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The map label for this gene is trmB

Identifier: 34499241

GI number: 34499241

Start: 4083148

End: 4083828

Strand: Direct

Name: trmB

Synonym: CV_3786

Alternate gene names: 34499241

Gene position: 4083148-4083828 (Clockwise)

Preceding gene: 34499240

Following gene: 34499243

Centisome position: 85.94

GC content: 63.58

Gene sequence:

>681_bases
ATGGAAAATCCGGCTTTCAAACGCGCGATCCGCAGCTTTGTGCTGCGCCAGGGCCATCTGTCCGCAGGCCAGCAGCGGGC
GATGGACGAAGGCATGCCCAAGTGGGGCATAGAGTACCGTCCGGAAACGATGGATCTGGAACAGGTCTTCGGCCGCGCCG
CGCCCAAGATCCTGGAAATCGGCTTCGGCATGGGCGGCGCCACCGCCGAGATCGCCGCCGCCAACCCGGACAATGACTAT
CTGGGCATCGAGGTGCACGGCCCCGGCGTCGGCAATCTGTGCAAGCTGATCGCGGAAAAAGAGCTGACCAATCTGCGGCT
GATGCGCCACGACGCGGTGGAAGTGCTGGACAATATGCTGGCCGACGGCAGCCTGGACGGCGTGCACATTTTCTTCCCGG
ATCCGTGGCACAAGAAACGCCACAACAAGCGCCGGCTGATCCAGGCGCCGCTGGTGGAAAAGCTGGCCAAGAAGCTGAAG
CCGGGCGGCTACTTCCACGCCGCCACCGACTGGGAAGACTACGCGATCCAGATCCTGGAAGTGCTGAACGGCAACGCCGA
TCTGGAAAACACCGCCGACGGCTACGCGCCGCGCCCGGACTACCGCCCGCTGACCAAGTTCGAAGCCCGCGGCATCAAGC
TGGGCCATGGCGTGTGGGATGTGATCTTCCGCCGCAAGTAA

Upstream 100 bases:

>100_bases
GCGCTGCGGCGGGACGAGGGCCGGCGGTCGGCAATACTCGCAAGCTACGCCGAGATTCGGTAACATCGCGGTTTTTTTGA
GTTTTCAGCAGGCCAGCAAA

Downstream 100 bases:

>100_bases
GCGATCCGCTCTGCAGCCACGGCCCGCTCGCGCGGGCCGTTTTTCTTTCACCTCGCCAGCAGCGCCTTGATGTCGTCTAC
CAGCTCGTGCGGCTCCACCG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MENPAFKRAIRSFVLRQGHLSAGQQRAMDEGMPKWGIEYRPETMDLEQVFGRAAPKILEIGFGMGGATAEIAAANPDNDY
LGIEVHGPGVGNLCKLIAEKELTNLRLMRHDAVEVLDNMLADGSLDGVHIFFPDPWHKKRHNKRRLIQAPLVEKLAKKLK
PGGYFHAATDWEDYAIQILEVLNGNADLENTADGYAPRPDYRPLTKFEARGIKLGHGVWDVIFRRK

Sequences:

>Translated_226_residues
MENPAFKRAIRSFVLRQGHLSAGQQRAMDEGMPKWGIEYRPETMDLEQVFGRAAPKILEIGFGMGGATAEIAAANPDNDY
LGIEVHGPGVGNLCKLIAEKELTNLRLMRHDAVEVLDNMLADGSLDGVHIFFPDPWHKKRHNKRRLIQAPLVEKLAKKLK
PGGYFHAATDWEDYAIQILEVLNGNADLENTADGYAPRPDYRPLTKFEARGIKLGHGVWDVIFRRK
>Mature_226_residues
MENPAFKRAIRSFVLRQGHLSAGQQRAMDEGMPKWGIEYRPETMDLEQVFGRAAPKILEIGFGMGGATAEIAAANPDNDY
LGIEVHGPGVGNLCKLIAEKELTNLRLMRHDAVEVLDNMLADGSLDGVHIFFPDPWHKKRHNKRRLIQAPLVEKLAKKLK
PGGYFHAATDWEDYAIQILEVLNGNADLENTADGYAPRPDYRPLTKFEARGIKLGHGVWDVIFRRK

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=220, Percent_Identity=52.7272727272727, Blast_Score=241, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6320000, Length=171, Percent_Identity=29.8245614035088, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_CHRVO (Q7NRJ5)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903456.1
- ProteinModelPortal:   Q7NRJ5
- SMR:   Q7NRJ5
- GeneID:   2547830
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3786
- NMPDR:   fig|243365.1.peg.3786
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- PhylomeDB:   Q7NRJ5
- ProtClustDB:   PRK00121
- BioCyc:   CVIO243365:CV_3786-MONOMER
- BRENDA:   2.1.1.33
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 25380; Mature: 25380

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: NA

Important sites: BINDING 59-59 BINDING 84-84 BINDING 111-111 BINDING 134-134 BINDING 138-138 BINDING 170-170

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENPAFKRAIRSFVLRQGHLSAGQQRAMDEGMPKWGIEYRPETMDLEQVFGRAAPKILEI
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHH
GFGMGGATAEIAAANPDNDYLGIEVHGPGVGNLCKLIAEKELTNLRLMRHDAVEVLDNML
CCCCCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADGSLDGVHIFFPDPWHKKRHNKRRLIQAPLVEKLAKKLKPGGYFHAATDWEDYAIQILE
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHH
VLNGNADLENTADGYAPRPDYRPLTKFEARGIKLGHGVWDVIFRRK
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEECCHHHHHHHCCC
>Mature Secondary Structure
MENPAFKRAIRSFVLRQGHLSAGQQRAMDEGMPKWGIEYRPETMDLEQVFGRAAPKILEI
CCCHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCCCHHHHH
GFGMGGATAEIAAANPDNDYLGIEVHGPGVGNLCKLIAEKELTNLRLMRHDAVEVLDNML
CCCCCCCCEEEEECCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ADGSLDGVHIFFPDPWHKKRHNKRRLIQAPLVEKLAKKLKPGGYFHAATDWEDYAIQILE
CCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCHHHHHHHHHH
VLNGNADLENTADGYAPRPDYRPLTKFEARGIKLGHGVWDVIFRRK
HHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCEEECCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782