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The map label for this gene is glnA [H]

Identifier: 34499044

GI number: 34499044

Start: 3896941

End: 3898359

Strand: Direct

Name: glnA [H]

Synonym: CV_3589

Alternate gene names: 34499044

Gene position: 3896941-3898359 (Clockwise)

Preceding gene: 34499037

Following gene: 34499045

Centisome position: 82.02

GC content: 64.2

Gene sequence:

>1419_bases
ATGGCAGTAGCCGACGTAGTCAAACTGATCCAAGAAAACGACGTCAAATTTGTCGATCTGCGCTTTACCGATACCCGCGG
CAAAGAACAGCACGTCTCCATCCCTGCGCACGTGCTGCTGGAAGACGCCGATGCCTGGTTCGAGCGCGGCCACGCCTTCG
ACGGCTCCTCCATCGCCGGCTGGAAAGGCATCCAGGCCTCCGACATGCTGCTGATCGCCGATCCGGCCACCGCCCGCATC
GACCCGTTCTTTGACGAGCCGACCGTGTTCATGCACTGCGACGTGATCGATCCGGCCGACGGCAAGGGCTACGACCGCGA
CCCGCGCTCCATCGCCAAGCGCGCCGAAGCCTACCTGAAGGCTTCCGGCCACGGCGACACCGCCTACTTCGGTCCGGAAC
CGGAATTCTTCATCTTCGACGGCGTGACCTGGGGCACCGACATGTCCGGCTGCTTCGTCAAGATCAAGGCCGAGGAAGCC
GCCTGGGCGTCCGCCGAGGAATTCGAAGGCGGCAACATGGGCCACCGCCCGGGCATCAAGGGCGGCTACTTCCCGGTTCC
GCCGGTGGACTCTTCGCAGGACATCCGCTCCGCGATGGTGCTGCTGCTGGAAGAGCTGGGCGTGCCGGTCGAAGTGCACC
ACCACGAAGTGGCCACCGCCGGCCAGAACGAAATCGGCACCAAGTTCAGCACGCTGACCCAGCGCGCCGACTGGACCCAG
ATCCTGAAGTACGTGGTGCACAACGTCGCCCACAGCTACGGCAAGACCGCCACCTTCATGCCCAAGCCCATCGTCGGCGA
CAACGGCTCCGGCATGCACGTGCACCAGTCGATCTGGAAGGACGGCAAGAACCTGTTCGCCGGCGAAGGCTACGCCGGCC
TGTCCGACACCGCGCTGTACTACATCGGCGGCATCATCAAGCACGCCAAGGCGCTGAACGCCATCACCAACCCGGGCACC
AACTCCTACAAGCGCCTGGTGCCGCATTACGAAGCGCCGGTGAAGCTGGCTTACTCCGCCAAGAACCGCTCCGCCTCGAT
CCGCATTCCGCACGTGGCCAGCCCGAAGGCCCGCCGCATCGAGGCCCGCTTCCCGGATCCGCTGGCCAACCCGTACCTGT
GCTTCTCCGCGCTGCTGATGGCCGGCCTGGACGGCATCCAGAACAAGATCCACCCGGGCGACGCCGCCGACAAGAATCTG
TACGACCTGCCGCCGGAAGAAGACAAGCTGATCCCGACCGTCTGCGCCTCGCTGGATGAAGCTCTGGCCGCGCTGGACAA
GGACCGCGAATTCCTGACCCGCGGCGGCGTGTTCTCCAACGAGTGGATCGACGCCTACATCGAGCTGAAGATGCAGGAAG
TCAACCTGACCCGCATGACCACCCACCCGGTGGAATTCGCGATGTACTACTCGCTGTAA

Upstream 100 bases:

>100_bases
AGCAGCGCTTCAGAGGCTGGCACGCTTCCTGCTTAACGGGACGTACCAAAATTTTCACGCACTTGCCCTTGGGTGCACGT
TGCCTTTCAGGAGATAAAAC

Downstream 100 bases:

>100_bases
GCGGCAAGCGCGTCGATCACAACGGCAGCCCAGGCTGCCGTTTTTCATGCCCTGCCGGCATGCTGGCATGAATGACGCGG
CGGCGCGATTTGCTTATCAT

Product: glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MAVADVVKLIQENDVKFVDLRFTDTRGKEQHVSIPAHVLLEDADAWFERGHAFDGSSIAGWKGIQASDMLLIADPATARI
DPFFDEPTVFMHCDVIDPADGKGYDRDPRSIAKRAEAYLKASGHGDTAYFGPEPEFFIFDGVTWGTDMSGCFVKIKAEEA
AWASAEEFEGGNMGHRPGIKGGYFPVPPVDSSQDIRSAMVLLLEELGVPVEVHHHEVATAGQNEIGTKFSTLTQRADWTQ
ILKYVVHNVAHSYGKTATFMPKPIVGDNGSGMHVHQSIWKDGKNLFAGEGYAGLSDTALYYIGGIIKHAKALNAITNPGT
NSYKRLVPHYEAPVKLAYSAKNRSASIRIPHVASPKARRIEARFPDPLANPYLCFSALLMAGLDGIQNKIHPGDAADKNL
YDLPPEEDKLIPTVCASLDEALAALDKDREFLTRGGVFSNEWIDAYIELKMQEVNLTRMTTHPVEFAMYYSL

Sequences:

>Translated_472_residues
MAVADVVKLIQENDVKFVDLRFTDTRGKEQHVSIPAHVLLEDADAWFERGHAFDGSSIAGWKGIQASDMLLIADPATARI
DPFFDEPTVFMHCDVIDPADGKGYDRDPRSIAKRAEAYLKASGHGDTAYFGPEPEFFIFDGVTWGTDMSGCFVKIKAEEA
AWASAEEFEGGNMGHRPGIKGGYFPVPPVDSSQDIRSAMVLLLEELGVPVEVHHHEVATAGQNEIGTKFSTLTQRADWTQ
ILKYVVHNVAHSYGKTATFMPKPIVGDNGSGMHVHQSIWKDGKNLFAGEGYAGLSDTALYYIGGIIKHAKALNAITNPGT
NSYKRLVPHYEAPVKLAYSAKNRSASIRIPHVASPKARRIEARFPDPLANPYLCFSALLMAGLDGIQNKIHPGDAADKNL
YDLPPEEDKLIPTVCASLDEALAALDKDREFLTRGGVFSNEWIDAYIELKMQEVNLTRMTTHPVEFAMYYSL
>Mature_471_residues
AVADVVKLIQENDVKFVDLRFTDTRGKEQHVSIPAHVLLEDADAWFERGHAFDGSSIAGWKGIQASDMLLIADPATARID
PFFDEPTVFMHCDVIDPADGKGYDRDPRSIAKRAEAYLKASGHGDTAYFGPEPEFFIFDGVTWGTDMSGCFVKIKAEEAA
WASAEEFEGGNMGHRPGIKGGYFPVPPVDSSQDIRSAMVLLLEELGVPVEVHHHEVATAGQNEIGTKFSTLTQRADWTQI
LKYVVHNVAHSYGKTATFMPKPIVGDNGSGMHVHQSIWKDGKNLFAGEGYAGLSDTALYYIGGIIKHAKALNAITNPGTN
SYKRLVPHYEAPVKLAYSAKNRSASIRIPHVASPKARRIEARFPDPLANPYLCFSALLMAGLDGIQNKIHPGDAADKNLY
DLPPEEDKLIPTVCASLDEALAALDKDREFLTRGGVFSNEWIDAYIELKMQEVNLTRMTTHPVEFAMYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1790301, Length=472, Percent_Identity=67.1610169491525, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=178, Percent_Identity=31.4606741573034, Blast_Score=78, Evalue=1e-15,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 51957; Mature: 51826

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVADVVKLIQENDVKFVDLRFTDTRGKEQHVSIPAHVLLEDADAWFERGHAFDGSSIAG
CCHHHHHHHHHCCCCEEEEEEEECCCCCCCEECCCCEEEEECHHHHHHCCCCCCCCCCCC
WKGIQASDMLLIADPATARIDPFFDEPTVFMHCDVIDPADGKGYDRDPRSIAKRAEAYLK
CCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHEE
ASGHGDTAYFGPEPEFFIFDGVTWGTDMSGCFVKIKAEEAAWASAEEFEGGNMGHRPGIK
ECCCCCCCEECCCCCEEEECCEECCCCCCCEEEEEEECCCCCCCHHHCCCCCCCCCCCCC
GGYFPVPPVDSSQDIRSAMVLLLEELGVPVEVHHHEVATAGQNEIGTKFSTLTQRADWTQ
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCHHHHCCCCHHCCHHHHHHHCCCHHH
ILKYVVHNVAHSYGKTATFMPKPIVGDNGSGMHVHQSIWKDGKNLFAGEGYAGLSDTALY
HHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCCCHHHHH
YIGGIIKHAKALNAITNPGTNSYKRLVPHYEAPVKLAYSAKNRSASIRIPHVASPKARRI
HHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCHHHEE
EARFPDPLANPYLCFSALLMAGLDGIQNKIHPGDAADKNLYDLPPEEDKLIPTVCASLDE
ECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
ALAALDKDREFLTRGGVFSNEWIDAYIELKMQEVNLTRMTTHPVEFAMYYSL
HHHHHHCCHHHHHHCCCCCHHHHEEEEEEEEEEECEEEEECCCEEEEEEECC
>Mature Secondary Structure 
AVADVVKLIQENDVKFVDLRFTDTRGKEQHVSIPAHVLLEDADAWFERGHAFDGSSIAG
CHHHHHHHHHCCCCEEEEEEEECCCCCCCEECCCCEEEEECHHHHHHCCCCCCCCCCCC
WKGIQASDMLLIADPATARIDPFFDEPTVFMHCDVIDPADGKGYDRDPRSIAKRAEAYLK
CCCCCCCCEEEEECCCCCCCCCCCCCCEEEEEEEEECCCCCCCCCCCHHHHHHHHHHHEE
ASGHGDTAYFGPEPEFFIFDGVTWGTDMSGCFVKIKAEEAAWASAEEFEGGNMGHRPGIK
ECCCCCCCEECCCCCEEEECCEECCCCCCCEEEEEEECCCCCCCHHHCCCCCCCCCCCCC
GGYFPVPPVDSSQDIRSAMVLLLEELGVPVEVHHHEVATAGQNEIGTKFSTLTQRADWTQ
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEECCHHHHCCCCHHCCHHHHHHHCCCHHH
ILKYVVHNVAHSYGKTATFMPKPIVGDNGSGMHVHQSIWKDGKNLFAGEGYAGLSDTALY
HHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCHHHHHHHHCCCCEECCCCCCCCCHHHHH
YIGGIIKHAKALNAITNPGTNSYKRLVPHYEAPVKLAYSAKNRSASIRIPHVASPKARRI
HHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCEEEEEECCCCCCEEECCCCCCCHHHEE
EARFPDPLANPYLCFSALLMAGLDGIQNKIHPGDAADKNLYDLPPEEDKLIPTVCASLDE
ECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
ALAALDKDREFLTRGGVFSNEWIDAYIELKMQEVNLTRMTTHPVEFAMYYSL
HHHHHHCCHHHHHHCCCCCHHHHEEEEEEEEEEECEEEEECCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA