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The map label for this gene is ftsJ

Identifier: 34499253

GI number: 34499253

Start: 4096178

End: 4096795

Strand: Reverse

Name: ftsJ

Synonym: CV_3798

Alternate gene names: 34499253

Gene position: 4096795-4096178 (Counterclockwise)

Preceding gene: 34499255

Following gene: 34499252

Centisome position: 86.23

GC content: 60.03

Gene sequence:

>618_bases
ATGGCAAGAAGCAAGAGCAGCAACAACTGGTTGCAGGAGCACGTCAACGATCAATACGTGCACATGGCGCAGAAGGACGG
TTACCGCGCCCGCGCCGCCTACAAACTGCTGGAAATCAACGACAAGGACAAGCTGATCCGGCCGGGCACCGTGCTGGCCG
ACCTGGGCTCGACGCCGGGCAGCTGGTCGCAGGTGGCGGCGCGCATCGTCGGGGAAAAGGGCAAGGTGTTCGCGCTGGAC
ATCCTGGACATGGATCCGGTGCCGGGCGTGGATTTTATCCAGGGCGACTTCCGCGAGGAAGCGGTGTTGCGCGAATTCGA
GCAATTATTGGATGGACGCGCGCTCGACCTTGTAATTTCCGACATGGCGCCCAATATGTCAGGCATGAGCGCCATCGATC
AGGCCAGGAGTTTCCTGCTGTGCGAGCTGGCGCTGGAATTTGCGCGCGATCATTTGAAACCTGGCGGGCACTTTCTCGTC
AAAGTGTTCCAGGGAAGCGATTTTCAACCCTACCTCAAGGCCATGCGCGAGCTGTTCGACGAGGTGGTGACGCGCAAGCC
CAAGGCATCGCGCGACCGTTCCAGTGAAATCTATCTGCTGGGCAAGGGTCGCCGCTGA

Upstream 100 bases:

>100_bases
AAAGTTTGGGGCTGAAATAACCGAAAAACAACAGGATAGCGCGCCGTCCGGCGCGCATCGGCTATCCTGACGAGTGAGAC
GACAGAAAGCACCGCCGCGT

Downstream 100 bases:

>100_bases
CATAAAAGGGCGCGCAGCGTACAATCGTAGCCATTCAAAACAAGCAACCAAAAAGTCAGGGCACAGAGGAGTCCGCCACT
CGTGAACAATATCGGCAAAA

Product: cell division protein FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MARSKSSNNWLQEHVNDQYVHMAQKDGYRARAAYKLLEINDKDKLIRPGTVLADLGSTPGSWSQVAARIVGEKGKVFALD
ILDMDPVPGVDFIQGDFREEAVLREFEQLLDGRALDLVISDMAPNMSGMSAIDQARSFLLCELALEFARDHLKPGGHFLV
KVFQGSDFQPYLKAMRELFDEVVTRKPKASRDRSSEIYLLGKGRR

Sequences:

>Translated_205_residues
MARSKSSNNWLQEHVNDQYVHMAQKDGYRARAAYKLLEINDKDKLIRPGTVLADLGSTPGSWSQVAARIVGEKGKVFALD
ILDMDPVPGVDFIQGDFREEAVLREFEQLLDGRALDLVISDMAPNMSGMSAIDQARSFLLCELALEFARDHLKPGGHFLV
KVFQGSDFQPYLKAMRELFDEVVTRKPKASRDRSSEIYLLGKGRR
>Mature_204_residues
ARSKSSNNWLQEHVNDQYVHMAQKDGYRARAAYKLLEINDKDKLIRPGTVLADLGSTPGSWSQVAARIVGEKGKVFALDI
LDMDPVPGVDFIQGDFREEAVLREFEQLLDGRALDLVISDMAPNMSGMSAIDQARSFLLCELALEFARDHLKPGGHFLVK
VFQGSDFQPYLKAMRELFDEVVTRKPKASRDRSSEIYLLGKGRR

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=208, Percent_Identity=36.5384615384615, Blast_Score=145, Evalue=2e-35,
Organism=Homo sapiens, GI29029591, Length=189, Percent_Identity=37.037037037037, Blast_Score=130, Evalue=6e-31,
Organism=Homo sapiens, GI29029589, Length=189, Percent_Identity=37.037037037037, Blast_Score=130, Evalue=6e-31,
Organism=Homo sapiens, GI7110661, Length=189, Percent_Identity=37.037037037037, Blast_Score=130, Evalue=7e-31,
Organism=Homo sapiens, GI194097365, Length=187, Percent_Identity=34.2245989304813, Blast_Score=126, Evalue=1e-29,
Organism=Escherichia coli, GI1789569, Length=203, Percent_Identity=52.2167487684729, Blast_Score=225, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17553474, Length=201, Percent_Identity=33.3333333333333, Blast_Score=120, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI17554650, Length=187, Percent_Identity=32.620320855615, Blast_Score=112, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI71987550, Length=184, Percent_Identity=30.4347826086957, Blast_Score=102, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI71987561, Length=184, Percent_Identity=30.4347826086957, Blast_Score=102, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17553860, Length=184, Percent_Identity=30.4347826086957, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6319796, Length=191, Percent_Identity=32.9842931937173, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319535, Length=197, Percent_Identity=33.502538071066, Blast_Score=113, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6321302, Length=109, Percent_Identity=36.697247706422, Blast_Score=71, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21356387, Length=209, Percent_Identity=37.3205741626794, Blast_Score=141, Evalue=2e-34,
Organism=Drosophila melanogaster, GI18859957, Length=187, Percent_Identity=31.0160427807487, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648639, Length=194, Percent_Identity=30.4123711340206, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24647580, Length=194, Percent_Identity=29.3814432989691, Blast_Score=89, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_CHRVO (Q7NRI3)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903468.1
- ProteinModelPortal:   Q7NRI3
- SMR:   Q7NRI3
- GeneID:   2547545
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3798
- NMPDR:   fig|243365.1.peg.3798
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- PhylomeDB:   Q7NRI3
- ProtClustDB:   CLSK877754
- BioCyc:   CVIO243365:CV_3798-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 23089; Mature: 22958

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: NA

Important sites: ACT_SITE 161-161 BINDING 60-60 BINDING 62-62 BINDING 80-80 BINDING 96-96 BINDING 121-121

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARSKSSNNWLQEHVNDQYVHMAQKDGYRARAAYKLLEINDKDKLIRPGTVLADLGSTPG
CCCCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHEEEECCCCCEECCCHHEEECCCCCC
SWSQVAARIVGEKGKVFALDILDMDPVPGVDFIQGDFREEAVLREFEQLLDGRALDLVIS
CHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCHHEEEHH
DMAPNMSGMSAIDQARSFLLCELALEFARDHLKPGGHFLVKVFQGSDFQPYLKAMRELFD
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHH
EVVTRKPKASRDRSSEIYLLGKGRR
HHHHCCCCCCCCCCCEEEEEECCCC
>Mature Secondary Structure 
ARSKSSNNWLQEHVNDQYVHMAQKDGYRARAAYKLLEINDKDKLIRPGTVLADLGSTPG
CCCCCCCHHHHHHCCHHHHHHHHCCCCHHHHHHHEEEECCCCCEECCCHHEEECCCCCC
SWSQVAARIVGEKGKVFALDILDMDPVPGVDFIQGDFREEAVLREFEQLLDGRALDLVIS
CHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHCCHHHHHHHHHHHHHHCCCHHEEEHH
DMAPNMSGMSAIDQARSFLLCELALEFARDHLKPGGHFLVKVFQGSDFQPYLKAMRELFD
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHH
EVVTRKPKASRDRSSEIYLLGKGRR
HHHHCCCCCCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782