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The map label for this gene is pilB [H]

Identifier: 34499283

GI number: 34499283

Start: 4125777

End: 4127483

Strand: Reverse

Name: pilB [H]

Synonym: CV_3828

Alternate gene names: 34499283

Gene position: 4127483-4125777 (Counterclockwise)

Preceding gene: 34499284

Following gene: 34499282

Centisome position: 86.87

GC content: 60.28

Gene sequence:

>1707_bases
ATGGAAGCTACGGCAGGAATTTCCGGCCTGGGCAGAGCCCTGGTTCAGCACAATATGCTATTGCAGCAAGATGCGGATGC
CATCTCAAAACAGGCGACGACGTCGCATCTCAGCTTCGTCGAGCAACTGATCCAGAGCAAGAAGATGACGTCGCGCGATA
TCGCGATGTTTTCATCGCGGGTTTTCGGCTATCCGCTGCTGGATTTGGCTTCGCTGGACACCGAACAATTGCCCAAAGGG
TTGGTGGACGACGAGGTGATCGCCAGCCGCCGCCTTTTGCCGTTGCTGAAGCGCGGCAACAAGCTGTATATCGGCACTTC
CGATCCTACCCAGACCTCGGCCTATCATGCGATCACCTTCAAGACCGGCTTGACGGTCGAGGTGGTGGTGGTGGAAGAGG
ACAAGCTGTCCAAGCTGATCGGCCGTTACCAGGACAATTCGACCTCGGCTTTGAAGGAGCTGGAGAGCGAGGACTTCGGC
GACCTGGAGTTCGCCGATCCGGACGCCCAGCTCGACAGCGGCCCGCAGACCGAGGTCGACGACGCGCCGGTGGTCAAGTT
CATTCACAAGATGCTGCTGGACGCGATAGGCATCGGCGCGTCCGACATCCACTTCGAACCTTACGAGAAATATTACCGCA
TCCGCTACCGAGTGGACGGCGTGCTGAAGGAGGTGGCGCAGCCGCCCTTGCTGCTGAAGGAAAAGATCGCCTCCCGCATC
AAGGTGATTTCCAGGCTGGACATTTCGGAAAAGCGCTTGCCGCAGGATGGCCGCTTGAAGCTGGTGATTTCCAAGACCCG
GGCGATAGATTTCCGCGTCAGCACGCTGCCCACGCTGTACGGCGAGAAGATCGTCATGCGTATCCTGGACTCGTCCGGCG
CCTCGCTGGATATCGACCAGCTGGGTTTCGAGCCGGAGCAGAAGGCGCATCTGCTGAAGGCGATCGCCAGGCCGTACGGC
ATGGTGCTGGTGACCGGTCCGACGGGCAGCGGCAAGACTGTGTCGCTGTACACCTGCCTGAACATCCTGAACAAGCCGGA
CACCAATATCTCCACGGCGGAGGATCCGGTTGAAATCAACCTTCCCGGCATCAACCAAGTCAACGTCAACGAGAAGGCCG
GGCTGACCTTCGCGTCGGCGCTGAAAGCCTTCTTGCGGCAGGATCCGGACGTGATCATGGTCGGCGAAATCCGCGATTTC
GAAACCGCCGACATCGCCATCAAGGCGGCTCAGACCGGCCACATGGTGTTTTCCACGCTGCATACCAATGACGCCCCGTC
CACGCTGACCCGGATGCTGAATATGGGCGTGGCGCCGTTCAACATCGCCAGTTCGGTGTTGCTGATCATGGCGCAGCGCC
TGGCGCGCCGCTTGTGCAGCCATTGCAAGCAGCCGGTGGAGATCCCGAAGGAAGCGCTGCTGCGCGCGGGTTTTACCAAA
GAGGACCTGGCGGGCGGCTGGCATCCGTACGGGCCGGTTGGCTGCGAGGAGTGCCGGAATACCGGCTACAAAGGCCGGGT
CGGCATTTTCGAAGCCATGCCGATCAGCGACGCGATGATGCGCTTGATCATGAAGAACGGCACCTCGGTCGACATCTCCG
ATCTGGCCAAGGAGGAGGGCATGGTCGATCTGCGCCGCGCCGGCCTGTTGAAGGTGATGAGCGGAGTGACTTCGCTGGCC
GAGGTCGAAGCGGTAACCAACGATTGA

Upstream 100 bases:

>100_bases
GCCTTGATAGACAGGGATTTGCCAGCCCGATTCGCATATAATACCGTGGCAGCCTGTACCTGATCACGACCGCAAGGTCC
GAACTGGATCGATTTCGCTT

Downstream 100 bases:

>100_bases
ACGGCCCCGCCGGCAAAGCTCCGCCTCCGCCCTGGGCCAAGGCGCGCATGTGCCGCGCGGCAAGAGCGCGGCGCCGGCAG
GATCAGGGGTTTTGTGGGCG

Product: type 4 fimbrial biogenesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 568; Mature: 568

Protein sequence:

>568_residues
MEATAGISGLGRALVQHNMLLQQDADAISKQATTSHLSFVEQLIQSKKMTSRDIAMFSSRVFGYPLLDLASLDTEQLPKG
LVDDEVIASRRLLPLLKRGNKLYIGTSDPTQTSAYHAITFKTGLTVEVVVVEEDKLSKLIGRYQDNSTSALKELESEDFG
DLEFADPDAQLDSGPQTEVDDAPVVKFIHKMLLDAIGIGASDIHFEPYEKYYRIRYRVDGVLKEVAQPPLLLKEKIASRI
KVISRLDISEKRLPQDGRLKLVISKTRAIDFRVSTLPTLYGEKIVMRILDSSGASLDIDQLGFEPEQKAHLLKAIARPYG
MVLVTGPTGSGKTVSLYTCLNILNKPDTNISTAEDPVEINLPGINQVNVNEKAGLTFASALKAFLRQDPDVIMVGEIRDF
ETADIAIKAAQTGHMVFSTLHTNDAPSTLTRMLNMGVAPFNIASSVLLIMAQRLARRLCSHCKQPVEIPKEALLRAGFTK
EDLAGGWHPYGPVGCEECRNTGYKGRVGIFEAMPISDAMMRLIMKNGTSVDISDLAKEEGMVDLRRAGLLKVMSGVTSLA
EVEAVTND

Sequences:

>Translated_568_residues
MEATAGISGLGRALVQHNMLLQQDADAISKQATTSHLSFVEQLIQSKKMTSRDIAMFSSRVFGYPLLDLASLDTEQLPKG
LVDDEVIASRRLLPLLKRGNKLYIGTSDPTQTSAYHAITFKTGLTVEVVVVEEDKLSKLIGRYQDNSTSALKELESEDFG
DLEFADPDAQLDSGPQTEVDDAPVVKFIHKMLLDAIGIGASDIHFEPYEKYYRIRYRVDGVLKEVAQPPLLLKEKIASRI
KVISRLDISEKRLPQDGRLKLVISKTRAIDFRVSTLPTLYGEKIVMRILDSSGASLDIDQLGFEPEQKAHLLKAIARPYG
MVLVTGPTGSGKTVSLYTCLNILNKPDTNISTAEDPVEINLPGINQVNVNEKAGLTFASALKAFLRQDPDVIMVGEIRDF
ETADIAIKAAQTGHMVFSTLHTNDAPSTLTRMLNMGVAPFNIASSVLLIMAQRLARRLCSHCKQPVEIPKEALLRAGFTK
EDLAGGWHPYGPVGCEECRNTGYKGRVGIFEAMPISDAMMRLIMKNGTSVDISDLAKEEGMVDLRRAGLLKVMSGVTSLA
EVEAVTND
>Mature_568_residues
MEATAGISGLGRALVQHNMLLQQDADAISKQATTSHLSFVEQLIQSKKMTSRDIAMFSSRVFGYPLLDLASLDTEQLPKG
LVDDEVIASRRLLPLLKRGNKLYIGTSDPTQTSAYHAITFKTGLTVEVVVVEEDKLSKLIGRYQDNSTSALKELESEDFG
DLEFADPDAQLDSGPQTEVDDAPVVKFIHKMLLDAIGIGASDIHFEPYEKYYRIRYRVDGVLKEVAQPPLLLKEKIASRI
KVISRLDISEKRLPQDGRLKLVISKTRAIDFRVSTLPTLYGEKIVMRILDSSGASLDIDQLGFEPEQKAHLLKAIARPYG
MVLVTGPTGSGKTVSLYTCLNILNKPDTNISTAEDPVEINLPGINQVNVNEKAGLTFASALKAFLRQDPDVIMVGEIRDF
ETADIAIKAAQTGHMVFSTLHTNDAPSTLTRMLNMGVAPFNIASSVLLIMAQRLARRLCSHCKQPVEIPKEALLRAGFTK
EDLAGGWHPYGPVGCEECRNTGYKGRVGIFEAMPISDAMMRLIMKNGTSVDISDLAKEEGMVDLRRAGLLKVMSGVTSLA
EVEAVTND

Specific function: Involved in the translocation of the type IV pilin (pilA) [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=389, Percent_Identity=48.3290488431877, Blast_Score=346, Evalue=3e-96,
Organism=Escherichia coli, GI1786296, Length=389, Percent_Identity=44.4730077120823, Blast_Score=328, Evalue=4e-91,
Organism=Escherichia coli, GI87082188, Length=239, Percent_Identity=35.1464435146443, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013374
- InterPro:   IPR007831
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E; PF05157 GSPII_E_N [H]

EC number: NA

Molecular weight: Translated: 62277; Mature: 62277

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEATAGISGLGRALVQHNMLLQQDADAISKQATTSHLSFVEQLIQSKKMTSRDIAMFSSR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VFGYPLLDLASLDTEQLPKGLVDDEVIASRRLLPLLKRGNKLYIGTSDPTQTSAYHAITF
HCCCCHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEE
KTGLTVEVVVVEEDKLSKLIGRYQDNSTSALKELESEDFGDLEFADPDAQLDSGPQTEVD
ECCCEEEEEEECCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
DAPVVKFIHKMLLDAIGIGASDIHFEPYEKYYRIRYRVDGVLKEVAQPPLLLKEKIASRI
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
KVISRLDISEKRLPQDGRLKLVISKTRAIDFRVSTLPTLYGEKIVMRILDSSGASLDIDQ
HHHHHCCCHHHHCCCCCCEEEEEEECCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEHHH
LGFEPEQKAHLLKAIARPYGMVLVTGPTGSGKTVSLYTCLNILNKPDTNISTAEDPVEIN
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEHHHHHHHHCCCCCCCCCCCCCEEEE
LPGINQVNVNEKAGLTFASALKAFLRQDPDVIMVGEIRDFETADIAIKAAQTGHMVFSTL
CCCCCEECCCCHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCEEEEEE
HTNDAPSTLTRMLNMGVAPFNIASSVLLIMAQRLARRLCSHCKQPVEIPKEALLRAGFTK
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCH
EDLAGGWHPYGPVGCEECRNTGYKGRVGIFEAMPISDAMMRLIMKNGTSVDISDLAKEEG
HHHCCCCCCCCCCCHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCHHHHHHHCC
MVDLRRAGLLKVMSGVTSLAEVEAVTND
CHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEATAGISGLGRALVQHNMLLQQDADAISKQATTSHLSFVEQLIQSKKMTSRDIAMFSSR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
VFGYPLLDLASLDTEQLPKGLVDDEVIASRRLLPLLKRGNKLYIGTSDPTQTSAYHAITF
HCCCCHHHHHCCCHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEE
KTGLTVEVVVVEEDKLSKLIGRYQDNSTSALKELESEDFGDLEFADPDAQLDSGPQTEVD
ECCCEEEEEEECCHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCC
DAPVVKFIHKMLLDAIGIGASDIHFEPYEKYYRIRYRVDGVLKEVAQPPLLLKEKIASRI
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
KVISRLDISEKRLPQDGRLKLVISKTRAIDFRVSTLPTLYGEKIVMRILDSSGASLDIDQ
HHHHHCCCHHHHCCCCCCEEEEEEECCEEEEEHHHHHHHHHHHHHHHHHCCCCCEEEHHH
LGFEPEQKAHLLKAIARPYGMVLVTGPTGSGKTVSLYTCLNILNKPDTNISTAEDPVEIN
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCEEEHHHHHHHHCCCCCCCCCCCCCEEEE
LPGINQVNVNEKAGLTFASALKAFLRQDPDVIMVGEIRDFETADIAIKAAQTGHMVFSTL
CCCCCEECCCCHHCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEECCCCCEEEEEE
HTNDAPSTLTRMLNMGVAPFNIASSVLLIMAQRLARRLCSHCKQPVEIPKEALLRAGFTK
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCCH
EDLAGGWHPYGPVGCEECRNTGYKGRVGIFEAMPISDAMMRLIMKNGTSVDISDLAKEEG
HHHCCCCCCCCCCCHHHHHCCCCCCCCCEEECCCHHHHHHHHHHHCCCCCCHHHHHHHCC
MVDLRRAGLLKVMSGVTSLAEVEAVTND
CHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1971619; 10984043 [H]