Click here to switch to the map view.

The map label for this gene is dnaG [H]

Identifier: 34499218

GI number: 34499218

Start: 4062014

End: 4063810

Strand: Reverse

Name: dnaG [H]

Synonym: CV_3763

Alternate gene names: 34499218

Gene position: 4063810-4062014 (Counterclockwise)

Preceding gene: 34499219

Following gene: 34499217

Centisome position: 85.53

GC content: 65.22

Gene sequence:

>1797_bases
TTGATTCCGCAGGATTTCATCGACCAGCTGTTGTCCCGCGTCGACATCGTCGACGTGGTGGACCGCTACGTCCCCTTGAA
GAAGGGCGGCCAGAACTACATGGCTTGCTGCCCCTTTCACAAGGAAAAATCGCCCTCATTCACCGTCAGTCCCAGCAAGC
AGTTCTATCATTGCTTCGGCTGCGGCGCGCACGGCTCGGCCATCGGCTTCGTGATGGAGTACCAGGGCATCGGCTTCGTC
GATGCCGTCAAGATGCTGGCCGAAGGCATCGGCATGCAGGTGCCGAACGAGCGCAGCGTCAACCCGGAAGCCAGCCGCAA
GGCGCGCGAGAAGCAGGTGTCGCTGGAGCAGCTGATGCAGCTTGCCAGCGACTTCTACCGCCGCGAGCTGAAGGGGGCGG
CCAACGCCGTCGCCTATTGCAAGGGGCGCGGGCTGTCGGGCGAGATCGCCGCGCGCTTCGGCCTGGGCTATGCGCCGGAC
GGCTGGCAGAACCTTGAGGCCGCGGTAGACAACTACCAGGACGACAAGCTGGTGGAAGCCGGCCTGGTGATCGTGGCCGA
GGACAGCGGGCGACGCTACGACCGCTTCCGCGACCGGCTGATGTTCCCGATCCGCAACCAGCGCGGCGCCATCGTCGGCT
TCGGCGGGCGGGTGCTGGGCAAGGGCGAGCCGAAGTATCTGAACTCGCCGGAAACGCCGCTGTTCGAGAAGGGGCGCGAG
CTGTACGGCCTGTACGAGGGGCGGCAGGCGATCCGGGAGAAAAACCGCGTCCTGGTGGTCGAAGGCTATATGGACGTGGT
GGCGCTGGCCCAGTACGGCATCGGCTACGCGGTGGCCACGCTGGGCACCGCCACCACCGGCGAGCATGTGCGCAAGCTGC
TCAAGCACGCGGAGCAGGTGTATTTCTGCTTCGACGGCGACGCGGCCGGCCAGAAGGCCGCCTGGCGCGCGCTGGAGAAC
AGTTTGCCGCAGCTGGTGGACGGCAAGGCGCTGAACTTCCTGTTCCTGCCGACCGAGCACGATCCGGACAGTTATGTGCG
CGAATTCGGCGCCGATGCGTTTGAAGAGTTGCTGACCCAGCAGAGCCTGCCGCTGTCGGTGTATTTCACCCGCGAGTTGT
GCCGCGGCATCAACCTCGGCACGCCGGAGGGCAAGGCGGACCTGATCCGCCAGGCGACGCCGCTGCTGGCGCAGATCGCC
GCGCCGGCGCTGGGGTACATGATACGCAAGCGGCTGGCCGAGATGGCCGGCGTGGACGTCGACGAGCTGGACATGCTGAC
CGGCGGCAAGCCGCAGCGATCCGAGCGCGGCGGCCGCAGCGGCGGCCGGCGAGATTACAAAGTGCCGGCCGAGTCGCAGC
GCGTGGTCAACACCACCATGGTCAAGAAGCTGATCAAGTGGTTGTTGATGAACCCGCAATGGGCAAGCGACGTCAAGCTG
CCGGACAGCCTCGCGCTGTCGGACGAGTTGGGCTGTTTCGCGATGCTGGCCGAGCGGGTGCTGGAGCACGGCGACGCGCC
GAATACCGCTCAGTTGATCGAAGGATTGCGCGGCACGCCCTACGAGGGCTTGATAGACGGCGTGCTGCAGCAGGCGATGC
AGGATCCGGACGAGTTCGCCGATCCAAGCGACGATGACCGGCAGCTGTTCCAGGATGGCAACGACAGGCTGCTGAAAATG
CTGCATGCGGAGCAACTGGAGCGGCTAAAACTGAAGGACCGGCACGAAGGGCTGACTCCTGAGGAGAAAGCCCTGATGGG
CCAGCTGTTGCGGGAAATGTTCTCGCCGCCGAACTAA

Upstream 100 bases:

>100_bases
TGGGCGCGCTGCGCCCTCAACTGGCCGGCCGCGCCGACATGGCGCAGGTGTCCGCGCTGATCAAGGCCAAGCTGACCGCT
TGACGCTCGGGTAGCAGGGT

Downstream 100 bases:

>100_bases
CGGGTTCGGCGGAAAAGTTCAAGTGGTTGTGTTATAATCAACTGTTTTTTTCGGCTTTTTTTCTAGCAGGAAGCGAAATG
GCGGCCTCTCCCGAAAACCA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MIPQDFIDQLLSRVDIVDVVDRYVPLKKGGQNYMACCPFHKEKSPSFTVSPSKQFYHCFGCGAHGSAIGFVMEYQGIGFV
DAVKMLAEGIGMQVPNERSVNPEASRKAREKQVSLEQLMQLASDFYRRELKGAANAVAYCKGRGLSGEIAARFGLGYAPD
GWQNLEAAVDNYQDDKLVEAGLVIVAEDSGRRYDRFRDRLMFPIRNQRGAIVGFGGRVLGKGEPKYLNSPETPLFEKGRE
LYGLYEGRQAIREKNRVLVVEGYMDVVALAQYGIGYAVATLGTATTGEHVRKLLKHAEQVYFCFDGDAAGQKAAWRALEN
SLPQLVDGKALNFLFLPTEHDPDSYVREFGADAFEELLTQQSLPLSVYFTRELCRGINLGTPEGKADLIRQATPLLAQIA
APALGYMIRKRLAEMAGVDVDELDMLTGGKPQRSERGGRSGGRRDYKVPAESQRVVNTTMVKKLIKWLLMNPQWASDVKL
PDSLALSDELGCFAMLAERVLEHGDAPNTAQLIEGLRGTPYEGLIDGVLQQAMQDPDEFADPSDDDRQLFQDGNDRLLKM
LHAEQLERLKLKDRHEGLTPEEKALMGQLLREMFSPPN

Sequences:

>Translated_598_residues
MIPQDFIDQLLSRVDIVDVVDRYVPLKKGGQNYMACCPFHKEKSPSFTVSPSKQFYHCFGCGAHGSAIGFVMEYQGIGFV
DAVKMLAEGIGMQVPNERSVNPEASRKAREKQVSLEQLMQLASDFYRRELKGAANAVAYCKGRGLSGEIAARFGLGYAPD
GWQNLEAAVDNYQDDKLVEAGLVIVAEDSGRRYDRFRDRLMFPIRNQRGAIVGFGGRVLGKGEPKYLNSPETPLFEKGRE
LYGLYEGRQAIREKNRVLVVEGYMDVVALAQYGIGYAVATLGTATTGEHVRKLLKHAEQVYFCFDGDAAGQKAAWRALEN
SLPQLVDGKALNFLFLPTEHDPDSYVREFGADAFEELLTQQSLPLSVYFTRELCRGINLGTPEGKADLIRQATPLLAQIA
APALGYMIRKRLAEMAGVDVDELDMLTGGKPQRSERGGRSGGRRDYKVPAESQRVVNTTMVKKLIKWLLMNPQWASDVKL
PDSLALSDELGCFAMLAERVLEHGDAPNTAQLIEGLRGTPYEGLIDGVLQQAMQDPDEFADPSDDDRQLFQDGNDRLLKM
LHAEQLERLKLKDRHEGLTPEEKALMGQLLREMFSPPN
>Mature_598_residues
MIPQDFIDQLLSRVDIVDVVDRYVPLKKGGQNYMACCPFHKEKSPSFTVSPSKQFYHCFGCGAHGSAIGFVMEYQGIGFV
DAVKMLAEGIGMQVPNERSVNPEASRKAREKQVSLEQLMQLASDFYRRELKGAANAVAYCKGRGLSGEIAARFGLGYAPD
GWQNLEAAVDNYQDDKLVEAGLVIVAEDSGRRYDRFRDRLMFPIRNQRGAIVGFGGRVLGKGEPKYLNSPETPLFEKGRE
LYGLYEGRQAIREKNRVLVVEGYMDVVALAQYGIGYAVATLGTATTGEHVRKLLKHAEQVYFCFDGDAAGQKAAWRALEN
SLPQLVDGKALNFLFLPTEHDPDSYVREFGADAFEELLTQQSLPLSVYFTRELCRGINLGTPEGKADLIRQATPLLAQIA
APALGYMIRKRLAEMAGVDVDELDMLTGGKPQRSERGGRSGGRRDYKVPAESQRVVNTTMVKKLIKWLLMNPQWASDVKL
PDSLALSDELGCFAMLAERVLEHGDAPNTAQLIEGLRGTPYEGLIDGVLQQAMQDPDEFADPSDDDRQLFQDGNDRLLKM
LHAEQLERLKLKDRHEGLTPEEKALMGQLLREMFSPPN

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=602, Percent_Identity=43.5215946843854, Blast_Score=452, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016136
- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR013173
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 66499; Mature: 66499

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPQDFIDQLLSRVDIVDVVDRYVPLKKGGQNYMACCPFHKEKSPSFTVSPSKQFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCEEECCCHHEEEEEC
CGAHGSAIGFVMEYQGIGFVDAVKMLAEGIGMQVPNERSVNPEASRKAREKQVSLEQLMQ
CCCCCCHHEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LASDFYRRELKGAANAVAYCKGRGLSGEIAARFGLGYAPDGWQNLEAAVDNYQDDKLVEA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHC
GLVIVAEDSGRRYDRFRDRLMFPIRNQRGAIVGFGGRVLGKGEPKYLNSPETPLFEKGRE
CEEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEECCEEECCCCCCCCCCCCCCHHHCCHH
LYGLYEGRQAIREKNRVLVVEGYMDVVALAQYGIGYAVATLGTATTGEHVRKLLKHAEQV
HHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHE
YFCFDGDAAGQKAAWRALENSLPQLVDGKALNFLFLPTEHDPDSYVREFGADAFEELLTQ
EEEECCCCCCHHHHHHHHHHCCHHHHCCCCEEEEEECCCCCHHHHHHHHCHHHHHHHHHH
QSLPLSVYFTRELCRGINLGTPEGKADLIRQATPLLAQIAAPALGYMIRKRLAEMAGVDV
CCCCEEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DELDMLTGGKPQRSERGGRSGGRRDYKVPAESQRVVNTTMVKKLIKWLLMNPQWASDVKL
HHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PDSLALSDELGCFAMLAERVLEHGDAPNTAQLIEGLRGTPYEGLIDGVLQQAMQDPDEFA
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHC
DPSDDDRQLFQDGNDRLLKMLHAEQLERLKLKDRHEGLTPEEKALMGQLLREMFSPPN
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIPQDFIDQLLSRVDIVDVVDRYVPLKKGGQNYMACCPFHKEKSPSFTVSPSKQFYHCFG
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCCCCEEECCCHHEEEEEC
CGAHGSAIGFVMEYQGIGFVDAVKMLAEGIGMQVPNERSVNPEASRKAREKQVSLEQLMQ
CCCCCCHHEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHH
LASDFYRRELKGAANAVAYCKGRGLSGEIAARFGLGYAPDGWQNLEAAVDNYQDDKLVEA
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHC
GLVIVAEDSGRRYDRFRDRLMFPIRNQRGAIVGFGGRVLGKGEPKYLNSPETPLFEKGRE
CEEEEECCCCCHHHHHHHHHCCCCCCCCCCEEEECCEEECCCCCCCCCCCCCCHHHCCHH
LYGLYEGRQAIREKNRVLVVEGYMDVVALAQYGIGYAVATLGTATTGEHVRKLLKHAEQV
HHHHHHHHHHHHHCCCEEEEECHHHHHHHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHE
YFCFDGDAAGQKAAWRALENSLPQLVDGKALNFLFLPTEHDPDSYVREFGADAFEELLTQ
EEEECCCCCCHHHHHHHHHHCCHHHHCCCCEEEEEECCCCCHHHHHHHHCHHHHHHHHHH
QSLPLSVYFTRELCRGINLGTPEGKADLIRQATPLLAQIAAPALGYMIRKRLAEMAGVDV
CCCCEEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCH
DELDMLTGGKPQRSERGGRSGGRRDYKVPAESQRVVNTTMVKKLIKWLLMNPQWASDVKL
HHHHHHCCCCCCHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
PDSLALSDELGCFAMLAERVLEHGDAPNTAQLIEGLRGTPYEGLIDGVLQQAMQDPDEFA
CCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHCCCHHHC
DPSDDDRQLFQDGNDRLLKMLHAEQLERLKLKDRHEGLTPEEKALMGQLLREMFSPPN
CCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]