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The map label for this gene is rlmL [H]

Identifier: 34499160

GI number: 34499160

Start: 4006140

End: 4007333

Strand: Direct

Name: rlmL [H]

Synonym: CV_3705

Alternate gene names: 34499160

Gene position: 4006140-4007333 (Clockwise)

Preceding gene: 34499144

Following gene: 34499161

Centisome position: 84.32

GC content: 70.77

Gene sequence:

>1194_bases
CTGGACAGCCGGCTGGCCATGTTCGCGCCCTGCCCGCGCGGCCTGGAGCAAATCCTGGCCGACGAGTTGCTGGCGCTGGG
CGCCGGCGACATCGCCCCGGCCGACGGCGGCGTGGCTTTCGCCGGCGACGCCAGGCTGATGATGGCGGTCAACCTGCACT
CGCGCACCGCCAGCCGCGTGCTGCTGCGCCTGGCCCACGGCGGCTATCACGCCGAGCAGGACATCTACCGGCTGGCGATG
AGCGTGGACTGGCCGCGCTGGTTCGAGGTGTCGCGCACCATCAAGCTGAAGGCCGACGGCATCGCCGCCCGCGTCAAGAG
CCTGGACTACATCGGCTTGACGGTGAAGGACGCGATCTGCGACCGCTTCCGCCAGGCGCACCTCGGCCGCCCCAGCGTCG
ACACCCGCCACCCGGACGTGCGCGTCAACGTGTTCCTCACCGCCGACGAAGCGACCATCTACCTCGACACCAGCGGCGAG
CCGCTATTCAAGCGCGGCTGGCGCGAGGAAACCGGCGAGGCGCCGCTGCGCGAAAACCTCGCCGCCGGCATCCTGCTCTT
GGCCGGCTACGACGGCAGCCAGCCGCTGCTGGACCCGATGTGCGGCAGCGGCACCTTCCTGGTGGAAGCCGCCGACATCG
CGCTGAACCGCGCGCCGGGCCGCGCCCGCCGCTTCGGCTTCGAGGCGCTGTCTAGCTTCGACTCCGCCGCCTGGGAAAAG
CTGCAGCAGGACGCGCGCAAGGCCGAGCAGCCGGCGTCCTCTCTGGCCATCCGCGGCTCCGACCGCTCGCAGGCCATGGT
CAATATCGCCCGCGCCAACCTGGAGCGCGCCGGCCTGGCCGAGCTGGTGGAAGTCAGCGCCGCCGACGTGACCGCCTCCC
GCCCGCACGCCGAGCACGGCCTGATCGTCAGCAACCCGCCGTACGGCGTGCGGCTGGAAGAGCAGGACCAGCTGGCGGCC
TGGTACCCGGAGCTGGGCGACTGGCTGAAGGCCCACTTCGCCGGCTGGACCGCCTGCCTGTTCAGCGGCGATCTGCGCCT
GGGCAAGCTGATCCGGCTGGCGCCCAAGCGCCGCACGCCGCTGTTCAACGGCTCGCTGCAATGCCGGCTGTTCGTCATCA
ACATGGTGGAAGGCAGCGCGCGCAAGCAGAAGGACGGCGACGAAGCCGAAATCGAAACCGGCGACGAACAGTAA

Upstream 100 bases:

>100_bases
CACCCACCGGGTCGACCAGCCGCGCCGCGCCTTCGAGCAGCGCCCGCACGACAGCAGCCACGCGCCGGCGCCCCGCGCGG
AAAACGTGATCACCAACCGC

Downstream 100 bases:

>100_bases
ACCCCCGCTTTCACGGCGACACGCCGCCCCGCCTCCGGCCGGGCGGCGTTTTTACGGACGTTCGCCGGCAAACATCGCGA
AAACGCTTAATTTCCAGTCG

Product: oxidoreductase

Products: NA

Alternate protein names: 23S rRNA m2G2445 methyltransferase; rRNA (guanine-N(2)-)-methyltransferase rlmL [H]

Number of amino acids: Translated: 397; Mature: 397

Protein sequence:

>397_residues
MDSRLAMFAPCPRGLEQILADELLALGAGDIAPADGGVAFAGDARLMMAVNLHSRTASRVLLRLAHGGYHAEQDIYRLAM
SVDWPRWFEVSRTIKLKADGIAARVKSLDYIGLTVKDAICDRFRQAHLGRPSVDTRHPDVRVNVFLTADEATIYLDTSGE
PLFKRGWREETGEAPLRENLAAGILLLAGYDGSQPLLDPMCGSGTFLVEAADIALNRAPGRARRFGFEALSSFDSAAWEK
LQQDARKAEQPASSLAIRGSDRSQAMVNIARANLERAGLAELVEVSAADVTASRPHAEHGLIVSNPPYGVRLEEQDQLAA
WYPELGDWLKAHFAGWTACLFSGDLRLGKLIRLAPKRRTPLFNGSLQCRLFVINMVEGSARKQKDGDEAEIETGDEQ

Sequences:

>Translated_397_residues
MDSRLAMFAPCPRGLEQILADELLALGAGDIAPADGGVAFAGDARLMMAVNLHSRTASRVLLRLAHGGYHAEQDIYRLAM
SVDWPRWFEVSRTIKLKADGIAARVKSLDYIGLTVKDAICDRFRQAHLGRPSVDTRHPDVRVNVFLTADEATIYLDTSGE
PLFKRGWREETGEAPLRENLAAGILLLAGYDGSQPLLDPMCGSGTFLVEAADIALNRAPGRARRFGFEALSSFDSAAWEK
LQQDARKAEQPASSLAIRGSDRSQAMVNIARANLERAGLAELVEVSAADVTASRPHAEHGLIVSNPPYGVRLEEQDQLAA
WYPELGDWLKAHFAGWTACLFSGDLRLGKLIRLAPKRRTPLFNGSLQCRLFVINMVEGSARKQKDGDEAEIETGDEQ
>Mature_397_residues
MDSRLAMFAPCPRGLEQILADELLALGAGDIAPADGGVAFAGDARLMMAVNLHSRTASRVLLRLAHGGYHAEQDIYRLAM
SVDWPRWFEVSRTIKLKADGIAARVKSLDYIGLTVKDAICDRFRQAHLGRPSVDTRHPDVRVNVFLTADEATIYLDTSGE
PLFKRGWREETGEAPLRENLAAGILLLAGYDGSQPLLDPMCGSGTFLVEAADIALNRAPGRARRFGFEALSSFDSAAWEK
LQQDARKAEQPASSLAIRGSDRSQAMVNIARANLERAGLAELVEVSAADVTASRPHAEHGLIVSNPPYGVRLEEQDQLAA
WYPELGDWLKAHFAGWTACLFSGDLRLGKLIRLAPKRRTPLFNGSLQCRLFVINMVEGSARKQKDGDEAEIETGDEQ

Specific function: Specifically methylates the guanosine in position 2445 (m2G2445) of 23S rRNA [H]

COG id: COG0116

COG function: function code L; Predicted N6-adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain [H]

Homologues:

Organism=Escherichia coli, GI1787181, Length=371, Percent_Identity=39.0835579514825, Blast_Score=235, Evalue=3e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR000241
- InterPro:   IPR017244
- InterPro:   IPR019614
- InterPro:   IPR004114 [H]

Pfam domain/function: PF10672 Methyltrans_SAM; PF02926 THUMP; PF01170 UPF0020 [H]

EC number: =2.1.1.173 [H]

Molecular weight: Translated: 43433; Mature: 43433

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS51165 THUMP ; PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSRLAMFAPCPRGLEQILADELLALGAGDIAPADGGVAFAGDARLMMAVNLHSRTASRV
CCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEECCHHHHHHH
LLRLAHGGYHAEQDIYRLAMSVDWPRWFEVSRTIKLKADGIAARVKSLDYIGLTVKDAIC
HHHHHCCCCCHHHHHHHHHHHCCCCHHEEECEEEEEECCCHHHHHHCCEEECEEHHHHHH
DRFRQAHLGRPSVDTRHPDVRVNVFLTADEATIYLDTSGEPLFKRGWREETGEAPLRENL
HHHHHHHCCCCCCCCCCCCEEEEEEEEECCEEEEEECCCCHHHHCCCCCCCCCCCHHHHH
AAGILLLAGYDGSQPLLDPMCGSGTFLVEAADIALNRAPGRARRFGFEALSSFDSAAWEK
HCCEEEEECCCCCCCCCCCCCCCCEEEEEEEHHHEECCCCHHHHHHHHHHHHHHHHHHHH
LQQDARKAEQPASSLAIRGSDRSQAMVNIARANLERAGLAELVEVSAADVTASRPHAEHG
HHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
LIVSNPPYGVRLEEQDQLAAWYPELGDWLKAHFAGWTACLFSGDLRLGKLIRLAPKRRTP
EEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHCCCCCCC
LFNGSLQCRLFVINMVEGSARKQKDGDEAEIETGDEQ
CCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDSRLAMFAPCPRGLEQILADELLALGAGDIAPADGGVAFAGDARLMMAVNLHSRTASRV
CCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEEECCHHHHHHH
LLRLAHGGYHAEQDIYRLAMSVDWPRWFEVSRTIKLKADGIAARVKSLDYIGLTVKDAIC
HHHHHCCCCCHHHHHHHHHHHCCCCHHEEECEEEEEECCCHHHHHHCCEEECEEHHHHHH
DRFRQAHLGRPSVDTRHPDVRVNVFLTADEATIYLDTSGEPLFKRGWREETGEAPLRENL
HHHHHHHCCCCCCCCCCCCEEEEEEEEECCEEEEEECCCCHHHHCCCCCCCCCCCHHHHH
AAGILLLAGYDGSQPLLDPMCGSGTFLVEAADIALNRAPGRARRFGFEALSSFDSAAWEK
HCCEEEEECCCCCCCCCCCCCCCCEEEEEEEHHHEECCCCHHHHHHHHHHHHHHHHHHHH
LQQDARKAEQPASSLAIRGSDRSQAMVNIARANLERAGLAELVEVSAADVTASRPHAEHG
HHHHHHHHHCCCHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
LIVSNPPYGVRLEEQDQLAAWYPELGDWLKAHFAGWTACLFSGDLRLGKLIRLAPKRRTP
EEECCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHEEEEEECCCCHHHHHHHCCCCCCC
LFNGSLQCRLFVINMVEGSARKQKDGDEAEIETGDEQ
CCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA