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The map label for this gene is carA [H]

Identifier: 34499259

GI number: 34499259

Start: 4102380

End: 4103507

Strand: Reverse

Name: carA [H]

Synonym: CV_3804

Alternate gene names: 34499259

Gene position: 4103507-4102380 (Counterclockwise)

Preceding gene: 34499260

Following gene: 34499258

Centisome position: 86.37

GC content: 65.34

Gene sequence:

>1128_bases
GTGTCAAACCTCCCCGCAATCCTTGTCTTGGCTGACGGCACGCTTTTCAAAGGTAGCGCTATCGGTGCCGCCGGCCATAC
GGTCGGCGAGGTGGTATTCAATACCGCCATCACCGGTTACCAGGAGATCCTGACGGATCCCTCCTATACCAGGCAGATCG
TCACACTCACTTATCCGCACATCGGCAATGTCGGCGCGAATTCGGAAGACACCGAATCCCGCGCCGTTTTTGCATCCGGC
CTGATCATCCGCGATCTGCCGCTGCTGCACAGCAACTTCCGCGCCGAAGATTCGCTGTCCGATTACCTGAAAAAGCACAA
TGTGGTGGCGATCGCCGACATCGACACCCGCAAGCTGACCCGCATCCTGCGCGAGAAGGGCGCCCAGGCCGGCTGCATCA
TGGCCGGCGACATCGACGAGGCGAAGGCGCTGGAGCTGGCGCGCGGCTTCGGCAGCATGGCCGGCCAGGACCTGGCCAAG
GTCGTCAGCTGCCAGAAGCCGTACGCCTGGACCGAGAAGCAATGGAAGCTGGGCGCCGGTTACCAGGTCCAGGCCTCCAC
CCGCTTCCACGTGGTGGCCTACGACTTCGGCGTCAAGCACAACATCCTGCGCATGCTGGCCGAACGCGGCTGCCAGCTGA
CCATCGTCCCGGCCCAGACGCCGGCGGCCGATGTGCTGGCGCTGAATCCGCACGGCGTGTTCCTGTCCAACGGCCCCGGC
GATCCGGAGCCGTGCGGCTACGCGATTGCCGCGATCCGCGACATCCTGGAGACCAAGCTGCCGGTGTTCGGCATCTGCCT
GGGCCACCAGTTGCTGGGCCTGGCCACCGGCGCCGCCACTTCCAAGATGAAGTTCGGCCACCACGGCGCCAACCACCCGG
TGCAGGACCTGGATACCGGCCGCGTGATGATCACCAGCCAGAACCACGGCTTCCAGGTGGATGAGACCAGCTTGCCGGCC
AATGTCCGCGTCACCCACCGCTCGCTGTTCGACGGCACGGTACAGGGCATCGCGCTGACCGACCGCCCGGCGTTCTCGTT
CCAGGGCCACCCGGAAGCGAGCCCGGGGCCGGAGGACGTCGCCTACCTGTTCGACCGCTTCATCGCGGCGATGGCGGAAC
GGCAGTAA

Upstream 100 bases:

>100_bases
GTTATTCACATAAGAATTTTGAAAATCCCAGGGTGTAAAACGAAACCGGCCGCTGCCGGTTTTTGTGTAAGCGCCCGAGC
AATGCAACAGGAGCCAGCCA

Downstream 100 bases:

>100_bases
GGCGGGATAGCAATATGTTGGGCATTACCGACCTTACCACCTACCTGATCGGCACGCTGATCGTGATCCTGCTGCCGGGC
CCGAACGCCATGTACGTGTT

Product: carbamoyl phosphate synthase small subunit

Products: NA

Alternate protein names: Carbamoyl-phosphate synthetase glutamine chain [H]

Number of amino acids: Translated: 375; Mature: 374

Protein sequence:

>375_residues
MSNLPAILVLADGTLFKGSAIGAAGHTVGEVVFNTAITGYQEILTDPSYTRQIVTLTYPHIGNVGANSEDTESRAVFASG
LIIRDLPLLHSNFRAEDSLSDYLKKHNVVAIADIDTRKLTRILREKGAQAGCIMAGDIDEAKALELARGFGSMAGQDLAK
VVSCQKPYAWTEKQWKLGAGYQVQASTRFHVVAYDFGVKHNILRMLAERGCQLTIVPAQTPAADVLALNPHGVFLSNGPG
DPEPCGYAIAAIRDILETKLPVFGICLGHQLLGLATGAATSKMKFGHHGANHPVQDLDTGRVMITSQNHGFQVDETSLPA
NVRVTHRSLFDGTVQGIALTDRPAFSFQGHPEASPGPEDVAYLFDRFIAAMAERQ

Sequences:

>Translated_375_residues
MSNLPAILVLADGTLFKGSAIGAAGHTVGEVVFNTAITGYQEILTDPSYTRQIVTLTYPHIGNVGANSEDTESRAVFASG
LIIRDLPLLHSNFRAEDSLSDYLKKHNVVAIADIDTRKLTRILREKGAQAGCIMAGDIDEAKALELARGFGSMAGQDLAK
VVSCQKPYAWTEKQWKLGAGYQVQASTRFHVVAYDFGVKHNILRMLAERGCQLTIVPAQTPAADVLALNPHGVFLSNGPG
DPEPCGYAIAAIRDILETKLPVFGICLGHQLLGLATGAATSKMKFGHHGANHPVQDLDTGRVMITSQNHGFQVDETSLPA
NVRVTHRSLFDGTVQGIALTDRPAFSFQGHPEASPGPEDVAYLFDRFIAAMAERQ
>Mature_374_residues
SNLPAILVLADGTLFKGSAIGAAGHTVGEVVFNTAITGYQEILTDPSYTRQIVTLTYPHIGNVGANSEDTESRAVFASGL
IIRDLPLLHSNFRAEDSLSDYLKKHNVVAIADIDTRKLTRILREKGAQAGCIMAGDIDEAKALELARGFGSMAGQDLAKV
VSCQKPYAWTEKQWKLGAGYQVQASTRFHVVAYDFGVKHNILRMLAERGCQLTIVPAQTPAADVLALNPHGVFLSNGPGD
PEPCGYAIAAIRDILETKLPVFGICLGHQLLGLATGAATSKMKFGHHGANHPVQDLDTGRVMITSQNHGFQVDETSLPAN
VRVTHRSLFDGTVQGIALTDRPAFSFQGHPEASPGPEDVAYLFDRFIAAMAERQ

Specific function: Arginine biosynthesis. Pyrimidine biosynthesis; first step. [C]

COG id: COG0505

COG function: function code EF; Carbamoylphosphate synthase small subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI18105007, Length=379, Percent_Identity=37.2031662269129, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI21361331, Length=379, Percent_Identity=36.1477572559367, Blast_Score=215, Evalue=6e-56,
Organism=Homo sapiens, GI169790915, Length=379, Percent_Identity=36.1477572559367, Blast_Score=215, Evalue=6e-56,
Organism=Escherichia coli, GI1786215, Length=367, Percent_Identity=69.7547683923706, Blast_Score=528, Evalue=1e-151,
Organism=Caenorhabditis elegans, GI193204318, Length=377, Percent_Identity=38.1962864721485, Blast_Score=244, Evalue=4e-65,
Organism=Saccharomyces cerevisiae, GI6324878, Length=385, Percent_Identity=37.1428571428571, Blast_Score=239, Evalue=5e-64,
Organism=Saccharomyces cerevisiae, GI6322331, Length=392, Percent_Identity=34.9489795918367, Blast_Score=223, Evalue=4e-59,
Organism=Drosophila melanogaster, GI45555749, Length=395, Percent_Identity=36.4556962025316, Blast_Score=224, Evalue=1e-58,
Organism=Drosophila melanogaster, GI24642586, Length=395, Percent_Identity=36.4556962025316, Blast_Score=223, Evalue=1e-58,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2599 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006220
- InterPro:   IPR001317
- InterPro:   IPR006274
- InterPro:   IPR002474
- InterPro:   IPR011702
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF00988 CPSase_sm_chain; PF00117 GATase [H]

EC number: =6.3.5.5 [H]

Molecular weight: Translated: 40300; Mature: 40169

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNLPAILVLADGTLFKGSAIGAAGHTVGEVVFNTAITGYQEILTDPSYTRQIVTLTYPH
CCCCCEEEEEECCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCC
IGNVGANSEDTESRAVFASGLIIRDLPLLHSNFRAEDSLSDYLKKHNVVAIADIDTRKLT
CCCCCCCCCCCHHHHHHHCCCHHHCCHHHHCCCCCCHHHHHHHHHCCEEEEEECCHHHHH
RILREKGAQAGCIMAGDIDEAKALELARGFGSMAGQDLAKVVSCQKPYAWTEKQWKLGAG
HHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCC
YQVQASTRFHVVAYDFGVKHNILRMLAERGCQLTIVPAQTPAADVLALNPHGVFLSNGPG
EEEEECCEEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCEEEEECCCC
DPEPCGYAIAAIRDILETKLPVFGICLGHQLLGLATGAATSKMKFGHHGANHPVQDLDTG
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCC
RVMITSQNHGFQVDETSLPANVRVTHRSLFDGTVQGIALTDRPAFSFQGHPEASPGPEDV
EEEEEECCCCEEECCCCCCCEEEEEEHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHH
AYLFDRFIAAMAERQ
HHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNLPAILVLADGTLFKGSAIGAAGHTVGEVVFNTAITGYQEILTDPSYTRQIVTLTYPH
CCCCEEEEEECCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEEECCC
IGNVGANSEDTESRAVFASGLIIRDLPLLHSNFRAEDSLSDYLKKHNVVAIADIDTRKLT
CCCCCCCCCCCHHHHHHHCCCHHHCCHHHHCCCCCCHHHHHHHHHCCEEEEEECCHHHHH
RILREKGAQAGCIMAGDIDEAKALELARGFGSMAGQDLAKVVSCQKPYAWTEKQWKLGAG
HHHHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEECCCC
YQVQASTRFHVVAYDFGVKHNILRMLAERGCQLTIVPAQTPAADVLALNPHGVFLSNGPG
EEEEECCEEEEEEEECCCHHHHHHHHHHCCCEEEEEECCCCCCCEEEECCCEEEEECCCC
DPEPCGYAIAAIRDILETKLPVFGICLGHQLLGLATGAATSKMKFGHHGANHPVQDLDTG
CCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCEECCCCCCCCCCCCCCCC
RVMITSQNHGFQVDETSLPANVRVTHRSLFDGTVQGIALTDRPAFSFQGHPEASPGPEDV
EEEEEECCCCEEECCCCCCCEEEEEEHHHHCCCCCEEEEECCCCCCCCCCCCCCCCHHHH
AYLFDRFIAAMAERQ
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11248100 [H]