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The map label for this gene is pcm1

Identifier: 34499135

GI number: 34499135

Start: 3981235

End: 3981903

Strand: Direct

Name: pcm1

Synonym: CV_3680

Alternate gene names: 34499135

Gene position: 3981235-3981903 (Clockwise)

Preceding gene: 34499134

Following gene: 34499136

Centisome position: 83.8

GC content: 69.36

Gene sequence:

>669_bases
ATGGCTGCACCGCTGACCCCGAACGCCCAGGCCTACGGCATGCTTTCCGAGCGCACGCGCCGCCGCATGAGCGACCGCCT
GCGCCAGCACGGCATCAGCGACGAGCGGGTGCTGTCGGCGATGCACGAGGTGCCGCGCCACCTGTTCGTCGACCAGGCGC
TGGCCACCCGCGCCTATGACGACGTGTCGCTGCCCATCGGCCATGGCCAGACCATCTCCCAGCCGCTGACGGTGGCGAGG
ATGACCGAATTGCTGATCCAGGGCCGGCAACCCGGAAAGATCCTGGAGGTCGGCACCGGCTGCGGCTACCAGACGGCGGT
GCTGCTGAAAACCGGCGCCGAAGTCTATTCGATTGAACGACTCGGCGCGATTCTTGACCAAGCAAGACGCAATCTGCGCG
CCGCCAAGCTGGTCCACGCCCGGCTGGTGCACGGCGACGGCAACGCGGGCCTGCCCGAGGCCGCGCCGTTCGACGGCATC
ATCATCACCGCCGCCGCGCGCGCGGTGCCGGCGCCGCTGGTGGACCAGCTGGCCGAGGGCGGCCGGATGGTGCTGCCGCT
TGGCGACGCCGAGCAACACCTGTGGCTGCTGGAAAAAACGGCGCAAGGCCTGCAGAAGACCCGGCTGGATCCGGTCAAGT
TCGTTCCGCTGCTGGGCGGACGCGGCTGA

Upstream 100 bases:

>100_bases
GCGCCGGCACCGACTTCGGCTGCGTCGCGTCCAAGCAGGTGTCCGTCACGCCGCTGATGCTGGATCTGACCGCCTACGGG
CAACTGGACAGGATTTCCAC

Downstream 100 bases:

>100_bases
GGCGGCAAAAACGCAACACTTCAGCTGTCAGACAATCAAGCAAAACTCTTATTCAACGTTTGAACAAAAAAAACATGCTA
AAAATGCATTGCAGGTATGC

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MAAPLTPNAQAYGMLSERTRRRMSDRLRQHGISDERVLSAMHEVPRHLFVDQALATRAYDDVSLPIGHGQTISQPLTVAR
MTELLIQGRQPGKILEVGTGCGYQTAVLLKTGAEVYSIERLGAILDQARRNLRAAKLVHARLVHGDGNAGLPEAAPFDGI
IITAAARAVPAPLVDQLAEGGRMVLPLGDAEQHLWLLEKTAQGLQKTRLDPVKFVPLLGGRG

Sequences:

>Translated_222_residues
MAAPLTPNAQAYGMLSERTRRRMSDRLRQHGISDERVLSAMHEVPRHLFVDQALATRAYDDVSLPIGHGQTISQPLTVAR
MTELLIQGRQPGKILEVGTGCGYQTAVLLKTGAEVYSIERLGAILDQARRNLRAAKLVHARLVHGDGNAGLPEAAPFDGI
IITAAARAVPAPLVDQLAEGGRMVLPLGDAEQHLWLLEKTAQGLQKTRLDPVKFVPLLGGRG
>Mature_221_residues
AAPLTPNAQAYGMLSERTRRRMSDRLRQHGISDERVLSAMHEVPRHLFVDQALATRAYDDVSLPIGHGQTISQPLTVARM
TELLIQGRQPGKILEVGTGCGYQTAVLLKTGAEVYSIERLGAILDQARRNLRAAKLVHARLVHGDGNAGLPEAAPFDGII
ITAAARAVPAPLVDQLAEGGRMVLPLGDAEQHLWLLEKTAQGLQKTRLDPVKFVPLLGGRG

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Homo sapiens, GI226530908, Length=212, Percent_Identity=33.0188679245283, Blast_Score=80, Evalue=2e-15,
Organism=Escherichia coli, GI1789100, Length=207, Percent_Identity=46.3768115942029, Blast_Score=172, Evalue=2e-44,
Organism=Caenorhabditis elegans, GI71983477, Length=214, Percent_Identity=31.3084112149533, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI193207222, Length=213, Percent_Identity=30.5164319248826, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17981723, Length=210, Percent_Identity=34.2857142857143, Blast_Score=92, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_CHRVO (Q7NRV0)

Other databases:

- EMBL:   AE016825
- RefSeq:   NP_903350.1
- HSSP:   Q8TZR3
- ProteinModelPortal:   Q7NRV0
- SMR:   Q7NRV0
- GeneID:   2548808
- GenomeReviews:   AE016825_GR
- KEGG:   cvi:CV_3680
- NMPDR:   fig|243365.1.peg.3680
- HOGENOM:   HBG699907
- OMA:   YSVERIR
- PhylomeDB:   Q7NRV0
- ProtClustDB:   CLSK2485645
- BioCyc:   CVIO243365:CV_3680-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 24009; Mature: 23878

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 73-73

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPLTPNAQAYGMLSERTRRRMSDRLRQHGISDERVLSAMHEVPRHLFVDQALATRAYD
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DVSLPIGHGQTISQPLTVARMTELLIQGRQPGKILEVGTGCGYQTAVLLKTGAEVYSIER
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEECCCCHHHHHHH
LGAILDQARRNLRAAKLVHARLVHGDGNAGLPEAAPFDGIIITAAARAVPAPLVDQLAEG
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEHHHHHCCHHHHHHHHCC
GRMVLPLGDAEQHLWLLEKTAQGLQKTRLDPVKFVPLLGGRG
CEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHEECCCCCCC
>Mature Secondary Structure 
AAPLTPNAQAYGMLSERTRRRMSDRLRQHGISDERVLSAMHEVPRHLFVDQALATRAYD
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
DVSLPIGHGQTISQPLTVARMTELLIQGRQPGKILEVGTGCGYQTAVLLKTGAEVYSIER
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCEEEEECCCCHHHHHHH
LGAILDQARRNLRAAKLVHARLVHGDGNAGLPEAAPFDGIIITAAARAVPAPLVDQLAEG
HHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEHHHHHCCHHHHHHHHCC
GRMVLPLGDAEQHLWLLEKTAQGLQKTRLDPVKFVPLLGGRG
CEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782