTVLLPALDEVIHKLQKLAHELADTPMMCRTHGQPATPSTM
ECHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHH
GKELANAVYRLKRQREQLVHQEILGKINGAVGNYNAHLSAYPDIDWESLCGRFVTGLGIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCE
FNPYTIQIEPHDYMAELYQVAMRVNTILIDLNRDIWGYISLGYFKQKVKKDEVGSSTMPH
ECCEEEEECCHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGMANAIMGHLAEKLPISRWQRDLTDSTVLRNMGVGFGYTILGLKSC
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LKGLNKLEINPALIAAELDAAWELLAEPVQTVMRRYGIANPYEQLKELTRGKGGITRETL
HHCHHHCCCCHHHEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHH
HAFIRGLELPDAVKASLLELTPSNYLGKAEELARRI
HHHHHCCCCCHHHHHHHHHCCCCHHCCCHHHHHHCC
>Mature Secondary Structure
MNLTTLTALSPLDGRYAGQVEGLRNLFSEFGLMKCRVKVELEWLKMLAAEPGIEEVKPFS
CCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEHHHHHHHHHCCCCHHHHCCCC
DATIREIDDVIANFSVEHGEEVKAIEVRTNHDVKAIEYWLKERLSGNPEVMAASEFIHFA
CHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHCCCCCEEEHHHHHEEE
CTSEDINNLSHALMLKTARNTVLLPALDEVIHKLQKLAHELADTPMMCRTHGQPATPSTM
ECHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHH
GKELANAVYRLKRQREQLVHQEILGKINGAVGNYNAHLSAYPDIDWESLCGRFVTGLGIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEECCCCCCHHHHHHHHHHHCCCE
FNPYTIQIEPHDYMAELYQVAMRVNTILIDLNRDIWGYISLGYFKQKVKKDEVGSSTMPH
ECCEEEEECCHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCC
KVNPIDFENSEGNLGMANAIMGHLAEKLPISRWQRDLTDSTVLRNMGVGFGYTILGLKSC
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
LKGLNKLEINPALIAAELDAAWELLAEPVQTVMRRYGIANPYEQLKELTRGKGGITRETL
HHCHHHCCCCHHHEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHH
HAFIRGLELPDAVKASLLELTPSNYLGKAEELARRI
HHHHHCCCCCHHHHHHHHHCCCCHHCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]