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The map label for this gene is purN [H]

Identifier: 34499071

GI number: 34499071

Start: 3921189

End: 3921830

Strand: Direct

Name: purN [H]

Synonym: CV_3616

Alternate gene names: 34499071

Gene position: 3921189-3921830 (Clockwise)

Preceding gene: 34499070

Following gene: 34499072

Centisome position: 82.53

GC content: 69.78

Gene sequence:

>642_bases
ATGAAAAACATCGTCATCCTGATTTCCGGCCGCGGCTCCAATATGCAGGCCATCGTCGAGGCCGGCATTCCCGGCGCCCG
CGTCGCCGCGGTGATCGCCAACCGCCCGGACGCGGCCGGCCTCGCCTGGGCGGCCGAGCGCGGCATCGCCACGGCGGCGC
TGGACCACAAGGCCTACGCCAGCCGCGAGGCCTTCGACGCCGCGCTGGCCGCAGCGATAGACGCGCATCAGCCCGACCTG
GTGGTGCTGGCCGGCTTCATGCGCATCCTGACCGAAGGCTTCACCCGCCGCTACGAAGGCCGGATGATGAACATCCATCC
GTCGCTGCTGCCGGCCTTCCCCGGCCTGCACACTCACGAGCGCGCGCTGGAAATGGGCTGCAAACTGGCCGGATGCACGG
TACACTTCGTCACCGCCGAGCTCGATCACGGTCCCATCGTCGCCCAGGGCGCGGTGAATGTGCTGGACGGCGACACGCCG
GACAGCCTGGCCGCCCGGGTGTTGAAGCTGGAGCACCAACTGTACCCGGAAGCGGTGCGCCGCTTCGTCGCCGGCGAGAT
CGCCGTCGTCGACGGCAAAGTGGCCGCCGGCCCGGGAGCCGCCGCCAGCCTGCTGTCGCCCCTACCCTCAAGCAAGGCCT
AA

Upstream 100 bases:

>100_bases
CTGGCTCCGCCGGCGGCGCTTGTCGTTTATCGCCGGGCGGCGGAACCCAGCCGGCGCAATCGCAACAAACAGGAATGGCA
AGCCATTCCGGACATGAGCC

Downstream 100 bases:

>100_bases
ATGACCCGTCGCCGTCTGCTGCTGATCGCCCTTGTCCTGTCGTTGCTGCTGCACCTCGCGTTGCTCGGCTCCGACTTGCT
GCCCGCGATCAGCGCGCCGC

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MKNIVILISGRGSNMQAIVEAGIPGARVAAVIANRPDAAGLAWAAERGIATAALDHKAYASREAFDAALAAAIDAHQPDL
VVLAGFMRILTEGFTRRYEGRMMNIHPSLLPAFPGLHTHERALEMGCKLAGCTVHFVTAELDHGPIVAQGAVNVLDGDTP
DSLAARVLKLEHQLYPEAVRRFVAGEIAVVDGKVAAGPGAAASLLSPLPSSKA

Sequences:

>Translated_213_residues
MKNIVILISGRGSNMQAIVEAGIPGARVAAVIANRPDAAGLAWAAERGIATAALDHKAYASREAFDAALAAAIDAHQPDL
VVLAGFMRILTEGFTRRYEGRMMNIHPSLLPAFPGLHTHERALEMGCKLAGCTVHFVTAELDHGPIVAQGAVNVLDGDTP
DSLAARVLKLEHQLYPEAVRRFVAGEIAVVDGKVAAGPGAAASLLSPLPSSKA
>Mature_213_residues
MKNIVILISGRGSNMQAIVEAGIPGARVAAVIANRPDAAGLAWAAERGIATAALDHKAYASREAFDAALAAAIDAHQPDL
VVLAGFMRILTEGFTRRYEGRMMNIHPSLLPAFPGLHTHERALEMGCKLAGCTVHFVTAELDHGPIVAQGAVNVLDGDTP
DSLAARVLKLEHQLYPEAVRRFVAGEIAVVDGKVAAGPGAAASLLSPLPSSKA

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=196, Percent_Identity=42.3469387755102, Blast_Score=164, Evalue=7e-41,
Organism=Homo sapiens, GI209869995, Length=196, Percent_Identity=42.3469387755102, Blast_Score=164, Evalue=7e-41,
Organism=Homo sapiens, GI209869993, Length=196, Percent_Identity=42.3469387755102, Blast_Score=164, Evalue=7e-41,
Organism=Escherichia coli, GI1788846, Length=211, Percent_Identity=49.7630331753554, Blast_Score=187, Evalue=4e-49,
Organism=Escherichia coli, GI1787483, Length=191, Percent_Identity=32.9842931937173, Blast_Score=98, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=41.340782122905, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6320616, Length=212, Percent_Identity=31.1320754716981, Blast_Score=83, Evalue=3e-17,
Organism=Drosophila melanogaster, GI24582400, Length=189, Percent_Identity=46.031746031746, Blast_Score=171, Evalue=4e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 22196; Mature: 22196

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIVILISGRGSNMQAIVEAGIPGARVAAVIANRPDAAGLAWAAERGIATAALDHKAYA
CCEEEEEEECCCCCHHHHEECCCCCHHEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHH
SREAFDAALAAAIDAHQPDLVVLAGFMRILTEGFTRRYEGRMMNIHPSLLPAFPGLHTHE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHCCCCCCHHH
RALEMGCKLAGCTVHFVTAELDHGPIVAQGAVNVLDGDTPDSLAARVLKLEHQLYPEAVR
HHHHHCCEEECCEEEEEEEECCCCCEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
RFVAGEIAVVDGKVAAGPGAAASLLSPLPSSKA
HHHCCCEEEECCCCCCCCCHHHHHHCCCCCCCC
>Mature Secondary Structure
MKNIVILISGRGSNMQAIVEAGIPGARVAAVIANRPDAAGLAWAAERGIATAALDHKAYA
CCEEEEEEECCCCCHHHHEECCCCCHHEEEEECCCCCCCCHHHHHHCCCHHHHHHHHHHH
SREAFDAALAAAIDAHQPDLVVLAGFMRILTEGFTRRYEGRMMNIHPSLLPAFPGLHTHE
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECHHHHHCCCCCCHHH
RALEMGCKLAGCTVHFVTAELDHGPIVAQGAVNVLDGDTPDSLAARVLKLEHQLYPEAVR
HHHHHCCEEECCEEEEEEEECCCCCEEECCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
RFVAGEIAVVDGKVAAGPGAAASLLSPLPSSKA
HHHCCCEEEECCCCCCCCCHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3301838; 9205837; 9278503; 10954745; 2204419; 1522592; 1631098; 9698564; 10606510 [H]